Rapid detection of SARS-CoV-2 RNA in saliva via Cas13

Rapid nucleic acid testing is central to infectious disease surveillance. Here, we report an assay for rapid COVID-19 testing and its implementation in a prototype microfluidic device. The assay, which we named DISCoVER (for diagnostics with coronavirus enzymatic reporting), involves extraction-free...

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Veröffentlicht in:	Nature biomedical engineering 2022-08, Vol.6 (8), p.944-956
Hauptverfasser:	Chandrasekaran, Sita S., Agrawal, Shreeya, Fanton, Alison, Jangid, Aditya R., Charrez, Bérénice, Escajeda, Arturo M., Son, Sungmin, Mcintosh, Roger, Tran, Huyen, Bhuiya, Abdul, de León Derby, María Díaz, Switz, Neil A., Armstrong, Maxim, Harris, Andrew R., Prywes, Noam, Lukarska, Maria, Biering, Scott B., Smock, Dylan C. J., Mok, Amanda, Knott, Gavin J., Dang, Qi, Van Dis, Erik, Dugan, Eli, Kim, Shin, Liu, Tina Y., Moehle, Erica A., Kogut, Katherine, Eskenazi, Brenda, Harris, Eva, Stanley, Sarah A., Lareau, Liana F., Tan, Ming X., Fletcher, Daniel A., Doudna, Jennifer A., Savage, David F., Hsu, Patrick D.
Format:	Artikel
Sprache:	eng
Schlagworte:	631/45/607 692/700/139 82/29 82/62 82/80 82/83 Amplification Assaying Automation Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Cleavage Coronaviruses COVID-19 COVID-19 - diagnosis COVID-19 diagnostic tests COVID-19 Testing Humans Infectious diseases Lysis Microfluidic devices Microfluidics Nucleic acids Quenching Reagents Ribonucleic acid RNA RNA, Viral - genetics Saliva SARS-CoV-2 - genetics Severe acute respiratory syndrome coronavirus 2 Viral diseases
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