Impact of maternally derived meiotic aneuploidies on early embryonic development in vitro
Purpose To assess early embryonic developmental potential of embryos affected by maternally inherited meiotic aneuploidies. Methods This observational, descriptive study includes 930 oocytes from 151 patients which were retrospectively analyzed by combining the morphological assessment with the gene...
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| Veröffentlicht in: | Journal of assisted reproduction and genetics 2023-11, Vol.40 (11), p.2715-2723 |
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| creator | Tschare, Lena Ennemoser, Anna Carli, Luca Vaccari, Enrico Feichtinger, Michael |
| description | Purpose
To assess early embryonic developmental potential of embryos affected by maternally inherited meiotic aneuploidies.
Methods
This observational, descriptive study includes 930 oocytes from 151 patients which were retrospectively analyzed by combining the morphological assessment with the genetic results from polar body diagnosis.
Results
Of 930 oocytes examined, 566 (60.9%) were tested aneuploid. Developmental potential until cleavage stage was not affected by trisomies or monosomies (69.6% vs. 77.1%,
p
= 0.75). However, trisomies significantly more often resulted in top quality cleavage stage embryos compared to monosomies (20% vs. 17.6%,
p
= |
| doi_str_mv | 10.1007/s10815-023-02922-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10643722</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2857853612</sourcerecordid><originalsourceid>FETCH-LOGICAL-c382t-9b1ff5eb455d1431be364d988910d5e762562cda7de4bdbf72cf429f0dc02123</originalsourceid><addsrcrecordid>eNp9kU9LHTEUxUOxVGv7BbqQgJtups3fSbISkdYKQjduugqZ5I6NzEzGZObB-_bN86m1LroICZzfPffeHIQ-UfKFEqK-Fko0lQ1hvB7DWGPeoCMqFW8U5-SgvonUDRGtPkTvS7kjhBjN-Dt0yFXLWcvNEfp1Nc7OLzj1eHQL5MkNwxYHyHEDAY8Q0xI9dhOs85BiiFBwmjC4XCkYu7xNU9UDbGBI8wjTguOEN3HJ6QN627uhwMfH-xjdfP92c_Gjuf55eXVxft14rtnSmI72vYROSBmo4LQD3opgtDaUBAmqZbJlPjgVQHSh6xXzvWCmJ8ETRhk_Rmd723ntRgi-jpDdYOccR5e3Nrlo_1Wm-Nvepo2lpBVcsZ3D50eHnO5XKIsdY_EwDHXrtBbLtFRa8vah2ekr9C6tuz_bUdqIGofQlWJ7yudUSob-eRpK7C45u0_O1uTsQ3LW1KKTl3s8lzxFVQG-B0qVplvIf3v_x_YPk--l1g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2889481548</pqid></control><display><type>article</type><title>Impact of maternally derived meiotic aneuploidies on early embryonic development in vitro</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>SpringerLink Journals - AutoHoldings</source><creator>Tschare, Lena ; Ennemoser, Anna ; Carli, Luca ; Vaccari, Enrico ; Feichtinger, Michael</creator><creatorcontrib>Tschare, Lena ; Ennemoser, Anna ; Carli, Luca ; Vaccari, Enrico ; Feichtinger, Michael</creatorcontrib><description>Purpose
To assess early embryonic developmental potential of embryos affected by maternally inherited meiotic aneuploidies.
Methods
This observational, descriptive study includes 930 oocytes from 151 patients which were retrospectively analyzed by combining the morphological assessment with the genetic results from polar body diagnosis.
