Evaluation of drug carrier hepatotoxicity using primary cell culture models
This study aims to establish a primary rat hepatocyte culture model to evaluate dose-dependent hepatotoxic effects of drug carriers (lipopolymer nanoparticles; LPNs) temporal. Primary rat hepatocyte cell cultures were used to determine half-maximal Inhibition Concentrations (IC50) of the drug-carrie...
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| Veröffentlicht in: | Nanomedicine 2023-02, Vol.48, p.102651-102651, Article 102651 |
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| container_title | Nanomedicine |
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| creator | Kibar, Güneş Dutta, Subhadeep Rege, Kaushal Usta, O. Berk |
| description | This study aims to establish a primary rat hepatocyte culture model to evaluate dose-dependent hepatotoxic effects of drug carriers (lipopolymer nanoparticles; LPNs) temporal. Primary rat hepatocyte cell cultures were used to determine half-maximal Inhibition Concentrations (IC50) of the drug-carrier library. Drug-carrier library, at concentrations |
| doi_str_mv | 10.1016/j.nano.2023.102651 |
| format | Article |
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The different rat hepatocyte culture models - a 96-well monolayer culture model and a 12-well monolayer culture model- were investigated to evaluate the hepatotoxicity of nanocarriers. A small library of aminoglycoside-derived polymers and lipopolymers were used as model nanocarriers in different concentrations. Comparing the control group, cell viability and hepatic biomarkers - albumin secretion and urea excretion – were used to determine IC50 values of the different nanocarriers. [Display omitted]</description><identifier>ISSN: 1549-9634</identifier><identifier>EISSN: 1549-9642</identifier><identifier>DOI: 10.1016/j.nano.2023.102651</identifier><identifier>PMID: 36623713</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Albumins ; Animals ; Cells, Cultured ; Chemical and Drug Induced Liver Injury - metabolism ; Drug Carriers - pharmacology ; Hepatocytes ; Hepatotoxicity ; In vitro culture ; Lipopolymer nanoparticle (LPN) ; Nanotoxicity ; Primary Cell Culture ; Primary rat hepatocyte ; Rats ; Urea - metabolism ; Urea - pharmacology</subject><ispartof>Nanomedicine, 2023-02, Vol.48, p.102651-102651, Article 102651</ispartof><rights>2023</rights><rights>Copyright © 2023. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c407t-3b5c02474291e978b88efe102dbb69d992969fec0fbc59cd7b003ea8c82cf5203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.nano.2023.102651$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,315,781,785,886,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36623713$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kibar, Güneş</creatorcontrib><creatorcontrib>Dutta, Subhadeep</creatorcontrib><creatorcontrib>Rege, Kaushal</creatorcontrib><creatorcontrib>Usta, O. Berk</creatorcontrib><title>Evaluation of drug carrier hepatotoxicity using primary cell culture models</title><title>Nanomedicine</title><addtitle>Nanomedicine</addtitle><description>This study aims to establish a primary rat hepatocyte culture model to evaluate dose-dependent hepatotoxic effects of drug carriers (lipopolymer nanoparticles; LPNs) temporal. Primary rat hepatocyte cell cultures were used to determine half-maximal Inhibition Concentrations (IC50) of the drug-carrier library. Drug-carrier library, at concentrations <50 μg/mL, is benign to primary rat hepatocytes as determined using albumin and urea secretions. Albumin, as a hepatic biomarker, exhibited a more sensitive and faster outcome, compared to urea, for the determination of the IC50 value of LPNs. Temporal measurements of hepatic biomarkers including urea and albumin, and rigorous physicochemical (hydrodynamic diameter, surface charge, etc.) characterization, should be combined to evaluate the hepatotoxicity of drug carrier libraries in screens.
