Immunohistochemical evaluation of superficial and invasive front of oral squamous cell carcinoma using epithelial membrane antigen as a marker
Background: Epithelial membrane antigen (EMA) has been used as a marker for the expression of tumour margins in various glandular neoplastic lesions. Histopathologically, oral squamous cell carcinoma (OSCC) may exhibit several features within the same tumour cells, portraying that these cells at the...
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| Veröffentlicht in: | Journal of oral and maxillofacial pathology : JOMFP 2023-04, Vol.27 (2), p.427-427 |
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| container_title | Journal of oral and maxillofacial pathology : JOMFP |
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| creator | Jayaswal, Amrita Tandon, Aanchal Jain, Amol Sharma, Apoorva Iqbal, Haider Jaiswal, Rohit |
| description | Background: Epithelial membrane antigen (EMA) has been used as a marker for the expression of tumour margins in various glandular neoplastic lesions. Histopathologically, oral squamous cell carcinoma (OSCC) may exhibit several features within the same tumour cells, portraying that these cells at the invasive margins commonly display certain features that differ from those of the superficial part of the tumour.
Aim: To identify and study the invasive tumour front and also to recognise any micrometastases in an OSCC lesion.
Materials and Method: A retrospective study of 30 OSCC cases with superficial and most invasive parts were sectioned at 4 μm. Routine H&E staining and immunohistochemical staining with mouse antihuman EMA were done. The OSCC cases were graded into well differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma (MDSCC) and poorly differentiated squamous cell carcinoma (PDSCC). The EMA-stained slides were observed and analysed under higher magnification to identify the individual EMA-stained cells.
Results: Analysis of Variance (ANOVA) analysis revealed that when comparing the superficial and invasive fronts of OSCC, it was evident that the P values were significant across the groups. In WDSCC, positive predictive value was 70.6% and sensitivity was 100% when the same slide was analysed for large and small islands to individual cells in an EMA-stained section, while MDSCC and PDSCC showed both sensitivity and positive predictive value to be 100%.
Conclusion: EMA could be considered a useful prognostic marker for describing the nature of the neoplastic epithelium as well as recognising the typical anaplastic cells in cases of OSCC. |
| doi_str_mv | 10.4103/jomfp.jomfp_295_22 |
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Aim: To identify and study the invasive tumour front and also to recognise any micrometastases in an OSCC lesion.
Materials and Method: A retrospective study of 30 OSCC cases with superficial and most invasive parts were sectioned at 4 μm. Routine H&E staining and immunohistochemical staining with mouse antihuman EMA were done. The OSCC cases were graded into well differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma (MDSCC) and poorly differentiated squamous cell carcinoma (PDSCC). The EMA-stained slides were observed and analysed under higher magnification to identify the individual EMA-stained cells.
Results: Analysis of Variance (ANOVA) analysis revealed that when comparing the superficial and invasive fronts of OSCC, it was evident that the P values were significant across the groups. In WDSCC, positive predictive value was 70.6% and sensitivity was 100% when the same slide was analysed for large and small islands to individual cells in an EMA-stained section, while MDSCC and PDSCC showed both sensitivity and positive predictive value to be 100%.
