Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin
Objectives To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing / regeneration in recession-type defects. Materials and methods A total of 17 gingival recession-type defects were surgically created in the maxilla of three minipigs. The...
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| Veröffentlicht in: | Clinical oral investigations 2023-09, Vol.27 (9), p.5041-5048 |
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| creator | Chackartchi, Tali Bosshardt, Dieter D. Imber, Jean-Claude Stähli, Alexandra Sacks, Hagit Nagy, Katalin Sculean, Anton |
| description | Objectives
To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing / regeneration in recession-type defects.
Materials and methods
A total of 17 gingival recession-type defects were surgically created in the maxilla of three minipigs. The defects were randomly treated with a coronally advanced flap (CAF) and either rAmelX (test), or a CAF and placebo (control). At three months following reconstructive surgery, the animals were euthanized, and the healing outcomes histologically evaluated.
Results
The test group yielded statistically significantly (
p
= 0.047) greater formation of cementum with inserting collagen fibers compared with the control group (i.e., 4.38 mm ± 0.36 mm vs. 3.48 mm ± 1.13 mm). Bone formation measured 2.15 mm ± 0.8 mm in the test group and 2.24 mm ± 1.23 mm in the control group, respectively, without a statistically significant difference (
p
= 0.94).
Conclusions
The present data have provided for the first-time evidence for the potential of rAmelX to promote regeneration of periodontal ligament and root cementum in recession-type defects, thus warranting further preclinical and clinical testing.
Clinical relevance
The present results set the basis for the potential clinical application of rAmelX in reconstructive periodontal surgery. |
| doi_str_mv | 10.1007/s00784-023-05123-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10492744</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2862848243</sourcerecordid><originalsourceid>FETCH-LOGICAL-c426t-2aacd18d4adf412753d9ef116b5444906f8ee2240afc8dfc9044c8a02f71bb9f3</originalsourceid><addsrcrecordid>eNp9kctuFDEQRVsIRELgB1ggS2zYNPg1_VghFAFBisQG1la1XZ5x5LYH2z3JfEN-Gg8TQmDBpmypTt163KZ5yehbRmn_LtcwyJZy0dIVq_HmUXPKpOha0ffs8YP_SfMs5ytKmex68bQ5Eb3kTI78tLm9cLlEH9dOgye4A79AcTEQG72P1y6sSUkIZcZQSLQkocacK9CW_RaJQYu6ZHLtyobomGIA7_cEzA6CRkOshy2BYAiQEHfoyWaZIRxU4jy5AFUUZqztMbjwvHliwWd8cfeeNd8_ffx2ftFefv385fzDZasl70rLAbRhg5FgrGS8XwkzomWsm1ZSypF2dkDkXFKwejBWj1RKPQDltmfTNFpx1rw_6m6XaUaj62oJvNomN0PaqwhO_Z0JbqPWcacYrTfrpawKb-4UUvyxYC5qdlmj9xAwLlnxQax4T5kYKvr6H_QqLqme6UB1fJADl6JS_EjpFHNOaO-nYVQdzFZHs1U1W_0yW93UolcP97gv-e1uBcQRyDUV1pj-9P6P7E8Jf7pF</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2862848243</pqid></control><display><type>article</type><title>Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin</title><source>SpringerNature Journals</source><creator>Chackartchi, Tali ; Bosshardt, Dieter D. ; Imber, Jean-Claude ; Stähli, Alexandra ; Sacks, Hagit ; Nagy, Katalin ; Sculean, Anton</creator><creatorcontrib>Chackartchi, Tali ; Bosshardt, Dieter D. ; Imber, Jean-Claude ; Stähli, Alexandra ; Sacks, Hagit ; Nagy, Katalin ; Sculean, Anton</creatorcontrib><description>Objectives
To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing / regeneration in recession-type defects.
Materials and methods
A total of 17 gingival recession-type defects were surgically created in the maxilla of three minipigs. The defects were randomly treated with a coronally advanced flap (CAF) and either rAmelX (test), or a CAF and placebo (control). At three months following reconstructive surgery, the animals were euthanized, and the healing outcomes histologically evaluated.
Results
The test group yielded statistically significantly (
p
= 0.047) greater formation of cementum with inserting collagen fibers compared with the control group (i.e., 4.38 mm ± 0.36 mm vs. 3.48 mm ± 1.13 mm). Bone formation measured 2.15 mm ± 0.8 mm in the test group and 2.24 mm ± 1.23 mm in the control group, respectively, without a statistically significant difference (
p
= 0.94).
