Evidence for chloroplastic succinate dehydrogenase participating in the chloroplastic respiratory and photosynthetic electron transport chains of Chlamydomonas reinhardtii
A method for isolating intact chloroplasts from Chlamydomonas reinhardtii F-60 was developed from the Klein, Chen, Gibbs, Platt-Aloia procedure ([1983)] Plant Physiol 72: 481-487). Protoplasts, generated by treatment with autolysine, were lysed with a solution of digitonin and fractionated on Percol...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1989-07, Vol.90 (3), p.1084-1087 |
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| creator | Willeford, K.O. (Brandeis University, Waltham, MA) Gombos, Z Gibbs, M |
| description | A method for isolating intact chloroplasts from Chlamydomonas reinhardtii F-60 was developed from the Klein, Chen, Gibbs, Platt-Aloia procedure ([1983)] Plant Physiol 72: 481-487). Protoplasts, generated by treatment with autolysine, were lysed with a solution of digitonin and fractionated on Percoll step gradients. The chloroplasts were assessed to be 90% intact (ferricyanide assay) and free from cytoplasmic contamination (NADP isocitrate dehydrogenase activity) and to range from 2 to 5% in mitochondrial contamination (cytochrome c oxidase activity). About 25% of the cellular succinate dehydrogenase activity (21.6 micromoles per milligram chlorophyll per hour, as determined enzymically) was placed within the chloroplast. Chloroplastic succinate dehydrogenase had a Km for succinate of 0.55 millimolar and was associated with the thylakoidal material derived from the intact chloroplasts. This same thylakoidal material, with an enzymic assay of 21.6 micromoles per milligram chlorophyll per hour was able to initiate a light-dependent uptake of oxygen at a rate of 16.4 micromoles per milligram chlorophyll per hour when supplied with succinate and methyl viologen. Malonate was an apparent competitive inhibitor of this reaction. The succinate dehydrogenase activity present in the chloroplast was sufficient to account for the photoanaerobic rate of acetate dissimilation in H2 adapted Chlamydomonas (M Gibbs, RP Gfeller, C Chen [1986] Plant Physiol 82: 160-166) |
| doi_str_mv | 10.1104/pp.90.3.1084 |
| format | Article |
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(Brandeis University, Waltham, MA) ; Gombos, Z ; Gibbs, M</creator><creatorcontrib>Willeford, K.O. (Brandeis University, Waltham, MA) ; Gombos, Z ; Gibbs, M</creatorcontrib><description>A method for isolating intact chloroplasts from Chlamydomonas reinhardtii F-60 was developed from the Klein, Chen, Gibbs, Platt-Aloia procedure ([1983)] Plant Physiol 72: 481-487). Protoplasts, generated by treatment with autolysine, were lysed with a solution of digitonin and fractionated on Percoll step gradients. The chloroplasts were assessed to be 90% intact (ferricyanide assay) and free from cytoplasmic contamination (NADP isocitrate dehydrogenase activity) and to range from 2 to 5% in mitochondrial contamination (cytochrome c oxidase activity). About 25% of the cellular succinate dehydrogenase activity (21.6 micromoles per milligram chlorophyll per hour, as determined enzymically) was placed within the chloroplast. Chloroplastic succinate dehydrogenase had a Km for succinate of 0.55 millimolar and was associated with the thylakoidal material derived from the intact chloroplasts. This same thylakoidal material, with an enzymic assay of 21.6 micromoles per milligram chlorophyll per hour was able to initiate a light-dependent uptake of oxygen at a rate of 16.4 micromoles per milligram chlorophyll per hour when supplied with succinate and methyl viologen. Malonate was an apparent competitive inhibitor of this reaction. The succinate dehydrogenase activity present in the chloroplast was sufficient to account for the photoanaerobic rate of acetate dissimilation