Characterization of the hexose transport system in maize root tips
Sugar-depleted excised maize (Zea mays L.) root tips were used to study the kinetics and the specificity of hexose uptake. It was found that difficulties induced by bulk diffusion and penetration barriers did not exist with root tips. Several lines of evidence indicate the existence of a complex set...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1988-12, Vol.88 (4), p.1015-1020 |
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| container_title | Plant physiology (Bethesda) |
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| creator | Xia, J.H Saglio, P.H |
| description | Sugar-depleted excised maize (Zea mays L.) root tips were used to study the kinetics and the specificity of hexose uptake. It was found that difficulties induced by bulk diffusion and penetration barriers did not exist with root tips. Several lines of evidence indicate the existence of a complex set of uptake systems for hexoses showing an overall biphasic dependence on external sugar concentrations. The results suggest that the high and the low affinity components might be located on the same carrier. One uptake system was specific for fructose, but the high affinity component was repressed by high concentrations of external glucose. A second system was specific for glucose and its analogs (2-deoxy-D-glucose and 3-O-methyl-D-glucose), and a third one, more complex, had a high affinity for glucose and its analogs but could transport fructose when glucose was not present in the external solution. A simple method is proposed to determine the inhibitor constants in competition experiments. |
| doi_str_mv | 10.1104/pp.88.4.1015 |
| format | Article |
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It was found that difficulties induced by bulk diffusion and penetration barriers did not exist with root tips. Several lines of evidence indicate the existence of a complex set of uptake systems for hexoses showing an overall biphasic dependence on external sugar concentrations. The results suggest that the high and the low affinity components might be located on the same carrier. One uptake system was specific for fructose, but the high affinity component was repressed by high concentrations of external glucose. A second system was specific for glucose and its analogs (2-deoxy-D-glucose and 3-O-methyl-D-glucose), and a third one, more complex, had a high affinity for glucose and its analogs but could transport fructose when glucose was not present in the external solution. A simple method is proposed to determine the inhibitor constants in competition experiments.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.88.4.1015</identifier><identifier>PMID: 16666413</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>AZUCARES ; Binding sites ; Biological and medical sciences ; Corn ; Fundamental and applied biological sciences. Psychology ; Genetics ; Hexoses ; Kinetics ; Life Sciences ; Mathematical constants ; Membranes and Bioenergetics ; Plant physiology and development ; Plants ; Plants genetics ; Protoplasts ; RACINE ; RAICES ; Root tips ; ROOTS ; SUCRES ; SUGARS ; TRANSLOCACION ; TRANSLOCATION ; Velocity ; Water and solutes. Absorption, translocation and permeability ; ZEA MAYS</subject><ispartof>Plant physiology (Bethesda), 1988-12, Vol.88 (4), p.1015-1020</ispartof><rights>Copyright 1988 The American Society of Plant Physiologists</rights><rights>1990 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-7493a5c3f6aa2ba09349efe987769d8d781482451f425cfe530be21f0f8c128c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4271702$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4271702$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6594027$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16666413$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02725177$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Xia, J.H</creatorcontrib><creatorcontrib>Saglio, P.H</creatorcontrib><title>Characterization of the hexose transport system in maize root tips</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Sugar-depleted excised maize (Zea mays L.) root tips were used to study the kinetics and the specificity of hexose uptake. It was found that difficulties induced by bulk diffusion and penetration barriers did not exist with root tips. Several lines of evidence indicate the existence of a complex set of uptake systems for hexoses showing an overall biphasic dependence on external sugar concentrations. The results suggest that the high and the low affinity components might be located on the same carrier. One uptake system was specific for fructose, but the high affinity component was repressed by high concentrations of external glucose. A second system was specific for glucose and its analogs (2-deoxy-D-glucose and 3-O-methyl-D-glucose), and a third one, more complex, had a high affinity for glucose and its analogs but could transport fructose when glucose was not present in the external solution. A simple method is proposed to determine the inhibitor constants in competition experiments.