Conclusions
The present data have provided for the first-time evidence for the potential of rAmelX to promote regeneration of periodontal ligament and root cementum in recession-type defects, thus warranting further preclinical and clinical testing.
Clinical relevance
The present results set the basis for the potential clinical application of rAmelX in reconstructive periodontal surgery.</description><identifier>ISSN: 1436-3771</identifier><identifier>ISSN: 1432-6981</identifier><identifier>EISSN: 1436-3771</identifier><identifier>DOI: 10.1007/s00784-023-05123-x</identifier><identifier>PMID: 37421492</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Bone growth ; Cementum ; Dental enamel ; Dentistry ; Ligaments ; Local anesthesia ; Maxilla ; Medicine ; Osteogenesis ; Periodontal ligament ; Periodontics ; Reconstructive surgery ; Regeneration ; Statistical analysis ; Surgeons ; Surgery ; Sutures ; Wound healing</subject><ispartof>Clinical oral investigations, 2023-09, Vol.27 (9), p.5041-5048</ispartof><rights>The Author(s) 2023</rights><rights>2023. The Author(s).</rights><rights>The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c426t-2aacd18d4adf412753d9ef116b5444906f8ee2240afc8dfc9044c8a02f71bb9f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00784-023-05123-x$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00784-023-05123-x$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,315,782,786,887,27931,27932,41495,42564,51326</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37421492$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chackartchi, Tali</creatorcontrib><creatorcontrib>Bosshardt, Dieter D.</creatorcontrib><creatorcontrib>Imber, Jean-Claude</creatorcontrib><creatorcontrib>Stähli, Alexandra</creatorcontrib><creatorcontrib>Sacks, Hagit</creatorcontrib><creatorcontrib>Nagy, Katalin</creatorcontrib><creatorcontrib>Sculean, Anton</creatorcontrib><title>Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin</title><title>Clinical oral investigations</title><addtitle>Clin Oral Invest</addtitle><addtitle>Clin Oral Investig</addtitle><description>Objectives
To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing / regeneration in recession-type defects.
Materials and methods
A total of 17 gingival recession-type defects were surgically created in the maxilla of three minipigs. The defects were randomly treated with a coronally advanced flap (CAF) and either rAmelX (test), or a CAF and placebo (control). At three months following reconstructive surgery, the animals were euthanized, and the healing outcomes histologically evaluated.
Results
The test group yielded statistically significantly (
p
= 0.047) greater formation of cementum with inserting collagen fibers compared with the control group (i.e., 4.38 mm ± 0.36 mm vs. 3.48 mm ± 1.13 mm). Bone formation measured 2.15 mm ± 0.8 mm in the test group and 2.24 mm ± 1.23 mm in the control group, respectively, without a statistically significant difference (
p
= 0.94).
Conclusions
The present data have provided for the first-time evidence for the potential of rAmelX to promote regeneration of periodontal ligament and root cementum in recession-type defects, thus warranting further preclinical and clinical testing.
Clinical relevance
The present results set the basis for the potential clinical application of rAmelX in reconstructive periodontal surgery.</description><subject>Animals</subject><subject>Bone growth</subject><subject>Cementum</subject><subject>Dental enamel</subject><subject>Dentistry</subject><subject>Ligaments</subject><subject>Local anesthesia</subject><subject>Maxilla</subject><subject>Medicine</subject><subject>Osteogenesis</subject><subject>Periodontal ligament</subject><subject>Periodontics</subject><subject>Reconstructive surgery</subject><subject>Regeneration</subject><subject>Statistical analysis</subject><subject>Surgeons</subject><subject>Surgery</subject><subject>Sutures</subject><subject>Wound healing</subject><issn>1436-3771</issn><issn>1432-6981</issn><issn>1436-3771</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kctuFDEQRVsIRELgB1ggS2zYNPg1_VghFAFBisQG1la1XZ5x5LYH2z3JfEN-Gg8TQmDBpmypTt163KZ5yehbRmn_LtcwyJZy0dIVq_HmUXPKpOha0ffs8YP_SfMs5ytKmex68bQ5Eb3kTI78tLm9cLlEH9dOgye4A79AcTEQG72P1y6sSUkIZcZQSLQkocacK9CW_RaJQYu6ZHLtyobomGIA7_cEzA6CRkOshy2BYAiQEHfoyWaZIRxU4jy5AFUUZqztMbjwvHliwWd8cfeeNd8_ffx2ftFefv385fzDZasl70rLAbRhg5FgrGS8XwkzomWsm1ZSypF2dkDkXFKwejBWj1RKPQDltmfTNFpx1rw_6m6XaUaj62oJvNomN0PaqwhO_Z0JbqPWcacYrTfrpawKb-4UUvyxYC5qdlmj9xAwLlnxQax4T5kYKvr6H_QqLqme6UB1fJADl6JS_EjpFHNOaO-nYVQdzFZHs1U1W_0yW93UolcP97gv-e1uBcQRyDUV1pj-9P6P7E8Jf7pF</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Chackartchi, Tali</creator><creator>Bosshardt, Dieter D.