Effect of D-myo-inositol 1,4,5-trisphosphate on the electrical properties of the red beet vacuole membrane
The effect of channel opening in the tonoplast by D-myo-inositol 1,4,5-trisphosphate [ins(1,4,5)P3] has been examined on red beet (Beta vulgaris) vacuoles. Patch-clamp measurements of the vacuolar potential and current were performed on vacuoles isolated in 0.1 micromolar free Ca2+ medium. With vacu...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1990-06, Vol.93 (2), p.837-840 |
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| creator | Alexandre, J. (URA CNRS, Mont-Saint-Aignan, France) Lassalles, J.P |
| description | The effect of channel opening in the tonoplast by D-myo-inositol 1,4,5-trisphosphate [ins(1,4,5)P3] has been examined on red beet (Beta vulgaris) vacuoles. Patch-clamp measurements of the vacuolar potential and current were performed on vacuoles isolated in 0.1 micromolar free Ca2+ medium. With vacuoles clamped at +30 millivolts, the ins(1,4,5)P3 induced changes in current were depending on the Ca2+ buffer strength in the external medium. The spontaneous depolarization of vacuoles in which H+-pumps were activated by 5 millimolar MgATP was increased from +6 to +18 millivolts by 1 micromolar Ins(1,4,5)P3. We have interpreted our data by assuming that even with 2 millimolar EGTA to buffer Ca2+ at 0.1 micromolar in the external medium, ins(1,4,5)P3 released enough Ca2+ from the vacuole to produce an accumulation of this ion near the tonoplast. Apart from their dependency with free Ca2+ in the cytoplasm, the electrical properties of the tonoplast could be depending on the Ins(1,4,5)P3 and Ca2+ buffer values in the cytoplasm |
| doi_str_mv | 10.1104/pp.93.2.837 |
| format | Article |
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(URA CNRS, Mont-Saint-Aignan, France) ; Lassalles, J.P</creator><creatorcontrib>Alexandre, J. (URA CNRS, Mont-Saint-Aignan, France) ; Lassalles, J.P</creatorcontrib><description>The effect of channel opening in the tonoplast by D-myo-inositol 1,4,5-trisphosphate [ins(1,4,5)P3] has been examined on red beet (Beta vulgaris) vacuoles. Patch-clamp measurements of the vacuolar potential and current were performed on vacuoles isolated in 0.1 micromolar free Ca2+ medium. With vacuoles clamped at +30 millivolts, the ins(1,4,5)P3 induced changes in current were depending on the Ca2+ buffer strength in the external medium. The spontaneous depolarization of vacuoles in which H+-pumps were activated by 5 millimolar MgATP was increased from +6 to +18 millivolts by 1 micromolar Ins(1,4,5)P3. We have interpreted our data by assuming that even with 2 millimolar EGTA to buffer Ca2+ at 0.1 micromolar in the external medium, ins(1,4,5)P3 released enough Ca2+ from the vacuole to produce an accumulation of this ion near the tonoplast. Apart from their dependency with free Ca2+ in the cytoplasm, the electrical properties of the tonoplast could be depending on the Ins(1,4,5)P3 and Ca2+ buffer values in the cytoplasm</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.93.2.837</identifier><identifier>PMID: 16667547</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Beets ; BETA VULGARIS ; Biological and medical sciences ; CALCIO ; CALCIUM ; Cell physiology ; Communications ; Cytoplasm ; Depolarization ; Electrical properties ; FOSFATOS ; Fundamental and applied biological sciences. Psychology ; INOSITOL ; inositol 1,4,5-trisphosphate ; ION ; IONES ; Membranes and Bioenergetics ; PHOSPHATE ; Pipettes ; Plant cells ; Plant physiology and development ; Plants ; Plasma membrane and permeation ; POTENCIAL ELECTRICO ; POTENTIEL ELECTRIQUE ; Tonoplast ; TRANSPORT DES SUBSTANCES NUTRITIVES ; TRANSPORTE DE NUTRIENTES ; VACUOLA ; VACUOLE ; Vacuoles</subject><ispartof>Plant physiology (Bethesda), 1990-06, Vol.93 (2), p.837-840</ispartof><rights>Copyright 