Roles of Nrf2/HO-1 and ICAM-1 in the Protective Effect of Nano-Curcumin against Copper-Induced Lung Injury
Copper (Cu) is an essential trace element for maintaining normal homeostasis in living organisms. Yet, an elevated level of Cu beyond homeostatic capacity may lead to oxidative damage of cellular components in several organs, including the lungs. This work investigated the effects of curcumin (Curc)...
Gespeichert in:
| Veröffentlicht in: | International journal of molecular sciences 2023-09, Vol.24 (18), p.13975 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 18 |
| container_start_page | 13975 |
| container_title | International journal of molecular sciences |
| container_volume | 24 |
| creator | Sarawi, Wedad S Alhusaini, Ahlam M Alghibiwi, Hanan K Alsaab, Juman S Hasan, Iman H |
| description | Copper (Cu) is an essential trace element for maintaining normal homeostasis in living organisms. Yet, an elevated level of Cu beyond homeostatic capacity may lead to oxidative damage of cellular components in several organs, including the lungs. This work investigated the effects of curcumin (Curc) and nano-curcumin (nCurc) against Cu-induced lung injury, accenting the roles of oxidative stress, inflammation, and the nuclear factor erythroid 2-related factor/heme oxygenase-1 Nrf2/HO-1 pathway. Rats were challenged with 100 mg/kg of copper sulfate (CuSO4) while being treated with Curc or nCurc for 7 days. Cu-triggered lung oxidative stress detected as dysregulation of oxidative/antioxidant markers, a downregulation of Nrf-2/HO-1 signaling, and an increase in the inflammatory markers interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and intracellular adhesion molecule-1 (ICAM-1). Additionally, it decreased the expression of lung-specific proteins, surfactant protein-C (SP-C), and mucin-1 (MUC-1), induced apoptosis, and caused changes in lung histology. Curc and nCurc alleviated CuSO4-induced lung injury by suppressing oxidative damage and inflammation and activating Nrf-2/HO-1. They also prevented apoptosis and restored the normal expression of SP-C and MUC-1. We concluded that nCurc exhibited superior efficacy compared with Curc in mitigating CuSO4-induced lung injury. This was associated with reduced oxidative stress, inflammation, and apoptotic responses and increased Nrf2/HO-1 signaling and expression of SP-C and MUC-1. |
| doi_str_mv | 10.3390/ijms241813975 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10531221</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A779738051</galeid><sourcerecordid>A779738051</sourcerecordid><originalsourceid>FETCH-LOGICAL-c416t-9f39b419b22128edbfa16fdda4fb90b14fe155b4f189f872cf95d599c209f2783</originalsourceid><addsrcrecordid>eNptks1r3DAQxU1poWmaY-6CXnpxog_bsk4lLEmzsG1CaM9ClkYbGVvaSlYg_321SUi7peigx-g37zFiquqU4DPGBD5345xoQ3rCBG_fVEekobTGuONv_9Lvqw8pjRhTRltxVI13YYKEgkXfo6Xn1zc1QcobtF5dfCvSebTcA7qNYQG9uAdAl9YW9dSgfKhXOeo8F0xtlfNpQauw20Gs195kDQZtst-itR9zfPxYvbNqSnDych9XP68uf6yu683N1xK3qXVDuqUWlomhIWKglNAezGAV6awxqrGDwANpLJC2HRpLemF7TrUVrWmF0BQLS3nPjqsvz767PMxgNPglqknuoptVfJRBOXn44t293IYHSXDLSEktDp9fHGL4lSEtcnZJwzQpDyEnSXuOyycT3hX00z_oGHL0Zb5CdYJ1PWXiD7VVE0jnbSjBem8qLzgXnPW43cee_Ycqx8DsdPBgXakfNNTPDTqGlCLY1yEJlvuVkAcrwX4D-72nTQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2869368239</pqid></control><display><type>article</type><title>Roles of Nrf2/HO-1 and ICAM-1 in the Protective Effect of Nano-Curcumin against Copper-Induced Lung Injury</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Sarawi, Wedad S ; Alhusaini, Ahlam M ; Alghibiwi, Hanan K ; Alsaab, Juman S ; Hasan, Iman H</creator><creatorcontrib>Sarawi, Wedad S ; Alhusaini, Ahlam M ; Alghibiwi, Hanan K ; Alsaab, Juman S ; Hasan, Iman H</creatorcontrib><description>Copper (Cu) is an essential trace element for maintaining normal homeostasis in living organisms. Yet, an elevated level of Cu beyond homeostatic capacity may lead to oxidative damage of cellular components in several organs, including the lungs. This work investigated the effects of curcumin (Curc) and nano-curcumin (nCurc) against Cu-induced lung injury, accenting the roles of oxidative stress, inflammation, and the nuclear factor erythroid 2-related factor/heme oxygenase-1 Nrf2/HO-1 pathway. Rats were challenged with 100 mg/kg of copper sulfate (CuSO4) while being treated with Curc or nCurc for 7 days. Cu-triggered lung oxidative stress detected as dysregulation of oxidative/antioxidant markers, a downregulation of Nrf-2/HO-1 signaling, and an increase in the inflammatory markers interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and intracellular adhesion molecule-1 (ICAM-1). Additionally, it decreased the expression of lung-specific proteins, surfactant protein-C (SP-C), and mucin-1 (MUC-1), induced apoptosis, and caused changes in lung histology. Curc and nCurc alleviated CuSO4-induced lung injury by suppressing oxidative damage and inflammation and activating Nrf-2/HO-1. They also prevented apoptosis and restored the normal expression of SP-C and MUC-1. We concluded that nCurc exhibited superior efficacy compared with Curc in mitigating CuSO4-induced lung injury. This was associated with reduced oxidative stress, inflammation, and apoptotic responses and increased Nrf2/HO-1 signaling and expression of SP-C and MUC-1.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms241813975</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Antioxidants ; Apoptosis ; Chronic obstructive pulmonary disease ; Enzymes ; Gene expression ; Homeostasis ; Inflammation ; Kinases ; Lipid peroxidation ; Lung diseases ; Lungs ; Metabolism ; Nitric oxide ; Oxidative stress ; Physiological aspects ; Proteins ; Toxicity ; Trientine</subject><ispartof>International journal of molecular sciences, 2023-09, Vol.24 (18), p.13975</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c416t-9f39b419b22128edbfa16fdda4fb90b14fe155b4f189f872cf95d599c209f2783</cites><orcidid>0000-0001-6376-9464 ; 0000-0003-2905-8882 ; 0000-0001-7311-1412</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531221/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10531221/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Sarawi, Wedad S</creatorcontrib><creatorcontrib>Alhusaini, Ahlam M</creatorcontrib><creatorcontrib>Alghibiwi, Hanan K</creatorcontrib><creatorcontrib>Alsaab, Juman S</creatorcontrib><creatorcontrib>Hasan, Iman H</creatorcontrib><title>Roles of Nrf2/HO-1 and ICAM-1 in the Protective Effect of Nano-Curcumin against Copper-Induced Lung Injury</title><title>International journal of molecular sciences</title><description>Copper (Cu) is an essential trace element for maintaining normal homeostasis in living organisms. Yet, an elevated level of Cu beyond homeostatic capacity may lead to oxidative damage of cellular components in several organs, including the lungs. This work investigated the effects of curcumin (Curc) and nano-curcumin (nCurc) against Cu-induced lung injury, accenting the roles of oxidative stress, inflammation, and the nuclear factor erythroid 2-related factor/heme oxygenase-1 Nrf2/HO-1 pathway. Rats were challenged with 100 mg/kg of copper sulfate (CuSO4) while being treated with Curc or nCurc for 7 days. Cu-triggered lung oxidative stress detected as dysregulation of oxidative/antioxidant markers, a downregulation of