Epidemiology of gastrointestinal infections: lessons learned from syndromic testing, Region Zealand, Denmark
The aim of this study was to investigate the value of syndromic diagnostic testing for a better understanding of the epidemiology of gastrointestinal infections in Denmark. Here we evaluated the QIAstat-Dx® Gastrointestinal (GI) Panel 1 assay on 18,610 fecal samples requested for analysis for enteri...
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| Veröffentlicht in: | European journal of clinical microbiology & infectious diseases 2023-09, Vol.42 (9), p.1091-1101 |
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| creator | Johansen, Rikke Lykke Schouw, Christian Højte Madsen, Tina Vasehus Nielsen, Xiaohui Chen Engberg, Jørgen |
| description | The aim of this study was to investigate the value of syndromic diagnostic testing for a better understanding of the epidemiology of gastrointestinal infections in Denmark. Here we evaluated the QIAstat-Dx® Gastrointestinal (GI) Panel 1 assay on 18,610 fecal samples requested for analysis for enteric pathogens in Region Zealand, Denmark, in 1 year (October 1, 2021, to September 30, 2022). In total, 6905 (37%) samples were detected positive for one or more diarrhoeal bacteria, viruses, and protozoa. The most common bacterial, viral, and parasitic pathogens detected with the QIAstat-Dx® Gastrointestinal Panel 1 were EPEC (in patients ≥ 2 years of age) (
n
= 1420 (20.6%)), rotavirus (
n
= 948 (13.7%)), and
Cryptosporidium
spp. (
n
= 196 (2.84%)). We identified a large diversity in infections likely reflecting substantial differences in the epidemiology including origin of infections, mode of transmission, seasonality, age-dependent susceptibility to disease, severity, and travel history. All pathogens were detected as both single and coinfections. Viral infections peaked in March with a positive rate of 31.6%, and bacterial infections peaked in August with a positive rate of 35.3%. ETEC,
Shigella
/EIEC, EAEC, and
P. shigelloides
were most related to travel activity, and coinfections were frequent. The distribution of
C
t
values varied significantly between the pathogens, with the lowest
C
t
values (median 17–18) observed in astrovirus, adenovirus, and rotavirus. Our results highlight the value of providing extensive diagnostic testing on fecal samples for sufficient detection of relevant diarrhoeal pathogens for optimal clinical care. |
| doi_str_mv | 10.1007/s10096-023-04642-5 |
| format | Article |
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n
= 1420 (20.6%)), rotavirus (
n
= 948 (13.7%)), and
Cryptosporidium
spp. (
n
= 196 (2.84%)). We identified a large diversity in infections likely reflecting substantial differences in the epidemiology including origin of infections, mode of transmission, seasonality, age-dependent susceptibility to disease, severity, and travel history. All pathogens were detected as both single and coinfections. Viral infections peaked in March with a positive rate of 31.6%, and bacterial infections peaked in August with a positive rate of 35.3%. ETEC,
Shigella
/EIEC, EAEC, and
P. shigelloides
were most related to travel activity, and coinfections were frequent. The distribution of
C
t
values varied significantly between the pathogens, with the lowest
C
t
values (median 17–18) observed in astrovirus, adenovirus, and rotavirus. Our results highlight the value of providing extensive diagnostic testing on fecal samples for sufficient detection of relevant diarrhoeal pathogens for optimal clinical care.</description><identifier>ISSN: 0934-9723</identifier><identifier>EISSN: 1435-4373</identifier><identifier>DOI: 10.1007/s10096-023-04642-5</identifier><identifier>PMID: 37468662</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Bacteria ; Bacterial diseases ; Bacterial infections ; Biomedical and Life Sciences ; Biomedicine ; Cryptosporidium ; Diagnostic systems ; Diagnostic tests ; Disease transmission ; Epidemiology ; Feces ; Infections ; Internal Medicine ; Medical Microbiology ; Original ; Original Article ; Parasitic diseases ; Pathogens ; Protozoa ; Rotavirus ; Seasonal variations ; Travel ; Viral infections ; Viruses</subject><ispartof>European journal of clinical microbiology & infectious diseases, 2023-09, Vol.42 (9), p.1091-1101</ispartof><rights>The Author(s) 2023</rights><rights>2023. The Author(s).