Auxin requirements of sycamore cells in suspension culture [Acer pseudoplatanus]
Sycamore (Acer pseudoplatanus L.) cell suspension cultures (strain OS) require 2,4-dichlorophenoxyacetic acid (2,4-D) in their culture medium for normal growth. If the 2,4-D is omitted, rates of cell division are dramatically reduced and cell lysis may occur. Despite this 'auxin requirement,�...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1983-04, Vol.71 (4), p.927-931 |
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| container_title | Plant physiology (Bethesda) |
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| creator | Moloney, M M Hall, J F Robinson, G M Elliott, M C |
| description | Sycamore (Acer pseudoplatanus L.) cell suspension cultures (strain OS) require 2,4-dichlorophenoxyacetic acid (2,4-D) in their culture medium for normal growth. If the 2,4-D is omitted, rates of cell division are dramatically reduced and cell lysis may occur. Despite this 'auxin requirement,' it has been shown by gas chromatography-mass spectrometry that the cells synthesize indol-3yl-acetic acid (IAA). Changes in free 2,4-D and IAA in the cells during a culture passage have been monitored. There is a rapid uptake of 2,4-D by the cells during the lag phase leading to a maximum concentration per cell (125 nanograms per 106 cells) on day 2 followed by a decline to 45 nanograms per 106 cells by day 9 (middle of linear phase). The initial concentration of IAA (0.08 nanograms per 106 cells) rises slowly to a peak of 1.4 nanograms per 106 cells by day 9 then decreases rapidly to 0.2 nanograms per 106 cells by day 15 (early declining phase) and 0.08 nanograms per 106 cells by day 23 (early stationary phase). |
| doi_str_mv | 10.1104/pp.71.4.927 |
| format | Article |
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If the 2,4-D is omitted, rates of cell division are dramatically reduced and cell lysis may occur. Despite this 'auxin requirement,' it has been shown by gas chromatography-mass spectrometry that the cells synthesize indol-3yl-acetic acid (IAA). Changes in free 2,4-D and IAA in the cells during a culture passage have been monitored. There is a rapid uptake of 2,4-D by the cells during the lag phase leading to a maximum concentration per cell (125 nanograms per 106 cells) on day 2 followed by a decline to 45 nanograms per 106 cells by day 9 (middle of linear phase). The initial concentration of IAA (0.08 nanograms per 106 cells) rises slowly to a peak of 1.4 nanograms per 106 cells by day 9 then decreases rapidly to 0.2 nanograms per 106 cells by day 15 (early declining phase) and 0.08 nanograms per 106 cells by day 23 (early stationary phase).</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.71.4.927</identifier><identifier>PMID: 16662930</identifier><language>eng</language><publisher>United States: American Society of Plant Physiologists</publisher><subject>Auxins ; Cell culture techniques ; Cell division ; Cell growth ; Chemical suspensions ; Cultured cells ; L cells ; Plant cells ; Plants ; Solvents</subject><ispartof>Plant physiology (Bethesda), 1983-04, Vol.71 (4), p.927-931</ispartof><rights>Copyright 1983 The American Society of Plant Physiologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-b149eb1a26dc4a3351be27ed797846837b8583cceb9e81780240ad92f073085b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4267953$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4267953$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27903,27904,57996,58229</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16662930$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moloney, M M</creatorcontrib><creatorcontrib>Hall, J F</creatorcontrib><creatorcontrib>Robinson, G M</creatorcontrib><creatorcontrib>Elliott, M C</creatorcontrib><creatorcontrib>Instituto Nacional de Investigaciones Agrarias, Madrid (Spain)</creatorcontrib><title>Auxin requirements of sycamore cells in suspension culture [Acer pseudoplatanus]</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Sycamore (Acer pseudoplatanus L.) cell suspension cultures (strain OS) require 2,4-dichlorophenoxyacetic acid (2,4-D) in their culture medium for normal growth. If the 2,4-D is omitted, rates of cell division are dramatically reduced and cell lysis may occur. Despite this 'auxin requirement,' it has been shown by gas chromatography-mass spectrometry that the cells synthesize indol-3yl-acetic acid (IAA). Changes in free 2,4-D and IAA in the cells during a culture passage have been monitored. There is a rapid uptake of 2,4-D by the cells during the lag phase leading to a maximum concentration per cell (125 nanograms per 106 cells) on day 2 followed by a decline to 45 nanograms per 106 cells by day 9 (middle of linear phase). The initial concentration of IAA (0.08 nanograms per 106 cells) rises slowly to a peak of 1.4 nanograms per 106 cells by day 9 then decreases rapidly to 0.2 nanograms per 106 cells by day 15 (early declining phase) and 0.08 nanograms per 106 cells by day 23 (early stationary phase).