Nrf-2/HO-1 signaling, and an increase in the inflammatory markers interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and intracellular adhesion molecule-1 (ICAM-1). Additionally, it decreased the expression of lung-specific proteins, surfactant protein-C (SP-C), and mucin-1 (MUC-1), induced apoptosis, and caused changes in lung histology. Curc and nCurc alleviated CuSO4-induced lung injury by suppressing oxidative damage and inflammation and activating Nrf-2/HO-1. They also prevented apoptosis and restored the normal expression of SP-C and MUC-1. We concluded that nCurc exhibited superior efficacy compared with Curc in mitigating CuSO4-induced lung injury. This was associated with reduced oxidative stress, inflammation, and apoptotic responses and increased Nrf2/HO-1 signaling and expression of SP-C and MUC-1.</description><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Chronic obstructive pulmonary disease</subject><subject>Enzymes</subject><subject>Gene expression</subject><subject>Homeostasis</subject><subject>Inflammation</subject><subject>Kinases</subject><subject>Lipid peroxidation</subject><subject>Lung diseases</subject><subject>Lungs</subject><subject>Metabolism</subject><subject>Nitric oxide</subject><subject>Oxidative stress</subject><subject>Physiological aspects</subject><subject>Proteins</subject><subject>Toxicity</subject><subject>Trientine</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptks1r3DAQxU1poWmaY-6CXnpxog_bsk4lLEmzsG1CaM9ClkYbGVvaSlYg_321SUi7peigx-g37zFiquqU4DPGBD5345xoQ3rCBG_fVEekobTGuONv_9Lvqw8pjRhTRltxVI13YYKEgkXfo6Xn1zc1QcobtF5dfCvSebTcA7qNYQG9uAdAl9YW9dSgfKhXOeo8F0xtlfNpQauw20Gs195kDQZtst-itR9zfPxYvbNqSnDych9XP68uf6yu683N1xK3qXVDuqUWlomhIWKglNAezGAV6awxqrGDwANpLJC2HRpLemF7TrUVrWmF0BQLS3nPjqsvz767PMxgNPglqknuoptVfJRBOXn44t293IYHSXDLSEktDp9fHGL4lSEtcnZJwzQpDyEnSXuOyycT3hX00z_oGHL0Zb5CdYJ1PWXiD7VVE0jnbSjBem8qLzgXnPW43cee_Ycqx8DsdPBgXakfNNTPDTqGlCLY1yEJlvuVkAcrwX4D-72nTQ</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Sarawi, Wedad S</creator><creator>Alhusaini, Ahlam M</creator><creator>Alghibiwi, Hanan K</creator><creator>Alsaab, Juman S</creator><creator>Hasan, Iman H</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>COVID</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-6376-9464</orcidid><orcidid>https://orcid.org/0000-0003-2905-8882</orcidid><orcidid>https://orcid.org/0000-0001-7311-1412</orcidid></search><sort><creationdate>20230901</creationdate><title>Roles of Nrf2/HO-1 and ICAM-1 in the Protective Effect of Nano-Curcumin against Copper-Induced Lung Injury</title><author>Sarawi, Wedad S ; Alhusaini, Ahlam M ; Alghibiwi, Hanan K ; Alsaab, Juman S ; Hasan, Iman H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-9f39b419b22128edbfa16fdda4fb90b14fe155b4f189f872cf95d599c209f2783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Antioxidants</topic><topic>Apoptosis</topic><topic>Chronic obstructive pulmonary disease</topic><topic>Enzymes</topic><topic>Gene expression</topic><topic>Homeostasis</topic><topic>Inflammation</topic><topic>Kinases</topic><topic>Lipid peroxidation</topic><topic>Lung diseases</topic><topic>Lungs</topic><topic>Metabolism</topic><topic>Nitric oxide</topic><topic>Oxidative stress</topic><topic>Physiological aspects</topic><topic>Proteins</topic><topic>Toxicity</topic><topic>Trientine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sarawi, Wedad S</creatorcontrib><creatorcontrib>Alhusaini, Ahlam M</creatorcontrib><creatorcontrib>Alghibiwi, Hanan K</creatorcontrib><creatorcontrib>Alsaab, Juman S</creatorcontrib><creatorcontrib>Hasan, Iman H</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Coronavirus