Complete genome sequence analysis of multi-drug resistant Klebsiella pneumoniae THK strain isolated from aquaculture farm in Kerala, India
The emergence of microorganisms with resistance toward antibiotics has been widely recognized as a growing hazard to public health. Here, we report the whole genome sequence of Klebsiella pneumoniae strain THK , which was isolated from an aquaculture farm in Kerala, India. Standard disc diffusion an...
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| Veröffentlicht in: | 3 Biotech 2023-12, Vol.13 (12), p.394-394, Article 394 |
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| creator | Girijan, Sneha Kalasseril Pillai, Devika Devika, Raj |
| description | The emergence of microorganisms with resistance toward antibiotics has been widely recognized as a growing hazard to public health. Here, we report the whole genome sequence of
Klebsiella pneumoniae
strain THK
,
which was isolated from an aquaculture farm in Kerala, India. Standard disc diffusion and strip methods were used for antibiotic susceptibility testing and minimum inhibitory concentration detection. The isolate showed high level of resistance against beta-lactam, aminoglycosides, fluoroquinolones, macrolides, tetracyclines, chloramphenicol, sulfonamides, and cephalosporin class of antibiotics, but were sensitive to carbapenem and polymyxins. The whole genome sequence analysis revealed that the final assembly of THK strain consisted of 30 contigs of 5, 199,186 bp including four plasmid sequences plasmid_690 (1 contig), plasmid_710 (9 contigs), plasmid_1222 (6 contigs), and plasmid_1528 (1 contig). Sequence analysis revealed that the isolate has a number of genes encoding for antibiotic resistance and virulence. The findings indicated that antibiotics present in aquatic environments at sub-inhibitory concentrations increase the selection pressure affecting the cell function of even normal aquatic microorganisms to change the genetic expression of virulence factors or acquire resistance genes through various transfer mechanisms. This strain would be of significant interest for thorough comparative genomic study, given the known vast diversity of pathogenic and antibiotic resistance potentials. |
| doi_str_mv | 10.1007/s13205-023-03820-8 |
| format | Article |
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Klebsiella pneumoniae
strain THK
,
which was isolated from an aquaculture farm in Kerala, India. Standard disc diffusion and strip methods were used for antibiotic susceptibility testing and minimum inhibitory concentration detection. The isolate showed high level of resistance against beta-lactam, aminoglycosides, fluoroquinolones, macrolides, tetracyclines, chloramphenicol, sulfonamides, and cephalosporin class of antibiotics, but were sensitive to carbapenem and polymyxins. The whole genome sequence analysis revealed that the final assembly of THK strain consisted of 30 contigs of 5, 199,186 bp including four plasmid sequences plasmid_690 (1 contig), plasmid_710 (9 contigs), plasmid_1222 (6 contigs), and plasmid_1528 (1 contig). Sequence analysis revealed that the isolate has a number of genes encoding for antibiotic resistance and virulence. The findings indicated that antibiotics present in aquatic environments at sub-inhibitory concentrations increase the selection pressure affecting the cell function of even normal aquatic microorganisms to change the genetic expression of virulence factors or acquire resistance genes through various transfer mechanisms. This strain would be of significant interest for thorough comparative genomic study, given the known vast diversity of pathogenic and antibiotic resistance potentials.</description><identifier>ISSN: 2190-572X</identifier><identifier>ISSN: 2190-5738</identifier><identifier>EISSN: 2190-5738</identifier><identifier>DOI: 10.1007/s13205-023-03820-8</identifier><identifier>PMID: 37970451</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Agriculture ; Amides ; Aminoglycosides ; Antibiotic resistance ; Antibiotics ; Aquaculture ; Aquatic environment ; Aquatic microorganisms ; Bioinformatics ; Biomaterials ; Biotechnology ; Cancer Research ; Carbapenems ; Cephalosporins ; Chemistry ; Chemistry and Materials Science ; Chloramphenicol ; Chloromycetin ; class ; Drug resistance ; farms ; Fluoroquinolones ; Genes ; Genome Reports ; Genomes ; genomics ; India ; Klebsiella ; Klebsiella pneumoniae ; macrolides ; Microorganisms ; Minimum inhibitory concentration ; Multidrug resistance ; multiple drug resistance ; Nucleotide sequence ; nucleotide sequences ; plasmids ; Polymyxins ; Public health ; selection pressure ; Sequence analysis ; Stem Cells ; Sulfonamides ; Tetracyclines ; Virulence ; Virulence factors ; β-Lactam antibiotics</subject><ispartof>3 Biotech, 2023-12, Vol.13 (12), p.394-394, Article 394</ispartof><rights>King Abdulaziz City for Science and Technology 2023</rights><rights>King Abdulaziz City for Science and Technology 2023.