Clove volatile oil-loaded nanoemulsion reduces the anxious-like behavior in adult zebrafish
Background Clove volatile oil (CVO) and its major compound, eugenol (EUG), have anxiolytic effects, but their clinical use has been impaired due to their low bioavailability. Thus, their encapsulation in nanosystems can be an alternative to overcome these limitations. Objectives This work aims to pr...
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| Veröffentlicht in: | Daru 2023-08, Vol.31 (2), p.183-192 |
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| creator | da Silva Campelo, Matheus Câmara Neto, João Francisco de Souza, Álamo Lourenço Ferreira, Maria Kueirislene Amâncio dos Santos, Hélcio Silva Gramosa, Nilce Viana de Aguiar Soares, Sandra Ricardo, Nágila Maria Pontes Silva de Menezes, Jane Eire Silva Alencar Ribeiro, Maria Elenir Nobre Pinho |
| description | Background
Clove volatile oil (CVO) and its major compound, eugenol (EUG), have anxiolytic effects, but their clinical use has been impaired due to their low bioavailability. Thus, their encapsulation in nanosystems can be an alternative to overcome these limitations.
Objectives
This work aims to prepare, characterize and study the anxiolytic potential of CVO loaded-nanoemulsions (CVO-NE) against anxious-like behavior in adult zebrafish (
Danio rerio
).
Methods
The CVO-NE was prepared using
Agaricus blazei
Murill polysaccharides as stabilizing agent. The drug-excipient interactions were performed, as well as colloidal characterization of CVO-NE and empty nanoemulsion (B-NE). The acute toxicity and potential anxiolytic activity of CVO, EUG, CVO-NE and B-NE against adult zebrafish models were determined.
Results
CVO, EUG, CVO-NE and B-NE presented low acute toxicity, reduced the locomotor activity and anxious-like behavior of the zebrafish at 4 – 20 mg kg
–1
. CVO-NE reduced the anxious-like behavior of adult zebrafish without affecting their locomotor activity. In addition, it was demonstrated that anxiolytic activity of CVO, EUG and CVO-NE is linked to the involvement of GABAergic pathway.
Conclusion
Therefore, this study demonstrates the anxiolytic effect of CVO, in addition to providing a new nanoformulation for its administration.
Graphical Abstract |
| doi_str_mv | 10.1007/s40199-023-00473-z |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10624781</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A762646560</galeid><sourcerecordid>A762646560</sourcerecordid><originalsourceid>FETCH-LOGICAL-c573t-ba3a43f1a9b6c277a85146bb596c1ea423c305278c47d887818738648e2bcbf63</originalsourceid><addsrcrecordid>eNp9kkuLFDEUhQtRnLH1D7iQgCBuasyrktRKhsYXDLjRlYuQSt3qyphKxqSqGfvXm7HHmW4RySKB-90T7rmnqp4TfEYwlm8yx6Rta0xZjTGXrN49qE4pxqqmlJGHB--T6knOlxgzxQV9XJ0wKVhLuDytvq193ALaRm9m5wFF52sfTQ89CiZEmBafXQwoQb9YyGgeAZlw7eKSa---A-pgNFsXE3IBmX7xM9pBl8zg8vi0ejQYn-HZ7b2qvr5_92X9sb74_OHT-vyito1kc90ZZjgbiGk7YamURjWEi65rWmEJGE6ZZbihUlkue6WkIkoyJbgC2tluEGxVvd3rXi3dBL2FMCfj9VVyk0k_dTROH1eCG_UmbjXBgvKiVxRe3yqk-GOBPOvJZQvemwBlUk1VozhueDF5Vb38C72MSwplvkKpprjKKb-nNsaDdmGI5WN7I6rPpaCCi0bgQp39gyqnh8nZGGAoKzlueHXQMILx85ijX-ayonwM0j1oU8w5wXDnBsH6Jjx6Hx5dwqN_h0fvStOLQx_vWv6kpQBsD-RSChtI97P_R_YXWN_Orw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2885914424</pqid></control><display><type>article</type><title>Clove volatile oil-loaded nanoemulsion reduces the anxious-like behavior in adult zebrafish</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>da Silva Campelo, Matheus ; Câmara Neto, João Francisco ; de Souza, Álamo Lourenço ; Ferreira, Maria Kueirislene Amâncio ; dos Santos, Hélcio Silva ; Gramosa, Nilce Viana ; de Aguiar Soares, Sandra ; Ricardo, Nágila Maria Pontes Silva ; de Menezes, Jane Eire Silva Alencar ; Ribeiro, Maria Elenir Nobre Pinho</creator><creatorcontrib>da Silva Campelo, Matheus ; Câmara Neto, João Francisco ; de Souza, Álamo Lourenço ; Ferreira, Maria Kueirislene Amâncio ; dos Santos, Hélcio Silva ; Gramosa, Nilce Viana ; de Aguiar Soares, Sandra ; Ricardo, Nágila Maria Pontes Silva ; de Menezes, Jane Eire Silva Alencar ; Ribeiro, Maria Elenir Nobre Pinho</creatorcontrib><description>Background
Clove volatile oil (CVO) and its major compound, eugenol (EUG), have anxiolytic effects, but their clinical use has been impaired due to their low bioavailability. Thus, their encapsulation in nanosystems can be an alternative to overcome these limitations.
