Dynamic Detection of Specific Membrane Capacitance and Cytoplasmic Resistance of Neutrophils After Ischemic Stroke
Peripheral blood is the most readily available resource for stroke patient prognosis, but there is a lack of methods to detect dynamic changes of neutrophils in peripheral blood that can be used in the clinic. Herein, we developed a procedure to characterize dynamic changes of neutrophils based on t...
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| Veröffentlicht in: | Aging and disease 2023-08, Vol.14 (4), p.1035-1037 |
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| creator | Zhao, Haiping Luan, Xiaofeng Wang, Yuqing Ye, Yifei Yan, Feng Li, Xue Li, Yuang Li, Mingxiao Zhang, Lingqian Zhao, Yang Huang, Chengjun Luo, Yumin |
| description | Peripheral blood is the most readily available resource for stroke patient prognosis, but there is a lack of methods to detect dynamic changes of neutrophils in peripheral blood that can be used in the clinic. Herein, we developed a procedure to characterize dynamic changes of neutrophils based on their electrical properties in rats after transient middle cerebral artery occlusion (MCAO). We characterized the specific membrane capacitance (C
) and cytoplasmic resistance (σ
) of approximately 27,600 neutrophils from MCAO rats 24 h after ischemia/reperfusion. We found that the C
and σ
of neutrophils in the MCAO group were significantly higher compared to the sham group. Furthermore, we observed a monotonically upward shift in neutrophil Csm in the MCAO group during the four 5-minute test cycles. Our findings suggest that the dynamic changes of cellular electrical properties could reflect neutrophil activity and serve as a prognostic indicator for ischemic stroke in the clinical setting. |
| doi_str_mv | 10.14336/AD.2023.0127 |
| format | Article |
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) and cytoplasmic resistance (σ
) of approximately 27,600 neutrophils from MCAO rats 24 h after ischemia/reperfusion. We found that the C
and σ
of neutrophils in the MCAO group were significantly higher compared to the sham group. Furthermore, we observed a monotonically upward shift in neutrophil Csm in the MCAO group during the four 5-minute test cycles. Our findings suggest that the dynamic changes of cellular electrical properties could reflect neutrophil activity and serve as a prognostic indicator for ischemic stroke in the clinical setting.</description><identifier>ISSN: 2152-5250</identifier><identifier>EISSN: 2152-5250</identifier><identifier>DOI: 10.14336/AD.2023.0127</identifier><identifier>PMID: 37163431</identifier><language>eng</language><publisher>United States: JKL International</publisher><subject>Comparative analysis ; Development and progression ; Electric properties ; Electrophysiology ; Health aspects ; Ischemia ; Medical research ; Medicine, Experimental ; Microfluidics ; Neutrophils ; Physiological aspects ; Prognosis ; Stroke (Disease)</subject><ispartof>Aging and disease, 2023-08, Vol.14 (4), p.1035-1037</ispartof><rights>COPYRIGHT 2023 JKL International</rights><rights>Copyright: © 2023 Zhao et al. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c416t-87cafeb1113e2941d9e1833af7ccb2dbdc2cd19b270049f166d94d2875541eae3</citedby><cites>FETCH-LOGICAL-c416t-87cafeb1113e2941d9e1833af7ccb2dbdc2cd19b270049f166d94d2875541eae3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10389826/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10389826/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37163431$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Haiping</creatorcontrib><creatorcontrib>Luan, Xiaofeng</creatorcontrib><creatorcontrib>Wang, Yuqing</creatorcontrib><creatorcontrib>Ye, Yifei</creatorcontrib><creatorcontrib>Yan, Feng</creatorcontrib><creatorcontrib>Li, Xue</creatorcontrib><creatorcontrib>Li, Yuang</creatorcontrib><creatorcontrib>Li, Mingxiao</creatorcontrib><creatorcontrib>Zhang, Lingqian</creatorcontrib><creatorcontrib>Zhao, Yang</creatorcontrib><creatorcontrib>Huang, Chengjun</creatorcontrib><creatorcontrib>Luo, Yumin</creatorcontrib><title>Dynamic Detection of Specific Membrane Capacitance and Cytoplasmic Resistance of Neutrophils After Ischemic Stroke</title><title>Aging and disease</title><addtitle>Aging Dis</addtitle><description>Peripheral blood is the most readily available resource for stroke patient prognosis, but there is a lack of methods to detect dynamic changes of neutrophils in peripheral blood that can be used in the clinic. Herein, we developed a procedure to characterize dynamic changes of neutrophils based on their electrical properties in rats after transient middle cerebral artery occlusion (MCAO). We characterized the specific membrane capacitance (C
) and cytoplasmic resistance (σ
) of approximately 27,600 neutrophils from MCAO rats 24 h after ischemia/reperfusion. We found that the C
and σ