Research Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sarawi, Wedad S</au><au>Alhusaini, Ahlam M</au><au>Alghibiwi, Hanan K</au><au>Alsaab, Juman S</au><au>Hasan, Iman H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Roles of Nrf2/HO-1 and ICAM-1 in the Protective Effect of Nano-Curcumin against Copper-Induced Lung Injury</atitle><jtitle>International journal of molecular sciences</jtitle><date>2023-09-01</date><risdate>2023</risdate><volume>24</volume><issue>18</issue><spage>13975</spage><pages>13975-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Copper (Cu) is an essential trace element for maintaining normal homeostasis in living organisms. Yet, an elevated level of Cu beyond homeostatic capacity may lead to oxidative damage of cellular components in several organs, including the lungs. This work investigated the effects of curcumin (Curc) and nano-curcumin (nCurc) against Cu-induced lung injury, accenting the roles of oxidative stress, inflammation, and the nuclear factor erythroid 2-related factor/heme oxygenase-1 Nrf2/HO-1 pathway. Rats were challenged with 100 mg/kg of copper sulfate (CuSO4) while being treated with Curc or nCurc for 7 days. Cu-triggered lung oxidative stress detected as dysregulation of oxidative/antioxidant markers, a downregulation of Nrf-2/HO-1 signaling, and an increase in the inflammatory markers interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and intracellular adhesion molecule-1 (ICAM-1). Additionally, it decreased the expression of lung-specific proteins, surfactant protein-C (SP-C), and mucin-1 (MUC-1), induced apoptosis, and caused changes in lung histology. Curc and nCurc alleviated CuSO4-induced lung injury by suppressing oxidative damage and inflammation and activating Nrf-2/HO-1. They also prevented apoptosis and restored the normal expression of SP-C and MUC-1. We concluded that nCurc exhibited superior efficacy compared with Curc in mitigating CuSO4-induced lung injury. This was associated with reduced oxidative stress, inflammation, and apoptotic responses and increased Nrf2/HO-1 signaling and expression of SP-C and MUC-1.</abstract><cop>Basel</cop><pub>MDPI AG</pub><doi>10.3390/ijms241813975</doi><orcidid>https://orcid.org/0000-0001-6376-9464</orcidid><orcidid>https://orcid.org/0000-0003-2905-8882</orcidid><orcidid>https://orcid.org/0000-0001-7311-1412</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1422-0067 |
| ispartof | International journal of molecular sciences, 2023-09, Vol.24 (18), p.13975 |
| issn | 1422-0067 1661-6596 1422-0067 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10531221 |
| source | MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Antioxidants Apoptosis Chronic obstructive pulmonary disease Enzymes Gene expression Homeostasis Inflammation Kinases Lipid peroxidation Lung diseases Lungs Metabolism Nitric oxide Oxidative stress Physiological aspects Proteins Toxicity Trientine |
| title | Roles of Nrf2/HO-1 and ICAM-1 in the Protective Effect of Nano-Curcumin against Copper-Induced Lung Injury |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T16%3A41%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Roles%20of%20Nrf2/HO-1%20and%20ICAM-1%20in%20the%20Protective%20Effect%20of%20Nano-Curcumin%20against%20Copper-Induced%20Lung%20Injury&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Sarawi,%20Wedad%20S&rft.date=2023-09-01&rft.volume=24&rft.issue=18&rft.spage=13975&rft.pages=13975-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms241813975&rft_dat=%3Cgale_pubme%3EA779738051%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2869368239&rft_id=info:pmid/&rft_galeid=A779738051&rfr_iscdi=true |