</rights><rights>The Author(s) 2023. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-e967ec11d40b53a3cb6c29486daea320acb8b975634450de77d716633e00dc923</citedby><cites>FETCH-LOGICAL-c475t-e967ec11d40b53a3cb6c29486daea320acb8b975634450de77d716633e00dc923</cites><orcidid>0000-0002-0659-1513 ; 0000-0001-9042-1631 ; 0000-0003-0966-1123 ; 0000-0003-4068-5748 ; 0000-0002-6176-7112</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10096-023-04642-5$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10096-023-04642-5$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37468662$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johansen, Rikke Lykke</creatorcontrib><creatorcontrib>Schouw, Christian Højte</creatorcontrib><creatorcontrib>Madsen, Tina Vasehus</creatorcontrib><creatorcontrib>Nielsen, Xiaohui Chen</creatorcontrib><creatorcontrib>Engberg, Jørgen</creatorcontrib><title>Epidemiology of gastrointestinal infections: lessons learned from syndromic testing, Region Zealand, Denmark</title><title>European journal of clinical microbiology & infectious diseases</title><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><addtitle>Eur J Clin Microbiol Infect Dis</addtitle><description>The aim of this study was to investigate the value of syndromic diagnostic testing for a better understanding of the epidemiology of gastrointestinal infections in Denmark. Here we evaluated the QIAstat-Dx® Gastrointestinal (GI) Panel 1 assay on 18,610 fecal samples requested for analysis for enteric pathogens in Region Zealand, Denmark, in 1 year (October 1, 2021, to September 30, 2022). In total, 6905 (37%) samples were detected positive for one or more diarrhoeal bacteria, viruses, and protozoa. The most common bacterial, viral, and parasitic pathogens detected with the QIAstat-Dx® Gastrointestinal Panel 1 were EPEC (in patients ≥ 2 years of age) (
n
= 1420 (20.6%)), rotavirus (
n
= 948 (13.7%)), and
Cryptosporidium
spp. (
n
= 196 (2.84%)). We identified a large diversity in infections likely reflecting substantial differences in the epidemiology including origin of infections, mode of transmission, seasonality, age-dependent susceptibility to disease, severity, and travel history. All pathogens were detected as both single and coinfections. Viral infections peaked in March with a positive rate of 31.6%, and bacterial infections peaked in August with a positive rate of 35.3%. ETEC,
Shigella
/EIEC, EAEC, and
P. shigelloides
were most related to travel activity, and coinfections were frequent. The distribution of
C
t
values varied significantly between the pathogens, with the lowest
C
t
values (median 17–18) observed in astrovirus, adenovirus, and rotavirus. Our results highlight the value of providing extensive diagnostic testing on fecal samples for sufficient detection of relevant diarrhoeal pathogens for optimal clinical care.</description><subject>Bacteria</subject><subject>Bacterial diseases</subject><subject>Bacterial infections</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cryptosporidium</subject><subject>Diagnostic systems</subject><subject>Diagnostic tests</subject><subject>Disease transmission</subject><subject>Epidemiology</subject><subject>Feces</subject><subject>Infections</subject><subject>Internal Medicine</subject><subject>Medical Microbiology</subject><subject>Original</subject><subject>Original Article</subject><subject>Parasitic diseases</subject><subject>Pathogens</subject><subject>Protozoa</subject><subject>Rotavirus</subject><subject>Seasonal variations</subject><subject>Travel</subject><subject>Viral