Objectives
This work aims to prepare, characterize and study the anxiolytic potential of CVO loaded-nanoemulsions (CVO-NE) against anxious-like behavior in adult zebrafish (
Danio rerio
).
Methods
The CVO-NE was prepared using
Agaricus blazei
Murill polysaccharides as stabilizing agent. The drug-excipient interactions were performed, as well as colloidal characterization of CVO-NE and empty nanoemulsion (B-NE). The acute toxicity and potential anxiolytic activity of CVO, EUG, CVO-NE and B-NE against adult zebrafish models were determined.
Results
CVO, EUG, CVO-NE and B-NE presented low acute toxicity, reduced the locomotor activity and anxious-like behavior of the zebrafish at 4 – 20 mg kg
–1
. CVO-NE reduced the anxious-like behavior of adult zebrafish without affecting their locomotor activity. In addition, it was demonstrated that anxiolytic activity of CVO, EUG and CVO-NE is linked to the involvement of GABAergic pathway.
Conclusion
Therefore, this study demonstrates the anxiolytic effect of CVO, in addition to providing a new nanoformulation for its administration.
Graphical Abstract</description><identifier>ISSN: 2008-2231</identifier><identifier>ISSN: 1560-8115</identifier><identifier>EISSN: 2008-2231</identifier><identifier>DOI: 10.1007/s40199-023-00473-z</identifier><identifier>PMID: 37639147</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Analysis ; Animals ; Anti-Anxiety Agents - metabolism ; Anti-Anxiety Agents - pharmacology ; Antianxiety agents ; Biomedical and Life Sciences ; Biomedicine ; Clove Oil - metabolism ; Clove Oil - pharmacology ; Eugenol - metabolism ; Eugenol - pharmacology ; Flumazenil ; GABA ; Medicinal Chemistry ; Nanoemulsions ; Oils, Volatile - pharmacology ; Pharmaceutical Sciences/Technology ; Pharmacology/Toxicology ; Polysaccharides ; Research Article ; Syzygium - metabolism ; Toxicity ; Zebrafish</subject><ispartof>Daru, 2023-08, Vol.31 (2), p.183-192</ispartof><rights>The Author(s), under exclusive licence to Tehran University of Medical Sciences 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2023. The Author(s), under exclusive licence to Tehran University of Medical Sciences.</rights><rights>COPYRIGHT 2023 BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-ba3a43f1a9b6c277a85146bb596c1ea423c305278c47d887818738648e2bcbf63</citedby><cites>FETCH-LOGICAL-c573t-ba3a43f1a9b6c277a85146bb596c1ea423c305278c47d887818738648e2bcbf63</cites><orcidid>0000-0003-0286-1974 ; 0000-0003-4790-427X ; 0000-0001-9632-5464 ; 0000-0002-2678-8188 ; 0000-0002-4270-8109 ; 0000-0002-8964-6398 ; 0000-0001-6896-7179 ; 0000-0001-5527-164X ; 0000-0003-0516-3143 ; 0000-0003-1849-5403</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624781/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624781/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,41469,42538,51300,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37639147$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Silva Campelo, Matheus</creatorcontrib><creatorcontrib>Câmara Neto, João Francisco</creatorcontrib><creatorcontrib>de Souza, Álamo Lourenço</creatorcontrib><creatorcontrib>Ferreira, Maria Kueirislene Amâncio</creatorcontrib><creatorcontrib>dos Santos, Hélcio Silva</creatorcontrib><creatorcontrib>Gramosa, Nilce Viana</creatorcontrib><creatorcontrib>de Aguiar Soares, Sandra</creatorcontrib><creatorcontrib>Ricardo, Nágila Maria Pontes Silva</creatorcontrib><creatorcontrib>de Menezes, Jane Eire Silva Alencar</creatorcontrib><creatorcontrib>Ribeiro, Maria Elenir Nobre Pinho</creatorcontrib><title>Clove volatile oil-loaded nanoemulsion reduces the anxious-like behavior in adult zebrafish</title><title>Daru</title><addtitle>DARU J Pharm Sci</addtitle><addtitle>Daru</addtitle><description>Background
