Bacterial Pesticidal Protein Mpp51Aa1 Delivered via Transgenic Citrus Severely Impacts the Fecundity of Asian Citrus Psyllid, Diaphorina citri
The Asian citrus psyllid (ACP) Diaphorina citri vectors the causative agent of citrus greening disease that has the capacity to decimate citrus production. As an alternative and more sustainable approach to manage D. citri than repeated application of chemical insecticides, we investigated the poten...
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creator | Mishra, Ruchir Narayana, Ravishankar Ibanez, Freddy Achor, Diann Shilts, Turksen El-Mohtar, Choaa Orbović, Vladimir Stelinski, Lukasz L Bonning, Bryony C |
description | The Asian citrus psyllid (ACP) Diaphorina citri vectors the causative agent of citrus greening disease that has the capacity to decimate citrus production. As an alternative and more sustainable approach to manage D. citri than repeated application of chemical insecticides, we investigated the potential use of the bacteria-derived pesticidal protein, Mpp51Aa1, when delivered by transgenic Citrus sinensis cv. Valencia sweet orange or Citrus paradisi cv. Duncan grapefruit. Following confirmation of transcription and translation of
by transgenic plants, no impact of Mpp51Aa1 expression was seen on D. citri host plant choice between transgenic and control Duncan grapefruit plants. A slight but significant drop in survival of adult psyllids fed on these transgenic plants was noted relative to those fed on control plants. In line with this result, damage to the gut epithelium consistent with that caused by pore-forming proteins was only observed in a minority of adult D. citri fed on the transgenic Duncan grapefruit. However, greater impacts were observed on nymphs than on adults, with a 40% drop in the survival of nymphs fed on transgenic Duncan grapefruit relative to those fed on control plants. For Valencia sweet orange, a 70% decrease in the number of eggs laid by adult D. citri on transgenic plants was noted relative to those on control plants, with a 90% drop in emergence of progeny. These impacts that contrast with those associated with other bacterial pesticidal proteins and the potential for use of Mpp51Aa1-expressing transgenic plants for suppression of D. citri populations are discussed.
Pesticidal proteins derived from bacteria such as Bacillus thuringiensis are valuable tools for management of agricultural insect pests and provide a sustainable alternative to the application of chemical insecticides. However, relatively few bacterial pesticidal proteins have been used for suppression of hemipteran or sap-sucking insects such as the Asian citrus psyllid, Diaphorina citri. This insect is particularly important as the vector of the causative agent of citrus greening, or huanglongbing disease, which severely impacts global citrus production. In this study, we investigated the potential of transgenic citrus plants that produce the pesticidal protein Mpp51Aa1. While adult psyllid mortality on transgenic plants was modest, the reduced number of eggs laid by exposed adults and the decreased survival of progeny was such that psyllid populations dropped by mo |
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by transgenic plants, no impact of Mpp51Aa1 expression was seen on D. citri host plant choice between transgenic and control Duncan grapefruit plants. A slight but significant drop in survival of adult psyllids fed on these transgenic plants was noted relative to those fed on control plants. In line with this result, damage to the gut epithelium consistent with that caused by pore-forming proteins was only observed in a minority of adult D. citri fed on the transgenic Duncan grapefruit. However, greater impacts were observed on nymphs than on adults, with a 40% drop in the survival of nymphs fed on transgenic Duncan grapefruit relative to those fed on control plants. For Valencia sweet orange, a 70% decrease in the number of eggs laid by adult D. citri on transgenic plants was noted relative to those on control plants, with a 90% drop in emergence of progeny. These impacts that contrast with those associated with other bacterial pesticidal proteins and the potential for use of Mpp51Aa1-expressing transgenic plants for suppression of D. citri populations are discussed.
