Alternative oxidase of potato is an integral membrane protein synthesized de novo during aging of tuber slices
The rise in alternative respiratory capacity upon aging of potato (Solanum tuberosum) tuber slices is correlated with changes in mitochondrial membrane protein composition and a requirement for cytoplasmic protein synthesis. However, the lack of an antibody specific to the alternative oxidase has, u...
Gespeichert in:
| Veröffentlicht in: | Plant physiology (Bethesda) 1990-05, Vol.93 (1), p.312-318 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 318 |
|---|---|
| container_issue | 1 |
| container_start_page | 312 |
| container_title | Plant physiology (Bethesda) |
| container_volume | 93 |
| creator | Hiser, C. (Michigan State University, East Lansing, MI) McIntosh, L |
| description | The rise in alternative respiratory capacity upon aging of potato (Solanum tuberosum) tuber slices is correlated with changes in mitochondrial membrane protein composition and a requirement for cytoplasmic protein synthesis. However, the lack of an antibody specific to the alternative oxidase has, until recently, prevented examination of the alternative oxidase protein(s) itself. We have employed a monoclonal antibody raised against the Sauromatum guttatum alternative oxidase to investigate developmental changes in the alternative pathway of aging potato slice mitochondria and to characterize the potato alternative oxidase by one- and two-dimensional gel electrophoresis. The relative levels of a 36 kilodalton protein parallel the rise in alternative path capacity. A plausible interpretation is that this alternative oxidase protein is synthesized de novo during aging of potato slices |
| doi_str_mv | 10.1104/pp.93.1.312 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1062504</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4272818</jstor_id><sourcerecordid>4272818</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4912-4ccd66203edad5942c393d70d6d929eb3b4f4ccb6ff1b557024f898ef038d563</originalsourceid><addsrcrecordid>eNpVkc2L1TAUxYsoznN05U5EgiAu5D3z2TYbYRj8ggEXjuuQJrd9GdqkJunD8a83zz5mdJMbOD_OPdxTVc8J3hGC-ft53km2IztG6INqQwSjWyp4-7DaYFz-uG3lWfUkpRuMMWGEP67OSF3XDRd0U_mLMUP0OrsDoPDLWZ3K7NEcss4BuYS0R85nGKIe0QRTF7UHNMeQwXmUbn3eQ3K_wSILyIdDQHaJzg9ID8e3WOWlg4jS6Aykp9WjXo8Jnp3meXX96eP15Zft1bfPXy8vrraGS0K33Bhb1xQzsNoKyalhktkG29pKKqFjHe8L09V9TzohGkx538oWesxaK2p2Xn1Ybeelm8Aa8LnEV3N0k463Kmin_le826shHBTBNRWYF4PXq0FI2alkXAazN8F7MFkJKZr2L_T2tCWGnwukrCaXDIxjuVBYkmoY45KJ5ki-W0kTQ0oR-rsoBKtjiWqelWSKqFJioV_9m_6ePbVWgDcnQCejx75UYly65yTjuCHHM7xcuZuUQ7zTOW1oS9oiv1jlXgelh1gsfnyXmGNcS_YHCtO4aA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733493574</pqid></control><display><type>article</type><title>Alternative oxidase of potato is an integral membrane protein synthesized de novo during aging of tuber slices</title><source>JSTOR Archive Collection A-Z Listing</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Hiser, C. (Michigan State University, East Lansing, MI) ; McIntosh, L</creator><creatorcontrib>Hiser, C. (Michigan State University, East Lansing, MI) ; McIntosh, L</creatorcontrib><description>The rise in alternative respiratory capacity upon aging of potato (Solanum tuberosum) tuber slices is correlated with changes in mitochondrial membrane protein composition and a requirement for cytoplasmic protein synthesis. However, the lack of an antibody specific to the alternative oxidase has, until recently, prevented examination of the alternative oxidase protein(s) itself. We have employed a monoclonal antibody raised against the Sauromatum guttatum alternative oxidase to investigate