Restricted feeding modulates peripheral clocks and nutrient sensing pathways in rats
Feeding restriction in rats alters the oscillators in suprachiasmatic, paraventricular, and arcuate nuclei, hypothalamic areas involved in food intake. In the present study, using the same animals and experimental protocol, we aimed to analyze if food restriction could reset clock genes ( ) and gene...
Gespeichert in:
| Veröffentlicht in: | Archives of Endocrinology and Metabolism 2021-10, Vol.65 (5), p.549-561 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 561 |
|---|---|
| container_issue | 5 |
| container_start_page | 549 |
| container_title | Archives of Endocrinology and Metabolism |
| container_volume | 65 |
| creator | Rodrigues, Luis Guilherme F de Araujo, Leonardo D Roa, Silvia L R Bueno, Ana C Uchoa, Ernane T Antunes-Rodrigues, José Moreira, Ayrton C Elias, Lucila L K de Castro, Margaret Martins, Clarissa S |
| description | Feeding restriction in rats alters the oscillators in suprachiasmatic, paraventricular, and arcuate nuclei, hypothalamic areas involved in food intake. In the present study, using the same animals and experimental protocol, we aimed to analyze if food restriction could reset clock genes (
) and genes involved in lipid metabolism (
) through nutrient-sensing pathways (
) in peripheral tissues.
Rats were grouped according to food access: Control group (CG, food
), Restricted night-fed (RF-n, food access during 2 h at night), Restricted day-fed (RF-d, food access during 2 h in the daytime), and Day-fed (DF, food access during 12 h in the daytime). After 21 days, rats were decapitated at ZT3 (0900-1000 h), ZT11 (1700-1800 h), or ZT17 (2300-2400 h). Blood, liver, brown (BAT) and peri-epididymal (PAT) adipose tissues were collected. Plasma corticosterone and gene expression were evaluated by radioimmunoassay and qPCR, respectively.
In the liver, the expression pattern of
and
shifted when food access was dissociated from rat nocturnal activity; this phenomenon was attenuated in adipose tissues. Daytime feeding also inverted the profile of energy-sensing and lipid metabolism-related genes in the liver, whereas calorie restriction induced a pre-feeding increased expression of these genes. In adipose tissues,
expression was modified by daytime feeding and calorie restriction, with concomitant expression of
and
but not
and
.
Feeding restriction reset clock genes and genes involved in lipid metabolism through nutrient-sensing-related genes in rat liver, brown, and peri-epididymal adipose tissues. |
| doi_str_mv | 10.20945/2359-3997000000407 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_doaj_</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10528573</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_c73cb6b4c6684d72b2e62f2cfc4ddddf</doaj_id><sourcerecordid>34591411</sourcerecordid><originalsourceid>FETCH-LOGICAL-c461t-82f15e3b782b5e79e0b83c3daa33799f24592c3354f9fb2fef79775794d446123</originalsourceid><addsrcrecordid>eNpVkdtKAzEQhoMoKrVPIEheoLo5bTZXIsVDQRCkXodsMmm3bneXZKv49qbbWuzczDCZ_5sMP0LXJLulmeLijjKhJkwpmQ3BM3mCLocmp4qe7uvtwAUax7hKM0QQQrg4RxeMC0U4IZdo_g6xD5XtwWEP4Kpmgdet29Smh4g7CFW3hGBqbOvWfkZsGoebTVJA0-MITdwKOtMvv81PxFWDg-njFTrzpo4w3ucR-nh6nE9fJq9vz7Ppw-vE8pz0k4J6IoCVsqClAKkgKwtmmTOGMamUp-mX1DImuFe-pB68VFIKqbjjCUDZCM12XNeale5CtTbhR7em0kOjDQttQl_ZGrSVzJZ5yW2eF9xJWlLIqafWW-5S-MS637G6TbkGZ9N96ewj6PFLUy31ov3SJBO0EJIlAtsRbGhjDOAPYpLpwTS99UQfmZZUN__3HjR_FrFfBwuUGA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Restricted feeding modulates peripheral clocks and nutrient sensing pathways in rats</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Rodrigues, Luis Guilherme F ; de Araujo, Leonardo D ; Roa, Silvia L R ; Bueno, Ana C ; Uchoa, Ernane T ; Antunes-Rodrigues, José ; Moreira, Ayrton C ; Elias, Lucila L K ; de Castro, Margaret ; Martins, Clarissa S</creator><creatorcontrib>Rodrigues, Luis Guilherme F ; de Araujo, Leonardo D ; Roa, Silvia L R ; Bueno, Ana C ; Uchoa, Ernane T ; Antunes-Rodrigues, José ; Moreira, Ayrton C ; Elias, Lucila L K ; de Castro, Margaret ; Martins, Clarissa S</creatorcontrib><description>Feeding restriction in rats alters the oscillators in suprachiasmatic, paraventricular, and arcuate nuclei, hypothalamic areas involved in food intake. In the present study, using the same animals and experimental protocol, we aimed to analyze if food restriction could reset clock genes (
) and genes involved in lipid metabolism (
) through nutrient-sensing pathways (
) in peripheral tissues.