Pesticidal proteins derived from bacteria such as Bacillus thuringiensis are valuable tools for management of agricultural insect pests and provide a sustainable alternative to the application of chemical insecticides. However, relatively few bacterial pesticidal proteins have been used for suppression of hemipteran or sap-sucking insects such as the Asian citrus psyllid, Diaphorina citri. This insect is particularly important as the vector of the causative agent of citrus greening, or huanglongbing disease, which severely impacts global citrus production. In this study, we investigated the potential of transgenic citrus plants that produce the pesticidal protein Mpp51Aa1. While adult psyllid mortality on transgenic plants was modest, the reduced number of eggs laid by exposed adults and the decreased survival of progeny was such that psyllid populations dropped by more than 90%. These results provide valuable insight for potential deployment of Mpp51Aa1 in combination with other control agents for the management of D. citri.</description><identifier>ISSN: 0099-2240</identifier><identifier>ISSN: 1098-5336</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.00723-23</identifier><identifier>PMID: 37458593</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>Animals ; Bacteria ; Bacterial diseases ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biotechnology ; Citrus - microbiology ; Citrus fruits ; Citrus greening ; Citrus paradisi ; Citrus sinensis ; Diaphorina citri ; Epithelium ; Fecundity ; Fertility ; Fruits ; Grapefruit ; Hemiptera - genetics ; Hemiptera - microbiology ; Host plants ; Insecticides ; Insecticides - metabolism ; Insecticides - pharmacology ; Oranges ; Pesticides ; Pesticides - metabolism ; Plant bacterial diseases ; Plant diseases ; Plant Diseases - microbiology ; Plant Diseases - prevention & control ; Pore formation ; Pore-forming proteins ; Proteins ; Survival ; Transgenic plants ; Vectors</subject><ispartof>Applied and environmental microbiology, 2023-08, Vol.89 (8), p.e0072323</ispartof><rights>Copyright American Society for Microbiology Aug 2023</rights><rights>Copyright © 2023 Mishra et al. 2023 Mishra et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c370t-bd5d46e3e4884a8c254df45519d360be57eb5ae47797b6049ae745254a53a69c3</cites><orcidid>0000-0002-9956-9613</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10467345/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10467345/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,3189,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37458593$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Nikel, Pablo Ivan</contributor><creatorcontrib>Mishra, Ruchir</creatorcontrib><creatorcontrib>Narayana, Ravishankar</creatorcontrib><creatorcontrib>Ibanez, Freddy</creatorcontrib><creatorcontrib>Achor, Diann</creatorcontrib><creatorcontrib>Shilts, Turksen</creatorcontrib><creatorcontrib>El-Mohtar, Choaa</creatorcontrib><creatorcontrib>Orbović, Vladimir</creatorcontrib><creatorcontrib>Stelinski, Lukasz L</creatorcontrib><creatorcontrib>Bonning, Bryony C</creatorcontrib><title>Bacterial Pesticidal Protein Mpp51Aa1 Delivered via Transgenic Citrus Severely Impacts the Fecundity of Asian Citrus Psyllid, Diaphorina citri</title><title>Applied and environmental microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The Asian citrus psyllid (ACP) Diaphorina citri vectors the causative agent of citrus greening disease that has the capacity to decimate citrus production. As an alternative and more sustainable approach to manage D. citri than repeated application of chemical insecticides, we investigated the potential use of the bacteria-derived pesticidal protein, Mpp51Aa1, when delivered by transgenic Citrus sinensis cv. Valencia sweet orange or Citrus paradisi cv. Duncan grapefruit. Following confirmation of transcription and translation of
by transgenic plants, no impact of Mpp51Aa1 expression was seen on D. citri host plant choice between transgenic and control Duncan grapefruit plants. A slight but significant drop in survival of adult psyllids fed on these transgenic plants was noted relative to those fed on control plants. In line with this result, damage to the gut epithelium consistent with that caused by pore-forming proteins was only observed in a minority of adult D. citri fed on the transgenic Duncan grapefruit. However, greater impacts were observed on nymphs than on adults, with a 40% drop in the survival of nymphs fed on transgenic Duncan grapefruit relative to those fed on control plants. For Valencia sweet orange, a 70% decrease in the number of eggs laid by adult D. citri on transgenic plants was noted relative to those on control plants, with a 90% drop in emergence of progeny. These impacts that contrast with those associated with other bacterial pesticidal proteins and the potential for use of Mpp51Aa1-expressing transgenic plants for suppression of D. citri populations are discussed.
