Comprehensive isolation of extracellular vesicles and nanoparticles

There is an increasing appreciation for the heterogeneous nature of extracellular vesicles (EVs). In addition, two nonvesicular extracellular nanoparticles (NVEPs), exomeres and supermeres, have been discovered recently that are enriched in many cargo previously ascribed to EVs. The EV field has lar...

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Veröffentlicht in:	Nature protocols 2023-05, Vol.18 (5), p.1462-1487
Hauptverfasser:	Zhang, Qin, Jeppesen, Dennis K., Higginbotham, James N., Franklin, Jeffrey L., Coffey, Robert J.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/2230 631/80 692/4017 Analytical Chemistry Biological Techniques Biomarkers Biomedical and Life Sciences Biomolecules Blood plasma Cargo Cell culture Computational Biology/Bioinformatics Concentration gradient Density Extracellular Vesicles Filtration Fractionation Fractions High resolution Humans Life Sciences Microarrays Nanoparticles Organic Chemistry Populations Protocol Reproducibility of Results Therapeutic targets Ultracentrifugation Vesicles
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