Rats were grouped according to food access: Control group (CG, food
), Restricted night-fed (RF-n, food access during 2 h at night), Restricted day-fed (RF-d, food access during 2 h in the daytime), and Day-fed (DF, food access during 12 h in the daytime). After 21 days, rats were decapitated at ZT3 (0900-1000 h), ZT11 (1700-1800 h), or ZT17 (2300-2400 h). Blood, liver, brown (BAT) and peri-epididymal (PAT) adipose tissues were collected. Plasma corticosterone and gene expression were evaluated by radioimmunoassay and qPCR, respectively.
In the liver, the expression pattern of
and
shifted when food access was dissociated from rat nocturnal activity; this phenomenon was attenuated in adipose tissues. Daytime feeding also inverted the profile of energy-sensing and lipid metabolism-related genes in the liver, whereas calorie restriction induced a pre-feeding increased expression of these genes. In adipose tissues,
expression was modified by daytime feeding and calorie restriction, with concomitant expression of
and
but not
and
.
Feeding restriction reset clock genes and genes involved in lipid metabolism through nutrient-sensing-related genes in rat liver, brown, and peri-epididymal adipose tissues.</description><identifier>ISSN: 2359-3997</identifier><identifier>EISSN: 2359-4292</identifier><identifier>DOI: 10.20945/2359-3997000000407</identifier><identifier>PMID: 34591411</identifier><language>eng</language><publisher>Brazil: Sociedade Brasileira de Endocrinologia e Metabologia</publisher><subject>Animals ; biological clocks ; Caloric restriction ; Circadian Rhythm ; feeding behavior ; Hypothalamus ; Lipid Metabolism ; Liver - metabolism ; Nutrients ; Original ; Rats ; sirtuin 1</subject><ispartof>Archives of Endocrinology and Metabolism, 2021-10, Vol.65 (5), p.549-561</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c461t-82f15e3b782b5e79e0b83c3daa33799f24592c3354f9fb2fef79775794d446123</cites><orcidid>0000-0003-3486-9217 ; 0000-0002-6271-758X ; 0000-0003-1295-5333 ; 0000-0002-8202-5118 ; 0000-0002-3555-4404 ; 0000-0001-9043-9831 ; 0000-0002-8096-6803 ; 0000-0002-3601-9470 ; 0000-0003-4932-4623 ; 0000-0002-4649-0944</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10528573/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10528573/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34591411$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodrigues, Luis Guilherme F</creatorcontrib><creatorcontrib>de Araujo, Leonardo D</creatorcontrib><creatorcontrib>Roa, Silvia L R</creatorcontrib><creatorcontrib>Bueno, Ana C</creatorcontrib><creatorcontrib>Uchoa, Ernane T</creatorcontrib><creatorcontrib>Antunes-Rodrigues, José</creatorcontrib><creatorcontrib>Moreira, Ayrton C</creatorcontrib><creatorcontrib>Elias, Lucila L K</creatorcontrib><creatorcontrib>de Castro, Margaret</creatorcontrib><creatorcontrib>Martins, Clarissa S</creatorcontrib><title>Restricted feeding modulates peripheral clocks and nutrient sensing pathways in rats</title><title>Archives of Endocrinology and Metabolism</title><addtitle>Arch Endocrinol Metab</addtitle><description>Feeding restriction in rats alters the oscillators in suprachiasmatic, paraventricular, and arcuate nuclei, hypothalamic areas involved in food intake. In the present study, using the same animals and experimental protocol, we aimed to analyze if food restriction could reset clock genes (
) and genes involved in lipid metabolism (
) through nutrient-sensing pathways (
) in peripheral tissues.
Rats were grouped according to food access: Control group (CG, food
), Restricted night-fed (RF-n, food access during 2 h at night), Restricted day-fed (RF-d, food access during 2 h in the daytime), and Day-fed (DF, food access during 12 h in the daytime). After 21 days, rats were decapitated at ZT3 (0900-1000 h), ZT11 (1700-1800 h), or ZT17 (2300-2400 h). Blood, liver, brown (BAT) and peri-epididymal (PAT) adipose tissues were collected. Plasma corticosterone and gene expression were evaluated by radioimmunoassay and qPCR, respectively.
In the liver, the expression pattern of
and
shifted when food access was dissociated from rat nocturnal activity; this phenomenon was attenuated in adipose tissues. Daytime feeding also inverted the profile of energy-sensing and lipid metabolism-related genes in the liver, whereas calorie restriction induced a pre-feeding increased expression of these genes. In adipose tissues,
expression was modified by daytime feeding and calorie restriction, with concomitant expression of
and
but not
and
.
