HTS driven by fluorescence lifetime detection of FRET identifies activators and inhibitors of cardiac myosin

Small molecules that bind to allosteric sites on target proteins to alter protein function are highly sought in drug discovery. High-throughput screening (HTS) assays are needed to facilitate the direct discovery of allosterically active compounds. We have developed technology for high-throughput ti...

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Veröffentlicht in:	SLAS discovery 2023-07, Vol.28 (5), p.223-232
Hauptverfasser:	Muretta, JM, Rajasekaran, D, Blat, Y, Little, S, Myers, M, Nair, C, Burdekin, B, Yuen, SL, Jimenez, N, Guhathakurta, P, Wilson, A, Thompson, AR, Surti, N, Connors, D, Chase, P, Harden, D, Barbieri, CM, Adam, L, Thomas, DD
Format:	Artikel
Sprache:	eng
Schlagworte:	allosteric Cardiac Myosins Drug Discovery - methods Fluorescence Resonance Energy Transfer - methods heart failure High-throughput screening High-Throughput Screening Assays - methods myosin time-resolved fret
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