Poly(A) Polymerase Modification and Reverse Transcriptase PCR Amplification of Environmental RNA

We describe a combination of two established techniques for a novel application for constructing full-length cDNA clone libraries from environmental RNA. The cDNA was cloned without the use of prescribed primers that target specific genes, and the procedure did not involve random priming. Purified R...

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Veröffentlicht in:	Applied and Environmental Microbiology 2005-03, Vol.71 (3), p.1267-1275
Hauptverfasser:	Botero, Lina M, D'Imperio, Seth, Burr, Mark, McDermott, Timothy R, Young, Mark, Hassett, Daniel J
Format:	Artikel
Sprache:	eng
Schlagworte:	Archaea Archaea - genetics Archaea - isolation & purification Bacteria - genetics Bacteria - isolation & purification Base Sequence Biological and medical sciences cDNA libraries Cloning Deoxyribonucleic acid Diverse techniques DNA Environmental Microbiology enzyme activity Enzymes Evolution, Molecular Firmicutes Fundamental and applied biological sciences. Psychology Gene Library geothermal soils geothermally heated soils Halobacterium halobium Methods Microbiology Molecular and cellular biology Phylogeny Polynucleotide Adenylyltransferase Proteobacteria reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - methods ribosomal RNA RNA RNA polymerase RNA, Archaeal - genetics RNA, Archaeal - isolation & purification RNA, Bacterial - genetics RNA, Bacterial - isolation & purification RNA, Ribosomal, 16S - genetics soil bacteria Soil Microbiology Sulfolobus solfataricus thermophilic bacteria
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