Tissue Washing Improves Native Ambient Mass Spectrometry Detection of Membrane Proteins Directly from Tissue

Tissue Washing Improves Native Ambient Mass Spectrometry Detection of Membrane Proteins Directly from Tissue

Native ambient mass spectrometry enables the in situ analysis of proteins and their complexes directly from tissue, providing both structural and spatial information. Until recently, the approach was applied exclusively to the analysis of soluble proteins; however, there is a drive for new technique...

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Veröffentlicht in:Journal of the American Chemical Society 2023-07, Vol.145 (29), p.15658-15662
Hauptverfasser: Sisley, Emma K., Hale, Oliver J., Hughes, James W., Cooper, Helen J.
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Communication
Mass Spectrometry - methods
Membrane Proteins - chemistry
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container_end_page 15662
container_issue 29
container_start_page 15658
container_title Journal of the American Chemical Society
container_volume 145
creator Sisley, Emma K.
Hale, Oliver J.
Hughes, James W.
Cooper, Helen J.
description Native ambient mass spectrometry enables the in situ analysis of proteins and their complexes directly from tissue, providing both structural and spatial information. Until recently, the approach was applied exclusively to the analysis of soluble proteins; however, there is a drive for new techniques that enable analysis of membrane proteins. Here we demonstrate native ambient mass spectrometry of membrane proteins, including β-barrel and α-helical (single and multipass) integral membrane proteins and membrane-associated proteins incorporating lipid anchors, by integration of a simple washing protocol to remove soluble proteins. Mass spectrometry imaging revealed that washing did not disrupt the spatial distributions of the membrane and membrane-associated proteins. Some delocalization of the remaining soluble proteins was observed.
doi_str_mv 10.1021/jacs.3c03454
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Mass Spectrometry - methods
Membrane Proteins - chemistry
title Tissue Washing Improves Native Ambient Mass Spectrometry Detection of Membrane Proteins Directly from Tissue
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