Comparative RNA-sequencing analysis of the prostate in a mouse model of benign prostatic hyperplasia with bladder outlet obstruction
In ageing men, benign prostatic hyperplasia (BPH) is a chronic disease that leads to progressive lower urinary tract symptoms (LUTS) caused by obstruction of the bladder outlet (BOO). Patients with LUTS (such as increased frequency and urgency of urination) and complications of BOO (such as hydronep...
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| Veröffentlicht in: | Molecular and cellular biochemistry 2023-12, Vol.478 (12), p.2721-2737 |
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| creator | Tang, Xiaohu Liu, Zhiyan Ren, Jingwen Cao, Ying Xia, Shujie Sun, Zhaolin Luo, Guangheng |
| description | In ageing men, benign prostatic hyperplasia (BPH) is a chronic disease that leads to progressive lower urinary tract symptoms (LUTS) caused by obstruction of the bladder outlet (BOO). Patients with LUTS (such as increased frequency and urgency of urination) and complications of BOO (such as hydronephrosis and bladder stones) are at risk of serious health problems. BPH causes a rapidly rising burden of LUTS far exceeding that of other urological conditions. Treatment outcomes are unsatisfactory for BPH largely due to the lacking of fully understanding of the pathogenesis. Hormonal imbalances related to androgen and oestrogen can cause BPH, but the exact mechanism is still unknown, even the animal model is not fully understood. Additionally, there are no large-scale data to explain this mechanism. A BPH mouse model was established using mixed slow-release pellets of testosterone (T) and estradiol (E2), and we measured gene expression in mouse prostate tissue using RNA-seq, verified the results using qRT‒PCR, and used bioinformatics methods to analyse the differentially expressed genes (DEGs). |
| doi_str_mv | 10.1007/s11010-023-04695-2 |
| format | Article |
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Patients with LUTS (such as increased frequency and urgency of urination) and complications of BOO (such as hydronephrosis and bladder stones) are at risk of serious health problems. BPH causes a rapidly rising burden of LUTS far exceeding that of other urological conditions. Treatment outcomes are unsatisfactory for BPH largely due to the lacking of fully understanding of the pathogenesis. Hormonal imbalances related to androgen and oestrogen can cause BPH, but the exact mechanism is still unknown, even the animal model is not fully understood. Additionally, there are no large-scale data to explain this mechanism. A BPH mouse model was established using mixed slow-release pellets of testosterone (T) and estradiol (E2), and we measured gene expression in mouse prostate tissue using RNA-seq, verified the results using qRT‒PCR, and used bioinformatics methods to analyse the differentially expressed genes (DEGs).</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/s11010-023-04695-2</identifier><identifier>PMID: 36920576</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>17β-Estradiol ; Amino acids ; Animal models ; Biochemistry ; Bioinformatics ; Biomedical and Life Sciences ; Bladder ; Cancer Research ; Cardiology ; Chronic diseases ; Chronic illnesses ; Comparative analysis ; Estradiol ; Estrogens ; Gene expression ; Gene sequencing ; Genes ; Genital diseases ; Health aspects ; Health problems ; Hyperplasia ; Hypertrophy ; Kidney diseases ; Life Sciences ; Medical Biochemistry ; Mice ; Pathogenesis ; Prostate ; Ribonucleic acid ; RNA ; Sequence analysis ; Sex hormones ; Signs and symptoms ; Testosterone ; Urinary tract ; Urination</subject><ispartof>Molecular and cellular biochemistry, 2023-12, Vol.478 (12), p.2721-2737</ispartof><rights>The Author(s) 2023</rights><rights>2023. The Author(s).</rights><rights>COPYRIGHT 2023 Springer</rights><rights>The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). 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Patients with LUTS (such as increased frequency and urgency of urination) and complications of BOO (such as hydronephrosis and bladder stones) are at risk of serious health problems. BPH causes a rapidly rising burden of LUTS far exceeding that of other urological conditions. Treatment outcomes are unsatisfactory for BPH largely due to the lacking of fully understanding of the pathogenesis. Hormonal imbalances related to androgen and oestrogen can cause BPH, but the exact mechanism is still unknown, even the animal model is not fully understood. Additionally, there are no large-scale data to explain this mechanism. A BPH mouse model was established using mixed slow-release pellets of testosterone (T) and estradiol (E2), and we measured gene expression in mouse prostate tissue using RNA-seq, verified the results using qRT‒PCR, and used bioinformatics methods to analyse the differentially expressed genes (DEGs).