developmental changes in the alternative pathway of aging potato slice mitochondria and to characterize the potato alternative oxidase by one- and two-dimensional gel electrophoresis. The relative levels of a 36 kilodalton protein parallel the rise in alternative path capacity. A plausible interpretation is that this alternative oxidase protein is synthesized de novo during aging of potato slices</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.93.1.312</identifier><identifier>PMID: 16667452</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>551000 - Physiological Systems ; ANTIBODIES ; BASIC BIOLOGICAL SCIENCES ; BIOASSAY ; Biological and medical sciences ; BIOLOGICAL PATHWAYS ; CELL CONSTITUENTS ; COMPOSICION ; COMPOSITION ; Electrophoresis ; ENVEJECIMIENTO ; ENZYMES ; Fundamental and applied biological sciences. Psychology ; Gels ; IMMUNOASSAY ; MAGNOLIOPHYTA ; MAGNOLIOPSIDA ; MEMBRANAS CELULARES ; MEMBRANE CELLULAIRE ; Membrane proteins ; Metabolism ; MITOCHONDRIA ; Mitochondrial membranes ; MITOCHONDRIE ; MITOCONDRIA ; Molecular Biology and Gene Regulation ; MONOCLONAL ANTIBODIES ; ORGANOIDS ; Oxidases ; OXIDOREDUCTASES ; OXIDORREDUCTASAS ; OXYDOREDUCTASE ; Oxygen ; PHYSIOLOGY ; Plant physiology and development ; PLANTS ; PROTEINAS ; PROTEINE ; RESPIRACION ; RESPIRATION ; SINTESIS DE PROTEINAS ; SOLANUM ; SOLANUM TUBEROSUM ; SYNTHESE PROTEIQUE ; TUBERCULE ; TUBERCULO ; Tubers ; VIEILLISSEMENT</subject><ispartof>Plant physiology (Bethesda), 1990-05, Vol.93 (1), p.312-318</ispartof><rights>Copyright 1990 American Society of Plant Physiologists</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4912-4ccd66203edad5942c393d70d6d929eb3b4f4ccb6ff1b557024f898ef038d563</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4272818$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4272818$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19340716$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16667452$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5957804$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Hiser, C. (Michigan State University, East Lansing, MI)</creatorcontrib><creatorcontrib>McIntosh, L</creatorcontrib><title>Alternative oxidase of potato is an integral membrane protein synthesized de novo during aging of tuber slices</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The rise in alternative respiratory capacity upon aging of potato (Solanum tuberosum) tuber slices is correlated with changes in mitochondrial membrane protein composition and a requirement for cytoplasmic protein synthesis. However, the lack of an antibody specific to the alternative oxidase has, until recently, prevented examination of the alternative oxidase protein(s) itself. We have employed a monoclonal antibody raised against the Sauromatum guttatum alternative oxidase to investigate developmental changes in the alternative pathway of aging potato slice mitochondria and to characterize the potato alternative oxidase by one- and two-dimensional gel electrophoresis. The relative levels of a 36 kilodalton protein parallel the rise in alternative path capacity. A plausible interpretation is that this alternative oxidase protein is synthesized de novo during aging of potato slices</description><subject>551000 - Physiological Systems</subject><subject>ANTIBODIES</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BIOASSAY</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL PATHWAYS</subject><subject>CELL