Tick-Borne Pathogens Screening Using a Multiplex Real-Time Polymerase Chain Reaction-Based Method
Purpose This study aims to develop and evaluate a cost-effective, user-friendly multiplex quantitative real-time polymerase chain reaction (qPCR) method for detecting multiple tick-borne pathogens associated with human and veterinary diseases. Methods In silico PCR was performed to design and evalua...
Gespeichert in:
| Veröffentlicht in: | Acta parasitologica 2023-09, Vol.68 (3), p.705-710 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 710 |
|---|---|
| container_issue | 3 |
| container_start_page | 705 |
| container_title | Acta parasitologica |
| container_volume | 68 |
| creator | Cardenas-Cadena, Sergio Andres Castañeda-Lopez, Maria Eugenia Mollinedo-Montaño, Fabiana Esther Vazquez-Reyes, Sodel Lara-Arias, Jorge Marino-Martinez, Ivan Alberto Rodriguez-Sanchez, Iram Pablo Garza-Veloz, Idalia Martinez-Fierro, Margarita L. |
| description | Purpose
This study aims to develop and evaluate a cost-effective, user-friendly multiplex quantitative real-time polymerase chain reaction (qPCR) method for detecting multiple tick-borne pathogens associated with human and veterinary diseases.
Methods
In silico PCR was performed to design and evaluate primer sequences reported for amplifying
Rickettsia spp
.,
Borrelia spp.
, and
Ehrlichia spp.
Single and multiplex qPCR assays were then standardized to detect individual pathogens and multiple pathogens in a single reaction. Positive controls were generated to determine the dynamic range of the methods. In the validation phase, a total of 800 samples were screened for the presence of tick-borne pathogens.
Results
Identification in a single qPCR reaction (multiplex) of
Ehrlichia spp
.
, and
Borrelia spp.
with a limit of detection of 10 copies and
Rickettsia spp
. with 100 copies, a PCR efficiency (E) of 90–100% and a coefficient of correlation (
R
2
) of 0.998–0.996 for all pathogens.
Conclusion
The ability to detect three significant pathogens
(Ehrlichia spp
.,
Rickettsia spp
., and
Borrelia spp
.) in a single qPCR reaction offers a significant advantage in the field of molecular diagnostics for tick-borne diseases. This advancement has a profound impact on public health as it facilitates the selection of appropriate treatment protocols, thereby reducing complications associated with disease progression. The streamlined approach provided by this method simplifies the diagnostic process and enables timely intervention, ultimately improving patient outcomes and mitigating the potential risks associated with untreated or misdiagnosed tick-borne infections. |
| doi_str_mv | 10.1007/s11686-023-00702-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10462521</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2858380988</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-931789437b10952aaed6e8c5fba7b889fe1c6a490443c64f596c7adfb7e952373</originalsourceid><addsrcrecordid>eNp9kUtv1DAURi0Eog_4AyyqSGzYGPyIY3tV0RFtkVqBYLq2HOdmxm1iD3aC2n9fD1MKZcHGr3t8_PgQekPJe0qI_JApbVSDCeO4TAnD5Bnap0o3mCpBn5cx4wQzxegeOsj5mpC6UUq9RHtcCl4Ueh_ZpXc3-CSmANVXO63jCkKuvrsEEHxYVVd529rqch4mvxngtvoGdsBLPxY-DncjJJuhWqytD9uSm3wM-KSsddUlFF_3Cr3o7ZDh9UN_iK5OPy0X5_jiy9nnxccL7GopJqw5lUrXXLaUaMGsha4B5UTfWtkqpXugrrG1JnXNXVP3QjdO2q5vJRScS36IjnfezdyO0DkIU7KD2SQ_2nRnovXmaSX4tVnFn4aWb2GC0WJ492BI8ccMeTKjzw6GwQaIczZM1YISwQgp6Nt_0Os4p1DeVyihuCJaqUKxHeVSzDlB_3gbSsw2QrOL0JQIza8IzVZ99Pc7Hrf8zqwAfAfkUgorSH_O_o_2HtGEpto</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2858380988</pqid></control><display><type>article</type><title>Tick-Borne Pathogens Screening Using a Multiplex Real-Time Polymerase Chain Reaction-Based Method</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Cardenas-Cadena, Sergio Andres ; Castañeda-Lopez, Maria Eugenia ; Mollinedo-Montaño, Fabiana Esther ; Vazquez-Reyes, Sodel ; Lara-Arias, Jorge ; Marino-Martinez, Ivan Alberto ; Rodriguez-Sanchez, Iram Pablo ; Garza-Veloz, Idalia ; Martinez-Fierro, Margarita L.</creator><creatorcontrib>Cardenas-Cadena, Sergio Andres ; Castañeda-Lopez, Maria Eugenia ; Mollinedo-Montaño, Fabiana Esther ; Vazquez-Reyes, Sodel ; Lara-Arias, Jorge ; Marino-Martinez, Ivan Alberto ; Rodriguez-Sanchez, Iram Pablo ; Garza-Veloz, Idalia ; Martinez-Fierro, Margarita L.</creatorcontrib><description>Purpose
