RNA sequencing identified novel target genes for Adansonia digitata in breast and colon cancer cells
Adansonia digitata exhibits numerous beneficial effects. In the current study, we investigated the anti-cancer effects of four different extracts of A. digitata (polar and non-polar extracts of fruit powder and fibers) on the proliferation of human colon cancer (HCT116), human breast cancer (MCF-7),...
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| Veröffentlicht in: | Science progress (1916) 2021-07, Vol.104 (3), p.368504211032084-368504211032084 |
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| creator | El-Masry, Omar S. Goja, Arafat Rateb, Mostafa Owaidah, Amani Y Alsamman, Khaldoon |
| description | Adansonia digitata exhibits numerous beneficial effects. In the current study, we investigated the anti-cancer effects of four different extracts of A. digitata (polar and non-polar extracts of fruit powder and fibers) on the proliferation of human colon cancer (HCT116), human breast cancer (MCF-7), and human ovarian cancer (OVCAR-3 and OVCAR-4) cell lines. RNA sequencing revealed the influence of the effective A. digitata fraction on the gene expression profiles of responsive cells. The results indicated that only the polar extract of the A. digitata fibers exhibited anti-proliferative activities against HCT116 and MCF-7 cells, but not ovarian cancer cells. Moreover, the polar extract of the fibers resulted in the modulation of the expression of multiple genes in HCT116 and MCF-7 cells. We propose that casein kinase 2 alpha 3 (CSNK2A3) is a novel casein kinase 2 (CSNK2) isoform in HCT116 cells and report, for the first time, the potential involvement of FYVE, RhoGEF, and PH domain-containing 3 (FGD3) in colon cancer. Together, these findings provide evidence supporting the anti-cancer potential of the polar extract of A. digitata fibers in this experimental model of breast and colon cancers. |
| doi_str_mv | 10.1177/00368504211032084 |
| format | Article |
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In the current study, we investigated the anti-cancer effects of four different extracts of A. digitata (polar and non-polar extracts of fruit powder and fibers) on the proliferation of human colon cancer (HCT116), human breast cancer (MCF-7), and human ovarian cancer (OVCAR-3 and OVCAR-4) cell lines. RNA sequencing revealed the influence of the effective A. digitata fraction on the gene expression profiles of responsive cells. The results indicated that only the polar extract of the A. digitata fibers exhibited anti-proliferative activities against HCT116 and MCF-7 cells, but not ovarian cancer cells. Moreover, the polar extract of the fibers resulted in the modulation of the expression of multiple genes in HCT116 and MCF-7 cells. We propose that casein kinase 2 alpha 3 (CSNK2A3) is a novel casein kinase 2 (CSNK2) isoform in HCT116 cells and report, for the first time, the potential involvement of FYVE, RhoGEF, and PH domain-containing 3 (FGD3) in colon cancer. Together, these findings provide evidence supporting the anti-cancer potential of the polar extract of A. digitata fibers in this experimental model of breast and colon cancers.</description><identifier>ISSN: 0036-8504</identifier><identifier>EISSN: 2047-7163</identifier><identifier>DOI: 10.1177/00368504211032084</identifier><identifier>PMID: 34251294</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Adansonia ; Adansonia digitata ; Anticancer properties ; Apoptosis ; Breast cancer ; Casein ; Casein Kinase II ; Cell Line, Tumor ; Colon ; Colon cancer ; Colonic Neoplasms - drug therapy ; Colonic Neoplasms - genetics ; Colorectal cancer ; Female ; Fibers ; Gene expression ; Gene sequencing ; Genes ; Humans ; Kinases ; Ovarian cancer ; Ovarian Neoplasms ; Plant Extracts ; Ribonucleic acid ; RNA ; Sequence Analysis, RNA ; Tumor cell lines</subject><ispartof>Science progress (1916), 2021-07, Vol.104 (3), p.368504211032084-368504211032084</ispartof><rights>The Author(s) 2021</rights><rights>2021. This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License ( https://creativecommons.org/licenses/by-nc/4.0/ ) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages ( https://us.sagepub.com/en-us/nam/open-access-at-sage ). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2021 2021 SAGE Publications</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-8f7d8ab67e69bcf0be954ae36ba22365c2d0b2b30e75d1e41aba70f03f72ec4c3</citedby><cites>FETCH-LOGICAL-c467t-8f7d8ab67e69bcf0be954ae36ba22365c2d0b2b30e75d1e41aba70f03f72ec4c3</cites><orcidid>0000-0002-0226-2923</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450698/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10450698/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,21966,27853,27924,27925,44945,45333,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34251294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El-Masry, Omar S.</creatorcontrib><creatorcontrib>Goja, Arafat</creatorcontrib><creatorcontrib>Rateb, Mostafa</creatorcontrib><creatorcontrib>Owaidah, Amani Y</creatorcontrib><creatorcontrib>Alsamman, Khaldoon</creatorcontrib><title>RNA sequencing identified novel target genes for Adansonia digitata in breast and colon cancer cells</title><title>Science progress (1916)</title><addtitle>Sci Prog</addtitle><description>Adansonia digitata exhibits numerous beneficial effects. In the current study, we investigated the anti-cancer effects of four different extracts of A. digitata (polar and non-polar extracts of fruit powder and fibers) on the proliferation of human colon cancer (HCT116), human breast cancer (MCF-7), and human ovarian cancer (OVCAR-3 and OVCAR-4) cell lines. RNA sequencing revealed the influence of the effective A. digitata fraction on the gene expression profiles of responsive cells. The results indicated that only the polar extract of the A. digitata fibers exhibited anti-proliferative activities against HCT116 and MCF-7 cells, but not ovarian cancer cells. Moreover, the polar extract of the fibers resulted in the modulation of the expression of multiple genes in HCT116 and MCF-7 cells. We propose that casein kinase 2 alpha 3 (CSNK2A3) is a novel casein kinase 2 (CSNK2) isoform in HCT116 cells and report, for the first time, the potential involvement of FYVE, RhoGEF, and PH domain-containing 3 (FGD3) in colon cancer. Together, these findings provide evidence supporting the anti-cancer potential of the polar extract of A. digitata fibers in this experimental model of breast and colon cancers.</description><subject>Adansonia</subject><subject>Adansonia digitata</subject><subject>Anticancer properties</subject><subject>Apoptosis</subject><subject>Breast cancer</subject><subject>Casein</subject><subject>Casein Kinase II</subject><subject>Cell Line, Tumor</subject><subject>Colon</subject><subject>Colon cancer</subject><subject>Colonic Neoplasms - drug therapy</subject><subject>Colonic Neoplasms - genetics</subject><subject>Colorectal cancer</subject><subject>Female</subject><subject>Fibers</subject><subject>Gene expression</subject><subject>Gene sequencing</subject><subject>Genes</subject><subject>Humans</subject><subject>Kinases</subject><subject>Ovarian cancer</subject><subject>Ovarian Neoplasms</subject><subject>Plant Extracts</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Sequence Analysis, RNA</subject><subject>Tumor cell lines</subject><issn>0036-8504</issn><issn>2047-7163</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>AFRWT</sourceid><sourceid>EIF</sourceid><recordid>eNp1kU2LFDEQhoMo7rj6A7xIwIuXXvPZ6T7JsLirsCiInkMlqW6z9CRr0rPgvzfDrOsXnupQT71Vb72EPOfsjHNjXjMm-0EzJThnUrBBPSAbwZTpDO_lQ7I59LsDcEKe1HrNGNe8Hx6TE6mE5mJUGxI-fdjSit_2mHxMM40B0xqniIGmfIsLXaHMuNIZE1Y65UK3AVLNKQINcY4rrEBjoq4g1JVCCtTnJSfqIXks1OOy1Kfk0QRLxWd39ZR8uXj7-fxdd_Xx8v359qrzqjdrN0wmDOB6g_3o_MQcjloByt6BELLXXgTmhJMMjQ4cFQcHhk1MTkagV16ekjdH3Zu922HwzUqBxd6UuIPy3WaI9s9Oil_tnG8tZ0qzfhyawqs7hZLbT-pqd7EePEDCvK9W6MYJLUbe0Jd_odd5X1Lz1ygzMKO5kY3iR8qXXGvB6f4azuwhRPtPiG3mxe827id-ptaAsyNQYcZfa_-v-AMUKKVh</recordid><startdate>20210701</startdate><enddate>20210701</enddate><creator>El-Masry, Omar S.