Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses
Inotodiol, a lanostane-type triterpenoid, and many phytochemicals from Chaga mushrooms have been investigated for various allergic diseases. However, the anti-aging and anti-inflammatory activities of inotodiol under different types of oxidative stress and the impact of inotodiol on collagen and hya...
Gespeichert in:
| Veröffentlicht in: | International journal of molecular sciences 2023-08, Vol.24 (16), p.12803 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 16 |
| container_start_page | 12803 |
| container_title | International journal of molecular sciences |
| container_volume | 24 |
| creator | Park, Jihyun Nguyen, Thi Minh Nguyet Park, Hyun-ah Nguyen, My Tuyen Thi Lee, Nan-young Ban, So-young Park, Kyu-been Lee, Chang-kyu Kim, Jaehan Park, Jong-Tae |
| description | Inotodiol, a lanostane-type triterpenoid, and many phytochemicals from Chaga mushrooms have been investigated for various allergic diseases. However, the anti-aging and anti-inflammatory activities of inotodiol under different types of oxidative stress and the impact of inotodiol on collagen and hyaluronan synthesis have not been sufficiently studied. Lanostane triterpenoids-rich concentrate, which contained 10% inotodiol as major (inotodiol concentrate), was prepared from Chaga and compared with pure inotodiol in terms of anti-inflammatory activities on a human keratinocyte cell line, HaCaT cells, under various stimulations such as stimulation with ultraviolet (UV) B or tumor necrosis factor (TNF)-α. In stimulation with TNF-α, interleukin (IL)-1β, IL-6, and IL-8 genes were significantly repressed by 0.44~4.0 μg/mL of pure inotodiol. UVB irradiation induced the overexpression of pro-inflammatory cytokines, but those genes were significantly suppressed by pure inotodiol or inotodiol concentrate. Moreover, pure inotodiol/inotodiol concentrate could also modulate the synthesis of collagen and hyaluronic acid by controlling COL1A2 and HAS2/3 expression, which implies a crucial role for pure inotodiol/inotodiol concentrate in the prevention of skin aging. These results illuminate the anti-inflammatory and anti-aging effects of pure inotodiol/inotodiol concentrate, and it is highly conceivable that pure inotodiol and inotodiol concentrate could be promising natural bioactive substances to be incorporated in therapeutic and beautifying applications. |
| doi_str_mv | 10.3390/ijms241612803 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10454022</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A762477260</galeid><sourcerecordid>A762477260</sourcerecordid><originalsourceid>FETCH-LOGICAL-c460t-15048ed1b4b36a15819a28c805c0415194a072cc545a280c5e09b6e43db99f863</originalsourceid><addsrcrecordid>eNptUk1v1DAQjRCIlsKRuyUuHLrFn4lzQlVU2IpFRWI5W44z3vUqsRfbqdi_wS_G_RCwCPngmfF7b2Y8U1WvCb5grMXv3G5KlJOaUInZk-qUcEoXGNfN07_sk-pFSjuMKaOifV6dsKamsm3ZafXzSwwZTHa3gK6sLVZCwaKV9iFl7QGto8sQ9-CDGxKyMUyo2-qNRp_ntI2huM6j5Txpjz5B1Nn5YA4Z0jla6k6vUQfjWJzCcD5ldO3tqKdJ5xAPSPsB3fxwg75P_zVHSAnSy-qZ1WOCV4_3WfXtw9W6Wy5WNx-vu8vVwvAa5wURmEsYSM97VmsiJGk1lUZiYTAngrRc44YaI7gocWwE4LavgbOhb1sra3ZWvX_Q3c_9BIMBn6Me1T66SceDCtqp4xfvtmoTbhXBXHBMaVF4-6gQw_cZUlaTS6Y0XD4uzElRKRrJaZlNgb75B7oLc_Slv3sUlqVW8Qe10SMo520oic2dqLosI-NNQ2tcUBf_QZUzwORM8GBdiR8RFg8EE0NKEezvJglWd1ukjraI_QJMZLlS</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2857080725</pqid></control><display><type>article</type><title>Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Park, Jihyun ; Nguyen, Thi Minh Nguyet ; Park, Hyun-ah ; Nguyen, My Tuyen Thi ; Lee, Nan-young ; Ban, So-young ; Park, Kyu-been ; Lee, Chang-kyu ; Kim, Jaehan ; Park, Jong-Tae</creator><creatorcontrib>Park, Jihyun ; Nguyen, Thi Minh Nguyet ; Park, Hyun-ah ; Nguyen, My Tuyen Thi ; Lee, Nan-young ; Ban, So-young ; Park, Kyu-been ; Lee, Chang-kyu ; Kim, Jaehan ; Park, Jong-Tae</creatorcontrib><description>Inotodiol, a lanostane-type triterpenoid, and