Identification and cloning of waaF (rfaF) from Bordetella pertussis and use to generate mutants of Bordetella spp. with deep rough lipopolysaccharide

A DNA locus from Bordetella pertussis capable of reconstituting lipopolysaccharide (LPS) O-antigen biosynthesis in Salmonella typhimurium SL3789 (rfaF511) has been isolated, by using selection with the antibiotic novobiocin. DNA within the locus encodes a protein with amino acid sequence similarity...

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Veröffentlicht in:	Journal of bacteriology 1998-01, Vol.180 (1), p.35-40
Hauptverfasser:	Allen, A G, Isobe, T, Maskell, D J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacteriology Bordetella - enzymology Bordetella - genetics Bordetella pertussis - enzymology Bordetella pertussis - genetics Cell Surfaces Cloning Cloning, Molecular Deoxyribonucleic acid DNA Genes, Bacterial - genetics Genetic Complementation Test Glycosyltransferases - genetics Lipopolysaccharides - biosynthesis Molecular biology Molecular Sequence Data Mutation - physiology O Antigens - biosynthesis Phenotype Sequence Analysis, DNA Species Specificity
Online-Zugang:	Volltext
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