Transformation of soybean cells using mixed strains of Agrobacterium tumefaciens and phenolic compounds
Cotyledon explants from germinated 1-day-old soybean seedling were inoculated with single or mixed strains of Agrobacterium tumefaciens. Mixed-strain infections with the supervirulent L,L-succinamopine type strain A281 (pTiBo542) and strain LBA4404 carrying an octopine type virulence (vir) region an...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1988-11, Vol.88 (3), p.570-573 |
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| creator | Owens, L.D Smigocki, A.C |
| description | Cotyledon explants from germinated 1-day-old soybean seedling were inoculated with single or mixed strains of Agrobacterium tumefaciens. Mixed-strain infections with the supervirulent L,L-succinamopine type strain A281 (pTiBo542) and strain LBA4404 carrying an octopine type virulence (vir) region and a binary vector (pBin6) with a chimeric gene for kanamycin detoxification gave rise to tumors of which 25% were both kanamycin resistant and capable of hormone-independent growth. Single-strain inoculations with LBA4404 (pBin6) failed to give rise to kanamycin-resistant callus. Syringaldehyde, a compound which induces vir genes carried on the Ti plasmid, increased the number of galls incited on excised cotyledons by the weakly virulent octopine type strain A348 (pTiA6). Similar results were obtained with whole plants treated with this strain in the presence of the vir-inducing compound acetosyringone. Our results indicate that the recovery of transformed soybean cells can be enabled in some instances by coinfecting with a supervirulent strain or in other instances promoted by adding a phenolic compound to the inoculum. |
| doi_str_mv | 10.1104/pp.88.3.570 |
| format | Article |
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Mixed-strain infections with the supervirulent L,L-succinamopine type strain A281 (pTiBo542) and strain LBA4404 carrying an octopine type virulence (vir) region and a binary vector (pBin6) with a chimeric gene for kanamycin detoxification gave rise to tumors of which 25% were both kanamycin resistant and capable of hormone-independent growth. Single-strain inoculations with LBA4404 (pBin6) failed to give rise to kanamycin-resistant callus. Syringaldehyde, a compound which induces vir genes carried on the Ti plasmid, increased the number of galls incited on excised cotyledons by the weakly virulent octopine type strain A348 (pTiA6). Similar results were obtained with whole plants treated with this strain in the presence of the vir-inducing compound acetosyringone. Our results indicate that the recovery of transformed soybean cells can be enabled in some instances by coinfecting with a supervirulent strain or in other instances promoted by adding a phenolic compound to the inoculum.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.88.3.570</identifier><identifier>PMID: 16666350</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>AGROBACTERIUM ; Agrobacterium tumefaciens ; Biological and medical sciences ; Callus ; COMPOSE PHENOLIQUE ; COMPUESTOS FENOLICOS ; Cotyledons ; Fundamental and applied biological sciences. Psychology ; GENETIC TRANSFORMATION ; Genic rearrangement. Recombination. Transposable element ; GLYCINE MAX ; Infections ; Microbe-Plant Interactions ; Molecular and cellular biology ; Molecular genetics ; PHENOLIC COMPOUNDS ; Plant cells ; Plant gall ; Plant tumor inducing plasmids ; PLASMIDE ; PLASMIDIOS ; PLASMIDS ; Soybeans ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; VIRULENCE ; VIRULENCIA</subject><ispartof>Plant physiology (Bethesda), 1988-11, Vol.88 (3), p.570-573</ispartof><rights>Copyright 1988 The American Society of Plant Physiologists</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-49c2709f23c44d53e64a700a6684a62ec6e0ab49fe1da0832841cb882d787f13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4271621$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4271621$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27915,27916,58008,58241</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7364086$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16666350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Owens, L.D</creatorcontrib><creatorcontrib>Smigocki, A.C</creatorcontrib><title>Transformation of soybean cells using mixed strains of Agrobacterium tumefaciens and phenolic compounds</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Cotyledon explants from germinated 1-day-old soybean seedling were inoculated with single or mixed strains of Agrobacterium tumefaciens. Mixed-strain infections with the supervirulent L,L-succinamopine type strain A281 (pTiBo542) and strain LBA4404 carrying an octopine type virulence (vir) region and a binary vector (pBin6) with a chimeric gene for kanamycin detoxification gave rise to tumors of which 25% were both kanamycin resistant and capable of hormone-independent growth. Single-strain inoculations with LBA4404 (pBin6) failed to give rise to kanamycin-resistant callus. Syringaldehyde, a compound which induces vir genes carried on the Ti plasmid, increased the number of galls incited on excised cotyledons by the weakly virulent octopine type strain A348 (pTiA6). Similar results were obtained with whole plants treated with this strain in the presence of the vir-inducing compound acetosyringone. Our results indicate that the recovery of transformed soybean cells can be enabled in some instances by coinfecting with a supervirulent strain or in other instances promoted by adding a phenolic compound to the inoculum.