many phytochemicals from Chaga mushrooms have been investigated for various allergic diseases. However, the anti-aging and anti-inflammatory activities of inotodiol under different types of oxidative stress and the impact of inotodiol on collagen and hyaluronan synthesis have not been sufficiently studied. Lanostane triterpenoids-rich concentrate, which contained 10% inotodiol as major (inotodiol concentrate), was prepared from Chaga and compared with pure inotodiol in terms of anti-inflammatory activities on a human keratinocyte cell line, HaCaT cells, under various stimulations such as stimulation with ultraviolet (UV) B or tumor necrosis factor (TNF)-α. In stimulation with TNF-α, interleukin (IL)-1β, IL-6, and IL-8 genes were significantly repressed by 0.44~4.0 μg/mL of pure inotodiol. UVB irradiation induced the overexpression of pro-inflammatory cytokines, but those genes were significantly suppressed by pure inotodiol or inotodiol concentrate. Moreover, pure inotodiol/inotodiol concentrate could also modulate the synthesis of collagen and hyaluronic acid by controlling COL1A2 and HAS2/3 expression, which implies a crucial role for pure inotodiol/inotodiol concentrate in the prevention of skin aging. These results illuminate the anti-inflammatory and anti-aging effects of pure inotodiol/inotodiol concentrate, and it is highly conceivable that pure inotodiol and inotodiol concentrate could be promising natural bioactive substances to be incorporated in therapeutic and beautifying applications.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms241612803</identifier><identifier>PMID: 37628993</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Aging ; Antifungal agents ; B cells ; Collagen ; Comparative analysis ; Cytokines ; Cytotoxicity ; Fibroblasts ; Hyaluronic acid ; Inflammation ; Interleukins ; Kinases ; Medical research ; Medicine, Experimental ; Oxidative stress ; Senescence ; Skin ; Tumor necrosis factor ; Tumor necrosis factor-TNF ; Ultraviolet radiation</subject><ispartof>International journal of molecular sciences, 2023-08, Vol.24 (16), p.12803</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c460t-15048ed1b4b36a15819a28c805c0415194a072cc545a280c5e09b6e43db99f863</citedby><cites>FETCH-LOGICAL-c460t-15048ed1b4b36a15819a28c805c0415194a072cc545a280c5e09b6e43db99f863</cites><orcidid>0000-0001-9719-9957 ; 0000-0002-0650-6948</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454022/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10454022/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids></links><search><creatorcontrib>Park, Jihyun</creatorcontrib><creatorcontrib>Nguyen, Thi Minh Nguyet</creatorcontrib><creatorcontrib>Park, Hyun-ah</creatorcontrib><creatorcontrib>Nguyen, My Tuyen Thi</creatorcontrib><creatorcontrib>Lee, Nan-young</creatorcontrib><creatorcontrib>Ban, So-young</creatorcontrib><creatorcontrib>Park, Kyu-been</creatorcontrib><creatorcontrib>Lee, Chang-kyu</creatorcontrib><creatorcontrib>Kim, Jaehan</creatorcontrib><creatorcontrib>Park, Jong-Tae</creatorcontrib><title>Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses</title><title>International journal of molecular sciences</title><description>Inotodiol, a lanostane-type triterpenoid, and many phytochemicals from Chaga mushrooms have been investigated for various allergic diseases. However, the anti-aging and anti-inflammatory activities of inotodiol under different types of oxidative stress and the impact of inotodiol on collagen and hyaluronan synthesis have not been sufficiently studied. Lanostane triterpenoids-rich concentrate, which contained 10% inotodiol as major (inotodiol concentrate), was prepared from Chaga and compared with pure inotodiol in terms of anti-inflammatory activities on a human keratinocyte cell line, HaCaT cells, under various stimulations such as stimulation with ultraviolet (UV) B or tumor necrosis factor (TNF)-α. In stimulation with