in H2 adapted Chlamydomonas (M Gibbs, RP Gfeller, C Chen [1986] Plant Physiol 82: 160-166)</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.90.3.1084</identifier><identifier>PMID: 16666855</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>551000 - Physiological Systems ; ACETATES ; ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ADAPTACION FISIOLOGICA ; ADAPTATION ; ALGAE ; ALGUE ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; CARBOXYLIC ACID SALTS ; CARBOXYLIC ACIDS ; CELL CONSTITUENTS ; CHEMICAL REACTIONS ; CHLAMYDOMONAS ; Chlamydomonas reinhardtii ; CHLOROPHYCOTA ; CHLOROPHYLLE ; CHLOROPLASTS ; CLOROFILAS ; CONSOMMATION D'OXYGENE ; CONSUMO DE OXIGENO ; Dehydrogenases ; DICARBOXYLIC ACIDS ; electron transport ; ENZYME ACTIVITY ; ENZYMES ; Freshwater ; Fundamental and applied biological sciences. Psychology ; HIDROGENO ; HYDROGENE ; Isocitrates ; Malonates ; Metabolism ; Metabolism and Enzymology ; METABOLISME ; METABOLISMO ; methodology ; MICROORGANISMS ; Mitochondria ; ORGANIC ACIDS ; ORGANIC COMPOUNDS ; Oxidases ; OXIDATION ; OXIDOREDUCTASES ; OXIDORREDUCTASAS ; OXYDOREDUCTASE ; PHOTOCHEMICAL REACTIONS ; PHOTOSYNTHESIS ; Plant cells ; Plant physiology and development ; PLANTS ; PLASTE ; PLASTIDIOS ; Protoplasts ; respiration ; separation ; succinate dehydrogenase ; SUCCINIC ACID ; SYNTHESIS ; TECHNIQUE DE L'ISOLEMENT ; TECNICAS DE AISLAMIENTO ; UNICELLULAR ALGAE</subject><ispartof>Plant physiology (Bethesda), 1989-07, Vol.90 (3), p.1084-1087</ispartof><rights>Copyright 1989 American Society of Plant Physiologists</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c610t-2fbf57b0f25d7cdb12e2d022f9d5fb9e1eec667b68e0a6f227d702f6bd9429623</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4272202$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4272202$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6587902$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16666855$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/7151146$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Willeford, K.O. (Brandeis University, Waltham, MA)</creatorcontrib><creatorcontrib>Gombos, Z</creatorcontrib><creatorcontrib>Gibbs, M</creatorcontrib><title>Evidence for chloroplastic succinate dehydrogenase participating in the chloroplastic respiratory and photosynthetic electron transport chains of Chlamydomonas reinhardtii</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>A method for isolating intact chloroplasts from Chlamydomonas reinhardtii F-60 was developed from the Klein, Chen, Gibbs, Platt-Aloia procedure ([1983)] Plant Physiol 72: 481-487). Protoplasts, generated by treatment with autolysine, were lysed with a solution of digitonin and fractionated on Percoll step gradients. The chloroplasts were assessed to be 90% intact (ferricyanide assay) and free from cytoplasmic contamination (NADP isocitrate dehydrogenase activity) and to range from 2 to 5% in mitochondrial contamination (cytochrome c oxidase activity). About 25% of the cellular succinate dehydrogenase activity (21.6 micromoles per milligram chlorophyll per hour, as determined enzymically) was placed within the chloroplast. Chloroplastic succinate dehydrogenase had a Km for succinate of 0.55 millimolar and was associated with the thylakoidal material derived from the intact chloroplasts. This same thylakoidal material, with an enzymic assay of 21.6 micromoles per milligram chlorophyll per hour was able to initiate a light-dependent uptake of oxygen at a rate of 16.4 micromoles per milligram chlorophyll per hour when supplied with succinate and methyl viologen. Malonate was an apparent competitive inhibitor of this reaction. The succinate dehydrogenase activity present in the chloroplast was sufficient to account for the photoanaerobic rate of acetate dissimilation in H2 adapted Chlamydomonas (M Gibbs, RP Gfeller, C