Transient expression of chimeric genes delivered into pollen by microprojectile bombardment

Chimeric genes containing a pollen-specific promoter from tomato (Lycopersicon esculentum) or the CaMV35S promoter were transiently expressed following their introduction into tobacco (Nicotiana tabacum) pollen using high velocity microprojectiles. Transient expression of the microprojectile-introdu...

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Veröffentlicht in:	Plant physiology (Bethesda) 1989-12, Vol.91 (4), p.1270-1274
Hauptverfasser:	Twell, D. (USDA, ARS, Plant Gene Expression Center, Albany, CA), Klein, T.M, Fromm, M.E, McCormick, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Anthers Biological and medical sciences Biotechnology CHIMERE Communications DNA Fundamental and applied biological sciences. Psychology GENE GENES Genetic engineering Genetic engineering applications Genetic technics Genetics and breeding of economic plants INVESTIGACION Leaves LYCOPERSICON ESCULENTUM Methods. Procedures. Technologies Molecular Biology and Gene Regulation NICOTIANA TABACUM Petals Plant breeding: fundamental aspects and methodology Plant cells Plants Plasmids POLEN POLLEN Protoplasts QUIMERA RECHERCHE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transgenic plants
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Zusammenfassung:	Chimeric genes containing a pollen-specific promoter from tomato (Lycopersicon esculentum) or the CaMV35S promoter were transiently expressed following their introduction into tobacco (Nicotiana tabacum) pollen using high velocity microprojectiles. Transient expression of the microprojectile-introduced genes in leaves and pollen was similar to that observed for these genes in stably transformed tobacco plants
ISSN:	0032-0889 1532-2548
DOI:	10.1104/pp.91.4.1270