TNF-α, interleukin (IL)-1β, IL-6, and IL-8 genes were significantly repressed by 0.44~4.0 μg/mL of pure inotodiol. UVB irradiation induced the overexpression of pro-inflammatory cytokines, but those genes were significantly suppressed by pure inotodiol or inotodiol concentrate. Moreover, pure inotodiol/inotodiol concentrate could also modulate the synthesis of collagen and hyaluronic acid by controlling COL1A2 and HAS2/3 expression, which implies a crucial role for pure inotodiol/inotodiol concentrate in the prevention of skin aging. These results illuminate the anti-inflammatory and anti-aging effects of pure inotodiol/inotodiol concentrate, and it is highly conceivable that pure inotodiol and inotodiol concentrate could be promising natural bioactive substances to be incorporated in therapeutic and beautifying applications.</description><subject>Aging</subject><subject>Antifungal agents</subject><subject>B cells</subject><subject>Collagen</subject><subject>Comparative analysis</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Fibroblasts</subject><subject>Hyaluronic acid</subject><subject>Inflammation</subject><subject>Interleukins</subject><subject>Kinases</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Oxidative stress</subject><subject>Senescence</subject><subject>Skin</subject><subject>Tumor necrosis factor</subject><subject>Tumor necrosis factor-TNF</subject><subject>Ultraviolet radiation</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptUk1v1DAQjRCIlsKRuyUuHLrFn4lzQlVU2IpFRWI5W44z3vUqsRfbqdi_wS_G_RCwCPngmfF7b2Y8U1WvCb5grMXv3G5KlJOaUInZk-qUcEoXGNfN07_sk-pFSjuMKaOifV6dsKamsm3ZafXzSwwZTHa3gK6sLVZCwaKV9iFl7QGto8sQ9-CDGxKyMUyo2-qNRp_ntI2huM6j5Txpjz5B1Nn5YA4Z0jla6k6vUQfjWJzCcD5ldO3tqKdJ5xAPSPsB3fxwg75P_zVHSAnSy-qZ1WOCV4_3WfXtw9W6Wy5WNx-vu8vVwvAa5wURmEsYSM97VmsiJGk1lUZiYTAngrRc44YaI7gocWwE4LavgbOhb1sra3ZWvX_Q3c_9BIMBn6Me1T66SceDCtqp4xfvtmoTbhXBXHBMaVF4-6gQw_cZUlaTS6Y0XD4uzElRKRrJaZlNgb75B7oLc_Slv3sUlqVW8Qe10SMo520oic2dqLosI-NNQ2tcUBf_QZUzwORM8GBdiR8RFg8EE0NKEezvJglWd1ukjraI_QJMZLlS</recordid><startdate>20230815</startdate><enddate>20230815</enddate><creator>Park, Jihyun</creator><creator>Nguyen, Thi Minh Nguyet</creator><creator>Park, Hyun-ah</creator><creator>Nguyen, My Tuyen Thi</creator><creator>Lee, Nan-young</creator><creator>Ban, So-young</creator><creator>Park, Kyu-been</creator><creator>Lee, Chang-kyu</creator><creator>Kim, Jaehan</creator><creator>Park, Jong-Tae</creator><general>MDPI AG</general><general>MDPI</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-9719-9957</orcidid><orcidid>https://orcid.org/0000-0002-0650-6948</orcidid></search><sort><creationdate>20230815</creationdate><title>Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses</title><author>Park, Jihyun ; Nguyen, Thi Minh Nguyet ; Park, Hyun-ah ; Nguyen, My Tuyen Thi ; Lee, Nan-young ; Ban, So-young ; Park, Kyu-been ; Lee, Chang-kyu ; Kim, Jaehan ; Park, Jong-Tae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c460t-15048ed1b4b36a15819a28c805c0415194a072cc545a280c5e09b6e43db99f863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Aging</topic><topic>Antifungal agents</topic><topic>B cells</topic><topic>Collagen</topic><topic>Comparative analysis</topic><topic>Cytokines</topic><topic>Cytotoxicity</topic><topic>Fibroblasts</topic><topic>Hyaluronic acid</topic><topic>Inflammation</topic><topic>Interleukins</topic><topic>Kinases</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>Oxidative stress</topic><topic>Senescence</topic><topic>Skin</topic><topic>Tumor necrosis factor</topic><topic>Tumor necrosis factor-TNF</topic><topic>Ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Jihyun</creatorcontrib><creatorcontrib>Nguyen, Thi Minh Nguyet</creatorcontrib><creatorcontrib>Park, Hyun-ah</creatorcontrib><creatorcontrib>Nguyen, My Tuyen