Properties of Degradable Polyhydroxyalkanoates Synthesized from New Waste Fish Oils (WFOs)
The synthesis of PHA was first investigated using WFOs obtained from smoked-sprat heads, substandard fresh sprats, and fresh mackerel heads and backbones. All the WFOs ensured the growth of the wild-type strain Cupriavidus necator B-10646 and the synthesis of PHA, regardless of the degree of lipid s...
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| Veröffentlicht in: | International journal of molecular sciences 2023-10, Vol.24 (19), p.14919 |
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| creator | Zhila, Natalia O. Kiselev, Evgeniy G. Volkov, Vladimir V. Mezenova, Olga Ya Sapozhnikova, Kristina Yu Shishatskaya, Ekaterina I. Volova, Tatiana G. |
| description | The synthesis of PHA was first investigated using WFOs obtained from smoked-sprat heads, substandard fresh sprats, and fresh mackerel heads and backbones. All the WFOs ensured the growth of the wild-type strain Cupriavidus necator B-10646 and the synthesis of PHA, regardless of the degree of lipid saturation (from 0.52 to 0.65) and the set and ratio of fatty acids (FA), which was represented by acids with chain lengths from C14 to C24. The bacterial biomass concentration and PHA synthesis were comparable (4.1–4.6 g/L and about 70%) when using WFO obtained from smoked-sprat heads and fresh mackerel, and it was twice as high as the bacterial biomass concentration from the fresh sprat waste. This depended on the type of WFO, the bacteria synthesized P(3HB) homopolymer or P(3HB-co-3HV-co-3HHx) copolymer, which had a lower degree of crystallinity (Cx 71%) and a lower molecular weight (Mn 134 kDa) compared to the P(3HB) (Mn 175–209 kDa and Cx 74–78%) at comparable temperatures (Tmelt and Tdegr of 158–168 °C and 261–284 °C, respectively). The new types of WFO, studied for the first time, are suitable as a carbon substrates for PHA synthesis. The WFOs obtained in the production of canned Baltic sprat and Baltic mackerel can be considered a promising and renewable substrate for PHA biosynthesis. |
| doi_str_mv | 10.3390/ijms241914919 |
| format | Article |
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All the WFOs ensured the growth of the wild-type strain Cupriavidus necator B-10646 and the synthesis of PHA, regardless of the degree of lipid saturation (from 0.52 to 0.65) and the set and ratio of fatty acids (FA), which was represented by acids with chain lengths from C14 to C24. The bacterial biomass concentration and PHA synthesis were comparable (4.1–4.6 g/L and about 70%) when using WFO obtained from smoked-sprat heads and fresh mackerel, and it was twice as high as the bacterial biomass concentration from the fresh sprat waste. This depended on the type of WFO, the bacteria synthesized P(3HB) homopolymer or P(3HB-co-3HV-co-3HHx) copolymer, which had a lower degree of crystallinity (Cx 71%) and a lower molecular weight (Mn 134 kDa) compared to the P(3HB) (Mn 175–209 kDa and Cx 74–78%) at comparable temperatures (Tmelt and Tdegr of 158–168 °C and 261–284 °C, respectively). The new types of WFO, studied for the first time, are suitable as a carbon substrates for PHA synthesis. The WFOs obtained in the production of canned Baltic sprat and Baltic mackerel can be considered a promising and renewable substrate for PHA biosynthesis.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms241914919</identifier><identifier>PMID: 37834364</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Analysis ; By products ; Canning industry ; Carbon ; Energy efficiency ; Fatty acids ; Fish oils ; Investigations ; Lipids ; Oils & fats ; Plastics ; Polyols ; Raw materials ; Soybeans ; Vegetable oils</subject><ispartof>International journal of molecular sciences, 2023-10, Vol.24 (19), p.14919</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. 