This study aims to develop and evaluate a cost-effective, user-friendly multiplex quantitative real-time polymerase chain reaction (qPCR) method for detecting multiple tick-borne pathogens associated with human and veterinary diseases.
Methods
In silico PCR was performed to design and evaluate primer sequences reported for amplifying
Rickettsia spp
.,
Borrelia spp.
, and
Ehrlichia spp.
Single and multiplex qPCR assays were then standardized to detect individual pathogens and multiple pathogens in a single reaction. Positive controls were generated to determine the dynamic range of the methods. In the validation phase, a total of 800 samples were screened for the presence of tick-borne pathogens.
Results
Identification in a single qPCR reaction (multiplex) of
Ehrlichia spp
.
, and
Borrelia spp.
with a limit of detection of 10 copies and
Rickettsia spp
. with 100 copies, a PCR efficiency (E) of 90–100% and a coefficient of correlation (
R
2
) of 0.998–0.996 for all pathogens.
Conclusion
The ability to detect three significant pathogens
(Ehrlichia spp
.,
Rickettsia spp
., and
Borrelia spp
.) in a single qPCR reaction offers a significant advantage in the field of molecular diagnostics for tick-borne diseases. This advancement has a profound impact on public health as it facilitates the selection of appropriate treatment protocols, thereby reducing complications associated with disease progression. The streamlined approach provided by this method simplifies the diagnostic process and enables timely intervention, ultimately improving patient outcomes and mitigating the potential risks associated with untreated or misdiagnosed tick-borne infections.</description><identifier>ISSN: 1230-2821</identifier><identifier>EISSN: 1896-1851</identifier><identifier>DOI: 10.1007/s11686-023-00702-0</identifier><identifier>PMID: 37531009</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animal Systematics/Taxonomy/Biogeography ; Animals ; Arachnids ; Biomedical and Life Sciences ; Biomedicine ; Borrelia ; Borrelia - genetics ; Ecology ; Ehrlichia ; Ehrlichia - genetics ; Humans ; Medical Microbiology ; Microbiology ; Multiplexing ; Parasitic diseases ; Parasitology ; Pathogens ; Polymerase chain reaction ; Public health ; Real time ; Real-Time Polymerase Chain Reaction ; Rickettsia ; Rickettsia - genetics ; Short Communication ; Tick-borne diseases ; Tick-Borne Diseases - diagnosis ; Tick-Borne Diseases - veterinary ; Ticks - microbiology ; Veterinary diseases</subject><ispartof>Acta parasitologica, 2023-09, Vol.68 (3), p.705-710</ispartof><rights>The Author(s) 2023. corrected publication 2024</rights><rights>2023. The Author(s).</rights><rights>The Author(s) 2023. corrected publication 2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-931789437b10952aaed6e8c5fba7b889fe1c6a490443c64f596c7adfb7e952373</citedby><cites>FETCH-LOGICAL-c475t-931789437b10952aaed6e8c5fba7b889fe1c6a490443c64f596c7adfb7e952373</cites><orcidid>0000-0003-1478-9068</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11686-023-00702-0$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11686-023-00702-0$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37531009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cardenas-Cadena, Sergio Andres</creatorcontrib><creatorcontrib>Castañeda-Lopez, Maria Eugenia</creatorcontrib><creatorcontrib>Mollinedo-Montaño, Fabiana Esther</creatorcontrib><creatorcontrib>Vazquez-Reyes, Sodel</creatorcontrib><creatorcontrib>Lara-Arias, Jorge</creatorcontrib><creatorcontrib>Marino-Martinez, Ivan Alberto</creatorcontrib><creatorcontrib>Rodriguez-Sanchez, Iram Pablo</creatorcontrib><creatorcontrib>Garza-Veloz, Idalia</creatorcontrib><creatorcontrib>Martinez-Fierro, Margarita L.