Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis
Immunosenescence and inflammaging facilitate the insurgence of chronic diseases. The Mediterranean diet is a non-invasive intervention to improve the chronic low-grade inflammatory status associated with aging. Olive oil oleuropein (OLE) and hydroxytyrosol (HT) demonstrated a controversial modulator...
Gespeichert in:
| Veröffentlicht in: | International journal of molecular sciences 2023-07, Vol.24 (13), p.11029 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 13 |
| container_start_page | 11029 |
| container_title | International journal of molecular sciences |
| container_volume | 24 |
| creator | Pojero, Fanny Gervasi, Francesco Fiore, Salvatore Davide Aiello, Anna Bonacci, Sonia Caldarella, Rosalia Attanzio, Alessandro Candore, Giuseppina Caruso, Calogero Ligotti, Mattia Emanuela Procopio, Antonio Restivo, Ignazio Tesoriere, Luisa Allegra, Mario Accardi, Giulia |
| description | Immunosenescence and inflammaging facilitate the insurgence of chronic diseases. The Mediterranean diet is a non-invasive intervention to improve the chronic low-grade inflammatory status associated with aging. Olive oil oleuropein (OLE) and hydroxytyrosol (HT) demonstrated a controversial modulatory action on inflammation in vitro when tested at concentrations exceeding those detectable in human plasma. We studied the potential anti-inflammatory effects of OLE and HT at nutritionally relevant concentrations on peripheral blood mononuclear cells (PBMCs) as regards cell viability, frequency of leukocyte subsets, and cytokine release, performing an age-focused analysis on two groups of subjects: Adult (age 18-64 years) and Senior (age ≥ 65 years). OLE and HT were used alone or as a pre-treatment before challenging PBMCs with lipopolysaccharide (LPS). Both polyphenols had no effect on cell viability irrespective of LPS, but 5 µM HT had an LPS-like effect on monocytes, reducing the intermediate subset in Adult subjects. OLE and HT had no effect on LPS-triggered release of TNF-α, IL-6 and IL-8, but 5 µM HT reduced IL-10 secretion by PBMCs from Adult vs. Senior group. In summary, nutritionally relevant concentrations of OLE and HT elicit no anti-inflammatory effect and influence the frequency of immune cell subsets with age-related different outcomes. |
| doi_str_mv | 10.3390/ijms241311029 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10341641</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A758317765</galeid><sourcerecordid>A758317765</sourcerecordid><originalsourceid>FETCH-LOGICAL-c483t-a0a91f77b09056b521cf7cddbd7e6da3da2534e792c703b2e1a95633ff4485c03</originalsourceid><addsrcrecordid>eNptkk1v1DAQhiMEomXhyBVZ4sIlxV-JEy4orAqtVChCcI68znjrlWNvbacif4dfirdbShchH_wxz7yj1zNF8ZLgE8Za_NZsxkg5YYRg2j4qjgmntMS4Fo8fnI-KZzFuMKaMVu3T4ogJzmuK6-PiV-eSKc-dtnIcZfJhRqdag0oReY2-TCmYZLyT1s7oG1i4kS6hpXcKXApyF7oFLy1MwW_BOCTdgM7mIfifc5qDj94i79BXCGZ7BUFa9MF6P6DP3nk3KQsyoCVYG9-hzqFuDWUuIxMM-SrtHE18XjzR0kZ4cbcvih8fT78vz8qLy0_ny-6iVLxhqZRYtkQLscItrupVRYnSQg3DahBQD5INklaMg2ipEpitKBDZVjVjWnPeVAqzRfF-r7udViMMe4e23wYzyjD3Xpr-MOLMVb_2Nz3BjJM692BRvLlTCP56gpj60USVzUkHfoo9bVhDc0-aKqOv_0E3fgrZ8S1V85pxTP5Sa2mhN077XFjtRPtOVA0jQtQ7rZP_UHkNMBrlHWiT3w8Syn2Cyv2JAfS9SYL73VT1B1OV-VcPf-ae_jNG7DchYMrm</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2836463401</pqid></control><display><type>article</type><title>Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis</title><source>MDPI - Multidisciplinary Digital Publishing Institute</source><source>MEDLINE</source><source>PubMed Central (PMC)</source><source>EZB Electronic Journals Library</source><creator>Pojero, Fanny ; Gervasi, Francesco ; Fiore, Salvatore Davide ; Aiello, Anna ; Bonacci, Sonia ; Caldarella, Rosalia ; Attanzio, Alessandro ; Candore, Giuseppina ; Caruso, Calogero ; Ligotti, Mattia Emanuela ; Procopio, Antonio ; Restivo, Ignazio ; Tesoriere, Luisa ; Allegra, Mario ; Accardi, Giulia</creator><creatorcontrib>Pojero, Fanny ; Gervasi, Francesco ; Fiore, Salvatore Davide ; Aiello, Anna ; Bonacci, Sonia ; Caldarella, Rosalia ; Attanzio, Alessandro ; Candore, Giuseppina ; Caruso, Calogero ; Ligotti, Mattia Emanuela ; Procopio, Antonio ; Restivo, Ignazio ; Tesoriere, Luisa ; Allegra, Mario ; Accardi, Giulia</creatorcontrib><description>Immunosenescence and inflammaging facilitate the insurgence of chronic diseases. The Mediterranean diet is a non-invasive intervention to improve the chronic low-grade inflammatory status associated with aging. Olive oil oleuropein (OLE) and hydroxytyrosol (HT) demonstrated a controversial modulatory action on inflammation in vitro when tested at concentrations exceeding those detectable in human plasma. We studied the potential anti-inflammatory effects of OLE and HT at nutritionally relevant concentrations on peripheral blood mononuclear cells (PBMCs) as regards cell viability, frequency of leukocyte subsets, and cytokine release, performing an age-focused analysis on two groups of subjects: Adult (age 18-64 years) and Senior (age ≥ 65 years). OLE and HT were used alone or as a pre-treatment before challenging PBMCs with lipopolysaccharide (LPS). Both polyphenols had no effect on cell viability irrespective of LPS, but 5 µM HT had an LPS-like effect on monocytes, reducing the intermediate subset in Adult subjects. OLE and HT had no effect on LPS-triggered release of TNF-α, IL-6 and IL-8, but 5 µM HT reduced IL-10 secretion by PBMCs from Adult vs. Senior group. In summary, nutritionally relevant concentrations of OLE and HT elicit no anti-inflammatory effect and influence the frequency of immune cell subsets with age-related different outcomes.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms241311029</identifier><identifier>PMID: 37446206</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Adolescent ; Adult ; Age ; Aged ; Aging ; Analysis ; Anti-inflammatory drugs ; Blood plasma ; Cell viability ; Chronic diseases ; Chronic illnesses ; Cytokines ; Homeostasis ; Humans ; Immune system ; Immunosenescence ; Inflammation ; Interleukin 10 ; Interleukin 6 ; Interleukin 8 ; Leukocytes ; Leukocytes, Mononuclear ; Lipopolysaccharides ; Lipopolysaccharides - toxicity ; Lymphocytes ; Middle Aged ; Monocytes ; Oleuropein ; Olive oil ; Peripheral blood mononuclear cells ; Phenylethyl Alcohol - pharmacology ; Polyphenols ; Polyphenols - pharmacology ; Statistical analysis ; Statistical significance ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Young Adult</subject><ispartof>International journal of molecular sciences, 2023-07, Vol.24 (13), p.11029</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-a0a91f77b09056b521cf7cddbd7e6da3da2534e792c703b2e1a95633ff4485c03</citedby><cites>FETCH-LOGICAL-c483t-a0a91f77b09056b521cf7cddbd7e6da3da2534e792c703b2e1a95633ff4485c03</cites><orcidid>0000-0002-6622-028X ; 0000-0001-8004-2363 ; 0000-0002-4971-240X ; 0000-0002-9052-7206 ; 0000-0002-5846-1868 ; 0000-0002-7980-7530 ; 0000-0003-1833-2356 ; 0000-0002-9966-934X ; 0000-0003-2593-3221 ; 0000-0003-3565-9529 ; 0000-0003-0540-1025</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341641/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341641/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37446206$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pojero, Fanny</creatorcontrib><creatorcontrib>Gervasi, Francesco</creatorcontrib><creatorcontrib>Fiore, Salvatore