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The WFOs obtained in the production of canned Baltic sprat and Baltic mackerel can be considered a promising and renewable substrate for PHA biosynthesis.</description><subject>Analysis</subject><subject>By products</subject><subject>Canning industry</subject><subject>Carbon</subject><subject>Energy efficiency</subject><subject>Fatty acids</subject><subject>Fish oils</subject><subject>Investigations</subject><subject>Lipids</subject><subject>Oils & fats</subject><subject>Plastics</subject><subject>Polyols</subject><subject>Raw materials</subject><subject>Soybeans</subject><subject>Vegetable oils</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpdkc9rFDEUx4MotlaP3gNe6mFqfs1kcpJSXSsUt1Cl4CUkmZfdrDOTNZltHf_6pmwRW3J4Ie_zvu8bvgi9peSEc0U-hM2QmaCKCkXVM3RIBWMVIY18_t_9AL3KeUMI46xWL9EBly0XvBGH6OdliltIU4CMo8efYJVMZ2wP-DL283ruUvwzm_6XGaOZCnM1j9MacvgLHfYpDvgb3OJrkyfAi5DXeBn6jI-vF8v8_jV64U2f4c1DPUI_Fp-_n51XF8svX89OLyonOJuqtm6JdOClMsQ5Zotf52XdEOsFt5wQ73jHqayZ5cZZZZS3xpLOs9aAMpQfoY973e3ODtA5GKdker1NYTBp1tEE_bgzhrVexRtNSS25qJuicPygkOLvHeRJDyE76HszQtxlzVopuaKM1gV99wTdxF0ay__uqUYK1ipZqJM9tTI96DD6WBa7cjoYgosj-FDeT6UsuREuRBmo9gMuxZwT-H_2KdH3OetHOfM71ROa6g</recordid><startdate>20231001</startdate><enddate>20231001</enddate><creator>Zhila, Natalia O.</creator><creator>Kiselev, Evgeniy G.</creator><creator>Volkov, Vladimir V.</creator><creator>Mezenova, Olga Ya</creator><creator>Sapozhnikova, Kristina Yu</creator><creator>Shishatskaya, Ekaterina I.</creator><creator>Volova, Tatiana G.</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7967-243X</orcidid><orcidid>https://orcid.org/0000-0003-4472-7087</orcidid><orcidid>https://orcid.org/0000-0001-6054-4200</orcidid></search><sort><creationdate>20231001</creationdate><title>Properties of Degradable Polyhydroxyalkanoates Synthesized from New Waste Fish Oils (WFOs)</title><author>Zhila, Natalia O. ; 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All the WFOs ensured the growth of the wild-type strain Cupriavidus necator B-10646 and the synthesis of PHA, regardless of the degree of lipid saturation (from 0.52 to 0.65) and the set and ratio of fatty acids (FA), which was represented by acids with chain lengths from C14 to C24. The bacterial biomass concentration and PHA synthesis were comparable (4.1–4.6 g/L and about 70%) when using WFO obtained from smoked-sprat heads and fresh mackerel, and it was twice as high as the bacterial biomass concentration from the fresh sprat waste. This depended on the type of WFO, the bacteria synthesized P(3HB) homopolymer or P(3HB-co-3HV-co-3HHx) copolymer, which had a lower degree of crystallinity (Cx 71%) and a lower molecular weight (Mn 134 kDa) compared to the P(3HB) (Mn 175–209 kDa and Cx 74–78%) at comparable temperatures (Tmelt and Tdegr of 158–168 °C and 261–284 °C, respectively). The new types of WFO, studied for the first time, are suitable as a carbon substrates for PHA synthesis. 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| subjects | Analysis By products Canning industry Carbon Energy efficiency Fatty acids Fish oils Investigations Lipids Oils & fats Plastics Polyols Raw materials Soybeans Vegetable oils |
| title | Properties of Degradable Polyhydroxyalkanoates Synthesized from New Waste Fish Oils (WFOs) |
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