</creatorcontrib><title>Tick-Borne Pathogens Screening Using a Multiplex Real-Time Polymerase Chain Reaction-Based Method</title><title>Acta parasitologica</title><addtitle>Acta Parasit</addtitle><addtitle>Acta Parasitol</addtitle><description>Purpose
This study aims to develop and evaluate a cost-effective, user-friendly multiplex quantitative real-time polymerase chain reaction (qPCR) method for detecting multiple tick-borne pathogens associated with human and veterinary diseases.
Methods
In silico PCR was performed to design and evaluate primer sequences reported for amplifying
Rickettsia spp
.,
Borrelia spp.
, and
Ehrlichia spp.
Single and multiplex qPCR assays were then standardized to detect individual pathogens and multiple pathogens in a single reaction. Positive controls were generated to determine the dynamic range of the methods. In the validation phase, a total of 800 samples were screened for the presence of tick-borne pathogens.
Results
Identification in a single qPCR reaction (multiplex) of
Ehrlichia spp
.
, and
Borrelia spp.
with a limit of detection of 10 copies and
Rickettsia spp
. with 100 copies, a PCR efficiency (E) of 90–100% and a coefficient of correlation (
R
2
) of 0.998–0.996 for all pathogens.
Conclusion
The ability to detect three significant pathogens
(Ehrlichia spp
.,
Rickettsia spp
., and
Borrelia spp
.) in a single qPCR reaction offers a significant advantage in the field of molecular diagnostics for tick-borne diseases. This advancement has a profound impact on public health as it facilitates the selection of appropriate treatment protocols, thereby reducing complications associated with disease progression. The streamlined approach provided by this method simplifies the diagnostic process and enables timely intervention, ultimately improving patient outcomes and mitigating the potential risks associated with untreated or misdiagnosed tick-borne infections.</description><subject>Animal Systematics/Taxonomy/Biogeography</subject><subject>Animals</subject><subject>Arachnids</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Borrelia</subject><subject>Borrelia - genetics</subject><subject>Ecology</subject><subject>Ehrlichia</subject><subject>Ehrlichia - genetics</subject><subject>Humans</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Multiplexing</subject><subject>Parasitic diseases</subject><subject>Parasitology</subject><subject>Pathogens</subject><subject>Polymerase chain reaction</subject><subject>Public health</subject><subject>Real time</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Rickettsia</subject><subject>Rickettsia - genetics</subject><subject>Short Communication</subject><subject>Tick-borne diseases</subject><subject>Tick-Borne Diseases - diagnosis</subject><subject>Tick-Borne Diseases - veterinary</subject><subject>Ticks - microbiology</subject><subject>Veterinary diseases</subject><issn>1230-2821</issn><issn>1896-1851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAURi0Eog_4AyyqSGzYGPyIY3tV0RFtkVqBYLq2HOdmxm1iD3aC2n9fD1MKZcHGr3t8_PgQekPJe0qI_JApbVSDCeO4TAnD5Bnap0o3mCpBn5cx4wQzxegeOsj5mpC6UUq9RHtcCl4Ueh_ZpXc3-CSmANVXO63jCkKuvrsEEHxYVVd529rqch4mvxngtvoGdsBLPxY-DncjJJuhWqytD9uSm3wM-KSsddUlFF_3Cr3o7ZDh9UN_iK5OPy0X5_jiy9nnxccL7GopJqw5lUrXXLaUaMGsha4B5UTfWtkqpXugrrG1JnXNXVP3QjdO2q5vJRScS36IjnfezdyO0DkIU7KD2SQ_2nRnovXmaSX4tVnFn4aWb2GC0WJ492BI8ccMeTKjzw6GwQaIczZM1YISwQgp6Nt_0Os4p1DeVyihuCJaqUKxHeVSzDlB_3gbSsw2QrOL0JQIza8IzVZ99Pc7Hrf8zqwAfAfkUgorSH_O_o_2HtGEpto</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Cardenas-Cadena, Sergio