Male germ unit isolation from three tricellular pollen species: Brassica oleracea, Zea mays, and Triticum aestivum

A technical procedure is described to follow the in vitro release of the 'male germ unit' and the sperm cells from three tricellular pollen species (Brassica, Zea, and Triticum). The condition of the sperm cell was controlled using light microscopy. In addition, for the first time, the spe...

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Veröffentlicht in:	Plant physiology (Bethesda) 1987-03, Vol.83 (3), p.464-466
Hauptverfasser:	Matthys-Rochon, E, Vergne, P, Detchepare, S, Dumas, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions AISLAMIENTO (TECNICA) Animal cells Biological and medical sciences BRASSICA OLERACEA Cell fusion Cell nucleus Cell separation CELLS CELLULE CELULAS Communications Development and Growth Regulation Economic plant physiology EPURATION Fertilization Fundamental and applied biological sciences. Psychology ISOLATION ISOLEMENT MET METHODS METODOS Morphology. Anatomy. Histology. Cytology Plant cells Plant physiology and development Plants POLEN POLLEN PURIFICACION PURIFICATION Spermatozoa Tissue cultures, protoplasts TRITICUM AESTIVUM VIABILIDAD VIABILITE VIABILITY ZEA MA0540YS ZEA MAYS
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