Clove volatile oil (CVO) and its major compound, eugenol (EUG), have anxiolytic effects, but their clinical use has been impaired due to their low bioavailability. Thus, their encapsulation in nanosystems can be an alternative to overcome these limitations.
Objectives
This work aims to prepare, characterize and study the anxiolytic potential of CVO loaded-nanoemulsions (CVO-NE) against anxious-like behavior in adult zebrafish (
Danio rerio
).
Methods
The CVO-NE was prepared using
Agaricus blazei
Murill polysaccharides as stabilizing agent. The drug-excipient interactions were performed, as well as colloidal characterization of CVO-NE and empty nanoemulsion (B-NE). The acute toxicity and potential anxiolytic activity of CVO, EUG, CVO-NE and B-NE against adult zebrafish models were determined.
Results
CVO, EUG, CVO-NE and B-NE presented low acute toxicity, reduced the locomotor activity and anxious-like behavior of the zebrafish at 4 – 20 mg kg
–1
. CVO-NE reduced the anxious-like behavior of adult zebrafish without affecting their locomotor activity. In addition, it was demonstrated that anxiolytic activity of CVO, EUG and CVO-NE is linked to the involvement of GABAergic pathway.
Conclusion
Therefore, this study demonstrates the anxiolytic effect of CVO, in addition to providing a new nanoformulation for its administration.
Graphical Abstract</description><subject>Analysis</subject><subject>Animals</subject><subject>Anti-Anxiety Agents - metabolism</subject><subject>Anti-Anxiety Agents - pharmacology</subject><subject>Antianxiety agents</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Clove Oil - metabolism</subject><subject>Clove Oil - pharmacology</subject><subject>Eugenol - metabolism</subject><subject>Eugenol - pharmacology</subject><subject>Flumazenil</subject><subject>GABA</subject><subject>Medicinal Chemistry</subject><subject>Nanoemulsions</subject><subject>Oils, Volatile - pharmacology</subject><subject>Pharmaceutical Sciences/Technology</subject><subject>Pharmacology/Toxicology</subject><subject>Polysaccharides</subject><subject>Research Article</subject><subject>Syzygium - metabolism</subject><subject>Toxicity</subject><subject>Zebrafish</subject><issn>2008-2231</issn><issn>1560-8115</issn><issn>2008-2231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kkuLFDEUhQtRnLH1D7iQgCBuasyrktRKhsYXDLjRlYuQSt3qyphKxqSqGfvXm7HHmW4RySKB-90T7rmnqp4TfEYwlm8yx6Rta0xZjTGXrN49qE4pxqqmlJGHB--T6knOlxgzxQV9XJ0wKVhLuDytvq193ALaRm9m5wFF52sfTQ89CiZEmBafXQwoQb9YyGgeAZlw7eKSa---A-pgNFsXE3IBmX7xM9pBl8zg8vi0ejQYn-HZ7b2qvr5_92X9sb74_OHT-vyito1kc90ZZjgbiGk7YamURjWEi65rWmEJGE6ZZbihUlkue6WkIkoyJbgC2tluEGxVvd3rXi3dBL2FMCfj9VVyk0k_dTROH1eCG_UmbjXBgvKiVxRe3yqk-GOBPOvJZQvemwBlUk1VozhueDF5Vb38C72MSwplvkKpprjKKb-nNsaDdmGI5WN7I6rPpaCCi0bgQp39gyqnh8nZGGAoKzlueHXQMILx85ijX-ayonwM0j1oU8w5wXDnBsH6Jjx6Hx5dwqN_h0fvStOLQx_vWv6kpQBsD-RSChtI97P_R_YXWN_Orw</recordid><startdate>20230828</startdate><enddate>20230828</enddate><creator>da Silva Campelo, Matheus</creator><creator>Câmara Neto, João Francisco</creator><creator>de Souza, Álamo