The Effects of Probiotic Bacillus Spores on Dexamethasone-Treated Rats
Glucocorticoids are effective anti-inflammatory and immunosuppressive agents. Long-term exposure is associated with multiple metabolic side effects. Spore-forming probiotic bacteria have shown modulatory properties regarding glycolipid metabolism and inflammation. The aim of this study was to evalua...
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| Veröffentlicht in: | International journal of molecular sciences 2023-10, Vol.24 (20), p.15111 |
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| creator | Inceu, Andreea Ioana Neag, Maria Adriana Catinean, Adrian Bocsan, Corina Ioana Craciun, Cristian Ioan Melincovici, Carmen Stanca Muntean, Dana Maria Onofrei, Mădălin Mihai Pop, Raluca Maria Buzoianu, Anca Dana |
| description | Glucocorticoids are effective anti-inflammatory and immunosuppressive agents. Long-term exposure is associated with multiple metabolic side effects. Spore-forming probiotic bacteria have shown modulatory properties regarding glycolipid metabolism and inflammation. The aim of this study was to evaluate, for the first time, the effects of Bacillus species spores (B. licheniformis, B. indicus, B. subtilis, B. clausii, and B. coagulans) alone and in combination with metformin against dexamethasone-induced systemic disturbances. A total of 30 rats were randomly divided into 5 groups: group 1 served as control (CONTROL), group 2 received dexamethasone (DEXA), group 3 received DEXA and MegaSporeBiotic (MSB), group 4 received DEXA and metformin (MET), and group 5 received DEXA, MSB, and MET. On the last day of the experiment, blood samples and liver tissue samples for histopathological examination were collected. We determined serum glucose, total cholesterol, triglycerides, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), catalase, total antioxidant capacity (TAC), and metformin concentration. DEXA administration caused hyperglycemia and hyperlipidemia, increased inflammation cytokines, and decreased antioxidant markers. Treatment with MSB reduced total cholesterol, suggesting that the administration of Bacillus spores-based probiotics to DEXA-treated rats could ameliorate metabolic parameters. |
| doi_str_mv | 10.3390/ijms242015111 |
| format | Article |
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Long-term exposure is associated with multiple metabolic side effects. Spore-forming probiotic bacteria have shown modulatory properties regarding glycolipid metabolism and inflammation. The aim of this study was to evaluate, for the first time, the effects of Bacillus species spores (B. licheniformis, B. indicus, B. subtilis, B. clausii, and B. coagulans) alone and in combination with metformin against dexamethasone-induced systemic disturbances. A total of 30 rats were randomly divided into 5 groups: group 1 served as control (CONTROL), group 2 received dexamethasone (DEXA), group 3 received DEXA and MegaSporeBiotic (MSB), group 4 received DEXA and metformin (MET), and group 5 received DEXA, MSB, and MET. On the last day of the experiment, blood samples and liver tissue samples for histopathological examination were collected. We determined serum glucose, total cholesterol, triglycerides, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), catalase, total antioxidant capacity (TAC), and metformin concentration. DEXA administration caused hyperglycemia and hyperlipidemia, increased inflammation cytokines, and decreased antioxidant markers. Treatment with MSB reduced total cholesterol, suggesting that the administration of Bacillus spores-based probiotics to DEXA-treated rats could ameliorate metabolic parameters.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms242015111</identifier><identifier>PMID: 37894792</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Apoptosis ; Cholesterol ; Coronaviruses ; COVID-19 ; Diabetes ; Glucose ; Hyperglycemia ; Inflammation ; Insulin resistance ; Lipids ; Liver ; Metabolic disorders ; Metabolism ; Microbiota ; Probiotics ; Triglycerides</subject><ispartof>International journal of molecular sciences, 2023-10, Vol.24 (20), p.15111</ispartof><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). 