CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering

Existing technologies for site-specific integration of kilobase-sized DNA sequences in bacteria are limited by low efficiency, a reliance on recombination, the need for multiple vectors, and challenges in multiplexing. To address these shortcomings, we introduce a substantially improved version of o...

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Veröffentlicht in:	Nature biotechnology 2021-04, Vol.39 (4), p.480-489
Hauptverfasser:	Vo, Phuc Leo H., Ronda, Carlotta, Klompe, Sanne E., Chen, Ethan E., Acree, Christopher, Wang, Harris H., Sternberg, Samuel H.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/337 631/61 Agriculture Bacteria Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Control CRISPR CRISPR-Cas Systems Deoxyribonucleic acid DNA DNA Transposable Elements Efficiency Gene Editing - methods Gene sequencing Gene therapy Genome editing Genome, Bacterial Genomes Integrases Life Sciences Loci Mathematical analysis Methods Microbial genetic engineering Multiplexing Nucleotide sequence Plasmids - genetics Recombination Ribonucleic acid RNA RNA, Guide, CRISPR-Cas Systems - genetics Transposition Transposons Vibrio cholerae - genetics
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