Lourenço</creator><creator>Ferreira, Maria Kueirislene Amâncio</creator><creator>dos Santos, Hélcio Silva</creator><creator>Gramosa, Nilce Viana</creator><creator>de Aguiar Soares, Sandra</creator><creator>Ricardo, Nágila Maria Pontes Silva</creator><creator>de Menezes, Jane Eire Silva Alencar</creator><creator>Ribeiro, Maria Elenir Nobre Pinho</creator><general>Springer International Publishing</general><general>BioMed Central Ltd</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0286-1974</orcidid><orcidid>https://orcid.org/0000-0003-4790-427X</orcidid><orcidid>https://orcid.org/0000-0001-9632-5464</orcidid><orcidid>https://orcid.org/0000-0002-2678-8188</orcidid><orcidid>https://orcid.org/0000-0002-4270-8109</orcidid><orcidid>https://orcid.org/0000-0002-8964-6398</orcidid><orcidid>https://orcid.org/0000-0001-6896-7179</orcidid><orcidid>https://orcid.org/0000-0001-5527-164X</orcidid><orcidid>https://orcid.org/0000-0003-0516-3143</orcidid><orcidid>https://orcid.org/0000-0003-1849-5403</orcidid></search><sort><creationdate>20230828</creationdate><title>Clove volatile oil-loaded nanoemulsion reduces the anxious-like behavior in adult zebrafish</title><author>da Silva Campelo, Matheus ; Câmara Neto, João Francisco ; de Souza, Álamo Lourenço ; Ferreira, Maria Kueirislene Amâncio ; dos Santos, Hélcio Silva ; Gramosa, Nilce Viana ; de Aguiar Soares, Sandra ; Ricardo, Nágila Maria Pontes Silva ; de Menezes, Jane Eire Silva Alencar ; Ribeiro, Maria Elenir Nobre Pinho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c573t-ba3a43f1a9b6c277a85146bb596c1ea423c305278c47d887818738648e2bcbf63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Anti-Anxiety Agents - metabolism</topic><topic>Anti-Anxiety Agents - pharmacology</topic><topic>Antianxiety agents</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Clove Oil - metabolism</topic><topic>Clove Oil - pharmacology</topic><topic>Eugenol - metabolism</topic><topic>Eugenol - pharmacology</topic><topic>Flumazenil</topic><topic>GABA</topic><topic>Medicinal Chemistry</topic><topic>Nanoemulsions</topic><topic>Oils, Volatile - pharmacology</topic><topic>Pharmaceutical Sciences/Technology</topic><topic>Pharmacology/Toxicology</topic><topic>Polysaccharides</topic><topic>Research Article</topic><topic>Syzygium - metabolism</topic><topic>Toxicity</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>da Silva Campelo, Matheus</creatorcontrib><creatorcontrib>Câmara Neto, João Francisco</creatorcontrib><creatorcontrib>de Souza, Álamo Lourenço</creatorcontrib><creatorcontrib>Ferreira, Maria Kueirislene Amâncio</creatorcontrib><creatorcontrib>dos Santos, Hélcio Silva</creatorcontrib><creatorcontrib>Gramosa, Nilce Viana</creatorcontrib><creatorcontrib>de Aguiar Soares, Sandra</creatorcontrib><creatorcontrib>Ricardo, Nágila Maria Pontes Silva</creatorcontrib><creatorcontrib>de Menezes, Jane Eire Silva Alencar</creatorcontrib><creatorcontrib>Ribeiro, Maria Elenir Nobre Pinho</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Daru</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Silva Campelo, Matheus</au><au>Câmara Neto, João Francisco</au><au>de Souza, Álamo Lourenço</au><au>Ferreira, Maria Kueirislene Amâncio</au><au>dos Santos, Hélcio Silva</au><au>Gramosa, Nilce Viana</au><au>de Aguiar Soares, Sandra</au><au>Ricardo, Nágila Maria Pontes Silva</au><au>de Menezes, Jane Eire Silva Alencar</au><au>Ribeiro, Maria Elenir Nobre Pinho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clove volatile oil-loaded nanoemulsion reduces the anxious-like behavior in adult zebrafish</atitle><jtitle>Daru</jtitle><stitle>DARU J Pharm Sci</stitle><addtitle>Daru</addtitle><date>2023-08-28</date><risdate>2023</risdate><volume>31</volume><issue>2</issue><spage>183</spage><epage>192</epage><pages>183-192</pages><issn>2008-2231</issn><issn>1560-8115</issn><eissn>2008-2231</eissn><abstract>Background
