Epidemiological study of a food-borne outbreak of enterotoxigenic Escherichia coli O25:NM by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA analysis
This study investigated the applicability of molecular epidemiological techniques to the identification of the causal agent of an outbreak of diarrhea caused by ingestion of food contaminated with enterotoxigenic Escherichia coli (ETEC). The outbreak occurred at four elementary schools in July 1996...
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| Veröffentlicht in: | Journal of clinical microbiology 1998-03, Vol.36 (3), p.652-656 |
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| container_title | Journal of clinical microbiology |
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| creator | Mitsuda, T Muto, T Yamada, M Kobayashi, N Toba, M Aihara, Y Ito, A Yokota, S |
| description | This study investigated the applicability of molecular epidemiological techniques to the identification of the causal agent of an outbreak of diarrhea caused by ingestion of food contaminated with enterotoxigenic Escherichia coli (ETEC). The outbreak occurred at four elementary schools in July 1996 and affected more than 800 people. Illness was most strongly associated with eating tuna paste (relative risk, 1.79; 95% confidence interval = 1.16 to 2.79; P = 0.0001). To evaluate the epidemiological characteristics of the pathogen, the DNAs from numerous isolated ETEC strains were subjected to randomly amplified polymorphic DNA analysis, pulsed-field gel electrophoresis of nuclease S1-treated plasmid DNA, and analysis of genomic DNA restriction fragment length polymorphisms. All ETEC isolates were of the O25:NM (nonmotile) serotype, which carries a heat-stable enterotoxin Ib gene. Genotypic analysis demonstrated that the strains isolated from the patients at all four schools were identical. The isolates of ETEC O25:NM obtained from the tuna paste that had been served for lunch at these schools were genetically indistinguishable from those isolated from the patients. Results suggest that this outbreak was food borne. The molecular biology-based epidemiological techniques used in this study were useful in characterizing the causal agent in this food-borne epidemic. |
| doi_str_mv | 10.1128/jcm.36.3.652-656.1998 |
| format | Article |
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The outbreak occurred at four elementary schools in July 1996 and affected more than 800 people. Illness was most strongly associated with eating tuna paste (relative risk, 1.79; 95% confidence interval = 1.16 to 2.79; P = 0.0001). To evaluate the epidemiological characteristics of the pathogen, the DNAs from numerous isolated ETEC strains were subjected to randomly amplified polymorphic DNA analysis, pulsed-field gel electrophoresis of nuclease S1-treated plasmid DNA, and analysis of genomic DNA restriction fragment length polymorphisms. All ETEC isolates were of the O25:NM (nonmotile) serotype, which carries a heat-stable enterotoxin Ib gene. Genotypic analysis demonstrated that the strains isolated from the patients at all four schools were identical. The isolates of ETEC O25:NM obtained from the tuna paste that had been served for lunch at these schools were genetically indistinguishable from those isolated from the patients. Results suggest that this outbreak was food borne. The molecular biology-based epidemiological techniques used in this study were useful in characterizing the causal agent in this food-borne epidemic.</description><identifier>ISSN: 0095-1137</identifier><identifier>EISSN: 1098-660X</identifier><identifier>DOI: 10.1128/jcm.36.3.652-656.1998</identifier><identifier>PMID: 9508290</identifier><identifier>CODEN: JCMIDW</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Bacterial Toxins - analysis ; Bacteriology ; Biological and medical sciences ; Disease Outbreaks ; DNA, Bacterial - genetics ; Electrophoresis, Gel, Pulsed-Field ; Enterotoxins - analysis ; Epidemiology ; Escherichia coli - genetics ; Escherichia coli - isolation & purification ; Escherichia coli Infections - epidemiology ; Escherichia coli Infections - microbiology ; fish paste ; Fish Products - microbiology ; Food Microbiology ; foodborne illness ; Fundamental and applied biological sciences. Psychology ; Genotype ; Human bacterial diseases ; Humans ; Infectious diseases ; Medical sciences ; Microbiology ; Molecular Epidemiology ; Phenotype ; Plasmids ; Polymorphism, Restriction Fragment Length ; Random Amplified Polymorphic DNA Technique ; Tuna - microbiology</subject><ispartof>Journal of clinical microbiology, 