Andres</creator><creator>Castañeda-Lopez, Maria Eugenia</creator><creator>Mollinedo-Montaño, Fabiana Esther</creator><creator>Vazquez-Reyes, Sodel</creator><creator>Lara-Arias, Jorge</creator><creator>Marino-Martinez, Ivan Alberto</creator><creator>Rodriguez-Sanchez, Iram Pablo</creator><creator>Garza-Veloz, Idalia</creator><creator>Martinez-Fierro, Margarita L.</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-1478-9068</orcidid></search><sort><creationdate>20230901</creationdate><title>Tick-Borne Pathogens Screening Using a Multiplex Real-Time Polymerase Chain Reaction-Based Method</title><author>Cardenas-Cadena, Sergio Andres ; Castañeda-Lopez, Maria Eugenia ; Mollinedo-Montaño, Fabiana Esther ; Vazquez-Reyes, Sodel ; Lara-Arias, Jorge ; Marino-Martinez, Ivan Alberto ; Rodriguez-Sanchez, Iram Pablo ; Garza-Veloz, Idalia ; Martinez-Fierro, Margarita L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-931789437b10952aaed6e8c5fba7b889fe1c6a490443c64f596c7adfb7e952373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animal Systematics/Taxonomy/Biogeography</topic><topic>Animals</topic><topic>Arachnids</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Borrelia</topic><topic>Borrelia - genetics</topic><topic>Ecology</topic><topic>Ehrlichia</topic><topic>Ehrlichia - genetics</topic><topic>Humans</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Multiplexing</topic><topic>Parasitic diseases</topic><topic>Parasitology</topic><topic>Pathogens</topic><topic>Polymerase chain reaction</topic><topic>Public health</topic><topic>Real time</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Rickettsia</topic><topic>Rickettsia - genetics</topic><topic>Short Communication</topic><topic>Tick-borne diseases</topic><topic>Tick-Borne Diseases - diagnosis</topic><topic>Tick-Borne Diseases - veterinary</topic><topic>Ticks - microbiology</topic><topic>Veterinary diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cardenas-Cadena, Sergio Andres</creatorcontrib><creatorcontrib>Castañeda-Lopez, Maria Eugenia</creatorcontrib><creatorcontrib>Mollinedo-Montaño, Fabiana Esther</creatorcontrib><creatorcontrib>Vazquez-Reyes, Sodel</creatorcontrib><creatorcontrib>Lara-Arias, Jorge</creatorcontrib><creatorcontrib>Marino-Martinez, Ivan Alberto</creatorcontrib><creatorcontrib>Rodriguez-Sanchez, Iram Pablo</creatorcontrib><creatorcontrib>Garza-Veloz, Idalia</creatorcontrib><creatorcontrib>Martinez-Fierro, Margarita L.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta parasitologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cardenas-Cadena, Sergio Andres</au><au>Castañeda-Lopez, Maria Eugenia</au><au>Mollinedo-Montaño, Fabiana Esther</au><au>Vazquez-Reyes, Sodel</au><au>Lara-Arias, Jorge</au><au>Marino-Martinez, Ivan Alberto</au><au>Rodriguez-Sanchez, Iram Pablo</au><au>Garza-Veloz, Idalia</au><au>Martinez-Fierro, Margarita L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tick-Borne Pathogens Screening Using a Multiplex Real-Time Polymerase Chain Reaction-Based Method</atitle><jtitle>Acta parasitologica</jtitle><stitle>Acta Parasit</stitle><addtitle>Acta Parasitol</addtitle><date>2023-09-01</date><risdate>2023</risdate><volume>68</volume><issue>3</issue><spage>705</spage><epage>710</epage><pages>705-710</pages><issn>1230-2821</issn><eissn>1896-1851</eissn><abstract>Purpose
This study aims to develop and evaluate a cost-effective, user-friendly multiplex quantitative real-time polymerase chain reaction (qPCR) method for detecting multiple tick-borne pathogens associated with human and veterinary diseases.
