Flavobacterium okayamense sp. nov. isolated from surface seawater

Strain KK2020170 T , a Gram-stain negative, yellow colony-forming bacterium, was isolated from surface seawater sampled in Kojima Bay, Okayama, Japan. Phylogenetic analysis based on the 16S rRNA gene revealed that strain KK2020170 T belongs to the genus Flavobacterium , with Flavobacterium haoranii LQY-7 T (98.1% similarity) being its closest relative, followed by Flavobacterium sediminis MEBiC07310 T (96.9%) and Flavobacterium urocaniciphilum YIT 12746 T (96.0%). Whole-genome shotgun sequencing showed that strain KK2020170 T , when paralleled with F. haoranii LQY-7 T , had 81.3% average nucleotide identity, and 24.6% in silico DNA–DNA hybridization values, respectively. The DNA G + C content of strain KK2020170 T was 31.1 mol%. The most abundant fatty acids (> 10%) of strain KK2020170 T were iso-C15: 0, iso-C17: 0 3-OH and iso-C15: 1 G. The dominant respiratory quinone of the strain was menaquinone MK-6. Based on the phylogenetic and phenotypic analysis results, we propose that strain KK2020170 T represents a novel species, for which the name Flavobacterium okayamense sp. nov. has been proposed. The type strain is KK2020170 T (= ATCC TSD-280 T = NBRC 115344 T ).