1990 American Society of Plant Physiologists</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-e0b350e6fdf3ab8107f87ec41118a83b1d160863155bc9f2b7bc494ff44a27203</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4272906$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4272906$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19279964$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16667547$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alexandre, J. (URA CNRS, Mont-Saint-Aignan, France)</creatorcontrib><creatorcontrib>Lassalles, J.P</creatorcontrib><title>Effect of D-myo-inositol 1,4,5-trisphosphate on the electrical properties of the red beet vacuole membrane</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The effect of channel opening in the tonoplast by D-myo-inositol 1,4,5-trisphosphate [ins(1,4,5)P3] has been examined on red beet (Beta vulgaris) vacuoles. Patch-clamp measurements of the vacuolar potential and current were performed on vacuoles isolated in 0.1 micromolar free Ca2+ medium. With vacuoles clamped at +30 millivolts, the ins(1,4,5)P3 induced changes in current were depending on the Ca2+ buffer strength in the external medium. The spontaneous depolarization of vacuoles in which H+-pumps were activated by 5 millimolar MgATP was increased from +6 to +18 millivolts by 1 micromolar Ins(1,4,5)P3. We have interpreted our data by assuming that even with 2 millimolar EGTA to buffer Ca2+ at 0.1 micromolar in the external medium, ins(1,4,5)P3 released enough Ca2+ from the vacuole to produce an accumulation of this ion near the tonoplast. Apart from their dependency with free Ca2+ in the cytoplasm, the electrical properties of the tonoplast could be depending on the Ins(1,4,5)P3 and Ca2+ buffer values in the cytoplasm</description><subject>Beets</subject><subject>BETA VULGARIS</subject><subject>Biological and medical sciences</subject><subject>CALCIO</subject><subject>CALCIUM</subject><subject>Cell physiology</subject><subject>Communications</subject><subject>Cytoplasm</subject><subject>Depolarization</subject><subject>Electrical properties</subject><subject>FOSFATOS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>INOSITOL</subject><subject>inositol 1,4,5-trisphosphate</subject><subject>ION</subject><subject>IONES</subject><subject>Membranes and Bioenergetics</subject><subject>PHOSPHATE</subject><subject>Pipettes</subject><subject>Plant cells</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Plasma membrane and permeation</subject><subject>POTENCIAL ELECTRICO</subject><subject>POTENTIEL ELECTRIQUE</subject><subject>Tonoplast</subject><subject>TRANSPORT DES SUBSTANCES NUTRITIVES</subject><subject>TRANSPORTE DE NUTRIENTES</subject><subject>VACUOLA</subject><subject>VACUOLE</subject><subject>Vacuoles</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNp9kc1rVDEUxR-i2LG6cici2agL-8bcfLwkG0Fq_YCCC-065GVuOm94b_KaZAr9780wQ6sbFyGB88vhnnua5iXQJQAVH-d5afiSLTVXj5oFSM5aJoV-3CworW-qtTlpnuW8oZQCB_G0OYGu65QUatFsLkJAX0gM5Es73cV22MY8lDgSOBNnsi1pyPM61uMKkrglZY0Ex_olDd6NZE5xxlQGzHuLvZhwRXrEQm6d38URyYRTn9wWnzdPghszvjjep83V14vf59_by5_ffpx_vmy9BFFapD2XFLuwCtz1GqgKWqEXAKCd5j2soKO64yBl701gveq9MCIEIRxTjPLT5tPBd971E648bktyo53TMLl0Z6Mb7L_Kdljb63hrgXZMGlEN3h8NUrzZYS52GrLHcawh4i5bxbkwXCldyXf_JUFqJhWwCn44gD7FnBOG-3GA2n2Ldp6t4ZbZ2mKl3_yd4IE91laBt0fA5dpCqOv1Q37gDFPGdPskrw_cJpeY7nVR92RoV-VXBzm4aN11rdpe_TJUaBAd_wN96LdD</recordid><startdate>19900601</startdate><enddate>19900601</enddate><creator>Alexandre, J. (URA CNRS, Mont-Saint-Aignan, France)</creator><creator>Lassalles, J.P</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900601</creationdate><title>Effect of D-myo-inositol 1,4,5-trisphosphate on the electrical properties of the red beet vacuole membrane</title><author>Alexandre, J. (URA CNRS, Mont-Saint-Aignan, France) ; Lassalles, J.