</description><subject>AZUCARES</subject><subject>Binding sites</subject><subject>Biological and medical sciences</subject><subject>Corn</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics</subject><subject>Hexoses</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>Mathematical constants</subject><subject>Membranes and Bioenergetics</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Plants genetics</subject><subject>Protoplasts</subject><subject>RACINE</subject><subject>RAICES</subject><subject>Root tips</subject><subject>ROOTS</subject><subject>SUCRES</subject><subject>SUGARS</subject><subject>TRANSLOCACION</subject><subject>TRANSLOCATION</subject><subject>Velocity</subject><subject>Water and solutes. Absorption, translocation and permeability</subject><subject>ZEA MAYS</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNpdks9rFDEcxYModq3ePInIHAQRumu--bFJLkJdtBUWPGjPIZtNOikzkzHJFtu_vllmWbW5JOR98vLCC0KvAS8AMPs0jgspF2wBGPgTNANOyZxwJp-iGcZ1jaVUJ-hFzjcYY6DAnqMTWNbBgM7Ql1VrkrHFpXBvSohDE31TWte07k_MrinJDHmMqTT5LhfXN2FoehPuXZNiLE0JY36JnnnTZffqMJ-iq29ff60u5-sfF99X5-u5ZZyUuWCKGm6pXxpDNgYrypTzTkkhlmort0ICk4Rx8Ixw6x2neOMIeOylBSItPUWfJ99xt-nd1rqhhuv0mEJv0p2OJuj_lSG0-jreasCcCyyqwcfJoH107PJ8rfd7mAjCQYhbqOyHw2Up_t65XHQfsnVdZwYXd1kLWuMTCaqSZxNpU8w5OX-0Bqz3Delx1FJqpvcNVfzdv6_4Cx8qqcD7A2CyNZ2vBdiQj9ySK1ZzVuzthN3kEtNRZkSAwKTKbybZm6jNdaoOVz-lqh9ASfoA9mOqLg</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>Xia, J.H</creator><creator>Saglio, P.H</creator><general>American Society of Plant Physiologists</general><general>Oxford University Press ; American Society of Plant Biologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope></search><sort><creationdate>19881201</creationdate><title>Characterization of the hexose transport system in maize root tips</title><author>Xia, J.H ; Saglio, P.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-7493a5c3f6aa2ba09349efe987769d8d781482451f425cfe530be21f0f8c128c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>AZUCARES</topic><topic>Binding sites</topic><topic>Biological and medical sciences</topic><topic>Corn</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics</topic><topic>Hexoses</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>Mathematical constants</topic><topic>Membranes and Bioenergetics</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Plants genetics</topic><topic>Protoplasts</topic><topic>RACINE</topic><topic>RAICES</topic><topic>Root tips</topic><topic>ROOTS</topic><topic>SUCRES</topic><topic>SUGARS</topic><topic>TRANSLOCACION</topic><topic>TRANSLOCATION</topic><topic>Velocity</topic><topic>Water and solutes. Absorption, translocation and permeability</topic><topic>ZEA MAYS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xia, J.H</creatorcontrib><creatorcontrib>Saglio, P.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xia, J.H</au><au>Saglio, P.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the hexose transport system in maize root tips</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>88</volume><issue>4</issue><spage>1015</spage><epage>1020</epage><pages>1015-1020</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Sugar-depleted excised maize (Zea mays L.) root tips were used to study the kinetics and the specificity of hexose uptake. It was found that difficulties induced by bulk diffusion and penetration barriers did not exist with root tips. Several lines of evidence indicate the existence of a complex set of uptake systems for hexoses showing an overall biphasic dependence on external sugar concentrations. The results suggest that the high and the low affinity components might be located on the same carrier. One uptake system was specific for fructose, but the high affinity component was repressed by high concentrations of external glucose. A second system was specific for glucose and its analogs (2-deoxy-D-glucose and 3-O-methyl-D-glucose), and a third one, more complex, had a high affinity for glucose and its analogs but could transport fructose when glucose was not present in the external solution. A simple method is proposed to determine the inhibitor constants in competition experiments.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16666413</pmid><doi>10.1104/pp.88.4.1015</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1988-12, Vol.88 (4), p.1015-1020 |
| issn | 0032-0889 1532-2548 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing; Alma/SFX Local Collection |
| subjects | AZUCARES Binding sites Biological and medical sciences Corn Fundamental and applied biological sciences. Psychology Genetics Hexoses Kinetics Life Sciences Mathematical constants Membranes and Bioenergetics Plant physiology and development Plants Plants genetics Protoplasts RACINE RAICES Root tips ROOTS SUCRES SUGARS TRANSLOCACION TRANSLOCATION Velocity Water and solutes. Absorption, translocation and permeability ZEA MAYS |
| title | Characterization of the hexose transport system in maize root tips |
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