Conclusion: EMA could be considered a useful prognostic marker for describing the nature of the neoplastic epithelium as well as recognising the typical anaplastic cells in cases of OSCC.</description><identifier>ISSN: 0973-029X</identifier><identifier>EISSN: 1998-393X</identifier><identifier>DOI: 10.4103/jomfp.jomfp_295_22</identifier><language>eng</language><publisher>Chennai: Wolters Kluwer India Pvt. Ltd</publisher><subject>Analysis ; Antigens ; Cancer ; Cell differentiation ; Development and progression ; Epithelium ; Invasiveness ; Oral cancer ; Oral carcinoma ; Oral squamous cell carcinoma ; Original ; Squamous cell carcinoma ; Tumors ; Variance analysis</subject><ispartof>Journal of oral and maxillofacial pathology : JOMFP, 2023-04, Vol.27 (2), p.427-427</ispartof><rights>COPYRIGHT 2023 Medknow Publications and Media Pvt. Ltd.</rights><rights>2023. This article is published under (http://creativecommons.org/licenses/by-nc-sa/3.0/) (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright: © 2023 Journal of Oral and Maxillofacial Pathology 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c430i-d5789860730efdef2af4de425fd1660cfb6ebe1bed31f0b0419cf23e634a12863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581295/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581295/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27457,27923,27924,53790,53792</link.rule.ids></links><search><creatorcontrib>Jayaswal, Amrita</creatorcontrib><creatorcontrib>Tandon, Aanchal</creatorcontrib><creatorcontrib>Jain, Amol</creatorcontrib><creatorcontrib>Sharma, Apoorva</creatorcontrib><creatorcontrib>Iqbal, Haider</creatorcontrib><creatorcontrib>Jaiswal, Rohit</creatorcontrib><title>Immunohistochemical evaluation of superficial and invasive front of oral squamous cell carcinoma using epithelial membrane antigen as a marker</title><title>Journal of oral and maxillofacial pathology : JOMFP</title><description>Background: Epithelial membrane antigen (EMA) has been used as a marker for the expression of tumour margins in various glandular neoplastic lesions. Histopathologically, oral squamous cell carcinoma (OSCC) may exhibit several features within the same tumour cells, portraying that these cells at the invasive margins commonly display certain features that differ from those of the superficial part of the tumour.
Aim: To identify and study the invasive tumour front and also to recognise any micrometastases in an OSCC lesion.
Materials and Method: A retrospective study of 30 OSCC cases with superficial and most invasive parts were sectioned at 4 μm. Routine H&E staining and immunohistochemical staining with mouse antihuman EMA were done. The OSCC cases were graded into well differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma (MDSCC) and poorly differentiated squamous cell carcinoma (PDSCC). The EMA-stained slides were observed and analysed under higher magnification to identify the individual EMA-stained cells.
Results: Analysis of Variance (ANOVA) analysis revealed that when comparing the superficial and invasive fronts of OSCC, it was evident that the P values were significant across the groups. In WDSCC, positive predictive value was 70.6% and sensitivity was 100% when the same slide was analysed for large and small islands to individual cells in an EMA-stained section, while MDSCC and PDSCC showed both sensitivity and positive predictive value to be 100%.
Conclusion: EMA could be considered a useful prognostic marker for describing the nature of the neoplastic epithelium as well as recognising the typical anaplastic cells in cases of OSCC.</description><subject>Analysis</subject><subject>Antigens</subject><subject>Cancer</subject><subject>Cell differentiation</subject><subject>Development and progression</subject><subject>Epithelium</subject><subject>Invasiveness</subject><subject>Oral cancer</subject><subject>Oral carcinoma</subject><subject>Oral squamous cell carcinoma</subject><subject>Original</subject><subject>Squamous cell carcinoma</subject><subject>Tumors</subject><subject>Variance analysis</subject><issn>0973-029X</issn><issn>1998-393X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9UsuKFDEULUTBtvUHXAXcuOk2r3qtZBhfAwNuFGYXUqmb7nRXkp6kqht_wm_2lj0OjsgQSCD3nJOcc29RvGZ0LRkV73bR28P69654WyrOnxQL1rbNSrTi5mmxoG0tVpS3N8-LFznvKC0bWfJF8fPK-ynErctjNFvwzuiBwFEPkx5dDCRakqcDJOuMw4oOPXHhqLM7ArEphnFGxISlfDtpH6dMDAwDMToZF6LXZMoubAgc3LiFYdbw4LukA6DY6DYQiM5EE6_THtLL4pnVQ4ZXd-ey-P7p47fLL6vrr5-vLi-uV0YK6lZ9WTdtU9FaULA9WK6t7EHy0vasqqixXQUdsA56wSztqGStsVxAJaRmvKnEsnh_1j1MnYfeQBjRgzokh__4oaJ26mEluK3axKNiGBzDhFHh7Z1CircT5FF5l2fr6AxTULypW0lrhrkvizf_QHdxSgH9KcGkrBlu9DEUb2TVtLLFXt6jNnoA5YKN-D0zP60u6opih9Erotb_QeHq5w7HANbh_QMCPxNMijknsPdRMKrmCVPn2fp7wpD04Uw6xWGElPfDdIKkMLV9iKdHmEryWv0ZPPEL4H_jAA</recordid><startdate>20230401</startdate><enddate>20230401</enddate><creator>Jayaswal, Amrita</creator><creator>Tandon, Aanchal</creator><creator>Jain, Amol</creator><creator>Sharma, Apoorva</creator><creator>Iqbal, Haider</creator><creator>Jaiswal, Rohit</creator><general>Wolters Kluwer India Pvt. 