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-e0b350e6fdf3ab8107f87ec41118a83b1d160863155bc9f2b7bc494ff44a27203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Beets</topic><topic>BETA VULGARIS</topic><topic>Biological and medical sciences</topic><topic>CALCIO</topic><topic>CALCIUM</topic><topic>Cell physiology</topic><topic>Communications</topic><topic>Cytoplasm</topic><topic>Depolarization</topic><topic>Electrical properties</topic><topic>FOSFATOS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>INOSITOL</topic><topic>inositol 1,4,5-trisphosphate</topic><topic>ION</topic><topic>IONES</topic><topic>Membranes and Bioenergetics</topic><topic>PHOSPHATE</topic><topic>Pipettes</topic><topic>Plant cells</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Plasma membrane and permeation</topic><topic>POTENCIAL ELECTRICO</topic><topic>POTENTIEL ELECTRIQUE</topic><topic>Tonoplast</topic><topic>TRANSPORT DES SUBSTANCES NUTRITIVES</topic><topic>TRANSPORTE DE NUTRIENTES</topic><topic>VACUOLA</topic><topic>VACUOLE</topic><topic>Vacuoles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alexandre, J. (URA CNRS, Mont-Saint-Aignan, France)</creatorcontrib><creatorcontrib>Lassalles, J.P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alexandre, J. (URA CNRS, Mont-Saint-Aignan, France)</au><au>Lassalles, J.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of D-myo-inositol 1,4,5-trisphosphate on the electrical properties of the red beet vacuole membrane</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1990-06-01</date><risdate>1990</risdate><volume>93</volume><issue>2</issue><spage>837</spage><epage>840</epage><pages>837-840</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The effect of channel opening in the tonoplast by D-myo-inositol 1,4,5-trisphosphate [ins(1,4,5)P3] has been examined on red beet (Beta vulgaris) vacuoles. Patch-clamp measurements of the vacuolar potential and current were performed on vacuoles isolated in 0.1 micromolar free Ca2+ medium. With vacuoles clamped at +30 millivolts, the ins(1,4,5)P3 induced changes in current were depending on the Ca2+ buffer strength in the external medium. The spontaneous depolarization of vacuoles in which H+-pumps were activated by 5 millimolar MgATP was increased from +6 to +18 millivolts by 1 micromolar Ins(1,4,5)P3. We have interpreted our data by assuming that even with 2 millimolar EGTA to buffer Ca2+ at 0.1 micromolar in the external medium, ins(1,4,5)P3 released enough Ca2+ from the vacuole to produce an accumulation of this ion near the tonoplast. Apart from their dependency with free Ca2+ in the cytoplasm, the electrical properties of the tonoplast could be depending on the Ins(1,4,5)P3 and Ca2+ buffer values in the cytoplasm</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16667547</pmid><doi>10.1104/pp.93.2.837</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1990-06, Vol.93 (2), p.837-840 |
| issn | 0032-0889 1532-2548 |
| language | eng |
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| source | Alma/SFX Local Collection; JSTOR; EZB Electronic Journals Library |
| subjects | Beets BETA VULGARIS Biological and medical sciences CALCIO CALCIUM Cell physiology Communications Cytoplasm Depolarization Electrical properties FOSFATOS Fundamental and applied biological sciences. Psychology INOSITOL inositol 1,4,5-trisphosphate ION IONES Membranes and Bioenergetics PHOSPHATE Pipettes Plant cells Plant physiology and development Plants Plasma membrane and permeation POTENCIAL ELECTRICO POTENTIEL ELECTRIQUE Tonoplast TRANSPORT DES SUBSTANCES NUTRITIVES TRANSPORTE DE NUTRIENTES VACUOLA VACUOLE Vacuoles |
| title | Effect of D-myo-inositol 1,4,5-trisphosphate on the electrical properties of the red beet vacuole membrane |
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