Isolation and transport properties of protoplasts from cortical cells of corn roots [Zea mays, maize]

A procedure was developed for the enzymic isolation of large quantities of protoplasts from the cortex of Zea mays L. WF9 × MO 17 roots. Cortex was separated from the primary root, sectioned, and the cell walls digested for 3.5 hours in 2% (w/v) Cellulysin, 0.1% Pectolyase Y-23, 1 millimolar CaCl2,...

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Veröffentlicht in:	Plant physiology (Bethesda) 1982-11, Vol.70 (5), p.1391-1395
Hauptverfasser:	Gronwald, John W., Leonard, Robert T.
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell membranes Cell walls Chemical suspensions Corn cortex Ion transport ions Plant cells Plant roots Plant tissues Plants Protoplasts roots Zea mays
Online-Zugang:	Volltext
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