Clove volatile oil (CVO) and its major compound, eugenol (EUG), have anxiolytic effects, but their clinical use has been impaired due to their low bioavailability. Thus, their encapsulation in nanosystems can be an alternative to overcome these limitations.
Objectives
This work aims to prepare, characterize and study the anxiolytic potential of CVO loaded-nanoemulsions (CVO-NE) against anxious-like behavior in adult zebrafish (
Danio rerio
).
Methods
The CVO-NE was prepared using
Agaricus blazei
Murill polysaccharides as stabilizing agent. The drug-excipient interactions were performed, as well as colloidal characterization of CVO-NE and empty nanoemulsion (B-NE). The acute toxicity and potential anxiolytic activity of CVO, EUG, CVO-NE and B-NE against adult zebrafish models were determined.
Results
CVO, EUG, CVO-NE and B-NE presented low acute toxicity, reduced the locomotor activity and anxious-like behavior of the zebrafish at 4 – 20 mg kg
–1
. CVO-NE reduced the anxious-like behavior of adult zebrafish without affecting their locomotor activity. In addition, it was demonstrated that anxiolytic activity of CVO, EUG and CVO-NE is linked to the involvement of GABAergic pathway.
Conclusion
Therefore, this study demonstrates the anxiolytic effect of CVO, in addition to providing a new nanoformulation for its administration.
Graphical Abstract</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>37639147</pmid><doi>10.1007/s40199-023-00473-z</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-0286-1974</orcidid><orcidid>https://orcid.org/0000-0003-4790-427X</orcidid><orcidid>https://orcid.org/0000-0001-9632-5464</orcidid><orcidid>https://orcid.org/0000-0002-2678-8188</orcidid><orcidid>https://orcid.org/0000-0002-4270-8109</orcidid><orcidid>https://orcid.org/0000-0002-8964-6398</orcidid><orcidid>https://orcid.org/0000-0001-6896-7179</orcidid><orcidid>https://orcid.org/0000-0001-5527-164X</orcidid><orcidid>https://orcid.org/0000-0003-0516-3143</orcidid><orcidid>https://orcid.org/0000-0003-1849-5403</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2008-2231 |
| ispartof | Daru, 2023-08, Vol.31 (2), p.183-192 |
| issn | 2008-2231 1560-8115 2008-2231 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10624781 |
| source | MEDLINE; SpringerLink Journals; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Anti-Anxiety Agents - metabolism Anti-Anxiety Agents - pharmacology Antianxiety agents Biomedical and Life Sciences Biomedicine Clove Oil - metabolism Clove Oil - pharmacology Eugenol - metabolism Eugenol - pharmacology Flumazenil GABA Medicinal Chemistry Nanoemulsions Oils, Volatile - pharmacology Pharmaceutical Sciences/Technology Pharmacology/Toxicology Polysaccharides Research Article Syzygium - metabolism Toxicity Zebrafish |
| title | Clove volatile oil-loaded nanoemulsion reduces the anxious-like behavior in adult zebrafish |
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