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Long-term exposure is associated with multiple metabolic side effects. Spore-forming probiotic bacteria have shown modulatory properties regarding glycolipid metabolism and inflammation. The aim of this study was to evaluate, for the first time, the effects of Bacillus species spores (B. licheniformis, B. indicus, B. subtilis, B. clausii, and B. coagulans) alone and in combination with metformin against dexamethasone-induced systemic disturbances. A total of 30 rats were randomly divided into 5 groups: group 1 served as control (CONTROL), group 2 received dexamethasone (DEXA), group 3 received DEXA and MegaSporeBiotic (MSB), group 4 received DEXA and metformin (MET), and group 5 received DEXA, MSB, and MET. On the last day of the experiment, blood samples and liver tissue samples for histopathological examination were collected. We determined serum glucose, total cholesterol, triglycerides, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), catalase, total antioxidant capacity (TAC), and metformin concentration. DEXA administration caused hyperglycemia and hyperlipidemia, increased inflammation cytokines, and decreased antioxidant markers. Treatment with MSB reduced total cholesterol, suggesting that the administration of Bacillus spores-based probiotics to DEXA-treated rats could ameliorate metabolic parameters.</description><subject>Apoptosis</subject><subject>Cholesterol</subject><subject>Coronaviruses</subject><subject>COVID-19</subject><subject>Diabetes</subject><subject>Glucose</subject><subject>Hyperglycemia</subject><subject>Inflammation</subject><subject>Insulin resistance</subject><subject>Lipids</subject><subject>Liver</subject><subject>Metabolic disorders</subject><subject>Metabolism</subject><subject>Microbiota</subject><subject>Probiotics</subject><subject>Triglycerides</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkU1PwzAMhiMEYmNw5F6JC5dCPpo2OSEYGyBNAsE4R1nqsE5tM5IUwb-n0xBiHCxb9qNXtl-ETgm-YEziy2rVBJpRTDghZA8NSUZpinFe7P-pB-gohBXGlFEuD9GAFUJmhaRDNJ0vIZlYCyaGxNnkybtF5WJlkhttqrruQvKydh76YZvcwqduIC51cC2kcw86Qpk86xiO0YHVdYCTnzxCr9PJfHyfzh7vHsbXs9SwTMaUMVHQgnFtZE6NEGUGC8K5NFxwTKQlltpsIYDi3ApalLLMBcXWyL7Xh2EjdLXVXXeLBkoDbfS6VmtfNdp_KacrtTtpq6V6cx-K4Bznsn_ACJ3_KHj33kGIqqmCgbrWLbguKCoE4yITfIOe_UNXrvNtf9-G6j_JGcY9lW4p410IHuzvNgSrjUVqxyL2DbFsghc</recordid><startdate>20231012</startdate><enddate>20231012</enddate><creator>Inceu, Andreea Ioana</creator><creator>Neag, Maria Adriana</creator><creator>Catinean, Adrian</creator><creator>Bocsan, Corina Ioana</creator><creator>Craciun, Cristian Ioan</creator><creator>Melincovici, Carmen Stanca</creator><creator>Muntean, Dana Maria</creator><creator>Onofrei, Mădălin Mihai</creator><creator>Pop, Raluca Maria</creator><creator>Buzoianu, Anca Dana</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1899-5977</orcidid><orcidid>https://orcid.org/0000-0002-6327-6608</orcidid><orcidid>https://orcid.org/0000-0002-3279-5384</orcidid><orcidid>https://orcid.org/0000-0001-8863-2851</orcidid><orcidid>https://orcid.org/0000-0002-3904-2852</orcidid><orcidid>https://orcid.org/0000-0002-3914-0110</orcidid><orcidid>https://orcid.org/0000-0002-3511-2788</orcidid></search><sort><creationdate>20231012</creationdate><title>The Effects of Probiotic Bacillus Spores on Dexamethasone-Treated Rats</title><author>Inceu, Andreea Ioana ; 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Long-term exposure is associated with multiple metabolic side effects. Spore-forming probiotic bacteria have shown modulatory properties regarding glycolipid metabolism and inflammation. The aim of this study was to evaluate, for the first time, the effects of Bacillus species spores (B. licheniformis, B. indicus, B. subtilis, B. clausii, and B. coagulans) alone and in combination with metformin against dexamethasone-induced systemic disturbances. A total of 30 rats were randomly divided into 5 groups: group 1 served as control (CONTROL), group 2 received dexamethasone (DEXA), group 3 received DEXA and MegaSporeBiotic (MSB), group 4 received DEXA and metformin (MET), and group 5 received DEXA, MSB, and MET. On the last day of the experiment, blood samples and liver tissue samples for histopathological examination were collected. We determined serum glucose, total cholesterol, triglycerides, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), catalase, total antioxidant capacity (TAC), and metformin concentration. DEXA administration caused hyperglycemia and hyperlipidemia, increased inflammation cytokines, and decreased antioxidant markers. 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| subjects | Apoptosis Cholesterol Coronaviruses COVID-19 Diabetes Glucose Hyperglycemia Inflammation Insulin resistance Lipids Liver Metabolic disorders Metabolism Microbiota Probiotics Triglycerides |
| title | The Effects of Probiotic Bacillus Spores on Dexamethasone-Treated Rats |
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