Nitrate-induced changes in protein synthesis and translation of RNA in maize roots
Nitrate regulation of protein synthesis and RNA translation in maize (Zea mays L. var B73) roots was examined, using in vivo labeling with [35S]methionine and in vitro translation. Nitrate enhanced the synthesis of a 31 kilodalton membrane polypeptide which was localized in a fraction enriched in to...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1987-05, Vol.84 (1), p.52-57 |
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| creator | McClure, P.R Omholt, T.E Pace, G.M Bouthyette, P.Y |
| description | Nitrate regulation of protein synthesis and RNA translation in maize (Zea mays L. var B73) roots was examined, using in vivo labeling with [35S]methionine and in vitro translation. Nitrate enhanced the synthesis of a 31 kilodalton membrane polypeptide which was localized in a fraction enriched in tonoplast and/or endoplasmic reticulum membrane vesicles. The nitrate-enhanced synthesis was correlated with an acceleration of net nitrate uptake by seedlings during initial exposure to nitrate. Nitrate did not consistently enhance protein synthesis in other membrane fractions. Synthesis of up to four soluble polypeptides (21, 40, 90, and 168 kilodaltons) was also enhanced by nitrate. The most consistent enhancement was that of the 40 kilodalton polypeptide. No consistent nitrate-induced changes were noted in the organellar fraction (14,000g pellet of root homogenates). When roots were treated with nitrate, the amount of [35S]methionine increased in six in vitro translation products (21, 24, 41, 56, 66, and 90 kilodaltons). Nitrate treatment did not enhance accumulation of label in translation products with a molecular weight of 31,000 (corresponding to the identified nitrate-inducible membrane polypeptide). Incubation of in vitro translation products with root membranes caused changes in the SDS-PAGE profiles in the vicinity of 31 kilodaltons. The results suggest that the nitrate-inducible, 31 kilodalton polypeptide from a fraction enriched in tonoplast and/or endoplasmic reticulum may be involved in regulating nitrate accumulation by maize roots. |
| doi_str_mv | 10.1104/pp.84.1.52 |
| format | Article |
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Nitrate enhanced the synthesis of a 31 kilodalton membrane polypeptide which was localized in a fraction enriched in tonoplast and/or endoplasmic reticulum membrane vesicles. The nitrate-enhanced synthesis was correlated with an acceleration of net nitrate uptake by seedlings during initial exposure to nitrate. Nitrate did not consistently enhance protein synthesis in other membrane fractions. Synthesis of up to four soluble polypeptides (21, 40, 90, and 168 kilodaltons) was also enhanced by nitrate. The most consistent enhancement was that of the 40 kilodalton polypeptide. No consistent nitrate-induced changes were noted in the organellar fraction (14,000g pellet of root homogenates). When roots were treated with nitrate, the amount of [35S]methionine increased in six in vitro translation products (21, 24, 41, 56, 66, and 90 kilodaltons). Nitrate treatment did not enhance accumulation of label in translation products with a molecular weight of 31,000 (corresponding to the identified nitrate-inducible membrane polypeptide). Incubation of in vitro translation products with root membranes caused changes in the SDS-PAGE profiles in the vicinity of 31 kilodaltons. The results suggest that the nitrate-inducible, 31 kilodalton polypeptide from a fraction enriched in tonoplast and/or endoplasmic reticulum may be involved in regulating nitrate accumulation by maize roots.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.84.1.52</identifier><identifier>PMID: 16665404</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Agronomy. Soil science and plant productions ; ARN ; Biological and medical sciences ; Cell membranes ; Corn ; Economic plant physiology ; ESZEA MAYS ; Fundamental and applied biological sciences. Psychology ; Gene expression regulation ; Metabolism ; METHIO ; METHIONINE ; METIONINA ; Molecular Biology and Gene Regulation ; MOLECULAR WEIGHT ; NITRATE ; NITRATES ; NITRATOS ; Nitrogen metabolism ; Nitrogen metabolism and other ones (excepting carbon metabolism) ; Nutrition. Photosynthesis. Respiration. Metabolism ; PEPTIDE ; PEPTIDES ; PEPTIDOS ; PESO MOLECULAR ; Plant physiology and development ; Plant roots ; Plants ; POIDS MOLECULAIRE ; Product labeling ; PROTEIN SYNTHESIS ; RACINE ; RAICES ; RNA ; ROOTS ; Seedlings ; SINTESIS DE PROTEINAS ; SYNTHESE PROTEIQUE ; Tonoplast ; ZEA MAYS</subject><ispartof>Plant physiology (Bethesda), 1987-05, Vol.84 (1), p.52-57</ispartof><rights>Copyright 1987 American Society