Feeding restriction reset clock genes and genes involved in lipid metabolism through nutrient-sensing-related genes in rat liver, brown, and peri-epididymal adipose tissues.</description><subject>Animals</subject><subject>biological clocks</subject><subject>Caloric restriction</subject><subject>Circadian Rhythm</subject><subject>feeding behavior</subject><subject>Hypothalamus</subject><subject>Lipid Metabolism</subject><subject>Liver - metabolism</subject><subject>Nutrients</subject><subject>Original</subject><subject>Rats</subject><subject>sirtuin 1</subject><issn>2359-3997</issn><issn>2359-4292</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>DOA</sourceid><recordid>eNpVkdtKAzEQhoMoKrVPIEheoLo5bTZXIsVDQRCkXodsMmm3bneXZKv49qbbWuzczDCZ_5sMP0LXJLulmeLijjKhJkwpmQ3BM3mCLocmp4qe7uvtwAUax7hKM0QQQrg4RxeMC0U4IZdo_g6xD5XtwWEP4Kpmgdet29Smh4g7CFW3hGBqbOvWfkZsGoebTVJA0-MITdwKOtMvv81PxFWDg-njFTrzpo4w3ucR-nh6nE9fJq9vz7Ppw-vE8pz0k4J6IoCVsqClAKkgKwtmmTOGMamUp-mX1DImuFe-pB68VFIKqbjjCUDZCM12XNeale5CtTbhR7em0kOjDQttQl_ZGrSVzJZ5yW2eF9xJWlLIqafWW-5S-MS637G6TbkGZ9N96ewj6PFLUy31ov3SJBO0EJIlAtsRbGhjDOAPYpLpwTS99UQfmZZUN__3HjR_FrFfBwuUGA</recordid><startdate>20211029</startdate><enddate>20211029</enddate><creator>Rodrigues, Luis Guilherme F</creator><creator>de Araujo, Leonardo D</creator><creator>Roa, Silvia L R</creator><creator>Bueno, Ana C</creator><creator>Uchoa, Ernane T</creator><creator>Antunes-Rodrigues, José</creator><creator>Moreira, Ayrton C</creator><creator>Elias, Lucila L K</creator><creator>de Castro, Margaret</creator><creator>Martins, Clarissa S</creator><general>Sociedade Brasileira de Endocrinologia e Metabologia</general><general>Brazilian Society of Endocrinology and Metabolism</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3486-9217</orcidid><orcidid>https://orcid.org/0000-0002-6271-758X</orcidid><orcidid>https://orcid.org/0000-0003-1295-5333</orcidid><orcidid>https://orcid.org/0000-0002-8202-5118</orcidid><orcidid>https://orcid.org/0000-0002-3555-4404</orcidid><orcidid>https://orcid.org/0000-0001-9043-9831</orcidid><orcidid>https://orcid.org/0000-0002-8096-6803</orcidid><orcidid>https://orcid.org/0000-0002-3601-9470</orcidid><orcidid>https://orcid.org/0000-0003-4932-4623</orcidid><orcidid>https://orcid.org/0000-0002-4649-0944</orcidid></search><sort><creationdate>20211029</creationdate><title>Restricted feeding modulates peripheral clocks and nutrient sensing pathways in rats</title><author>Rodrigues, Luis Guilherme F ; de Araujo, Leonardo D ; Roa, Silvia L R ; Bueno, Ana C ; Uchoa, Ernane T ; Antunes-Rodrigues, José ; Moreira, Ayrton C ; Elias, Lucila L K ; de Castro, Margaret ; Martins, Clarissa S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c461t-82f15e3b782b5e79e0b83c3daa33799f24592c3354f9fb2fef79775794d446123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>biological clocks</topic><topic>Caloric restriction</topic><topic>Circadian Rhythm</topic><topic>feeding behavior</topic><topic>Hypothalamus</topic><topic>Lipid Metabolism</topic><topic>Liver - metabolism</topic><topic>Nutrients</topic><topic>Original</topic><topic>Rats</topic><topic>sirtuin 1</topic><toplevel>online_resources</toplevel><creatorcontrib>Rodrigues, Luis Guilherme F</creatorcontrib><creatorcontrib>de Araujo, Leonardo D</creatorcontrib><creatorcontrib>Roa, Silvia L R</creatorcontrib><creatorcontrib>Bueno, Ana C</creatorcontrib><creatorcontrib>Uchoa, Ernane T</creatorcontrib><creatorcontrib>Antunes-Rodrigues, José</creatorcontrib><creatorcontrib>Moreira, Ayrton C</creatorcontrib><creatorcontrib>Elias, Lucila L K</creatorcontrib><creatorcontrib>de Castro, Margaret</creatorcontrib><creatorcontrib>Martins, Clarissa S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Archives of Endocrinology and Metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodrigues, Luis Guilherme F</au><au>de Araujo, Leonardo D</au><au>Roa, Silvia L R</au><au>Bueno, Ana C</au><au>Uchoa, Ernane T</au><au>Antunes-Rodrigues, José</au><au>Moreira, Ayrton C</au><au>Elias, Lucila L K</au><au>de Castro, Margaret</au><au>Martins, Clarissa S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Restricted feeding modulates peripheral clocks and nutrient sensing pathways in rats</atitle><jtitle>Archives of Endocrinology and Metabolism</jtitle><addtitle>Arch Endocrinol Metab</addtitle><date>2021-10-29</date><risdate>2021</risdate><volume>65</volume><issue>5</issue><spage>549</spage><epage>561</epage><pages>549-561</pages><issn>2359-3997</issn><eissn>2359-4292</eissn><abstract>Feeding restriction in rats alters the oscillators in suprachiasmatic, paraventricular, and arcuate nuclei, hypothalamic areas involved in food intake. In the present study, using the same animals and experimental protocol, we aimed to analyze if food restriction could reset clock genes (
) and genes involved in lipid metabolism (
) through nutrient-sensing pathways (
) in peripheral tissues.