</description><subject>AGROBACTERIUM</subject><subject>Agrobacterium tumefaciens</subject><subject>Biological and medical sciences</subject><subject>Callus</subject><subject>COMPOSE PHENOLIQUE</subject><subject>COMPUESTOS FENOLICOS</subject><subject>Cotyledons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENETIC TRANSFORMATION</subject><subject>Genic rearrangement. Recombination. Transposable element</subject><subject>GLYCINE MAX</subject><subject>Infections</subject><subject>Microbe-Plant Interactions</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>PHENOLIC COMPOUNDS</subject><subject>Plant cells</subject><subject>Plant gall</subject><subject>Plant tumor inducing plasmids</subject><subject>PLASMIDE</subject><subject>PLASMIDIOS</subject><subject>PLASMIDS</subject><subject>Soybeans</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><subject>VIRULENCE</subject><subject>VIRULENCIA</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNp9kc-L1DAcxYMo7rh68iYiPch6kBnzu-llYVlcFRY8OJ7Dt2nSzdImNWnF_e9NmWHUi7kk4X14vO_3IfSS4B0hmH-Ypp1SO7YTNX6ENkQwuqWCq8dog3F5Y6WaM_Qs53uMMWGEP0VnRJbDBN6gfp8gZBfTCLOPoYquyvGhtRAqY4chV0v2oa9G_8t2VZ4T-JBX6KpPsQUz2-SXsZqX0Tow3hYRQldNdzbEwZvKxHGKS-jyc_TEwZDti-N9jvY3H_fXn7e3Xz99ub663RrOxbzljaE1bhxl5d8JZiWHGmOQUnGQ1BppMbS8cZZ0gBWjihPTKkW7WtWOsHN0ebCdlna0nbGhRB70lPwI6UFH8PpfJfg73cefmmAhJJXF4N3RIMUfi82zHn1eNwHBxiXrmjHeME5wIS_-SxJBuRTNmun9ATQp5pysO8UhWK8N6mnSSmmmS4OFfvP3BH_YY2UFeHsEIBsYXOnP-HziaiY5Vusgrw_YfZ5jOsmc1kTSNdSrg-wgauhTcfj-Tamy5pqw3zDMt10</recordid><startdate>19881101</startdate><enddate>19881101</enddate><creator>Owens, L.D</creator><creator>Smigocki, A.C</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19881101</creationdate><title>Transformation of soybean cells using mixed strains of Agrobacterium tumefaciens and phenolic compounds</title><author>Owens, L.D ; Smigocki, A.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-49c2709f23c44d53e64a700a6684a62ec6e0ab49fe1da0832841cb882d787f13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>AGROBACTERIUM</topic><topic>Agrobacterium tumefaciens</topic><topic>Biological and medical sciences</topic><topic>Callus</topic><topic>COMPOSE PHENOLIQUE</topic><topic>COMPUESTOS FENOLICOS</topic><topic>Cotyledons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENETIC TRANSFORMATION</topic><topic>Genic rearrangement. Recombination. Transposable element</topic><topic>GLYCINE MAX</topic><topic>Infections</topic><topic>Microbe-Plant Interactions</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>PHENOLIC COMPOUNDS</topic><topic>Plant cells</topic><topic>Plant gall</topic><topic>Plant tumor inducing plasmids</topic><topic>PLASMIDE</topic><topic>PLASMIDIOS</topic><topic>PLASMIDS</topic><topic>Soybeans</topic><topic>TRANSFORMACION GENETICA</topic><topic>TRANSFORMATION GENETIQUE</topic><topic>VIRULENCE</topic><topic>VIRULENCIA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Owens, L.D</creatorcontrib><creatorcontrib>Smigocki, A.C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Owens, L.D</au><au>Smigocki, A.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transformation of soybean cells using mixed strains of Agrobacterium tumefaciens and phenolic compounds</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1988-11-01</date><risdate>1988</risdate><volume>88</volume><issue>3</issue><spage>570</spage><epage>573</epage><pages>570-573</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Cotyledon explants from germinated 1-day-old soybean seedling were inoculated with single or mixed strains of Agrobacterium tumefaciens. Mixed-strain infections with the supervirulent L,L-succinamopine type strain A281 (pTiBo542) and strain LBA4404 carrying an octopine type virulence (vir) region and a binary vector (pBin6) with a chimeric gene for kanamycin detoxification gave rise to tumors of which 25% were both kanamycin resistant and capable of hormone-independent growth. Single-strain inoculations with LBA4404 (pBin6) failed to give rise to kanamycin-resistant callus. Syringaldehyde, a compound which induces vir genes carried on the Ti plasmid, increased the number of galls incited on excised cotyledons by the weakly virulent octopine type strain A348 (pTiA6). Similar results were obtained with whole plants treated with this strain in the presence of the vir-inducing compound acetosyringone. Our results indicate that the recovery of transformed soybean cells can be enabled in some instances by coinfecting with a supervirulent strain or in other instances promoted by adding a phenolic compound to the inoculum.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16666350</pmid><doi>10.1104/pp.88.3.570</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1988-11, Vol.88 (3), p.570-573 |
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| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Jstor Complete Legacy; Alma/SFX Local Collection |
| subjects | AGROBACTERIUM Agrobacterium tumefaciens Biological and medical sciences Callus COMPOSE PHENOLIQUE COMPUESTOS FENOLICOS Cotyledons Fundamental and applied biological sciences. Psychology GENETIC TRANSFORMATION Genic rearrangement. Recombination. Transposable element GLYCINE MAX Infections Microbe-Plant Interactions Molecular and cellular biology Molecular genetics PHENOLIC COMPOUNDS Plant cells Plant gall Plant tumor inducing plasmids PLASMIDE PLASMIDIOS PLASMIDS Soybeans TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE VIRULENCE VIRULENCIA |
| title | Transformation of soybean cells using mixed strains of Agrobacterium tumefaciens and phenolic compounds |
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