Mineral nutrient requirements of a loblolly pine (Pinus taeda) cell suspension culture. Evaluation of a medium formulated from seed composition data
The mineral nutrient requirements of Pinus taeda cells were explored using quantitative cell culture growth measurements. An appraisal was thereby made of the critical features of a novel and successful medium which was developed specifically for this gymnosperm using chemical composition data for d...
Gespeichert in:
| Veröffentlicht in: | Plant physiology (Bethesda) 1986-12, Vol.82 (4), p.942-945 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 945 |
|---|---|
| container_issue | 4 |
| container_start_page | 942 |
| container_title | Plant physiology (Bethesda) |
| container_volume | 82 |
| creator | Teasdale, R.D Dawson, P.A Woolhouse, H.W |
| description | The mineral nutrient requirements of Pinus taeda cells were explored using quantitative cell culture growth measurements. An appraisal was thereby made of the critical features of a novel and successful medium which was developed specifically for this gymnosperm using chemical composition data for developing seeds, and characterized by generally high concentration of all micronutrients, high magnesium, and low calcium. The high magnesium concentration was found not to be detrimental and possibly beneficial whereas the calcium level bordered on a deficiency threshold. Within the microelements high iodide was found to be essential, as was a higher borate level than is present in media developed for angiosperms. High zinc concentrations were also beneficial, with normal levels permitting slower but nevertheless healthy growth. An improved medium was thereby formulated which was stress-free and exhibited broader genotype specificity. This new formulation has proved very successful in maintaining long-term growth of highly uniform and apparently meristematic suspension cultures of Pinus radiata. |
| doi_str_mv | 10.1104/pp.82.4.942 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1056237</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4270303</jstor_id><sourcerecordid>4270303</sourcerecordid><originalsourceid>FETCH-LOGICAL-c308t-5d9086cdaa83c95de9a34a1b04dd5d19539e44848f92c17bcc77627045750fcd3</originalsourceid><addsrcrecordid>eNpVkV1rFDEUhoModq1eeSuSOxXZNZkkk8xNQUr9gIqC9jqcTTI1JTOZ5qPQ_-EPNttdWr3KIefhPe85L0IvKdlQSviHZdmobsM3A-8eoRUVrFt3gqvHaEVIq4lSwxF6lvMVIYQyyp-iI9r3vaCSrNCfb352CQKea0nezQUnd119clOrM44jBhziNsQQbvHSWPz2h59rxgWchXfYuBBwrnlxc_ZxxqaGUpPb4LMbCBXK7u9OZHLW1wmPMU01QHEWjylOOLtWmTgtMfs72EKB5-jJCCG7F4f3GF18Ovt1-mV9_v3z19OP52vDiCprYQeiemMBFDODsG4AxoFuCbdWWDoINjjOFVfj0Bkqt8ZI2XeScCEFGY1lx-hkr7vUbfNn2srtFHpJfoJ0qyN4_X9n9r_1ZbzRlIi-Y7IJvDkIpHhdXS568nl3EphdrFlLxvhAJO0a-X5PmhRzTm68n0KJ3sWol0WrTnPdYmz063-NPbCH3Brwag9c5RLTfZ-37RhhD-0RoobL5LO--KmkEM2QUuwvzYGumw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733490712</pqid></control><display><type>article</type><title>Mineral nutrient requirements of a loblolly pine (Pinus taeda) cell suspension culture. Evaluation of a medium formulated from seed composition data</title><source>Jstor Complete Legacy</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Teasdale, R.D ; Dawson, P.A ; Woolhouse, H.W</creator><creatorcontrib>Teasdale, R.D ; Dawson, P.A ; Woolhouse, H.W</creatorcontrib><description>The mineral nutrient requirements of Pinus taeda cells were explored using quantitative cell culture growth measurements. An appraisal was thereby made of the critical features of a novel and successful medium which was developed specifically for this gymnosperm using chemical composition data for developing seeds, and characterized by generally high concentration of all micronutrients, high magnesium, and low calcium. The high magnesium concentration was found not to be detrimental and possibly beneficial whereas the calcium level bordered on a deficiency threshold. Within the microelements high iodide was found to be essential, as was a higher borate level than is present in media developed for angiosperms. High zinc concentrations were also beneficial, with normal levels permitting slower but nevertheless healthy growth. An improved medium was thereby formulated which was stress-free and exhibited broader genotype specificity. This new formulation has proved very successful in maintaining long-term growth of highly uniform and apparently meristematic suspension cultures of Pinus radiata.