Results
Of 930 oocytes examined, 566 (60.9%) were tested aneuploid. Developmental potential until cleavage stage was not affected by trisomies or monosomies (69.6% vs. 77.1%,
p
= 0.75). However, trisomies significantly more often resulted in top quality cleavage stage embryos compared to monosomies (20% vs. 17.6%,
p
= < 0.01). Top quality blastocysts were more likely to be euploid than aneuploid (52.4% vs. 47.6%,
p
= 0.032). Additionally, significantly more aneuploid embryos resulted in developmental arrest compared to euploid embryos (15.3% vs. 6.7%,
p
= 0.003). Overall, there was no significant difference in the frequency of trisomies and monosomies in blastocyst stage embryos. (28.3% vs. 28.2%;
p
= 0.81). In contrast to earlier developmental stages, distribution of trisomies and monosomies did not differ in top quality blastocysts (8.3% vs. 5.3%,
p
= 0.32). However, certain chromosomal abnormalities showed a higher potential to develop into a top-rated blastocyst. These included monosomies 2, 5, 8, 10, 16, 17, 20, 21, and 22 and trisomies 2, 4, 5, 8, 9, 10, 11, 12, 13, 16, 17, 18 and 20.
Conclusion
Meiotically induced maternal aneuploidies have different effects on early embryonic development. While no difference in developmental potential between monosomies and trisomies could be observed in blastocysts, cleavage stage quality was significantly affected by chromosomal aneuploidies.</description><identifier>ISSN: 1058-0468</identifier><identifier>ISSN: 1573-7330</identifier><identifier>EISSN: 1573-7330</identifier><identifier>DOI: 10.1007/s10815-023-02922-9</identifier><identifier>PMID: 37632639</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aneuploidy ; Biopsy ; Blastocyst ; Blastocysts ; Chromosome aberrations ; Developmental stages ; Embryogenesis ; Embryology ; Embryonic Development - genetics ; Embryos ; Female ; Fertility ; Follicles ; Genetic testing ; Genetics ; Gynecology ; Human Genetics ; Humans ; Infertility ; Medicine ; Medicine & Public Health ; Meiosis ; Monosomy ; Morphology ; Oocytes ; Pregnancy ; Preimplantation Diagnosis - methods ; Reproductive Medicine ; Retrospective Studies ; Sperm ; Trisomy ; Ultrasonic imaging</subject><ispartof>Journal of assisted reproduction and genetics, 2023-11, Vol.40 (11), p.2715-2723</ispartof><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023. corrected publication 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023, corrected publication 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c382t-9b1ff5eb455d1431be364d988910d5e762562cda7de4bdbf72cf429f0dc02123</cites><orcidid>0009-0000-3644-8286 ; 0000-0001-6453-9281</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643722/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643722/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27922,27923,41486,42555,51317,53789,53791</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37632639$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tschare, Lena</creatorcontrib><creatorcontrib>Ennemoser, Anna</creatorcontrib><creatorcontrib>Carli, Luca</creatorcontrib><creatorcontrib>Vaccari, Enrico</creatorcontrib><creatorcontrib>Feichtinger, Michael</creatorcontrib><title>Impact of maternally derived meiotic aneuploidies on early embryonic development in vitro</title><title>Journal of assisted reproduction and genetics</title><addtitle>J Assist Reprod Genet</addtitle><addtitle>J Assist Reprod Genet</addtitle><description>Purpose
To assess early embryonic developmental potential of embryos affected by maternally inherited meiotic aneuploidies.
Methods
This observational, descriptive study includes 930 oocytes from 151 patients which were retrospectively analyzed by combining the morphological assessment with the genetic results from polar body diagnosis.
Results
Of 930 oocytes examined, 566 (60.9%) were tested aneuploid. Developmental potential until cleavage stage was not affected by trisomies or monosomies (69.6% vs. 77.1%,
p
= 0.75). However, trisomies significantly more often resulted in top quality cleavage stage embryos compared to monosomies (20% vs. 17.6%,
p
= < 0.01). Top quality blastocysts were more likely to be euploid than aneuploid (52.4% vs. 47.6%,
p
= 0.032). Additionally, significantly more aneuploid embryos resulted in developmental arrest compared to euploid embryos (15.3% vs. 6.7%,
p
= 0.003). Overall, there was no significant difference in the frequency of trisomies and monosomies in blastocyst stage embryos. (28.3% vs. 28.2%;
p
= 0.81). In contrast to earlier developmental stages, distribution of trisomies and monosomies did not differ in top quality blastocysts (8.3% vs. 5.3%,
p
= 0.32). However, certain chromosomal abnormalities showed a higher potential to develop into a top-rated blastocyst. These included monosomies 2, 5, 8, 10, 16, 17, 20, 21, and 22 and trisomies 2, 4, 5, 8, 9, 10, 11, 12, 13, 16, 17, 18 and 20.