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c459t-e52aa4b628cbb51e3bf9a95f953e31e52024f5010ca940e91510a78d20efeb023</cites><orcidid>0000-0003-1282-0930</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643474/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643474/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,41464,42533,51294,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37970451$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Girijan, Sneha Kalasseril</creatorcontrib><creatorcontrib>Pillai, Devika</creatorcontrib><creatorcontrib>Devika, Raj</creatorcontrib><title>Complete genome sequence analysis of multi-drug resistant Klebsiella pneumoniae THK strain isolated from aquaculture farm in Kerala, India</title><title>3 Biotech</title><addtitle>3 Biotech</addtitle><addtitle>3 Biotech</addtitle><description>The emergence of microorganisms with resistance toward antibiotics has been widely recognized as a growing hazard to public health. Here, we report the whole genome sequence of
Klebsiella pneumoniae
strain THK
,
which was isolated from an aquaculture farm in Kerala, India. Standard disc diffusion and strip methods were used for antibiotic susceptibility testing and minimum inhibitory concentration detection. The isolate showed high level of resistance against beta-lactam, aminoglycosides, fluoroquinolones, macrolides, tetracyclines, chloramphenicol, sulfonamides, and cephalosporin class of antibiotics, but were sensitive to carbapenem and polymyxins. The whole genome sequence analysis revealed that the final assembly of THK strain consisted of 30 contigs of 5, 199,186 bp including four plasmid sequences plasmid_690 (1 contig), plasmid_710 (9 contigs), plasmid_1222 (6 contigs), and plasmid_1528 (1 contig). Sequence analysis revealed that the isolate has a number of genes encoding for antibiotic resistance and virulence. The findings indicated that antibiotics present in aquatic environments at sub-inhibitory concentrations increase the selection pressure affecting the cell function of even normal aquatic microorganisms to change the genetic expression of virulence factors or acquire resistance genes through various transfer mechanisms. This strain would be of significant interest for thorough comparative genomic study, given the known vast diversity of pathogenic and antibiotic resistance potentials.</description><subject>Agriculture</subject><subject>Amides</subject><subject>Aminoglycosides</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Aquaculture</subject><subject>Aquatic environment</subject><subject>Aquatic microorganisms</subject><subject>Bioinformatics</subject><subject>Biomaterials</subject><subject>Biotechnology</subject><subject>Cancer Research</subject><subject>Carbapenems</subject><subject>Cephalosporins</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chloramphenicol</subject><subject>Chloromycetin</subject><subject>class</subject><subject>Drug resistance</subject><subject>farms</subject><subject>Fluoroquinolones</subject><subject>Genes</subject><subject>Genome Reports</subject><subject>Genomes</subject><subject>genomics</subject><subject>India</subject><subject>Klebsiella</subject><subject>Klebsiella pneumoniae</subject><subject>macrolides</subject><subject>Microorganisms</subject><subject>Minimum inhibitory concentration</subject><subject>Multidrug resistance</subject><subject>multiple drug resistance</subject><subject>Nucleotide sequence</subject><subject>nucleotide sequences</subject><subject>plasmids</subject><subject>Polymyxins</subject><subject>Public health</subject><subject>selection pressure</subject><subject>Sequence analysis</subject><subject>Stem Cells</subject><subject>Sulfonamides</subject><subject>Tetracyclines</subject><subject>Virulence</subject><subject>Virulence factors</subject><subject>β-Lactam antibiotics</subject><issn>2190-572X</issn><issn>2190-5738</issn><issn>2190-5738</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNqFkstu1DAUhiNERavSF2CBLLFh0cCxHSfxCqFRodVUYlMkdtZJcjK4cuypnSD1FXhqPEwZLgvqjS2fz7_P5S-KFxzecIDmbeJSgCpByBJkK6BsnxQngmsoVSPbp4ez-HJcnKV0C3kprjSHZ8WxbHQDleInxfdVmLaOZmIb8mEiluhuId8TQ4_uPtnEwsimxc22HOKyYZHy3Yx-ZmtHXbLkHLKtp2UK3iKxm8s1S3NE65lNweFMAxtjmBjeLdhnnSUSGzFOLBNriujwnF35weLz4mhEl-jsYT8tPn-4uFldltefPl6t3l-XfaX0XJISiFVXi7bvOsVJdqNGrUatJEmeoyCqUQGHHnUFpLnigE07CKCRutyv0-LdXne7dBMNPfmcrjPbaCeM9yagNX9HvP1qNuGb4VBXsmqqrPD6QSGG3K00m8mmftcJT2FJRnIl6yYPQD6KilZDo2pZq4y--ge9DUvMU9hRra6lUBoyJfZUH0NKkcZD4hzMzhlm7wyTKzU_nWHa_OjlnyUfnvzyQQbkHkg55DcUf__9H9kfKgHFxQ</recordid><startdate>20231201</startdate><enddate>20231201</enddate><creator>Girijan, Sneha Kalasseril</creator><creator>Pillai, Devika</creator><creator>Devika, Raj</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1282-0930</orcidid></search><sort><creationdate>20231201</creationdate><title>Complete genome sequence analysis of multi-drug resistant Klebsiella pneumoniae THK strain isolated from aquaculture farm in Kerala, India</title><author>Girijan, Sneha Kalasseril ; Pillai, Devika ; Devika, Raj</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-e52aa4b628cbb51e3bf9a95f953e31e52024f5010ca940e91510a78d20efeb023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Agriculture</topic><topic>Amides</topic><topic>Aminoglycosides</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Aquaculture</topic><topic>Aquatic environment</topic><topic>Aquatic microorganisms</topic><topic>Bioinformatics</topic><topic>Biomaterials</topic><topic>Biotechnology</topic><topic>Cancer Research</topic><topic>Carbapenems</topic><topic>Cephalosporins</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chloramphenicol</topic><topic>Chloromycetin</topic><topic>class</topic><topic>Drug resistance</topic><topic>farms</topic><topic>Fluoroquinolones</topic><topic>Genes</topic><topic>Genome Reports</topic><topic>Genomes</topic><topic>genomics</topic><topic>India</topic><topic>Klebsiella</topic><topic>Klebsiella pneumoniae</topic><topic>macrolides</topic><topic>Microorganisms</topic><topic>Minimum inhibitory concentration</topic><topic>Multidrug resistance</topic><topic>multiple drug resistance</topic><topic>Nucleotide sequence</topic><topic>nucleotide sequences</topic><topic>plasmids</topic><topic>Polymyxins</topic><topic>Public health</topic><topic>selection pressure</topic><topic>Sequence analysis</topic><topic>Stem Cells</topic><topic>Sulfonamides</topic><topic>Tetracyclines</topic><topic>Virulence</topic><topic>Virulence factors</topic><topic>β-Lactam antibiotics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Girijan, Sneha Kalasseril</creatorcontrib><creatorcontrib>Pillai, Devika</creatorcontrib><creatorcontrib>Devika, Raj</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>3 Biotech</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Girijan, Sneha Kalasseril</au><au>Pillai, Devika</au><au>Devika, Raj</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete genome sequence analysis of multi-drug resistant Klebsiella pneumoniae THK strain isolated from aquaculture farm in Kerala, India</atitle><jtitle>3 Biotech</jtitle><stitle>3 Biotech</stitle><addtitle>3 Biotech</addtitle><date>2023-12-01</date><risdate>2023</risdate><volume>13</volume><issue>12</issue><spage>394</spage><epage>394</epage><pages>394-394</pages><artnum>394</artnum><issn>2190-572X</issn><issn>2190-5738</issn><eissn>2190-5738</eissn><abstract>The emergence of microorganisms with resistance toward antibiotics has been widely recognized as a growing hazard to public health. Here, we report the whole genome sequence of
Klebsiella pneumoniae
strain THK
,
which was isolated from an aquaculture farm in Kerala, India. Standard disc diffusion and strip methods were used for antibiotic susceptibility testing and minimum inhibitory concentration detection. The isolate showed high level of resistance against beta-lactam, aminoglycosides, fluoroquinolones, macrolides, tetracyclines, chloramphenicol, sulfonamides, and cephalosporin class of antibiotics, but were sensitive to carbapenem and polymyxins. The whole genome sequence analysis revealed that the final assembly of THK strain consisted of 30 contigs of 5, 199,186 bp including four plasmid sequences plasmid_690 (1 contig), plasmid_710 (9 contigs), plasmid_1222 (6 contigs), and plasmid_1528 (1 contig). Sequence analysis revealed that the isolate has a number of genes encoding for antibiotic resistance and virulence. The findings indicated that antibiotics present in aquatic environments at sub-inhibitory concentrations increase the selection pressure affecting the cell function of even normal aquatic microorganisms to change the genetic expression of virulence factors or acquire resistance genes through various transfer mechanisms. This strain would be of significant interest for thorough comparative genomic study, given the known vast diversity of pathogenic and antibiotic resistance potentials.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>37970451</pmid><doi>10.1007/s13205-023-03820-8</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0003-1282-0930</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Springer Nature - Complete Springer Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Agriculture Amides Aminoglycosides Antibiotic resistance Antibiotics Aquaculture Aquatic environment Aquatic microorganisms Bioinformatics Biomaterials Biotechnology Cancer Research Carbapenems Cephalosporins Chemistry Chemistry and Materials Science Chloramphenicol Chloromycetin class Drug resistance farms Fluoroquinolones Genes Genome Reports Genomes genomics India Klebsiella Klebsiella pneumoniae macrolides Microorganisms Minimum inhibitory concentration Multidrug resistance multiple drug resistance Nucleotide sequence nucleotide sequences plasmids Polymyxins Public health selection pressure Sequence analysis Stem Cells Sulfonamides Tetracyclines Virulence Virulence factors β-Lactam antibiotics |
| title | Complete genome sequence analysis of multi-drug resistant Klebsiella pneumoniae THK strain isolated from aquaculture farm in Kerala, India |
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