Pesticidal proteins derived from bacteria such as Bacillus thuringiensis are valuable tools for management of agricultural insect pests and provide a sustainable alternative to the application of chemical insecticides. However, relatively few bacterial pesticidal proteins have been used for suppression of hemipteran or sap-sucking insects such as the Asian citrus psyllid, Diaphorina citri. This insect is particularly important as the vector of the causative agent of citrus greening, or huanglongbing disease, which severely impacts global citrus production. In this study, we investigated the potential of transgenic citrus plants that produce the pesticidal protein Mpp51Aa1. While adult psyllid mortality on transgenic plants was modest, the reduced number of eggs laid by exposed adults and the decreased survival of progeny was such that psyllid populations dropped by more than 90%. These results provide valuable insight for potential deployment of Mpp51Aa1 in combination with other control agents for the management of D. citri.</description><subject>Animals</subject><subject>Bacteria</subject><subject>Bacterial diseases</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biotechnology</subject><subject>Citrus - microbiology</subject><subject>Citrus fruits</subject><subject>Citrus greening</subject><subject>Citrus paradisi</subject><subject>Citrus sinensis</subject><subject>Diaphorina citri</subject><subject>Epithelium</subject><subject>Fecundity</subject><subject>Fertility</subject><subject>Fruits</subject><subject>Grapefruit</subject><subject>Hemiptera - genetics</subject><subject>Hemiptera - microbiology</subject><subject>Host plants</subject><subject>Insecticides</subject><subject>Insecticides - metabolism</subject><subject>Insecticides - pharmacology</subject><subject>Oranges</subject><subject>Pesticides</subject><subject>Pesticides - metabolism</subject><subject>Plant bacterial diseases</subject><subject>Plant diseases</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Diseases - prevention & control</subject><subject>Pore formation</subject><subject>Pore-forming proteins</subject><subject>Proteins</subject><subject>Survival</subject><subject>Transgenic plants</subject><subject>Vectors</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU9v1DAQxS0EokvhxhlZ4tJD09qxncQntGwpVCqiEuVsTZzZrqvEDnay0n6Jfma89I-gpxnp_fQ0bx4h7zk74bxsTgGHE8bqUhSleEEWnOmmUEJUL8mCMa2LspTsgLxJ6ZYxJlnVvCYHopaqUVosyN1nsBNGBz29wjQ567r9GsOEztPv46j4Ejg9w95tMWJHtw7odQSfbtA7S1duinOiP3Gv9jt6MYzZMNFpg_Qc7ew7N-1oWNNlcuAf8au063vXHdMzB-MmROeB2iy5t-TVGvqE7x7mIfl1_uV69a24_PH1YrW8LKyo2VS0nepkhQJl00hobKlkt5ZKcd2JirWoamwVoKxrXbcVkxowJ84UKAGVtuKQfLr3Hed2wM6inyL0ZoxugLgzAZz5X_FuY27C1nAmq1pIlR2OHhxi-D3n15nBJYt9Dx7DnEzZCF3KWlRVRj8-Q2_DHH3OlymlM9Eonqnje8rGkFLE9dM1nJl90yY3bf42bUqR8Q__JniCH6sVfwCMwqW4</recordid><startdate>20230830</startdate><enddate>20230830</enddate><creator>Mishra, Ruchir</creator><creator>Narayana, Ravishankar</creator><creator>Ibanez, Freddy</creator><creator>Achor, Diann</creator><creator>Shilts, Turksen</creator><creator>El-Mohtar, Choaa</creator><creator>Orbović, Vladimir</creator><creator>Stelinski, Lukasz L</creator><creator>Bonning, Bryony C</creator><general>American Society for Microbiology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-9956-9613</orcidid></search><sort><creationdate>20230830</creationdate><title>Bacterial Pesticidal Protein Mpp51Aa1 Delivered via Transgenic Citrus Severely Impacts the Fecundity of Asian Citrus Psyllid, Diaphorina citri</title><author>Mishra, Ruchir ; Narayana, Ravishankar ; Ibanez, Freddy ; Achor, Diann ; Shilts, Turksen ; El-Mohtar, Choaa ; Orbović, Vladimir ; Stelinski, Lukasz L ; Bonning, Bryony C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-bd5d46e3e4884a8c254df45519d360be57eb5ae47797b6049ae745254a53a69c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Bacterial diseases</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biotechnology</topic><topic>Citrus - microbiology</topic><topic>Citrus fruits</topic><topic>Citrus greening</topic><topic>Citrus paradisi</topic><topic>Citrus sinensis</topic><topic>Diaphorina citri</topic><topic>Epithelium</topic><topic>Fecundity</topic><topic>Fertility</topic><topic>Fruits</topic><topic>Grapefruit</topic><topic>Hemiptera - genetics</topic><topic>Hemiptera - microbiology</topic><topic>Host plants</topic><topic>Insecticides</topic><topic>Insecticides - metabolism</topic><topic>Insecticides - pharmacology</topic><topic>Oranges</topic><topic>Pesticides</topic><topic>Pesticides - metabolism</topic><topic>Plant bacterial diseases</topic><topic>Plant diseases</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Diseases - prevention & control</topic><topic>Pore formation</topic><topic>Pore-forming proteins</topic><topic>Proteins</topic><topic>Survival</topic><topic>Transgenic