Chen [1986] Plant Physiol 82: 160-166)</description><subject>551000 - Physiological Systems</subject><subject>ACETATES</subject><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ADAPTACION FISIOLOGICA</subject><subject>ADAPTATION</subject><subject>ALGAE</subject><subject>ALGUE</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>CARBOXYLIC ACID SALTS</subject><subject>CARBOXYLIC ACIDS</subject><subject>CELL CONSTITUENTS</subject><subject>CHEMICAL REACTIONS</subject><subject>CHLAMYDOMONAS</subject><subject>Chlamydomonas reinhardtii</subject><subject>CHLOROPHYCOTA</subject><subject>CHLOROPHYLLE</subject><subject>CHLOROPLASTS</subject><subject>CLOROFILAS</subject><subject>CONSOMMATION D'OXYGENE</subject><subject>CONSUMO DE OXIGENO</subject><subject>Dehydrogenases</subject><subject>DICARBOXYLIC ACIDS</subject><subject>electron transport</subject><subject>ENZYME ACTIVITY</subject><subject>ENZYMES</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HIDROGENO</subject><subject>HYDROGENE</subject><subject>Isocitrates</subject><subject>Malonates</subject><subject>Metabolism</subject><subject>Metabolism and Enzymology</subject><subject>METABOLISME</subject><subject>METABOLISMO</subject><subject>methodology</subject><subject>MICROORGANISMS</subject><subject>Mitochondria</subject><subject>ORGANIC ACIDS</subject><subject>ORGANIC COMPOUNDS</subject><subject>Oxidases</subject><subject>OXIDATION</subject><subject>OXIDOREDUCTASES</subject><subject>OXIDORREDUCTASAS</subject><subject>OXYDOREDUCTASE</subject><subject>PHOTOCHEMICAL REACTIONS</subject><subject>PHOTOSYNTHESIS</subject><subject>Plant cells</subject><subject>Plant physiology and development</subject><subject>PLANTS</subject><subject>PLASTE</subject><subject>PLASTIDIOS</subject><subject>Protoplasts</subject><subject>respiration</subject><subject>separation</subject><subject>succinate dehydrogenase</subject><subject>SUCCINIC ACID</subject><subject>SYNTHESIS</subject><subject>TECHNIQUE DE L'ISOLEMENT</subject><subject>TECNICAS DE AISLAMIENTO</subject><subject>UNICELLULAR ALGAE</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNp9ksuKFDEUhgtRnHF050pEChHd2G2SqiSVjSDNeIEBFzrrkEpOujJUJ5kkPdDP5Euatpv2sjCbBP7v_OeS0zRPMVpijPp3MS4FWnZLjIb-XnOOaUcWhPbD_eYcofpGwyDOmkc53yCEcIf7h80ZZvUMlJ43Py7vnAGvobUhtXqaQwpxVrk43eat1s6rAq2BaWdSWINXGdqoUpVdVMX5det8Wyb4JzRBji6pEtKuVd60cQol5J2v5F6GGXRJoUYm5XMMqdR45Xxug21X06w2OxM2oWarTs5PKpni3OPmgVVzhifH-6K5_nj5ffV5cfX105fVh6uFZhiVBbGjpXxEllDDtRkxAWIQIVYYakcBGEAzxkc2AFLMEsINR8Sy0YieCEa6i-b9wTduxw0YDb6WOcuY3EalnQzKyb8V7ya5DncSI4aHnleDlweDUIchs3YF9KSD97VryTHFuGcVenPMksLtFnKRG5c1zLPyELZZ8q7rRUe4qOTr_5KYCsaHfqjg2wOoU8g5gT3VjJHcL4uMUQokO7lfloq_-LPP3_BxOyrw6giorNVs62dpl08cowMXaD-v5wfsJtcPP8k94YT8kp8dZKuCVOtUHa6_CYQpp6z7CXFZ4DM</recordid><startdate>19890701</startdate><enddate>19890701</enddate><creator>Willeford, K.O. 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Psychology</topic><topic>HIDROGENO</topic><topic>HYDROGENE</topic><topic>Isocitrates</topic><topic>Malonates</topic><topic>Metabolism</topic><topic>Metabolism and Enzymology</topic><topic>METABOLISME</topic><topic>METABOLISMO</topic><topic>methodology</topic><topic>MICROORGANISMS</topic><topic>Mitochondria</topic><topic>ORGANIC ACIDS</topic><topic>ORGANIC COMPOUNDS</topic><topic>Oxidases</topic><topic>OXIDATION</topic><topic>OXIDOREDUCTASES</topic><topic>OXIDORREDUCTASAS</topic><topic>OXYDOREDUCTASE</topic><topic>PHOTOCHEMICAL REACTIONS</topic><topic>PHOTOSYNTHESIS</topic><topic>Plant cells</topic><topic>Plant physiology and development</topic><topic>PLANTS</topic><topic>PLASTE</topic><topic>PLASTIDIOS</topic><topic>Protoplasts</topic><topic>respiration</topic><topic>separation</topic><topic>succinate dehydrogenase</topic><topic>SUCCINIC ACID</topic><topic>SYNTHESIS</topic><topic>TECHNIQUE DE L'ISOLEMENT</topic><topic>TECNICAS DE AISLAMIENTO</topic><topic>UNICELLULAR