</description><subject>Auxins</subject><subject>Cell culture techniques</subject><subject>Cell division</subject><subject>Cell growth</subject><subject>Chemical suspensions</subject><subject>Cultured cells</subject><subject>L cells</subject><subject>Plant cells</subject><subject>Plants</subject><subject>Solvents</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><recordid>eNpVkUtr3DAUhUVpaCZpV92W4l0WYSa6kqzHpjCEviDQQJtVKUKWr1MH23IkqzT_vhpmSJqVBOfT0bn3EPIW6AaAiot53ijYiI1h6gVZQc3ZmtVCvyQrSsudam2OyUlKd5RS4CBekWOQUjLD6Ypcb_Pffqoi3uc-4ojTkqrQVenBuzFErDwOQ6oKkXKacUp9mCqfhyUX7efWY6zmhLkN8-AWN-X06zU56tyQ8M3hPCU3nz7-uPyyvvr2-evl9mrtuTbLugFhsAHHZOuF47yGBpnCVhmlhdRcNbrW3HtsDGpQmjJBXWtYRxWnum74Kfmw951zM2LrS_LoBjvHfnTxwQbX2-fK1P-2t-GPBSoliLoYnB0MYrjPmBY79mk3rpsw5GQV50JrKnfk-Z70MaQUsXv8BajdVWDn2SqwwpYKCv3-_2BP7GHnBXi3B-7SEuKjLphUpuZP7zsXrLuNfbI338FoQakAVoL_A-LOlfo</recordid><startdate>19830401</startdate><enddate>19830401</enddate><creator>Moloney, M M</creator><creator>Hall, J F</creator><creator>Robinson, G M</creator><creator>Elliott, M C</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19830401</creationdate><title>Auxin requirements of sycamore cells in suspension culture [Acer pseudoplatanus]</title><author>Moloney, M M ; Hall, J F ; Robinson, G M ; Elliott, M C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-b149eb1a26dc4a3351be27ed797846837b8583cceb9e81780240ad92f073085b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Auxins</topic><topic>Cell culture techniques</topic><topic>Cell division</topic><topic>Cell growth</topic><topic>Chemical suspensions</topic><topic>Cultured cells</topic><topic>L cells</topic><topic>Plant cells</topic><topic>Plants</topic><topic>Solvents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moloney, M M</creatorcontrib><creatorcontrib>Hall, J F</creatorcontrib><creatorcontrib>Robinson, G M</creatorcontrib><creatorcontrib>Elliott, M C</creatorcontrib><creatorcontrib>Instituto Nacional de Investigaciones Agrarias, Madrid (Spain)</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moloney, M M</au><au>Hall, J F</au><au>Robinson, G M</au><au>Elliott, M C</au><aucorp>Instituto Nacional de Investigaciones Agrarias, Madrid (Spain)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Auxin requirements of sycamore cells in suspension culture [Acer pseudoplatanus]</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1983-04-01</date><risdate>1983</risdate><volume>71</volume><issue>4</issue><spage>927</spage><epage>931</epage><pages>927-931</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>Sycamore (Acer pseudoplatanus L.) cell suspension cultures (strain OS) require 2,4-dichlorophenoxyacetic acid (2,4-D) in their culture medium for normal growth. If the 2,4-D is omitted, rates of cell division are dramatically reduced and cell lysis may occur. Despite this 'auxin requirement,' it has been shown by gas chromatography-mass spectrometry that the cells synthesize indol-3yl-acetic acid (IAA). Changes in free 2,4-D and IAA in the cells during a culture passage have been monitored. There is a rapid uptake of 2,4-D by the cells during the lag phase leading to a maximum concentration per cell (125 nanograms per 106 cells) on day 2 followed by a decline to 45 nanograms per 106 cells by day 9 (middle of linear phase). The initial concentration of IAA (0.08 nanograms per 106 cells) rises slowly to a peak of 1.4 nanograms per 106 cells by day 9 then decreases rapidly to 0.2 nanograms per 106 cells by day 15 (early declining phase) and 0.08 nanograms per 106 cells by day 23 (early stationary phase).</abstract><cop>United States</cop><pub>American Society of Plant Physiologists</pub><pmid>16662930</pmid><doi>10.1104/pp.71.4.927</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1983-04, Vol.71 (4), p.927-931 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1066145 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Jstor Complete Legacy; Alma/SFX Local Collection |
| subjects | Auxins Cell culture techniques Cell division Cell growth Chemical suspensions Cultured cells L cells Plant cells Plants Solvents |
| title | Auxin requirements of sycamore cells in suspension culture [Acer pseudoplatanus] |
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