Conclusion
Meiotically induced maternal aneuploidies have different effects on early embryonic development. While no difference in developmental potential between monosomies and trisomies could be observed in blastocysts, cleavage stage quality was significantly affected by chromosomal aneuploidies.</description><subject>Aneuploidy</subject><subject>Biopsy</subject><subject>Blastocyst</subject><subject>Blastocysts</subject><subject>Chromosome aberrations</subject><subject>Developmental stages</subject><subject>Embryogenesis</subject><subject>Embryology</subject><subject>Embryonic Development - genetics</subject><subject>Embryos</subject><subject>Female</subject><subject>Fertility</subject><subject>Follicles</subject><subject>Genetic testing</subject><subject>Genetics</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Infertility</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Meiosis</subject><subject>Monosomy</subject><subject>Morphology</subject><subject>Oocytes</subject><subject>Pregnancy</subject><subject>Preimplantation Diagnosis - methods</subject><subject>Reproductive Medicine</subject><subject>Retrospective Studies</subject><subject>Sperm</subject><subject>Trisomy</subject><subject>Ultrasonic imaging</subject><issn>1058-0468</issn><issn>1573-7330</issn><issn>1573-7330</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU9LHTEUxUOxVGv7BbqQgJtups3fSbISkdYKQjduugqZ5I6NzEzGZObB-_bN86m1LroICZzfPffeHIQ-UfKFEqK-Fko0lQ1hvB7DWGPeoCMqFW8U5-SgvonUDRGtPkTvS7kjhBjN-Dt0yFXLWcvNEfp1Nc7OLzj1eHQL5MkNwxYHyHEDAY8Q0xI9dhOs85BiiFBwmjC4XCkYu7xNU9UDbGBI8wjTguOEN3HJ6QN627uhwMfH-xjdfP92c_Gjuf55eXVxft14rtnSmI72vYROSBmo4LQD3opgtDaUBAmqZbJlPjgVQHSh6xXzvWCmJ8ETRhk_Rmd723ntRgi-jpDdYOccR5e3Nrlo_1Wm-Nvepo2lpBVcsZ3D50eHnO5XKIsdY_EwDHXrtBbLtFRa8vah2ekr9C6tuz_bUdqIGofQlWJ7yudUSob-eRpK7C45u0_O1uTsQ3LW1KKTl3s8lzxFVQG-B0qVplvIf3v_x_YPk--l1g</recordid><startdate>20231101</startdate><enddate>20231101</enddate><creator>Tschare, Lena</creator><creator>Ennemoser, Anna</creator><creator>Carli, Luca</creator><creator>Vaccari, Enrico</creator><creator>Feichtinger, Michael</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0009-0000-3644-8286</orcidid><orcidid>https://orcid.org/0000-0001-6453-9281</orcidid></search><sort><creationdate>20231101</creationdate><title>Impact of maternally derived meiotic aneuploidies on early embryonic development in vitro</title><author>Tschare, Lena ; Ennemoser, Anna ; Carli, Luca ; Vaccari, Enrico ; Feichtinger, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-9b1ff5eb455d1431be364d988910d5e762562cda7de4bdbf72cf429f0dc02123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Aneuploidy</topic><topic>Biopsy</topic><topic>Blastocyst</topic><topic>Blastocysts</topic><topic>Chromosome aberrations</topic><topic>Developmental stages</topic><topic>Embryogenesis</topic><topic>Embryology</topic><topic>Embryonic Development - genetics</topic><topic>Embryos</topic><topic>Female</topic><topic>Fertility</topic><topic>Follicles</topic><topic>Genetic testing</topic><topic>Genetics</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Infertility</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Meiosis</topic><topic>Monosomy</topic><topic>Morphology</topic><topic>Oocytes</topic><topic>Pregnancy</topic><topic>Preimplantation Diagnosis - methods</topic><topic>Reproductive Medicine</topic><topic>Retrospective Studies</topic><topic>Sperm</topic><topic>Trisomy</topic><topic>Ultrasonic imaging</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tschare, Lena</creatorcontrib><creatorcontrib>Ennemoser, Anna</creatorcontrib><creatorcontrib>Carli, Luca</creatorcontrib><creatorcontrib>Vaccari, Enrico</creatorcontrib><creatorcontrib>Feichtinger, Michael</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of