Rats were grouped according to food access: Control group (CG, food
), Restricted night-fed (RF-n, food access during 2 h at night), Restricted day-fed (RF-d, food access during 2 h in the daytime), and Day-fed (DF, food access during 12 h in the daytime). After 21 days, rats were decapitated at ZT3 (0900-1000 h), ZT11 (1700-1800 h), or ZT17 (2300-2400 h). Blood, liver, brown (BAT) and peri-epididymal (PAT) adipose tissues were collected. Plasma corticosterone and gene expression were evaluated by radioimmunoassay and qPCR, respectively.
In the liver, the expression pattern of
and
shifted when food access was dissociated from rat nocturnal activity; this phenomenon was attenuated in adipose tissues. Daytime feeding also inverted the profile of energy-sensing and lipid metabolism-related genes in the liver, whereas calorie restriction induced a pre-feeding increased expression of these genes. In adipose tissues,
expression was modified by daytime feeding and calorie restriction, with concomitant expression of
and
but not
and
.
Feeding restriction reset clock genes and genes involved in lipid metabolism through nutrient-sensing-related genes in rat liver, brown, and peri-epididymal adipose tissues.</abstract><cop>Brazil</cop><pub>Sociedade Brasileira de Endocrinologia e Metabologia</pub><pmid>34591411</pmid><doi>10.20945/2359-3997000000407</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0003-3486-9217</orcidid><orcidid>https://orcid.org/0000-0002-6271-758X</orcidid><orcidid>https://orcid.org/0000-0003-1295-5333</orcidid><orcidid>https://orcid.org/0000-0002-8202-5118</orcidid><orcidid>https://orcid.org/0000-0002-3555-4404</orcidid><orcidid>https://orcid.org/0000-0001-9043-9831</orcidid><orcidid>https://orcid.org/0000-0002-8096-6803</orcidid><orcidid>https://orcid.org/0000-0002-3601-9470</orcidid><orcidid>https://orcid.org/0000-0003-4932-4623</orcidid><orcidid>https://orcid.org/0000-0002-4649-0944</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2359-3997 |
| ispartof | Archives of Endocrinology and Metabolism, 2021-10, Vol.65 (5), p.549-561 |
| issn | 2359-3997 2359-4292 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10528573 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central; PubMed Central Open Access |
| subjects | Animals biological clocks Caloric restriction Circadian Rhythm feeding behavior Hypothalamus Lipid Metabolism Liver - metabolism Nutrients Original Rats sirtuin 1 |
| title | Restricted feeding modulates peripheral clocks and nutrient sensing pathways in rats |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T21%3A37%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Restricted%20feeding%20modulates%20peripheral%20clocks%20and%20nutrient%20sensing%20pathways%20in%20rats&rft.jtitle=Archives%20of%20Endocrinology%20and%20Metabolism&rft.au=Rodrigues,%20Luis%20Guilherme%20F&rft.date=2021-10-29&rft.volume=65&rft.issue=5&rft.spage=549&rft.epage=561&rft.pages=549-561&rft.issn=2359-3997&rft.eissn=2359-4292&rft_id=info:doi/10.20945/2359-3997000000407&rft_dat=%3Cpubmed_doaj_%3E34591411%3C/pubmed_doaj_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/34591411&rft_doaj_id=oai_doaj_org_article_c73cb6b4c6684d72b2e62f2cfc4ddddf&rfr_iscdi=true |