Thi</creatorcontrib><creatorcontrib>Lee, Nan-young</creatorcontrib><creatorcontrib>Ban, So-young</creatorcontrib><creatorcontrib>Park, Kyu-been</creatorcontrib><creatorcontrib>Lee, Chang-kyu</creatorcontrib><creatorcontrib>Kim, Jaehan</creatorcontrib><creatorcontrib>Park, Jong-Tae</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Jihyun</au><au>Nguyen, Thi Minh Nguyet</au><au>Park, Hyun-ah</au><au>Nguyen, My Tuyen Thi</au><au>Lee, Nan-young</au><au>Ban, So-young</au><au>Park, Kyu-been</au><au>Lee, Chang-kyu</au><au>Kim, Jaehan</au><au>Park, Jong-Tae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses</atitle><jtitle>International journal of molecular sciences</jtitle><date>2023-08-15</date><risdate>2023</risdate><volume>24</volume><issue>16</issue><spage>12803</spage><pages>12803-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Inotodiol, a lanostane-type triterpenoid, and many phytochemicals from Chaga mushrooms have been investigated for various allergic diseases. However, the anti-aging and anti-inflammatory activities of inotodiol under different types of oxidative stress and the impact of inotodiol on collagen and hyaluronan synthesis have not been sufficiently studied. Lanostane triterpenoids-rich concentrate, which contained 10% inotodiol as major (inotodiol concentrate), was prepared from Chaga and compared with pure inotodiol in terms of anti-inflammatory activities on a human keratinocyte cell line, HaCaT cells, under various stimulations such as stimulation with ultraviolet (UV) B or tumor necrosis factor (TNF)-α. In stimulation with TNF-α, interleukin (IL)-1β, IL-6, and IL-8 genes were significantly repressed by 0.44~4.0 μg/mL of pure inotodiol. UVB irradiation induced the overexpression of pro-inflammatory cytokines, but those genes were significantly suppressed by pure inotodiol or inotodiol concentrate. Moreover, pure inotodiol/inotodiol concentrate could also modulate the synthesis of collagen and hyaluronic acid by controlling COL1A2 and HAS2/3 expression, which implies a crucial role for pure inotodiol/inotodiol concentrate in the prevention of skin aging. These results illuminate the anti-inflammatory and anti-aging effects of pure inotodiol/inotodiol concentrate, and it is highly conceivable that pure inotodiol and inotodiol concentrate could be promising natural bioactive substances to be incorporated in therapeutic and beautifying applications.</abstract><cop>Basel</cop><pub>MDPI AG</pub><pmid>37628993</pmid><doi>10.3390/ijms241612803</doi><orcidid>https://orcid.org/0000-0001-9719-9957</orcidid><orcidid>https://orcid.org/0000-0002-0650-6948</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1422-0067 |
| ispartof | International journal of molecular sciences, 2023-08, Vol.24 (16), p.12803 |
| issn | 1422-0067 1661-6596 1422-0067 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10454022 |
| source | MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Aging Antifungal agents B cells Collagen Comparative analysis Cytokines Cytotoxicity Fibroblasts Hyaluronic acid Inflammation Interleukins Kinases Medical research Medicine, Experimental Oxidative stress Senescence Skin Tumor necrosis factor Tumor necrosis factor-TNF Ultraviolet radiation |
| title | Protective Effects of Lanostane Triterpenoids from Chaga Mushroom in Human Keratinocytes, HaCaT Cells, against Inflammatory and Oxidative Stresses |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T16%3A07%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protective%20Effects%20of%20Lanostane%20Triterpenoids%20from%20Chaga%20Mushroom%20in%20Human%20Keratinocytes,%20HaCaT%20Cells,%20against%20Inflammatory%20and%20Oxidative%20Stresses&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Park,%20Jihyun&rft.date=2023-08-15&rft.volume=24&rft.issue=16&rft.spage=12803&rft.pages=12803-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms241612803&rft_dat=%3Cgale_pubme%3EA762477260%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2857080725&rft_id=info:pmid/37628993&rft_galeid=A762477260&rfr_iscdi=true |