of neutrophils in the MCAO group were significantly higher compared to the sham group. Furthermore, we observed a monotonically upward shift in neutrophil Csm in the MCAO group during the four 5-minute test cycles. Our findings suggest that the dynamic changes of cellular electrical properties could reflect neutrophil activity and serve as a prognostic indicator for ischemic stroke in the clinical setting.</description><subject>Comparative analysis</subject><subject>Development and progression</subject><subject>Electric properties</subject><subject>Electrophysiology</subject><subject>Health aspects</subject><subject>Ischemia</subject><subject>Medical research</subject><subject>Medicine, Experimental</subject><subject>Microfluidics</subject><subject>Neutrophils</subject><subject>Physiological aspects</subject><subject>Prognosis</subject><subject>Stroke (Disease)</subject><issn>2152-5250</issn><issn>2152-5250</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNptkt2L1DAUxYso7rLuo69SEMSXjrlJ-vUkZcbVhVXB1eeQpjfbaNvUJBXmv9_UWZcZMHlIuPd3D-TkJMlLIBvgjBXvmt2GEso2BGj5JDmnkNMspzl5enQ_Sy69_0niYjVlNXuenLESCsYZnCdut5_kaFS6w4AqGDulVqe3MyqjY_Uzjq2TE6ZbOUtlgpwUpnLq0u0-2HmQfh39ht74QyvOfsElODv3ZvBpowO69NqrHlfwNjZ-4YvkmZaDx8uH8yL5cfXh-_ZTdvP14_W2uckUhyJkVamkxhYAGNKaQ1cjVIxJXSrV0q7tFFUd1C0tCeG1hqLoat7RqsxzDiiRXSTvD7rz0o7YKZyCk4OYnRml2wsrjTjtTKYXd_aPAMKquqJFVHj7oODs7wV9EKPxCochOmIXL2gF0V9eFSv6-oDeyQGFmbSNkmrFRVMWAJxAxSO1-Q8Vd7f6YyfUJtZPBt4cDfQoh9B7OyzrR_lTMDuAylnvHerHdwIRf7Mimp1YsyLWrET-1bE5j_S_ZLB75j242g</recordid><startdate>20230801</startdate><enddate>20230801</enddate><creator>Zhao, Haiping</creator><creator>Luan, Xiaofeng</creator><creator>Wang, Yuqing</creator><creator>Ye, Yifei</creator><creator>Yan, Feng</creator><creator>Li, Xue</creator><creator>Li, Yuang</creator><creator>Li, Mingxiao</creator><creator>Zhang, Lingqian</creator><creator>Zhao, Yang</creator><creator>Huang, Chengjun</creator><creator>Luo, Yumin</creator><general>JKL International</general><general>JKL International LLC</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20230801</creationdate><title>Dynamic Detection of Specific Membrane Capacitance and Cytoplasmic Resistance of Neutrophils After Ischemic Stroke</title><author>Zhao, Haiping ; Luan, Xiaofeng ; Wang, Yuqing ; Ye, Yifei ; Yan, Feng ; Li, Xue ; Li, Yuang ; Li, Mingxiao ; Zhang, Lingqian ; Zhao, Yang ; Huang, Chengjun ; Luo, Yumin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-87cafeb1113e2941d9e1833af7ccb2dbdc2cd19b270049f166d94d2875541eae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Comparative analysis</topic><topic>Development and progression</topic><topic>Electric properties</topic><topic>Electrophysiology</topic><topic>Health aspects</topic><topic>Ischemia</topic><topic>Medical research</topic><topic>Medicine, Experimental</topic><topic>Microfluidics</topic><topic>Neutrophils</topic><topic>Physiological aspects</topic><topic>Prognosis</topic><topic>Stroke (Disease)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Haiping</creatorcontrib><creatorcontrib>Luan, Xiaofeng</creatorcontrib><creatorcontrib>Wang, Yuqing</creatorcontrib><creatorcontrib>Ye, Yifei</creatorcontrib><creatorcontrib>Yan, Feng</creatorcontrib><creatorcontrib>Li, Xue</creatorcontrib><creatorcontrib>Li, Yuang</creatorcontrib><creatorcontrib>Li, Mingxiao</creatorcontrib><creatorcontrib>Zhang, Lingqian</creatorcontrib><creatorcontrib>Zhao, Yang</creatorcontrib><creatorcontrib>Huang, Chengjun</creatorcontrib><creatorcontrib>Luo, Yumin</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Aging and disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Haiping</au><au>Luan, Xiaofeng</au><au>Wang, Yuqing</au><au>Ye, Yifei</au><au>Yan, Feng</au><au>Li, Xue</au><au>Li, Yuang</au><au>Li, Mingxiao</au><au>Zhang, Lingqian</au><au>Zhao, Yang</au><au>Huang, Chengjun</au><au>Luo, Yumin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamic Detection of Specific Membrane Capacitance and Cytoplasmic Resistance of Neutrophils After Ischemic Stroke</atitle><jtitle>Aging and disease</jtitle><addtitle>Aging Dis</addtitle><date>2023-08-01</date><risdate>2023</risdate><volume>14</volume><issue>4</issue><spage>1035</spage><epage>1037</epage><pages>1035-1037</pages><issn>2152-5250</issn><eissn>2152-5250</eissn><abstract>Peripheral blood is the most readily available resource for stroke patient prognosis, but there is a lack of methods to detect dynamic changes of neutrophils in peripheral blood that can be used in the clinic. Herein, we developed a procedure to characterize dynamic changes of neutrophils based on their electrical properties in rats after transient middle cerebral artery occlusion (MCAO). We characterized the specific membrane capacitance (C
) and cytoplasmic resistance (σ
) of approximately 27,600 neutrophils from MCAO rats 24 h after ischemia/reperfusion. We found that the C
and σ
of neutrophils in the MCAO group were significantly higher compared to the sham group. Furthermore, we observed a monotonically upward shift in neutrophil Csm in the MCAO group during the four 5-minute test cycles. Our findings suggest that the dynamic changes of cellular electrical properties could reflect neutrophil activity and serve as a prognostic indicator for ischemic stroke in the clinical setting.</abstract><cop>United States</cop><pub>JKL International</pub><pmid>37163431</pmid><doi>10.14336/AD.2023.0127</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Comparative analysis Development and progression Electric properties Electrophysiology Health aspects Ischemia Medical research Medicine, Experimental Microfluidics Neutrophils Physiological aspects Prognosis Stroke (Disease) |
| title | Dynamic Detection of Specific Membrane Capacitance and Cytoplasmic Resistance of Neutrophils After Ischemic Stroke |
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