The different rat hepatocyte culture models - a 96-well monolayer culture model and a 12-well monolayer culture model- were investigated to evaluate the hepatotoxicity of nanocarriers. A small library of aminoglycoside-derived polymers and lipopolymers were used as model nanocarriers in different concentrations. Comparing the control group, cell viability and hepatic biomarkers - albumin secretion and urea excretion – were used to determine IC50 values of the different nanocarriers. [Display omitted]</description><subject>Albumins</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Chemical and Drug Induced Liver Injury - metabolism</subject><subject>Drug Carriers - pharmacology</subject><subject>Hepatocytes</subject><subject>Hepatotoxicity</subject><subject>In vitro culture</subject><subject>Lipopolymer nanoparticle (LPN)</subject><subject>Nanotoxicity</subject><subject>Primary Cell Culture</subject><subject>Primary rat hepatocyte</subject><subject>Rats</subject><subject>Urea - metabolism</subject><subject>Urea - pharmacology</subject><issn>1549-9634</issn><issn>1549-9642</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kNtKAzEQhoMotlZfwAvJC2zNYU8BQUTqAQve6HXIJrNtynZTstli396UrUVvvJphZv5_Zj6ErimZUkLz29W0Va2bMsJ4LLA8oydoTLNUJCJP2ekx5-kIXXTdihBeECLO0YjnOeMF5WP0NtuqplfBuha7GhvfL7BW3lvweAkbFVxwX1bbsMN9Z9sF3ni7Vn6HNTQN1n0Teg947Qw03SU6q1XTwdUhTtDn0-zj8SWZvz-_Pj7ME52SIiS8yjRhaZEyQUEUZVWWUEN8wFRVLowQTOSiBk3qSmdCm6KKh4Mqdcl0nTHCJ-h-8N301RqMhjZ41cjDZdIpK_92WruUC7eVlKSC5UxEBzY4aO-6zkN9FFMi92zlSu7Zyj1bObCNopvfa4-SH5hx4G4YiCxgGwnKTltoNRjrQQdpnP3P_xvXqI1s</recordid><startdate>20230201</startdate><enddate>20230201</enddate><creator>Kibar, Güneş</creator><creator>Dutta, Subhadeep</creator><creator>Rege, Kaushal</creator><creator>Usta, O. 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Berk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-3b5c02474291e978b88efe102dbb69d992969fec0fbc59cd7b003ea8c82cf5203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Albumins</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Chemical and Drug Induced Liver Injury - metabolism</topic><topic>Drug Carriers - pharmacology</topic><topic>Hepatocytes</topic><topic>Hepatotoxicity</topic><topic>In vitro culture</topic><topic>Lipopolymer nanoparticle (LPN)</topic><topic>Nanotoxicity</topic><topic>Primary Cell Culture</topic><topic>Primary rat hepatocyte</topic><topic>Rats</topic><topic>Urea - metabolism</topic><topic>Urea - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kibar, Güneş</creatorcontrib><creatorcontrib>Dutta, Subhadeep</creatorcontrib><creatorcontrib>Rege, Kaushal</creatorcontrib><creatorcontrib>Usta, O. 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Berk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of drug carrier hepatotoxicity using primary cell culture models</atitle><jtitle>Nanomedicine</jtitle><addtitle>Nanomedicine</addtitle><date>2023-02-01</date><risdate>2023</risdate><volume>48</volume><spage>102651</spage><epage>102651</epage><pages>102651-102651</pages><artnum>102651</artnum><issn>1549-9634</issn><eissn>1549-9642</eissn><abstract>This study aims to establish a primary rat hepatocyte culture model to evaluate dose-dependent hepatotoxic effects of drug carriers (lipopolymer nanoparticles; LPNs) temporal. Primary rat hepatocyte cell cultures were used to determine half-maximal Inhibition Concentrations (IC50) of the drug-carrier library. Drug-carrier library, at concentrations <50 μg/mL, is benign to primary rat hepatocytes as determined using albumin and urea secretions. Albumin, as a hepatic biomarker, exhibited a more sensitive and faster outcome, compared to urea, for the determination of the IC50 value of LPNs. Temporal measurements of hepatic biomarkers including urea and albumin, and rigorous physicochemical (hydrodynamic diameter, surface charge, etc.) characterization, should be combined to evaluate the hepatotoxicity of drug carrier libraries in screens.
The different rat hepatocyte culture models - a 96-well monolayer culture model and a 12-well monolayer culture model- were investigated to evaluate the hepatotoxicity of nanocarriers. A small library of aminoglycoside-derived polymers and lipopolymers were used as model nanocarriers in different concentrations. Comparing the control group, cell viability and hepatic biomarkers - albumin secretion and urea excretion – were used to determine IC50 values of the different nanocarriers. [Display omitted]</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>36623713</pmid><doi>10.1016/j.nano.2023.102651</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1549-9634 |
| ispartof | Nanomedicine, 2023-02, Vol.48, p.102651-102651, Article 102651 |
| issn | 1549-9634 1549-9642 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10492629 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Albumins Animals Cells, Cultured Chemical and Drug Induced Liver Injury - metabolism Drug Carriers - pharmacology Hepatocytes Hepatotoxicity In vitro culture Lipopolymer nanoparticle (LPN) Nanotoxicity Primary Cell Culture Primary rat hepatocyte Rats Urea - metabolism Urea - pharmacology |
| title | Evaluation of drug carrier hepatotoxicity using primary cell culture models |
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