</description><subject>17β-Estradiol</subject><subject>Amino acids</subject><subject>Animal models</subject><subject>Biochemistry</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Bladder</subject><subject>Cancer Research</subject><subject>Cardiology</subject><subject>Chronic diseases</subject><subject>Chronic illnesses</subject><subject>Comparative analysis</subject><subject>Estradiol</subject><subject>Estrogens</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Genital diseases</subject><subject>Health aspects</subject><subject>Health problems</subject><subject>Hyperplasia</subject><subject>Hypertrophy</subject><subject>Kidney diseases</subject><subject>Life Sciences</subject><subject>Medical Biochemistry</subject><subject>Mice</subject><subject>Pathogenesis</subject><subject>Prostate</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Sequence analysis</subject><subject>Sex hormones</subject><subject>Signs and symptoms</subject><subject>Testosterone</subject><subject>Urinary 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RNA-sequencing analysis of the prostate in a mouse model of benign prostatic hyperplasia with bladder outlet obstruction</title><author>Tang, Xiaohu ; Liu, Zhiyan ; Ren, Jingwen ; Cao, Ying ; Xia, Shujie ; Sun, Zhaolin ; Luo, Guangheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-69205f28d7daa7b5d5da53406d1c6b08cbf4cdc0a579146d776d7e02d56b70603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>17β-Estradiol</topic><topic>Amino acids</topic><topic>Animal models</topic><topic>Biochemistry</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Bladder</topic><topic>Cancer Research</topic><topic>Cardiology</topic><topic>Chronic diseases</topic><topic>Chronic illnesses</topic><topic>Comparative analysis</topic><topic>Estradiol</topic><topic>Estrogens</topic><topic>Gene expression</topic><topic>Gene 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benign prostatic hyperplasia (BPH) is a chronic disease that leads to progressive lower urinary tract symptoms (LUTS) caused by obstruction of the bladder outlet (BOO). Patients with LUTS (such as increased frequency and urgency of urination) and complications of BOO (such as hydronephrosis and bladder stones) are at risk of serious health problems. BPH causes a rapidly rising burden of LUTS far exceeding that of other urological conditions. Treatment outcomes are unsatisfactory for BPH largely due to the lacking of fully understanding of the pathogenesis. Hormonal imbalances related to androgen and oestrogen can cause BPH, but the exact mechanism is still unknown, even the animal model is not fully understood. Additionally, there are no large-scale data to explain this mechanism. A BPH mouse model was established using mixed slow-release pellets of testosterone (T) and estradiol (E2), and we measured gene expression in mouse prostate tissue using RNA-seq, verified the results using qRT‒PCR, and used bioinformatics methods to analyse the differentially expressed genes (DEGs).</abstract><cop>New York</cop><pub>Springer US</pub><pmid>36920576</pmid><doi>10.1007/s11010-023-04695-2</doi><tpages>17</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Molecular and cellular biochemistry, 2023-12, Vol.478 (12), p.2721-2737 |
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| subjects | 17β-Estradiol Amino acids Animal models Biochemistry Bioinformatics Biomedical and Life Sciences Bladder Cancer Research Cardiology Chronic diseases Chronic illnesses Comparative analysis Estradiol Estrogens Gene expression Gene sequencing Genes Genital diseases Health aspects Health problems Hyperplasia Hypertrophy Kidney diseases Life Sciences Medical Biochemistry Mice Pathogenesis Prostate Ribonucleic acid RNA Sequence analysis Sex hormones Signs and symptoms Testosterone Urinary tract Urination |
| title | Comparative RNA-sequencing analysis of the prostate in a mouse model of benign prostatic hyperplasia with bladder outlet obstruction |
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