Dual-ratio approach for detection of point fluorophores in biological tissue
Diffuse flow cytometry (DiFC) is an emerging fluorescence sensing method to non-invasively detect labeled circulating cells . However, due to signal-to-noise ratio (SNR) constraints largely attributed to background tissue autofluorescence (AF), DiFC's measurement depth is limited. The dual rati...
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| Veröffentlicht in: | Journal of biomedical optics 2023-07, Vol.28 (7), p.077001 |
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| creator | Blaney, Giles Ivich, Fernando Sassaroli, Angelo Niedre, Mark Fantini, Sergio |
| description | Diffuse
flow cytometry (DiFC) is an emerging fluorescence sensing method to non-invasively detect labeled circulating cells
. However, due to signal-to-noise ratio (SNR) constraints largely attributed to background tissue autofluorescence (AF), DiFC's measurement depth is limited.
The dual ratio (DR)/dual slope is an optical measurement method that aims to suppress noise and enhance SNR to deep tissue regions. We aim to investigate the combination of DR and near-infrared (NIR) DiFC to improve circulating cells' maximum detectable depth and SNR.
Phantom experiments were used to estimate the key parameters in a diffuse fluorescence excitation and emission model. This model and parameters were implemented in Monte Carlo to simulate DR DiFC while varying noise and AF parameters to identify the advantages and limitations of the proposed technique.
Two key factors must be true to give DR DiFC an advantage over traditional DiFC: first, the fraction of noise that DR methods cannot cancel cannot be above the order of 10% for acceptable SNR. Second, DR DiFC has an advantage, in terms of SNR, if the distribution of tissue AF contributors is surface-weighted.
DR cancelable noise may be designed (e.g., through the use of source multiplexing), and indications point to the AF contributors' distribution being truly surface-weighted
. Successful and worthwhile implementation of DR DiFC depends on these considerations, but results point to DR DiFC having possible advantages over traditional DiFC. |
| doi_str_mv | 10.1117/1.JBO.28.7.077001 |
| format | Article |
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flow cytometry (DiFC) is an emerging fluorescence sensing method to non-invasively detect labeled circulating cells
. However, due to signal-to-noise ratio (SNR) constraints largely attributed to background tissue autofluorescence (AF), DiFC's measurement depth is limited.
The dual ratio (DR)/dual slope is an optical measurement method that aims to suppress noise and enhance SNR to deep tissue regions. We aim to investigate the combination of DR and near-infrared (NIR) DiFC to improve circulating cells' maximum detectable depth and SNR.
Phantom experiments were used to estimate the key parameters in a diffuse fluorescence excitation and emission model. This model and parameters were implemented in Monte Carlo to simulate DR DiFC while varying noise and AF parameters to identify the advantages and limitations of the proposed technique.
Two key factors must be true to give DR DiFC an advantage over traditional DiFC: first, the fraction of noise that DR methods cannot cancel cannot be above the order of 10% for acceptable SNR. Second, DR DiFC has an advantage, in terms of SNR, if the distribution of tissue AF contributors is surface-weighted.
DR cancelable noise may be designed (e.g., through the use of source multiplexing), and indications point to the AF contributors' distribution being truly surface-weighted
. Successful and worthwhile implementation of DR DiFC depends on these considerations, but results point to DR DiFC having possible advantages over traditional DiFC.</description><identifier>ISSN: 1083-3668</identifier><identifier>ISSN: 1560-2281</identifier><identifier>EISSN: 1560-2281</identifier><identifier>DOI: 10.1117/1.JBO.28.7.077001</identifier><identifier>PMID: 37484977</identifier><language>eng</language><publisher>United States: S P I E - International Society for</publisher><subject>Background noise ; Blood ; Blood vessels ; Cancer ; Chemical compounds ; Flow cytometry ; Fluorescence ; Fluorophores ; In vivo methods and tests ; Mathematical models ; Measurement methods ; Medical prognosis ; Medical research ; Multiplexing ; Optical measurement ; Parameter identification ; Phantoms, Imaging ; Sensing ; Sensors ; Signal to noise ratio ; Tissues ; Veins & arteries</subject><ispartof>Journal of biomedical optics, 2023-07, Vol.28 (7), p.077001</ispartof><rights>2023 The Authors.</rights><rights>2023. This work is licensed under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 The Authors 2023 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-900a2e16355cadd1483588e553c49d882933f393f36663e118f2ad60479c54dd3</citedby><cites>FETCH-LOGICAL-c428t-900a2e16355cadd1483588e553c49d882933f393f36663e118f2ad60479c54dd3</cites><orcidid>0000-0001-5116-525X ; 0000-0003-2830-3161 ; 0000-0003-3419-4547</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2858378846/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2858378846?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,21367,27901,27902,33721,33722,43781,53766,53768,74045</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37484977$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blaney, Giles</creatorcontrib><creatorcontrib>Ivich, Fernando</creatorcontrib><creatorcontrib>Sassaroli, Angelo</creatorcontrib><creatorcontrib>Niedre, Mark</creatorcontrib><creatorcontrib>Fantini, Sergio</creatorcontrib><title>Dual-ratio approach for detection of point fluorophores in biological tissue</title><title>Journal of biomedical optics</title><addtitle>J Biomed Opt</addtitle><description>Diffuse
flow cytometry (DiFC) is an emerging fluorescence sensing method to non-invasively detect labeled circulating cells
. However, due to signal-to-noise ratio (SNR) constraints largely attributed to background tissue autofluorescence (AF), DiFC's measurement depth is limited.
