Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent RNA processing

Non-cleaving Cas9 (dCas9) is widely employed to manipulate specific gene loci, often with scant regard for unintended transcriptional effects. We demonstrate here that dCas9 mediates precise RNA polymerase II transcriptional pausing followed by transcription termination and potential alternative pol...

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Veröffentlicht in:	Nature structural & molecular biology 2023-10, Vol.30 (10), p.1536-1548
Hauptverfasser:	Zukher, Inna, Dujardin, Gwendal, Sousa-Luís, Rui, Proudfoot, Nick J.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/337 631/337/572 631/337/572/2102 Alternative Splicing Animals Annealing Biochemistry Biological Microscopy Biomedical and Life Sciences DNA-directed RNA polymerase Elongation Humans Life Sciences Mammals - genetics Membrane Biology mRNA processing Orientation effects Polyadenylation Post-transcription Protein Structure Ribonucleic acid RNA RNA polymerase RNA polymerase II RNA Polymerase II - genetics RNA Polymerase II - metabolism RNA processing Transcription elongation Transcription termination Transcription, Genetic
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