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Tandon, Aanchal ; Jain, Amol ; Sharma, Apoorva ; Iqbal, Haider ; Jaiswal, Rohit</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430i-d5789860730efdef2af4de425fd1660cfb6ebe1bed31f0b0419cf23e634a12863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Analysis</topic><topic>Antigens</topic><topic>Cancer</topic><topic>Cell differentiation</topic><topic>Development and progression</topic><topic>Epithelium</topic><topic>Invasiveness</topic><topic>Oral cancer</topic><topic>Oral carcinoma</topic><topic>Oral squamous cell carcinoma</topic><topic>Original</topic><topic>Squamous cell carcinoma</topic><topic>Tumors</topic><topic>Variance analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jayaswal, Amrita</creatorcontrib><creatorcontrib>Tandon, Aanchal</creatorcontrib><creatorcontrib>Jain, Amol</creatorcontrib><creatorcontrib>Sharma, Apoorva</creatorcontrib><creatorcontrib>Iqbal, Haider</creatorcontrib><creatorcontrib>Jaiswal, Rohit</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of oral and maxillofacial pathology : JOMFP</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jayaswal, Amrita</au><au>Tandon, Aanchal</au><au>Jain, Amol</au><au>Sharma, Apoorva</au><au>Iqbal, Haider</au><au>Jaiswal, Rohit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical evaluation of superficial and invasive front of oral squamous cell carcinoma using epithelial membrane antigen as a marker</atitle><jtitle>Journal of oral and maxillofacial pathology : JOMFP</jtitle><date>2023-04-01</date><risdate>2023</risdate><volume>27</volume><issue>2</issue><spage>427</spage><epage>427</epage><pages>427-427</pages><issn>0973-029X</issn><eissn>1998-393X</eissn><abstract>Background: Epithelial membrane antigen (EMA) has been used as a marker for the expression of tumour margins in various glandular neoplastic lesions. Histopathologically, oral squamous cell carcinoma (OSCC) may exhibit several features within the same tumour cells, portraying that these cells at the invasive margins commonly display certain features that differ from those of the superficial part of the tumour.
Aim: To identify and study the invasive tumour front and also to recognise any micrometastases in an OSCC lesion.
Materials and Method: A retrospective study of 30 OSCC cases with superficial and most invasive parts were sectioned at 4 μm. Routine H&E staining and immunohistochemical staining with mouse antihuman EMA were done. The OSCC cases were graded into well differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma (MDSCC) and poorly differentiated squamous cell carcinoma (PDSCC). The EMA-stained slides were observed and analysed under higher magnification to identify the individual EMA-stained cells.
Results: Analysis of Variance (ANOVA) analysis revealed that when comparing the superficial and invasive fronts of OSCC, it was evident that the P values were significant across the groups. In WDSCC, positive predictive value was 70.6% and sensitivity was 100% when the same slide was analysed for large and small islands to individual cells in an EMA-stained section, while MDSCC and PDSCC showed both sensitivity and positive predictive value to be 100%.
Conclusion: EMA could be considered a useful prognostic marker for describing the nature of the neoplastic epithelium as well as recognising the typical anaplastic cells in cases of OSCC.</abstract><cop>Chennai</cop><pub>Wolters Kluwer India Pvt. Ltd</pub><doi>10.4103/jomfp.jomfp_295_22</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of oral and maxillofacial pathology : JOMFP, 2023-04, Vol.27 (2), p.427-427 |
| issn | 0973-029X 1998-393X |
| language | eng |
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| source | Medknow Open Access Medical Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Analysis Antigens Cancer Cell differentiation Development and progression Epithelium Invasiveness Oral cancer Oral carcinoma Oral squamous cell carcinoma Original Squamous cell carcinoma Tumors Variance analysis |
| title | Immunohistochemical evaluation of superficial and invasive front of oral squamous cell carcinoma using epithelial membrane antigen as a marker |
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