Resolution and Reconstitution of Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase, the Enzyme System Responsible for the Formation of the Chlorophyll Isocyclic Ring

Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase, the system responsible for the formation of the chlorophyll isocyclic ring in developing cucumber (Cucumis sativus L. cv Beit Alpha) chloroplasts, was resolved into two enzymic components: a high-speed supernatant and a membrane pellet. This...

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Veröffentlicht in:	Plant physiology (Bethesda) 1984-07, Vol.75 (3), p.658-661
Hauptverfasser:	Wong, Yum-Shing, Castelfranco, Paul A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Biosynthesis Chlorophylls Chloroplasts Cucumbers Enzymes Esters Fundamental and applied biological sciences. Psychology Greening Heterocyclic compounds, pigments Other biological molecules Plants Plastids Pyridine nucleotides Tetrapyrrolic pigments
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Zusammenfassung:	Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase, the system responsible for the formation of the chlorophyll isocyclic ring in developing cucumber (Cucumis sativus L. cv Beit Alpha) chloroplasts, was resolved into two enzymic components: a high-speed supernatant and a membrane pellet. This reconstituted enzyme system required reduced pyridine nucleotide for activity.
ISSN:	0032-0889 1532-2548
DOI:	10.1104/pp.75.3.658