plants</topic><topic>Vectors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mishra, Ruchir</creatorcontrib><creatorcontrib>Narayana, Ravishankar</creatorcontrib><creatorcontrib>Ibanez, Freddy</creatorcontrib><creatorcontrib>Achor, Diann</creatorcontrib><creatorcontrib>Shilts, Turksen</creatorcontrib><creatorcontrib>El-Mohtar, Choaa</creatorcontrib><creatorcontrib>Orbović, Vladimir</creatorcontrib><creatorcontrib>Stelinski, Lukasz L</creatorcontrib><creatorcontrib>Bonning, Bryony C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mishra, Ruchir</au><au>Narayana, Ravishankar</au><au>Ibanez, Freddy</au><au>Achor, Diann</au><au>Shilts, Turksen</au><au>El-Mohtar, Choaa</au><au>Orbović, Vladimir</au><au>Stelinski, Lukasz L</au><au>Bonning, Bryony C</au><au>Nikel, Pablo Ivan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial Pesticidal Protein Mpp51Aa1 Delivered via Transgenic Citrus Severely Impacts the Fecundity of Asian Citrus Psyllid, Diaphorina citri</atitle><jtitle>Applied and environmental microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2023-08-30</date><risdate>2023</risdate><volume>89</volume><issue>8</issue><spage>e0072323</spage><pages>e0072323-</pages><issn>0099-2240</issn><issn>1098-5336</issn><eissn>1098-5336</eissn><abstract>The Asian citrus psyllid (ACP) Diaphorina citri vectors the causative agent of citrus greening disease that has the capacity to decimate citrus production. As an alternative and more sustainable approach to manage D. citri than repeated application of chemical insecticides, we investigated the potential use of the bacteria-derived pesticidal protein, Mpp51Aa1, when delivered by transgenic Citrus sinensis cv. Valencia sweet orange or Citrus paradisi cv. Duncan grapefruit. Following confirmation of transcription and translation of
by transgenic plants, no impact of Mpp51Aa1 expression was seen on D. citri host plant choice between transgenic and control Duncan grapefruit plants. A slight but significant drop in survival of adult psyllids fed on these transgenic plants was noted relative to those fed on control plants. In line with this result, damage to the gut epithelium consistent with that caused by pore-forming proteins was only observed in a minority of adult D. citri fed on the transgenic Duncan grapefruit. However, greater impacts were observed on nymphs than on adults, with a 40% drop in the survival of nymphs fed on transgenic Duncan grapefruit relative to those fed on control plants. For Valencia sweet orange, a 70% decrease in the number of eggs laid by adult D. citri on transgenic plants was noted relative to those on control plants, with a 90% drop in emergence of progeny. These impacts that contrast with those associated with other bacterial pesticidal proteins and the potential for use of Mpp51Aa1-expressing transgenic plants for suppression of D. citri populations are discussed.
Pesticidal proteins derived from bacteria such as Bacillus thuringiensis are valuable tools for management of agricultural insect pests and provide a sustainable alternative to the application of chemical insecticides. However, relatively few bacterial pesticidal proteins have been used for suppression of hemipteran or sap-sucking insects such as the Asian citrus psyllid, Diaphorina citri. This insect is particularly important as the vector of the causative agent of citrus greening, or huanglongbing disease, which severely impacts global citrus production. In this study, we investigated the potential of transgenic citrus plants that produce the pesticidal protein Mpp51Aa1. While adult psyllid mortality on transgenic plants was modest, the reduced number of eggs laid by exposed adults and the decreased survival of progeny was such that psyllid populations dropped by more than 90%. These results provide valuable insight for potential deployment of Mpp51Aa1 in combination with other control agents for the management of D. citri.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>37458593</pmid><doi>10.1128/aem.00723-23</doi><orcidid>https://orcid.org/0000-0002-9956-9613</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Animals Bacteria Bacterial diseases Bacterial Proteins - genetics Bacterial Proteins - metabolism Biotechnology Citrus - microbiology Citrus fruits Citrus greening Citrus paradisi Citrus sinensis Diaphorina citri Epithelium Fecundity Fertility Fruits Grapefruit Hemiptera - genetics Hemiptera - microbiology Host plants Insecticides Insecticides - metabolism Insecticides - pharmacology Oranges Pesticides Pesticides - metabolism Plant bacterial diseases Plant diseases Plant Diseases - microbiology Plant Diseases - prevention & control Pore formation Pore-forming proteins Proteins Survival Transgenic plants Vectors |
title | Bacterial Pesticidal Protein Mpp51Aa1 Delivered via Transgenic Citrus Severely Impacts the Fecundity of Asian Citrus Psyllid, Diaphorina citri |
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