CONSTITUENTS</subject><subject>COMPOSICION</subject><subject>COMPOSITION</subject><subject>Electrophoresis</subject><subject>ENVEJECIMIENTO</subject><subject>ENZYMES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>IMMUNOASSAY</subject><subject>MAGNOLIOPHYTA</subject><subject>MAGNOLIOPSIDA</subject><subject>MEMBRANAS CELULARES</subject><subject>MEMBRANE CELLULAIRE</subject><subject>Membrane proteins</subject><subject>Metabolism</subject><subject>MITOCHONDRIA</subject><subject>Mitochondrial membranes</subject><subject>MITOCHONDRIE</subject><subject>MITOCONDRIA</subject><subject>Molecular Biology and Gene Regulation</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>ORGANOIDS</subject><subject>Oxidases</subject><subject>OXIDOREDUCTASES</subject><subject>OXIDORREDUCTASAS</subject><subject>OXYDOREDUCTASE</subject><subject>Oxygen</subject><subject>PHYSIOLOGY</subject><subject>Plant physiology and development</subject><subject>PLANTS</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>RESPIRACION</subject><subject>RESPIRATION</subject><subject>SINTESIS DE PROTEINAS</subject><subject>SOLANUM</subject><subject>SOLANUM TUBEROSUM</subject><subject>SYNTHESE PROTEIQUE</subject><subject>TUBERCULE</subject><subject>TUBERCULO</subject><subject>Tubers</subject><subject>VIEILLISSEMENT</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNpVkc2L1TAUxYsoznN05U5EgiAu5D3z2TYbYRj8ggEXjuuQJrd9GdqkJunD8a83zz5mdJMbOD_OPdxTVc8J3hGC-ft53km2IztG6INqQwSjWyp4-7DaYFz-uG3lWfUkpRuMMWGEP67OSF3XDRd0U_mLMUP0OrsDoPDLWZ3K7NEcss4BuYS0R85nGKIe0QRTF7UHNMeQwXmUbn3eQ3K_wSILyIdDQHaJzg9ID8e3WOWlg4jS6Aykp9WjXo8Jnp3meXX96eP15Zft1bfPXy8vrraGS0K33Bhb1xQzsNoKyalhktkG29pKKqFjHe8L09V9TzohGkx538oWesxaK2p2Xn1Ybeelm8Aa8LnEV3N0k463Kmin_le826shHBTBNRWYF4PXq0FI2alkXAazN8F7MFkJKZr2L_T2tCWGnwukrCaXDIxjuVBYkmoY45KJ5ki-W0kTQ0oR-rsoBKtjiWqelWSKqFJioV_9m_6ePbVWgDcnQCejx75UYly65yTjuCHHM7xcuZuUQ7zTOW1oS9oiv1jlXgelh1gsfnyXmGNcS_YHCtO4aA</recordid><startdate>19900501</startdate><enddate>19900501</enddate><creator>Hiser, C. (Michigan State University, East Lansing, MI)</creator><creator>McIntosh, L</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19900501</creationdate><title>Alternative oxidase of potato is an integral membrane protein synthesized de novo during aging of tuber slices</title><author>Hiser, C. (Michigan State University, East Lansing, MI) ; McIntosh, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4912-4ccd66203edad5942c393d70d6d929eb3b4f4ccb6ff1b557024f898ef038d563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>551000 - Physiological Systems</topic><topic>ANTIBODIES</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BIOASSAY</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL PATHWAYS</topic><topic>CELL CONSTITUENTS</topic><topic>COMPOSICION</topic><topic>COMPOSITION</topic><topic>Electrophoresis</topic><topic>ENVEJECIMIENTO</topic><topic>ENZYMES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>IMMUNOASSAY</topic><topic>MAGNOLIOPHYTA</topic><topic>MAGNOLIOPSIDA</topic><topic>MEMBRANAS CELULARES</topic><topic>MEMBRANE CELLULAIRE</topic><topic>Membrane proteins</topic><topic>Metabolism</topic><topic>MITOCHONDRIA</topic><topic>Mitochondrial membranes</topic><topic>MITOCHONDRIE</topic><topic>MITOCONDRIA</topic><topic>Molecular Biology and Gene Regulation</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>ORGANOIDS</topic><topic>Oxidases</topic><topic>OXIDOREDUCTASES</topic><topic>OXIDORREDUCTASAS</topic><topic>OXYDOREDUCTASE</topic><topic>Oxygen</topic><topic>PHYSIOLOGY</topic><topic>Plant physiology and development</topic><topic>PLANTS</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>RESPIRACION</topic><topic>RESPIRATION</topic><topic>SINTESIS DE PROTEINAS</topic><topic>SOLANUM</topic><topic>SOLANUM TUBEROSUM</topic><topic>SYNTHESE