</creator><creator>Goja, Arafat</creator><creator>Rateb, Mostafa</creator><creator>Owaidah, Amani Y</creator><creator>Alsamman, Khaldoon</creator><general>SAGE Publications</general><general>Sage Publications Ltd</general><scope>AFRWT</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>JQ2</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0226-2923</orcidid></search><sort><creationdate>20210701</creationdate><title>RNA sequencing identified novel target genes for Adansonia digitata in breast and colon cancer cells</title><author>El-Masry, Omar S. ; Goja, Arafat ; Rateb, Mostafa ; Owaidah, Amani Y ; Alsamman, Khaldoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-8f7d8ab67e69bcf0be954ae36ba22365c2d0b2b30e75d1e41aba70f03f72ec4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adansonia</topic><topic>Adansonia digitata</topic><topic>Anticancer properties</topic><topic>Apoptosis</topic><topic>Breast cancer</topic><topic>Casein</topic><topic>Casein Kinase II</topic><topic>Cell Line, Tumor</topic><topic>Colon</topic><topic>Colon cancer</topic><topic>Colonic Neoplasms - drug therapy</topic><topic>Colonic Neoplasms - genetics</topic><topic>Colorectal cancer</topic><topic>Female</topic><topic>Fibers</topic><topic>Gene expression</topic><topic>Gene sequencing</topic><topic>Genes</topic><topic>Humans</topic><topic>Kinases</topic><topic>Ovarian cancer</topic><topic>Ovarian Neoplasms</topic><topic>Plant Extracts</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Sequence Analysis, RNA</topic><topic>Tumor cell lines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El-Masry, Omar S.</creatorcontrib><creatorcontrib>Goja, Arafat</creatorcontrib><creatorcontrib>Rateb, Mostafa</creatorcontrib><creatorcontrib>Owaidah, Amani Y</creatorcontrib><creatorcontrib>Alsamman, Khaldoon</creatorcontrib><collection>Sage Journals GOLD Open Access 2024</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Science progress (1916)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El-Masry, Omar S.</au><au>Goja, Arafat</au><au>Rateb, Mostafa</au><au>Owaidah, Amani Y</au><au>Alsamman, Khaldoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNA sequencing identified novel target genes for Adansonia digitata in breast and colon cancer cells</atitle><jtitle>Science progress (1916)</jtitle><addtitle>Sci Prog</addtitle><date>2021-07-01</date><risdate>2021</risdate><volume>104</volume><issue>3</issue><spage>368504211032084</spage><epage>368504211032084</epage><pages>368504211032084-368504211032084</pages><issn>0036-8504</issn><eissn>2047-7163</eissn><abstract>Adansonia digitata exhibits numerous beneficial effects. In the current study, we investigated the anti-cancer effects of four different extracts of A. digitata (polar and non-polar extracts of fruit powder and fibers) on the proliferation of human colon cancer (HCT116), human breast cancer (MCF-7), and human ovarian cancer (OVCAR-3 and OVCAR-4) cell lines. RNA sequencing revealed the influence of the effective A. digitata fraction on the gene expression profiles of responsive cells. The results indicated that only the polar extract of the A. digitata fibers exhibited anti-proliferative activities against HCT116 and MCF-7 cells, but not ovarian cancer cells. Moreover, the polar extract of the fibers resulted in the modulation of the expression of multiple genes in HCT116 and MCF-7 cells. We propose that casein kinase 2 alpha 3 (CSNK2A3) is a novel casein kinase 2 (CSNK2) isoform in HCT116 cells and report, for the first time, the potential involvement of FYVE, RhoGEF, and PH domain-containing 3 (FGD3) in colon cancer. Together, these findings provide evidence supporting the anti-cancer potential of the polar extract of A. digitata fibers in this experimental model of breast and colon cancers.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>34251294</pmid><doi>10.1177/00368504211032084</doi><orcidid>https://orcid.org/0000-0002-0226-2923</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adansonia Adansonia digitata Anticancer properties Apoptosis Breast cancer Casein Casein Kinase II Cell Line, Tumor Colon Colon cancer Colonic Neoplasms - drug therapy Colonic Neoplasms - genetics Colorectal cancer Female Fibers Gene expression Gene sequencing Genes Humans Kinases Ovarian cancer Ovarian Neoplasms Plant Extracts Ribonucleic acid RNA Sequence Analysis, RNA Tumor cell lines |
| title | RNA sequencing identified novel target genes for Adansonia digitata in breast and colon cancer cells |
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