assisted reproduction and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tschare, Lena</au><au>Ennemoser, Anna</au><au>Carli, Luca</au><au>Vaccari, Enrico</au><au>Feichtinger, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impact of maternally derived meiotic aneuploidies on early embryonic development in vitro</atitle><jtitle>Journal of assisted reproduction and genetics</jtitle><stitle>J Assist Reprod Genet</stitle><addtitle>J Assist Reprod Genet</addtitle><date>2023-11-01</date><risdate>2023</risdate><volume>40</volume><issue>11</issue><spage>2715</spage><epage>2723</epage><pages>2715-2723</pages><issn>1058-0468</issn><issn>1573-7330</issn><eissn>1573-7330</eissn><abstract>Purpose
To assess early embryonic developmental potential of embryos affected by maternally inherited meiotic aneuploidies.
Methods
This observational, descriptive study includes 930 oocytes from 151 patients which were retrospectively analyzed by combining the morphological assessment with the genetic results from polar body diagnosis.
Results
Of 930 oocytes examined, 566 (60.9%) were tested aneuploid. Developmental potential until cleavage stage was not affected by trisomies or monosomies (69.6% vs. 77.1%,
p
= 0.75). However, trisomies significantly more often resulted in top quality cleavage stage embryos compared to monosomies (20% vs. 17.6%,
p
= < 0.01). Top quality blastocysts were more likely to be euploid than aneuploid (52.4% vs. 47.6%,
p
= 0.032). Additionally, significantly more aneuploid embryos resulted in developmental arrest compared to euploid embryos (15.3% vs. 6.7%,
p
= 0.003). Overall, there was no significant difference in the frequency of trisomies and monosomies in blastocyst stage embryos. (28.3% vs. 28.2%;
p
= 0.81). In contrast to earlier developmental stages, distribution of trisomies and monosomies did not differ in top quality blastocysts (8.3% vs. 5.3%,
p
= 0.32). However, certain chromosomal abnormalities showed a higher potential to develop into a top-rated blastocyst. These included monosomies 2, 5, 8, 10, 16, 17, 20, 21, and 22 and trisomies 2, 4, 5, 8, 9, 10, 11, 12, 13, 16, 17, 18 and 20.
Conclusion
Meiotically induced maternal aneuploidies have different effects on early embryonic development. While no difference in developmental potential between monosomies and trisomies could be observed in blastocysts, cleavage stage quality was significantly affected by chromosomal aneuploidies.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>37632639</pmid><doi>10.1007/s10815-023-02922-9</doi><tpages>9</tpages><orcidid>https://orcid.org/0009-0000-3644-8286</orcidid><orcidid>https://orcid.org/0000-0001-6453-9281</orcidid></addata></record> |
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| ispartof | Journal of assisted reproduction and genetics, 2023-11, Vol.40 (11), p.2715-2723 |
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| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; SpringerLink Journals - AutoHoldings |
| subjects | Aneuploidy Biopsy Blastocyst Blastocysts Chromosome aberrations Developmental stages Embryogenesis Embryology Embryonic Development - genetics Embryos Female Fertility Follicles Genetic testing Genetics Gynecology Human Genetics Humans Infertility Medicine Medicine & Public Health Meiosis Monosomy Morphology Oocytes Pregnancy Preimplantation Diagnosis - methods Reproductive Medicine Retrospective Studies Sperm Trisomy Ultrasonic imaging |
| title | Impact of maternally derived meiotic aneuploidies on early embryonic development in vitro |
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