Hetong decoction relieves loperamide-induced constipation in rats by regulating expression of aquaporins

To investigate whether Hetong decoction (, HTT) alleviates constipation regulating AQPs expression. Constipation in rats was induced by loperamide, and rats were randomly assigned into model (saline), HHT-low (95 g/kg), HTT-medium (190 g/kg), HTT-high (380 g/kg) and positive control (mosapride) grou...

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Veröffentlicht in:	Journal of traditional Chinese medicine 2023-10, Vol.43 (6), p.1160-1167
Hauptverfasser:	Xi, Huang, Youguang, Xia, Kai, Han, Weiwei, Q I, Qing, Linghu, Qing, Zhang, Jingbo, Xue
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aquaporins - genetics Aquaporins - metabolism Colon - metabolism Constipation - chemically induced Constipation - drug therapy Constipation - genetics Cyclic AMP - genetics Cyclic AMP - metabolism Intestines Loperamide - adverse effects Loperamide - metabolism Rats
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container_title	Journal of traditional Chinese medicine
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creator	Xi, Huang Youguang, Xia Kai, Han Weiwei, Q I Qing, Linghu Qing, Zhang Jingbo, Xue
description	To investigate whether Hetong decoction (, HTT) alleviates constipation regulating AQPs expression. Constipation in rats was induced by loperamide, and rats were randomly assigned into model (saline), HHT-low (95 g/kg), HTT-medium (190 g/kg), HTT-high (380 g/kg) and positive control (mosapride) groups. Then the defecation function, the concentration of serum arginine vasopressin (AVP) and cyclic adenosine monophosphate (cAMP), and the expression of AQP3 and AQP8 in colon tissues were assessed. NCM460 colon cells with AQP3 and AQP8 knockdown or overexpression were exposed to serum from rats that received low or high dose of HTT, followed by detection of AQP3 and AQP8 expression. The model group showed lower fecal weight and water content, weaker intestinal transit, higher serum concentration of AVP and cAMP, increased proximal and distal AQP8 expression, increased proximal but decreased distal AQP3 expression. However, these trends were reversed in both the HTT group (low, medium and high dose) and the positive control group. In NCM460 cells, HTT dose-dependently stabilized AQP3 and AQP8 expression under AQP3/8 plasmid interference or overexpression. HTT relieves constipation in rats through regulating AQP3 and AQP8 expression.
doi_str_mv	10.19852/j.cnki.jtcm.2023.06.008
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Constipation in rats was induced by loperamide, and rats were randomly assigned into model (saline), HHT-low (95 g/kg), HTT-medium (190 g/kg), HTT-high (380 g/kg) and positive control (mosapride) groups. Then the defecation function, the concentration of serum arginine vasopressin (AVP) and cyclic adenosine monophosphate (cAMP), and the expression of AQP3 and AQP8 in colon tissues were assessed. NCM460 colon cells with AQP3 and AQP8 knockdown or overexpression were exposed to serum from rats that received low or high dose of HTT, followed by detection of AQP3 and AQP8 expression. The model group showed lower fecal weight and water content, weaker intestinal transit, higher serum concentration of AVP and cAMP, increased proximal and distal AQP8 expression, increased proximal but decreased distal AQP3 expression. However, these trends were reversed in both the HTT group (low, medium and high dose) and the positive control group. 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subjects	Animals Aquaporins - genetics Aquaporins - metabolism Colon - metabolism Constipation - chemically induced Constipation - drug therapy Constipation - genetics Cyclic AMP - genetics Cyclic AMP - metabolism Intestines Loperamide - adverse effects Loperamide - metabolism Rats
title	Hetong decoction relieves loperamide-induced constipation in rats by regulating expression of aquaporins
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