1998-03, Vol.36 (3), p.652-656</ispartof><rights>1998 INIST-CNRS</rights><rights>Copyright © 1998, American Society for Microbiology 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-b1bd3688936d2b7a081bb3c256ad215bc3a1abd8d650b1bd6a45e094be6d28283</citedby><cites>FETCH-LOGICAL-c489t-b1bd3688936d2b7a081bb3c256ad215bc3a1abd8d650b1bd6a45e094be6d28283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC104603/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC104603/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2204068$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9508290$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mitsuda, T</creatorcontrib><creatorcontrib>Muto, T</creatorcontrib><creatorcontrib>Yamada, M</creatorcontrib><creatorcontrib>Kobayashi, N</creatorcontrib><creatorcontrib>Toba, M</creatorcontrib><creatorcontrib>Aihara, Y</creatorcontrib><creatorcontrib>Ito, A</creatorcontrib><creatorcontrib>Yokota, S</creatorcontrib><title>Epidemiological study of a food-borne outbreak of enterotoxigenic Escherichia coli O25:NM by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA analysis</title><title>Journal of clinical microbiology</title><addtitle>J Clin Microbiol</addtitle><description>This study investigated the applicability of molecular epidemiological techniques to the identification of the causal agent of an outbreak of diarrhea caused by ingestion of food contaminated with enterotoxigenic Escherichia coli (ETEC). The outbreak occurred at four elementary schools in July 1996 and affected more than 800 people. Illness was most strongly associated with eating tuna paste (relative risk, 1.79; 95% confidence interval = 1.16 to 2.79; P = 0.0001). To evaluate the epidemiological characteristics of the pathogen, the DNAs from numerous isolated ETEC strains were subjected to randomly amplified polymorphic DNA analysis, pulsed-field gel electrophoresis of nuclease S1-treated plasmid DNA, and analysis of genomic DNA restriction fragment length polymorphisms. All ETEC isolates were of the O25:NM (nonmotile) serotype, which carries a heat-stable enterotoxin Ib gene. Genotypic analysis demonstrated that the strains isolated from the patients at all four schools were identical. The isolates of ETEC O25:NM obtained from the tuna paste that had been served for lunch at these schools were genetically indistinguishable from those isolated from the patients. Results suggest that this outbreak was food borne. The molecular biology-based epidemiological techniques used in this study were useful in characterizing the causal agent in this food-borne epidemic.</description><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Bacterial Toxins - analysis</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Disease Outbreaks</subject><subject>DNA, Bacterial - genetics</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Enterotoxins - analysis</subject><subject>Epidemiology</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli Infections - epidemiology</subject><subject>Escherichia coli Infections - microbiology</subject><subject>fish paste</subject><subject>Fish Products - microbiology</subject><subject>Food Microbiology</subject><subject>foodborne illness</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Molecular Epidemiology</subject><subject>Phenotype</subject><subject>Plasmids</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>Tuna - microbiology</subject><issn>0095-1137</issn><issn>1098-660X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksuO0zAYhSMEGsrAI4zwArFL8SV2bSQWo1JumssCRmJn-ZbWg1Nn7ASRh-IdcdSqghUL24vznaP_l09VXSC4RAjzN_emWxK2JEtGcc0oWyIh-KNqgaDgNWPw--NqAaGgNUJk9bR6lvM9hKhpKD2rzgSFHAu4qH5vem9d52OIW29UAHkY7QRiCxRoY7S1jmnvQBwHnZz6MQtuP7gUh_jLb93eG7DJZueSNzuvgInBg1tM395cAz2BfgzZ2br1LliwdQG44MyQYr-LyWWfgdpbkMoVuzAB1fXBF9aCPoapi6nflfj3N5cFU2Eq_PPqSatK5Ivje17dfdh8W3-qr24_fl5fXtWm4WKoNdKWMM4FYRbrlYIcaU0MpkxZjKg2RCGlLbeMwpllqqEOika7wnPMyXn17pDbj7pz1pSVkwqyT75TaZJRefmvsvc7uY0_JYINg6T4Xx_9KT6MLg-y89m4ENTexTHLlVhhjsp8_wMRowQLOoP0AJoUc06uPQ2DoJz7IL-sryVhksjSh3KYnPtQfBd_b3JyHQtQ9FdHXeXy_W35DOPzCcMYNpDNMS8PWKuiVNtUkLuvGCICMReMcUT-AN8SzRg</recordid><startdate>19980301</startdate><enddate>19980301</enddate><creator>Mitsuda, T</creator><creator>Muto, T</creator><creator>Yamada, M</creator><creator>Kobayashi, N</creator><creator>Toba, M</creator><creator>Aihara, Y</creator><creator>Ito, A</creator><creator>Yokota, S</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19980301</creationdate><title>Epidemiological study of a food-borne outbreak of enterotoxigenic Escherichia coli O25:NM by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA analysis</title><author>Mitsuda, T ; Muto, T ; Yamada, M ; Kobayashi, N ; Toba, M ; Aihara, Y ; Ito, A ; Yokota, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-b1bd3688936d2b7a081bb3c256ad215bc3a1abd8d650b1bd6a45e094be6d28283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the digestive system and abdomen</topic><topic>Bacterial Toxins - analysis</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Disease Outbreaks</topic><topic>DNA, Bacterial - genetics</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Enterotoxins - analysis</topic><topic>Epidemiology</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli Infections - epidemiology</topic><topic>Escherichia coli Infections - microbiology</topic><topic>fish paste</topic><topic>Fish Products - microbiology</topic><topic>Food Microbiology</topic><topic>foodborne illness</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Molecular Epidemiology</topic><topic>Phenotype</topic><topic>Plasmids</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>Tuna - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mitsuda, T</creatorcontrib><creatorcontrib>Muto, T</creatorcontrib><creatorcontrib>Yamada, M</creatorcontrib><creatorcontrib>Kobayashi, N</creatorcontrib><creatorcontrib>Toba, M</creatorcontrib><creatorcontrib>Aihara, Y</creatorcontrib><creatorcontrib>Ito, A</creatorcontrib><creatorcontrib>Yokota, S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mitsuda, T</au><au>Muto, T</au><au>Yamada, M</au><au>Kobayashi, N</au><au>Toba, M</au><au>Aihara, Y</au><au>Ito, A</au><au>Yokota, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epidemiological study of a food-borne outbreak of enterotoxigenic Escherichia coli O25:NM by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA analysis</atitle><jtitle>Journal of clinical microbiology</jtitle><addtitle>J Clin Microbiol</addtitle><date>1998-03-01</date><risdate>1998</risdate><volume>36</volume><issue>3</issue><spage>652</spage><epage>656</epage><pages>652-656</pages><issn>0095-1137</issn><eissn>1098-660X</eissn><coden>JCMIDW</coden><abstract>This study investigated the applicability of molecular epidemiological techniques to the identification of the causal agent of an outbreak of diarrhea caused by ingestion of food contaminated with enterotoxigenic Escherichia coli (ETEC). The outbreak occurred at four elementary schools in July 1996 and affected more than 800 people. Illness was most strongly associated with eating tuna paste (relative risk, 1.79; 95% confidence interval = 1.16 to 2.79; P = 0.0001). To evaluate the epidemiological characteristics of the pathogen, the DNAs from numerous isolated ETEC strains were subjected to randomly amplified polymorphic DNA analysis, pulsed-field gel electrophoresis of nuclease S1-treated plasmid DNA, and analysis of genomic DNA restriction fragment length polymorphisms. All ETEC isolates were of the O25:NM (nonmotile) serotype, which carries a heat-stable enterotoxin Ib gene. Genotypic analysis demonstrated that the strains isolated from the patients at all four schools were identical. The isolates of ETEC O25:NM obtained from the tuna paste that had been served for lunch at these schools were genetically indistinguishable from those isolated from the patients. Results suggest that this outbreak was food borne. The molecular biology-based epidemiological techniques used in this study were useful in characterizing the causal agent in this food-borne epidemic.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>9508290</pmid><doi>10.1128/jcm.36.3.652-656.1998</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Bacterial diseases Bacterial diseases of the digestive system and abdomen Bacterial Toxins - analysis Bacteriology Biological and medical sciences Disease Outbreaks DNA, Bacterial - genetics Electrophoresis, Gel, Pulsed-Field Enterotoxins - analysis Epidemiology Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli Infections - epidemiology Escherichia coli Infections - microbiology fish paste Fish Products - microbiology Food Microbiology foodborne illness Fundamental and applied biological sciences. Psychology Genotype Human bacterial diseases Humans Infectious diseases Medical sciences Microbiology Molecular Epidemiology Phenotype Plasmids Polymorphism, Restriction Fragment Length Random Amplified Polymorphic DNA Technique Tuna - microbiology |
| title | Epidemiological study of a food-borne outbreak of enterotoxigenic Escherichia coli O25:NM by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA analysis |
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