Selective solubilization of membrane proteins differentially labeled by p-chloromercuribenzenesulfonic acid in the presence of sucrose

Broadbean (Vicia faba L.) leaf discs have been incubated with the slowly permeant thiol reagent [203Hg]-para-chloromercuribenzenesulfonic acid (PCMBS) in the presence or in the absence of sucrose, and the release of PCMBS-labeled proteins has been monitored in media containing various concentrations...

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Veröffentlicht in:	Plant Physiol.; (United States) 1987-03, Vol.83 (3), p.541-545
Hauptverfasser:	M'Batchi, B, Pichelin, D, Delrot, S
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques Absorption. Translocation of ions and substances. Permeability ADDITIVES Agronomy. Soil science and plant productions BACTERIA BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences CARBOHYDRATES CELL CONSTITUENTS CELL MEMBRANES Cell physiology DAYS LIVING RADIOISOTOPES DETERGENTS DIFFERENTIAL SOLUBILIZATION DISACCHARIDES Economic plant physiology EMULSIFIERS ETIQUETADO EVEN-ODD NUCLEI FEUILLE Fundamental and applied biological sciences. Psychology Gels HEAVY NUCLEI HOJAS ISOTOPE APPLICATIONS ISOTOPES LABELLING LEAVES LEGUMINOSAE MARQUAGE Membrane and intracellular transports Membrane proteins MEMBRANE TRANSPORT MEMBRANES Membranes and Bioenergetics MERCURY 203 MERCURY ISOTOPES MICROORGANISMS Molecular and cellular biology NUCLEI Nutrition. Photosynthesis. Respiration. Metabolism OLIGOSACCHARIDES ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC SULFUR COMPOUNDS PLANTS PROTEINAS PROTEINE PROTEINS Radioactive decay RADIOISOTOPES Reagents RESPONSE MODIFYING FACTORS RHIZOBIUM SACCHARIDES SACCHAROSE Sodium SOLUBILIDAD SOLUBILITE SOLUBILITY Solubilization SUCROSA SUCROSE Sugars SULFONIC ACIDS SURFACE ACTIVE AGENTS SURFACTANT SURFACTANTES SURFACTANTS THIOL THIOLS TIOLES TRACER TECHNIQUES VICIA VICIA FABA WETTING AGENTS
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