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.82.4.942</identifier><identifier>PMID: 16665170</identifier><language>eng</language><publisher>United States: American Society of Plant Physiologists</publisher><subject>ANALISIS ; ANALYSE ; ANALYSIS ; BESOIN NU0467TRITIONNEL ; Borates ; CELL CULTURE ; Cell culture techniques ; Cell growth ; Chemical suspensions ; Chlorides ; CULTIVO DE CELULAS ; CULTIVO DE TEJIDOS ; CULTURE DE CELLULES ; CULTURE DE TISSUS ; Cultured cells ; GRAINE ; GROWING MEDIA ; Iodides ; MEDIO DE CULTIVO ; MINERALES ; MINERALS ; MINERAUX ; NECESIDADES DE NUTRIENTES ; NUTRICION ; NUTRITION ; Nutritional deficiencies ; NUTRITIONAL REQUIREMENTS ; OLIGOELE ; OLIGOELEMENTOS ; Phosphates ; PINUS RADIATA ; PINUS TAEDA ; SEEDS ; SEMILLA ; SUBSTRAT DE CULTURE ; TISSUE CULTURE ; TRACE ELEMENTS</subject><ispartof>Plant physiology (Bethesda), 1986-12, Vol.82 (4), p.942-945</ispartof><rights>Copyright 1986 American Society of Plant Physiologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4270303$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4270303$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,777,781,800,882,27905,27906,57998,58231</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16665170$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teasdale, R.D</creatorcontrib><creatorcontrib>Dawson, P.A</creatorcontrib><creatorcontrib>Woolhouse, H.W</creatorcontrib><title>Mineral nutrient requirements of a loblolly pine (Pinus taeda) cell suspension culture. Evaluation of a medium formulated from seed composition data</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The mineral nutrient requirements of Pinus taeda cells were explored using quantitative cell culture growth measurements. An appraisal was thereby made of the critical features of a novel and successful medium which was developed specifically for this gymnosperm using chemical composition data for developing seeds, and characterized by generally high concentration of all micronutrients, high magnesium, and low calcium. The high magnesium concentration was found not to be detrimental and possibly beneficial whereas the calcium level bordered on a deficiency threshold. Within the microelements high iodide was found to be essential, as was a higher borate level than is present in media developed for angiosperms. High zinc concentrations were also beneficial, with normal levels permitting slower but nevertheless healthy growth. An improved medium was thereby formulated which was stress-free and exhibited broader genotype specificity. This new formulation has proved very successful in maintaining long-term growth of highly uniform and apparently meristematic suspension cultures of Pinus radiata.</description><subject>ANALISIS</subject><subject>ANALYSE</subject><subject>ANALYSIS</subject><subject>BESOIN NU0467TRITIONNEL</subject><subject>Borates</subject><subject>CELL CULTURE</subject><subject>Cell culture techniques</subject><subject>Cell growth</subject><subject>Chemical suspensions</subject><subject>Chlorides</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTIVO DE TEJIDOS</subject><subject>CULTURE DE CELLULES</subject><subject>CULTURE DE TISSUS</subject><subject>Cultured cells</subject><subject>GRAINE</subject><subject>GROWING MEDIA</subject><subject>Iodides</subject><subject>MEDIO DE CULTIVO</subject><subject>MINERALES</subject><subject>MINERALS</subject><subject>MINERAUX</subject><subject>NECESIDADES DE NUTRIENTES</subject><subject>NUTRICION</subject><subject>NUTRITION</subject><subject>Nutritional deficiencies</subject><subject>NUTRITIONAL REQUIREMENTS</subject><subject>OLIGOELE</subject><subject>OLIGOELEMENTOS</subject><subject>Phosphates</subject><subject>PINUS RADIATA</subject><subject>PINUS TAEDA</subject><subject>SEEDS</subject><subject>SEMILLA</subject><subject>SUBSTRAT DE CULTURE</subject><subject>TISSUE CULTURE</subject><subject>TRACE ELEMENTS</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNpVkV1rFDEUhoModq1eeSuSOxXZNZkkk8xNQUr9gIqC9jqcTTI1JTOZ5qPQ_-EPNttdWr3KIefhPe85L0IvKdlQSviHZdmobsM3A-8eoRUVrFt3gqvHaEVIq4lSwxF6lvMVIYQyyp-iI9r3vaCSrNCfb352CQKea0nezQUnd119clOrM44jBhziNsQQbvHSWPz2h59rxgWchXfYuBBwrnlxc_ZxxqaGUpPb4LMbCBXK7u9OZHLW1wmPMU01QHEWjylOOLtWmTgtMfs72EKB5-jJCCG7F4f3GF18Ovt1-mV9_v3z19OP52vDiCprYQeiemMBFDODsG4AxoFuCbdWWDoINjjOFVfj0Bkqt8ZI2XeScCEFGY1lx-hkr7vUbfNn2srtFHpJfoJ0qyN4_X9n9r_1ZbzRlIi-Y7IJvDkIpHhdXS568nl3EphdrFlLxvhAJO0a-X5PmhRzTm68n0KJ3sWol0WrTnPdYmz063-NPbCH3Brwag9c5RLTfZ-37RhhD-0RoobL5LO--KmkEM2QUuwvzYGumw</recordid><startdate>198612</startdate><enddate>198612</enddate><creator>Teasdale, R.D</creator><creator>Dawson, P.A</creator><creator>Woolhouse, H.W</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>198612</creationdate><title>Mineral nutrient requirements of a loblolly pine (Pinus taeda) cell suspension culture. Evaluation of a medium formulated from seed composition data</title><author>Teasdale, R.D ; Dawson, P.A ; Woolhouse, H.