The dual ratio (DR)/dual slope is an optical measurement method that aims to suppress noise and enhance SNR to deep tissue regions. We aim to investigate the combination of DR and near-infrared (NIR) DiFC to improve circulating cells' maximum detectable depth and SNR.
Phantom experiments were used to estimate the key parameters in a diffuse fluorescence excitation and emission model. This model and parameters were implemented in Monte Carlo to simulate DR DiFC while varying noise and AF parameters to identify the advantages and limitations of the proposed technique.
Two key factors must be true to give DR DiFC an advantage over traditional DiFC: first, the fraction of noise that DR methods cannot cancel cannot be above the order of 10% for acceptable SNR. Second, DR DiFC has an advantage, in terms of SNR, if the distribution of tissue AF contributors is surface-weighted.
DR cancelable noise may be designed (e.g., through the use of source multiplexing), and indications point to the AF contributors' distribution being truly surface-weighted
. Successful and worthwhile implementation of DR DiFC depends on these considerations, but results point to DR DiFC having possible advantages over traditional DiFC.</description><subject>Background noise</subject><subject>Blood</subject><subject>Blood vessels</subject><subject>Cancer</subject><subject>Chemical compounds</subject><subject>Flow cytometry</subject><subject>Fluorescence</subject><subject>Fluorophores</subject><subject>In vivo methods and tests</subject><subject>Mathematical models</subject><subject>Measurement methods</subject><subject>Medical prognosis</subject><subject>Medical research</subject><subject>Multiplexing</subject><subject>Optical measurement</subject><subject>Parameter identification</subject><subject>Phantoms, Imaging</subject><subject>Sensing</subject><subject>Sensors</subject><subject>Signal to noise ratio</subject><subject>Tissues</subject><subject>Veins & arteries</subject><issn>1083-3668</issn><issn>1560-2281</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkc1rFTEUxUNR-mX_gG4k4MbNjLn5vLMSW6utPOjGrkOayfSlzJuMyYzgf2_ktUVdhISb3zncwyHkHFgLAOYDtN8ubluOrWmZMYzBATkGpVnDOcKr-mYoGqE1HpGTUh4ZY6g7fUiOhJEoO2OOyebz6sYmuyUm6uY5J-e3dEiZ9mEJvk4nmgY6pzgtdBjXlNO8TTkUGid6H9OYHqJ3I11iKWt4Q14Pbizh7Ok-JXdfrr5fXjeb2683l582jZccl6ZjzPEAWijlXd-DRKEQg1LCy65H5J0Qg-jq0VqLAIADd71m0nReyb4Xp-Tj3nde73eh92FashvtnOPO5V82uWj__Zni1j6knxaY0BwZVIf3Tw45_VhDWewuFh_G0U0hrcVylCAZV4AVffcf-pjWPNV8lVIoDKLUlYI95XMqJYfhZRtg9k9ZFmwtq0qssfuyqubt3zFeFM_tiN9mno-m</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Blaney, Giles</creator><creator>Ivich, Fernando</creator><creator>Sassaroli, Angelo</creator><creator>Niedre, Mark</creator><creator>Fantini, Sergio</creator><general>S P I E - International Society for</general><general>Society of Photo-Optical Instrumentation Engineers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M7P</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-5116-525X</orcidid><orcidid>https://orcid.org/0000-0003-2830-3161</orcidid><orcidid>https://orcid.org/0000-0003-3419-4547</orcidid></search><sort><creationdate>20230701</creationdate><title>Dual-ratio approach for detection of point fluorophores in biological tissue</title><author>Blaney, Giles ; 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flow cytometry (DiFC) is an emerging fluorescence sensing method to non-invasively detect labeled circulating cells
. However, due to signal-to-noise ratio (SNR) constraints largely attributed to background tissue autofluorescence (AF), DiFC's measurement depth is limited.
The dual ratio (DR)/dual slope is an optical measurement method that aims to suppress noise and enhance SNR to deep tissue regions. We aim to investigate the combination of DR and near-infrared (NIR) DiFC to improve circulating cells' maximum detectable depth and SNR.
Phantom experiments were used to estimate the key parameters in a diffuse fluorescence excitation and emission model. This model and parameters were implemented in Monte Carlo to simulate DR DiFC while varying noise and AF parameters to identify the advantages and limitations of the proposed technique.
Two key factors must be true to give DR DiFC an advantage over traditional DiFC: first, the fraction of noise that DR methods cannot cancel cannot be above the order of 10% for acceptable SNR. Second, DR DiFC has an advantage, in terms of SNR, if the distribution of tissue AF contributors is surface-weighted.
DR cancelable noise may be designed (e.g., through the use of source multiplexing), and indications point to the AF contributors' distribution being truly surface-weighted
. Successful and worthwhile implementation of DR DiFC depends on these considerations, but results point to DR DiFC having possible advantages over traditional DiFC.</abstract><cop>United States</cop><pub>S P I E - International Society for</pub><pmid>37484977</pmid><doi>10.1117/1.JBO.28.7.077001</doi><orcidid>https://orcid.org/0000-0001-5116-525X</orcidid><orcidid>https://orcid.org/0000-0003-2830-3161</orcidid><orcidid>https://orcid.org/0000-0003-3419-4547</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Background noise Blood Blood vessels Cancer Chemical compounds Flow cytometry Fluorescence Fluorophores In vivo methods and tests Mathematical models Measurement methods Medical prognosis Medical research Multiplexing Optical measurement Parameter identification Phantoms, Imaging Sensing Sensors Signal to noise ratio Tissues Veins & arteries |
| title | Dual-ratio approach for detection of point fluorophores in biological tissue |
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