PROTEIQUE</topic><topic>TUBERCULE</topic><topic>TUBERCULO</topic><topic>Tubers</topic><topic>VIEILLISSEMENT</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hiser, C. (Michigan State University, East Lansing, MI)</creatorcontrib><creatorcontrib>McIntosh, L</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hiser, C. (Michigan State University, East Lansing, MI)</au><au>McIntosh, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alternative oxidase of potato is an integral membrane protein synthesized de novo during aging of tuber slices</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1990-05-01</date><risdate>1990</risdate><volume>93</volume><issue>1</issue><spage>312</spage><epage>318</epage><pages>312-318</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The rise in alternative respiratory capacity upon aging of potato (Solanum tuberosum) tuber slices is correlated with changes in mitochondrial membrane protein composition and a requirement for cytoplasmic protein synthesis. However, the lack of an antibody specific to the alternative oxidase has, until recently, prevented examination of the alternative oxidase protein(s) itself. We have employed a monoclonal antibody raised against the Sauromatum guttatum alternative oxidase to investigate developmental changes in the alternative pathway of aging potato slice mitochondria and to characterize the potato alternative oxidase by one- and two-dimensional gel electrophoresis. The relative levels of a 36 kilodalton protein parallel the rise in alternative path capacity. A plausible interpretation is that this alternative oxidase protein is synthesized de novo during aging of potato slices</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16667452</pmid><doi>10.1104/pp.93.1.312</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1990-05, Vol.93 (1), p.312-318 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1062504 |
| source | JSTOR Archive Collection A-Z Listing; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | 551000 - Physiological Systems ANTIBODIES BASIC BIOLOGICAL SCIENCES BIOASSAY Biological and medical sciences BIOLOGICAL PATHWAYS CELL CONSTITUENTS COMPOSICION COMPOSITION Electrophoresis ENVEJECIMIENTO ENZYMES Fundamental and applied biological sciences. Psychology Gels IMMUNOASSAY MAGNOLIOPHYTA MAGNOLIOPSIDA MEMBRANAS CELULARES MEMBRANE CELLULAIRE Membrane proteins Metabolism MITOCHONDRIA Mitochondrial membranes MITOCHONDRIE MITOCONDRIA Molecular Biology and Gene Regulation MONOCLONAL ANTIBODIES ORGANOIDS Oxidases OXIDOREDUCTASES OXIDORREDUCTASAS OXYDOREDUCTASE Oxygen PHYSIOLOGY Plant physiology and development PLANTS PROTEINAS PROTEINE RESPIRACION RESPIRATION SINTESIS DE PROTEINAS SOLANUM SOLANUM TUBEROSUM SYNTHESE PROTEIQUE TUBERCULE TUBERCULO Tubers VIEILLISSEMENT |
| title | Alternative oxidase of potato is an integral membrane protein synthesized de novo during aging of tuber slices |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T09%3A40%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Alternative%20oxidase%20of%20potato%20is%20an%20integral%20membrane%20protein%20synthesized%20de%20novo%20during%20aging%20of%20tuber%20slices&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Hiser,%20C.%20(Michigan%20State%20University,%20East%20Lansing,%20MI)&rft.date=1990-05-01&rft.volume=93&rft.issue=1&rft.spage=312&rft.epage=318&rft.pages=312-318&rft.issn=0032-0889&rft.eissn=1532-2548&rft.coden=PPHYA5&rft_id=info:doi/10.1104/pp.93.1.312&rft_dat=%3Cjstor_pubme%3E4272818%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733493574&rft_id=info:pmid/16667452&rft_jstor_id=4272818&rfr_iscdi=true |