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c308t-5d9086cdaa83c95de9a34a1b04dd5d19539e44848f92c17bcc77627045750fcd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>ANALISIS</topic><topic>ANALYSE</topic><topic>ANALYSIS</topic><topic>BESOIN NU0467TRITIONNEL</topic><topic>Borates</topic><topic>CELL CULTURE</topic><topic>Cell culture techniques</topic><topic>Cell growth</topic><topic>Chemical suspensions</topic><topic>Chlorides</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTIVO DE TEJIDOS</topic><topic>CULTURE DE CELLULES</topic><topic>CULTURE DE TISSUS</topic><topic>Cultured cells</topic><topic>GRAINE</topic><topic>GROWING MEDIA</topic><topic>Iodides</topic><topic>MEDIO DE CULTIVO</topic><topic>MINERALES</topic><topic>MINERALS</topic><topic>MINERAUX</topic><topic>NECESIDADES DE NUTRIENTES</topic><topic>NUTRICION</topic><topic>NUTRITION</topic><topic>Nutritional deficiencies</topic><topic>NUTRITIONAL REQUIREMENTS</topic><topic>OLIGOELE</topic><topic>OLIGOELEMENTOS</topic><topic>Phosphates</topic><topic>PINUS RADIATA</topic><topic>PINUS TAEDA</topic><topic>SEEDS</topic><topic>SEMILLA</topic><topic>SUBSTRAT DE CULTURE</topic><topic>TISSUE CULTURE</topic><topic>TRACE ELEMENTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teasdale, R.D</creatorcontrib><creatorcontrib>Dawson, P.A</creatorcontrib><creatorcontrib>Woolhouse, H.W</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teasdale, R.D</au><au>Dawson, P.A</au><au>Woolhouse, H.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mineral nutrient requirements of a loblolly pine (Pinus taeda) cell suspension culture. Evaluation of a medium formulated from seed composition data</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1986-12</date><risdate>1986</risdate><volume>82</volume><issue>4</issue><spage>942</spage><epage>945</epage><pages>942-945</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>The mineral nutrient requirements of Pinus taeda cells were explored using quantitative cell culture growth measurements. An appraisal was thereby made of the critical features of a novel and successful medium which was developed specifically for this gymnosperm using chemical composition data for developing seeds, and characterized by generally high concentration of all micronutrients, high magnesium, and low calcium. The high magnesium concentration was found not to be detrimental and possibly beneficial whereas the calcium level bordered on a deficiency threshold. Within the microelements high iodide was found to be essential, as was a higher borate level than is present in media developed for angiosperms. High zinc concentrations were also beneficial, with normal levels permitting slower but nevertheless healthy growth. An improved medium was thereby formulated which was stress-free and exhibited broader genotype specificity. This new formulation has proved very successful in maintaining long-term growth of highly uniform and apparently meristematic suspension cultures of Pinus radiata.</abstract><cop>United States</cop><pub>American Society of Plant Physiologists</pub><pmid>16665170</pmid><doi>10.1104/pp.82.4.942</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1986-12, Vol.82 (4), p.942-945 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1056237 |
| source | Jstor Complete Legacy; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | ANALISIS ANALYSE ANALYSIS BESOIN NU0467TRITIONNEL Borates CELL CULTURE Cell culture techniques Cell growth Chemical suspensions Chlorides CULTIVO DE CELULAS CULTIVO DE TEJIDOS CULTURE DE CELLULES CULTURE DE TISSUS Cultured cells GRAINE GROWING MEDIA Iodides MEDIO DE CULTIVO MINERALES MINERALS MINERAUX NECESIDADES DE NUTRIENTES NUTRICION NUTRITION Nutritional deficiencies NUTRITIONAL REQUIREMENTS OLIGOELE OLIGOELEMENTOS Phosphates PINUS RADIATA PINUS TAEDA SEEDS SEMILLA SUBSTRAT DE CULTURE TISSUE CULTURE TRACE ELEMENTS |
| title | Mineral nutrient requirements of a loblolly pine (Pinus taeda) cell suspension culture. Evaluation of a medium formulated from seed composition data |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T18%3A58%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mineral%20nutrient%20requirements%20of%20a%20loblolly%20pine%20(Pinus%20taeda)%20cell%20suspension%20culture.%20Evaluation%20of%20a%20medium%20formulated%20from%20seed%20composition%20data&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Teasdale,%20R.D&rft.date=1986-12&rft.volume=82&rft.issue=4&rft.spage=942&rft.epage=945&rft.pages=942-945&rft.issn=0032-0889&rft.eissn=1532-2548&rft_id=info:doi/10.1104/pp.82.4.942&rft_dat=%3Cjstor_pubme%3E4270303%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733490712&rft_id=info:pmid/16665170&rft_jstor_id=4270303&rfr_iscdi=true |