infections</subject><subject>Viruses</subject><issn>0934-9723</issn><issn>1435-4373</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1vFSEUhonR2NvqH3BhSNx00bEMMDDjxjRt_UiamBjduCEMnBmpM3CFuSb333vq1Pqx6AIOCc_7cg4vIc9q9rJmTJ8W3DtVMS4qJpXkVfOAbGopmkoKLR6SDeuErDrNxQE5LOWaoajV-jE5EFqqVim-IdPlNniYQ5rSuKdpoKMtS04hLlCWEO1EQxzALSHF8opOUAoesNocwdMhp5mWffRYg6OrZjyhH2FEAf0CdrLRn9ALiLPN356QR4OdCjy9rUfk85vLT-fvqqsPb9-fn11VTupmqaBTGlxde8n6RljheuV4J1vlLVjBmXV923e6UULKhnnQ2utaKSGAMe86Lo7I69V3u-tn8A7iku1ktjlgF3uTbDD_3sTw1Yzph6mZ5LqREh2Obx1y-r7DscwcioMJx4G0K4a3knGpcCH64j_0Ou0y_twN1bCultg6UnylXE6lZBjuuqmZuUnTrGkaTNP8StM0KHr-9xx3kt_xISBWoOBVHCH_efse2591u6yj</recordid><startdate>20230901</startdate><enddate>20230901</enddate><creator>Johansen, Rikke Lykke</creator><creator>Schouw, Christian Højte</creator><creator>Madsen, Tina Vasehus</creator><creator>Nielsen, Xiaohui Chen</creator><creator>Engberg, Jørgen</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-0659-1513</orcidid><orcidid>https://orcid.org/0000-0001-9042-1631</orcidid><orcidid>https://orcid.org/0000-0003-0966-1123</orcidid><orcidid>https://orcid.org/0000-0003-4068-5748</orcidid><orcidid>https://orcid.org/0000-0002-6176-7112</orcidid></search><sort><creationdate>20230901</creationdate><title>Epidemiology of gastrointestinal infections: lessons learned from syndromic testing, Region Zealand, Denmark</title><author>Johansen, Rikke Lykke ; 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Here we evaluated the QIAstat-Dx® Gastrointestinal (GI) Panel 1 assay on 18,610 fecal samples requested for analysis for enteric pathogens in Region Zealand, Denmark, in 1 year (October 1, 2021, to September 30, 2022). In total, 6905 (37%) samples were detected positive for one or more diarrhoeal bacteria, viruses, and protozoa. The most common bacterial, viral, and parasitic pathogens detected with the QIAstat-Dx® Gastrointestinal Panel 1 were EPEC (in patients ≥ 2 years of age) (
n
= 1420 (20.6%)), rotavirus (
n
= 948 (13.7%)), and
Cryptosporidium
spp. (
n
= 196 (2.84%)). We identified a large diversity in infections likely reflecting substantial differences in the epidemiology including origin of infections, mode of transmission, seasonality, age-dependent susceptibility to disease, severity, and travel history. All pathogens were detected as both single and coinfections. Viral infections peaked in March with a positive rate of 31.6%, and bacterial infections peaked in August with a positive rate of 35.3%. ETEC,
Shigella
/EIEC, EAEC, and
P. shigelloides
were most related to travel activity, and coinfections were frequent. The distribution of
C
t
values varied significantly between the pathogens, with the lowest
C
t
values (median 17–18) observed in astrovirus, adenovirus, and rotavirus. Our results highlight the value of providing extensive diagnostic testing on fecal samples for sufficient detection of relevant diarrhoeal pathogens for optimal clinical care.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>37468662</pmid><doi>10.1007/s10096-023-04642-5</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-0659-1513</orcidid><orcidid>https://orcid.org/0000-0001-9042-1631</orcidid><orcidid>https://orcid.org/0000-0003-0966-1123</orcidid><orcidid>https://orcid.org/0000-0003-4068-5748</orcidid><orcidid>https://orcid.org/0000-0002-6176-7112</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | European journal of clinical microbiology & infectious diseases, 2023-09, Vol.42 (9), p.1091-1101 |
| issn | 0934-9723 1435-4373 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10427544 |
| source | SpringerNature Journals |
| subjects | Bacteria Bacterial diseases Bacterial infections Biomedical and Life Sciences Biomedicine Cryptosporidium Diagnostic systems Diagnostic tests Disease transmission Epidemiology Feces Infections Internal Medicine Medical Microbiology Original Original Article Parasitic diseases Pathogens Protozoa Rotavirus Seasonal variations Travel Viral infections Viruses |
| title | Epidemiology of gastrointestinal infections: lessons learned from syndromic testing, Region Zealand, Denmark |
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