</creator><creator>Imber, Jean-Claude</creator><creator>Stähli, Alexandra</creator><creator>Sacks, Hagit</creator><creator>Nagy, Katalin</creator><creator>Sculean, Anton</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20230901</creationdate><title>Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin</title><author>Chackartchi, Tali ; Bosshardt, Dieter D. ; Imber, Jean-Claude ; Stähli, Alexandra ; Sacks, Hagit ; Nagy, Katalin ; Sculean, Anton</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-2aacd18d4adf412753d9ef116b5444906f8ee2240afc8dfc9044c8a02f71bb9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Bone growth</topic><topic>Cementum</topic><topic>Dental enamel</topic><topic>Dentistry</topic><topic>Ligaments</topic><topic>Local anesthesia</topic><topic>Maxilla</topic><topic>Medicine</topic><topic>Osteogenesis</topic><topic>Periodontal ligament</topic><topic>Periodontics</topic><topic>Reconstructive surgery</topic><topic>Regeneration</topic><topic>Statistical analysis</topic><topic>Surgeons</topic><topic>Surgery</topic><topic>Sutures</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chackartchi, Tali</creatorcontrib><creatorcontrib>Bosshardt, Dieter D.</creatorcontrib><creatorcontrib>Imber, Jean-Claude</creatorcontrib><creatorcontrib>Stähli, Alexandra</creatorcontrib><creatorcontrib>Sacks, Hagit</creatorcontrib><creatorcontrib>Nagy, Katalin</creatorcontrib><creatorcontrib>Sculean, Anton</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical oral investigations</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chackartchi, Tali</au><au>Bosshardt, Dieter D.</au><au>Imber, Jean-Claude</au><au>Stähli, Alexandra</au><au>Sacks, Hagit</au><au>Nagy, Katalin</au><au>Sculean, Anton</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin</atitle><jtitle>Clinical oral investigations</jtitle><stitle>Clin Oral Invest</stitle><addtitle>Clin Oral Investig</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>27</volume><issue>9</issue><spage>5041</spage><epage>5048</epage><pages>5041-5048</pages><issn>1436-3771</issn><issn>1432-6981</issn><eissn>1436-3771</eissn><abstract>Objectives
To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing / regeneration in recession-type defects.
Materials and methods
A total of 17 gingival recession-type defects were surgically created in the maxilla of three minipigs. The defects were randomly treated with a coronally advanced flap (CAF) and either rAmelX (test), or a CAF and placebo (control). At three months following reconstructive surgery, the animals were euthanized, and the healing outcomes histologically evaluated.
Results
The test group yielded statistically significantly (
p
= 0.047) greater formation of cementum with inserting collagen fibers compared with the control group (i.e., 4.38 mm ± 0.36 mm vs. 3.48 mm ± 1.13 mm). Bone formation measured 2.15 mm ± 0.8 mm in the test group and 2.24 mm ± 1.23 mm in the control group, respectively, without a statistically significant difference (
p
= 0.94).
Conclusions
The present data have provided for the first-time evidence for the potential of rAmelX to promote regeneration of periodontal ligament and root cementum in recession-type defects, thus warranting further preclinical and clinical testing.
Clinical relevance
The present results set the basis for the potential clinical application of rAmelX in reconstructive periodontal surgery.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>37421492</pmid><doi>10.1007/s00784-023-05123-x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Clinical oral investigations, 2023-09, Vol.27 (9), p.5041-5048 |
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| language | eng |
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| source | SpringerNature Journals |
| subjects | Animals Bone growth Cementum Dental enamel Dentistry Ligaments Local anesthesia Maxilla Medicine Osteogenesis Periodontal ligament Periodontics Reconstructive surgery Regeneration Statistical analysis Surgeons Surgery Sutures Wound healing |
| title | Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin |
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