Methods
In silico PCR was performed to design and evaluate primer sequences reported for amplifying
Rickettsia spp
.,
Borrelia spp.
, and
Ehrlichia spp.
Single and multiplex qPCR assays were then standardized to detect individual pathogens and multiple pathogens in a single reaction. Positive controls were generated to determine the dynamic range of the methods. In the validation phase, a total of 800 samples were screened for the presence of tick-borne pathogens.
Results
Identification in a single qPCR reaction (multiplex) of
Ehrlichia spp
.
, and
Borrelia spp.
with a limit of detection of 10 copies and
Rickettsia spp
. with 100 copies, a PCR efficiency (E) of 90–100% and a coefficient of correlation (
R
2
) of 0.998–0.996 for all pathogens.
Conclusion
The ability to detect three significant pathogens
(Ehrlichia spp
.,
Rickettsia spp
., and
Borrelia spp
.) in a single qPCR reaction offers a significant advantage in the field of molecular diagnostics for tick-borne diseases. This advancement has a profound impact on public health as it facilitates the selection of appropriate treatment protocols, thereby reducing complications associated with disease progression. The streamlined approach provided by this method simplifies the diagnostic process and enables timely intervention, ultimately improving patient outcomes and mitigating the potential risks associated with untreated or misdiagnosed tick-borne infections.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>37531009</pmid><doi>10.1007/s11686-023-00702-0</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-1478-9068</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1230-2821 |
| ispartof | Acta parasitologica, 2023-09, Vol.68 (3), p.705-710 |
| issn | 1230-2821 1896-1851 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10462521 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animal Systematics/Taxonomy/Biogeography Animals Arachnids Biomedical and Life Sciences Biomedicine Borrelia Borrelia - genetics Ecology Ehrlichia Ehrlichia - genetics Humans Medical Microbiology Microbiology Multiplexing Parasitic diseases Parasitology Pathogens Polymerase chain reaction Public health Real time Real-Time Polymerase Chain Reaction Rickettsia Rickettsia - genetics Short Communication Tick-borne diseases Tick-Borne Diseases - diagnosis Tick-Borne Diseases - veterinary Ticks - microbiology Veterinary diseases |
| title | Tick-Borne Pathogens Screening Using a Multiplex Real-Time Polymerase Chain Reaction-Based Method |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T02%3A04%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tick-Borne%20Pathogens%20Screening%20Using%20a%20Multiplex%20Real-Time%20Polymerase%20Chain%20Reaction-Based%20Method&rft.jtitle=Acta%20parasitologica&rft.au=Cardenas-Cadena,%20Sergio%20Andres&rft.date=2023-09-01&rft.volume=68&rft.issue=3&rft.spage=705&rft.epage=710&rft.pages=705-710&rft.issn=1230-2821&rft.eissn=1896-1851&rft_id=info:doi/10.1007/s11686-023-00702-0&rft_dat=%3Cproquest_pubme%3E2858380988%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2858380988&rft_id=info:pmid/37531009&rfr_iscdi=true |