of Plant Physiologists</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-8bd89a472dd0b4f8505acb9404a573e8688c2f48478180c200c1e0ae4e5fcd73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4270576$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4270576$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7513940$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16665404$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McClure, P.R</creatorcontrib><creatorcontrib>Omholt, T.E</creatorcontrib><creatorcontrib>Pace, G.M</creatorcontrib><creatorcontrib>Bouthyette, P.Y</creatorcontrib><title>Nitrate-induced changes in protein synthesis and translation of RNA in maize roots</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Nitrate regulation of protein synthesis and RNA translation in maize (Zea mays L. var B73) roots was examined, using in vivo labeling with [35S]methionine and in vitro translation. Nitrate enhanced the synthesis of a 31 kilodalton membrane polypeptide which was localized in a fraction enriched in tonoplast and/or endoplasmic reticulum membrane vesicles. The nitrate-enhanced synthesis was correlated with an acceleration of net nitrate uptake by seedlings during initial exposure to nitrate. Nitrate did not consistently enhance protein synthesis in other membrane fractions. Synthesis of up to four soluble polypeptides (21, 40, 90, and 168 kilodaltons) was also enhanced by nitrate. The most consistent enhancement was that of the 40 kilodalton polypeptide. No consistent nitrate-induced changes were noted in the organellar fraction (14,000g pellet of root homogenates). When roots were treated with nitrate, the amount of [35S]methionine increased in six in vitro translation products (21, 24, 41, 56, 66, and 90 kilodaltons). Nitrate treatment did not enhance accumulation of label in translation products with a molecular weight of 31,000 (corresponding to the identified nitrate-inducible membrane polypeptide). Incubation of in vitro translation products with root membranes caused changes in the SDS-PAGE profiles in the vicinity of 31 kilodaltons. The results suggest that the nitrate-inducible, 31 kilodalton polypeptide from a fraction enriched in tonoplast and/or endoplasmic reticulum may be involved in regulating nitrate accumulation by maize roots.</description><subject>Agronomy. Soil science and plant productions</subject><subject>ARN</subject><subject>Biological and medical sciences</subject><subject>Cell membranes</subject><subject>Corn</subject><subject>Economic plant physiology</subject><subject>ESZEA MAYS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression regulation</subject><subject>Metabolism</subject><subject>METHIO</subject><subject>METHIONINE</subject><subject>METIONINA</subject><subject>Molecular Biology and Gene Regulation</subject><subject>MOLECULAR WEIGHT</subject><subject>NITRATE</subject><subject>NITRATES</subject><subject>NITRATOS</subject><subject>Nitrogen metabolism</subject><subject>Nitrogen metabolism and other ones (excepting carbon metabolism)</subject><subject>Nutrition. Photosynthesis. Respiration. Metabolism</subject><subject>PEPTIDE</subject><subject>PEPTIDES</subject><subject>PEPTIDOS</subject><subject>PESO MOLECULAR</subject><subject>Plant physiology and development</subject><subject>Plant roots</subject><subject>Plants</subject><subject>POIDS MOLECULAIRE</subject><subject>Product labeling</subject><subject>PROTEIN SYNTHESIS</subject><subject>RACINE</subject><subject>RAICES</subject><subject>RNA</subject><subject>ROOTS</subject><subject>Seedlings</subject><subject>SINTESIS DE PROTEINAS</subject><subject>SYNTHESE PROTEIQUE</subject><subject>Tonoplast</subject><subject>ZEA MAYS</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNpVkVFLHDEUhUOx1NX2pc-lzIMgCLO9ySSTOy-CiNqCWLD2OWQzmd3IbDImWcH--kZ2Wdune-F8nHs4l5DPFOaUAv82TXPkczoX7B2ZUdGwmgmOB2QGUHZA7A7JUUqPAEAbyj-QQ9q2reDAZ-T-zuWos62d7zfG9pVZab-0qXK-mmLItsz04vPKJpcq7fuq4D6NOrvgqzBU93cXr-xauz-2iiHk9JG8H_SY7KfdPCYP11cPl9_r2583Py4vbmvDKcs1LnrsNJes72HBBxQgtFl0JZUWsrHYIho2cOQSKYJhAIZa0JZbMZheNsfkfGs7bRZr2xvrS7JRTdGtdXxRQTv1v-LdSi3Ds6IgWsHaYnC6M4jhaWNTVmuXjB1H7W3YJCWbhnel4K6QZ1vSxJBStMP-CgX1-gI1TQq5okqwAn_9N9cbuuu8ACc7QCejx6H0aVzac1LQprRQsC9b7DHlEPcyZxKEbN_kQQell7E4_P6FCJ1sGWLzFzVQoWs</recordid><startdate>19870501</startdate><enddate>19870501</enddate><creator>McClure, P.R</creator><creator>Omholt, T.E</creator><creator>Pace, G.M</creator><creator>Bouthyette, P.Y</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19870501</creationdate><title>Nitrate-induced changes in protein synthesis and translation of RNA in maize roots</title><author>McClure, P.R ; Omholt, T.E ; Pace, G.M ; Bouthyette, P.Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-8bd89a472dd0b4f8505acb9404a573e8688c2f48478180c200c1e0ae4e5fcd73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>ARN</topic><topic>Biological and medical sciences</topic><topic>Cell membranes</topic><topic>Corn</topic><topic>Economic plant physiology</topic><topic>ESZEA MAYS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression regulation</topic><topic>Metabolism</topic><topic>METHIO</topic><topic>METHIONINE</topic><topic>METIONINA</topic><topic>Molecular Biology and Gene Regulation</topic><topic>MOLECULAR WEIGHT</topic><topic>NITRATE</topic><topic>NITRATES</topic><topic>NITRATOS</topic><topic>Nitrogen metabolism</topic><topic>Nitrogen metabolism and other ones (excepting carbon metabolism)</topic><topic>Nutrition. Photosynthesis. Respiration. Metabolism</topic><topic>PEPTIDE</topic><topic>PEPTIDES</topic><topic>PEPTIDOS</topic><topic>PESO MOLECULAR</topic><topic>Plant physiology and development</topic><topic>Plant roots</topic><topic>Plants</topic><topic>POIDS MOLECULAIRE</topic><topic>Product labeling</topic><topic>PROTEIN SYNTHESIS</topic><topic>RACINE</topic><topic>RAICES</topic><topic>RNA</topic><topic>ROOTS</topic><topic>Seedlings</topic><topic>SINTESIS DE PROTEINAS</topic><topic>SYNTHESE PROTEIQUE</topic><topic>Tonoplast</topic><topic>ZEA MAYS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McClure, P.R</creatorcontrib><creatorcontrib>Omholt, T.E</creatorcontrib><creatorcontrib>Pace, G.M</creatorcontrib><creatorcontrib>Bouthyette, P.Y</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McClure, P.R</au><au>Omholt, T.E</au><au>Pace, G.M</au><au>Bouthyette, P.Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nitrate-induced changes in protein synthesis and translation of RNA in maize roots</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1987-05-01</date><risdate>1987</risdate><volume>84</volume><issue>1</issue><spage>52</spage><epage>57</epage><pages>52-57</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Nitrate regulation of protein synthesis and RNA translation in maize (Zea mays L. var B73) roots was examined, using in vivo labeling with [35S]methionine and in vitro translation. Nitrate enhanced the synthesis of a 31 kilodalton membrane polypeptide which was localized in a fraction enriched in tonoplast and/or endoplasmic reticulum membrane vesicles. The nitrate-enhanced synthesis was correlated with an acceleration of net nitrate uptake by seedlings during initial exposure to nitrate. Nitrate did not consistently enhance protein synthesis in other membrane fractions. Synthesis of up to four soluble polypeptides (21, 40, 90, and 168 kilodaltons) was also enhanced by nitrate. The most consistent enhancement was that of the 40 kilodalton polypeptide. No consistent nitrate-induced changes were noted in the organellar fraction (14,000g pellet of root homogenates). When roots were treated with nitrate, the amount of [35S]methionine increased in six in vitro translation products (21, 24, 41, 56, 66, and 90 kilodaltons). Nitrate treatment did not enhance accumulation of label in translation products with a molecular weight of 31,000 (corresponding to the identified nitrate-inducible membrane polypeptide). Incubation of in vitro translation products with root membranes caused changes in the SDS-PAGE profiles in the vicinity of 31 kilodaltons. The results suggest that the nitrate-inducible, 31 kilodalton polypeptide from a fraction enriched in tonoplast and/or endoplasmic reticulum may be involved in regulating nitrate accumulation by maize roots.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16665404</pmid><doi>10.1104/pp.84.1.52</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1987-05, Vol.84 (1), p.52-57 |
| issn | 0032-0889 1532-2548 |
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| source | Jstor Complete Legacy; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Agronomy. Soil science and plant productions ARN Biological and medical sciences Cell membranes Corn Economic plant physiology ESZEA MAYS Fundamental and applied biological sciences. Psychology Gene expression regulation Metabolism METHIO METHIONINE METIONINA Molecular Biology and Gene Regulation MOLECULAR WEIGHT NITRATE NITRATES NITRATOS Nitrogen metabolism Nitrogen metabolism and other ones (excepting carbon metabolism) Nutrition. Photosynthesis. Respiration. Metabolism PEPTIDE PEPTIDES PEPTIDOS PESO MOLECULAR Plant physiology and development Plant roots Plants POIDS MOLECULAIRE Product labeling PROTEIN SYNTHESIS RACINE RAICES RNA ROOTS Seedlings SINTESIS DE PROTEINAS SYNTHESE PROTEIQUE Tonoplast ZEA MAYS |
| title | Nitrate-induced changes in protein synthesis and translation of RNA in maize roots |
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