ALGAE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Willeford, K.O. (Brandeis University, Waltham, MA)</creatorcontrib><creatorcontrib>Gombos, Z</creatorcontrib><creatorcontrib>Gibbs, M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Willeford, K.O. (Brandeis University, Waltham, MA)</au><au>Gombos, Z</au><au>Gibbs, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for chloroplastic succinate dehydrogenase participating in the chloroplastic respiratory and photosynthetic electron transport chains of Chlamydomonas reinhardtii</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1989-07-01</date><risdate>1989</risdate><volume>90</volume><issue>3</issue><spage>1084</spage><epage>1087</epage><pages>1084-1087</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>A method for isolating intact chloroplasts from Chlamydomonas reinhardtii F-60 was developed from the Klein, Chen, Gibbs, Platt-Aloia procedure ([1983)] Plant Physiol 72: 481-487). Protoplasts, generated by treatment with autolysine, were lysed with a solution of digitonin and fractionated on Percoll step gradients. The chloroplasts were assessed to be 90% intact (ferricyanide assay) and free from cytoplasmic contamination (NADP isocitrate dehydrogenase activity) and to range from 2 to 5% in mitochondrial contamination (cytochrome c oxidase activity). About 25% of the cellular succinate dehydrogenase activity (21.6 micromoles per milligram chlorophyll per hour, as determined enzymically) was placed within the chloroplast. Chloroplastic succinate dehydrogenase had a Km for succinate of 0.55 millimolar and was associated with the thylakoidal material derived from the intact chloroplasts. This same thylakoidal material, with an enzymic assay of 21.6 micromoles per milligram chlorophyll per hour was able to initiate a light-dependent uptake of oxygen at a rate of 16.4 micromoles per milligram chlorophyll per hour when supplied with succinate and methyl viologen. Malonate was an apparent competitive inhibitor of this reaction. The succinate dehydrogenase activity present in the chloroplast was sufficient to account for the photoanaerobic rate of acetate dissimilation in H2 adapted Chlamydomonas (M Gibbs, RP Gfeller, C Chen [1986] Plant Physiol 82: 160-166)</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16666855</pmid><doi>10.1104/pp.90.3.1084</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1989-07, Vol.90 (3), p.1084-1087 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1061847 |
| source | Jstor Complete Legacy; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 551000 - Physiological Systems ACETATES ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ADAPTACION FISIOLOGICA ADAPTATION ALGAE ALGUE BASIC BIOLOGICAL SCIENCES Biological and medical sciences CARBOXYLIC ACID SALTS CARBOXYLIC ACIDS CELL CONSTITUENTS CHEMICAL REACTIONS CHLAMYDOMONAS Chlamydomonas reinhardtii CHLOROPHYCOTA CHLOROPHYLLE CHLOROPLASTS CLOROFILAS CONSOMMATION D'OXYGENE CONSUMO DE OXIGENO Dehydrogenases DICARBOXYLIC ACIDS electron transport ENZYME ACTIVITY ENZYMES Freshwater Fundamental and applied biological sciences. Psychology HIDROGENO HYDROGENE Isocitrates Malonates Metabolism Metabolism and Enzymology METABOLISME METABOLISMO methodology MICROORGANISMS Mitochondria ORGANIC ACIDS ORGANIC COMPOUNDS Oxidases OXIDATION OXIDOREDUCTASES OXIDORREDUCTASAS OXYDOREDUCTASE PHOTOCHEMICAL REACTIONS PHOTOSYNTHESIS Plant cells Plant physiology and development PLANTS PLASTE PLASTIDIOS Protoplasts respiration separation succinate dehydrogenase SUCCINIC ACID SYNTHESIS TECHNIQUE DE L'ISOLEMENT TECNICAS DE AISLAMIENTO UNICELLULAR ALGAE |
| title | Evidence for chloroplastic succinate dehydrogenase participating in the chloroplastic respiratory and photosynthetic electron transport chains of Chlamydomonas reinhardtii |
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