Davide</creatorcontrib><creatorcontrib>Aiello, Anna</creatorcontrib><creatorcontrib>Bonacci, Sonia</creatorcontrib><creatorcontrib>Caldarella, Rosalia</creatorcontrib><creatorcontrib>Attanzio, Alessandro</creatorcontrib><creatorcontrib>Candore, Giuseppina</creatorcontrib><creatorcontrib>Caruso, Calogero</creatorcontrib><creatorcontrib>Ligotti, Mattia Emanuela</creatorcontrib><creatorcontrib>Procopio, Antonio</creatorcontrib><creatorcontrib>Restivo, Ignazio</creatorcontrib><creatorcontrib>Tesoriere, Luisa</creatorcontrib><creatorcontrib>Allegra, Mario</creatorcontrib><creatorcontrib>Accardi, Giulia</creatorcontrib><title>Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>Immunosenescence and inflammaging facilitate the insurgence of chronic diseases. The Mediterranean diet is a non-invasive intervention to improve the chronic low-grade inflammatory status associated with aging. Olive oil oleuropein (OLE) and hydroxytyrosol (HT) demonstrated a controversial modulatory action on inflammation in vitro when tested at concentrations exceeding those detectable in human plasma. We studied the potential anti-inflammatory effects of OLE and HT at nutritionally relevant concentrations on peripheral blood mononuclear cells (PBMCs) as regards cell viability, frequency of leukocyte subsets, and cytokine release, performing an age-focused analysis on two groups of subjects: Adult (age 18-64 years) and Senior (age ≥ 65 years). OLE and HT were used alone or as a pre-treatment before challenging PBMCs with lipopolysaccharide (LPS). Both polyphenols had no effect on cell viability irrespective of LPS, but 5 µM HT had an LPS-like effect on monocytes, reducing the intermediate subset in Adult subjects. OLE and HT had no effect on LPS-triggered release of TNF-α, IL-6 and IL-8, but 5 µM HT reduced IL-10 secretion by PBMCs from Adult vs. Senior group. In summary, nutritionally relevant concentrations of OLE and HT elicit no anti-inflammatory effect and influence the frequency of immune cell subsets with age-related different outcomes.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Age</subject><subject>Aged</subject><subject>Aging</subject><subject>Analysis</subject><subject>Anti-inflammatory drugs</subject><subject>Blood plasma</subject><subject>Cell viability</subject><subject>Chronic diseases</subject><subject>Chronic illnesses</subject><subject>Cytokines</subject><subject>Homeostasis</subject><subject>Humans</subject><subject>Immune system</subject><subject>Immunosenescence</subject><subject>Inflammation</subject><subject>Interleukin 10</subject><subject>Interleukin 6</subject><subject>Interleukin 8</subject><subject>Leukocytes</subject><subject>Leukocytes, Mononuclear</subject><subject>Lipopolysaccharides</subject><subject>Lipopolysaccharides - toxicity</subject><subject>Lymphocytes</subject><subject>Middle Aged</subject><subject>Monocytes</subject><subject>Oleuropein</subject><subject>Olive oil</subject><subject>Peripheral blood mononuclear cells</subject><subject>Phenylethyl Alcohol - pharmacology</subject><subject>Polyphenols</subject><subject>Polyphenols - pharmacology</subject><subject>Statistical analysis</subject><subject>Statistical significance</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>Young Adult</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkk1v1DAQhiMEomXhyBVZ4sIlxV-JEy4orAqtVChCcI68znjrlWNvbacif4dfirdbShchH_wxz7yj1zNF8ZLgE8Za_NZsxkg5YYRg2j4qjgmntMS4Fo8fnI-KZzFuMKaMVu3T4ogJzmuK6-PiV-eSKc-dtnIcZfJhRqdag0oReY2-TCmYZLyT1s7oG1i4kS6hpXcKXApyF7oFLy1MwW_BOCTdgM7mIfifc5qDj94i79BXCGZ7BUFa9MF6P6DP3nk3KQsyoCVYG9-hzqFuDWUuIxMM-SrtHE18XjzR0kZ4cbcvih8fT78vz8qLy0_ny-6iVLxhqZRYtkQLscItrupVRYnSQg3DahBQD5INklaMg2ipEpitKBDZVjVjWnPeVAqzRfF-r7udViMMe4e23wYzyjD3Xpr-MOLMVb_2Nz3BjJM692BRvLlTCP56gpj60USVzUkHfoo9bVhDc0-aKqOv_0E3fgrZ8S1V85pxTP5Sa2mhN077XFjtRPtOVA0jQtQ7rZP_UHkNMBrlHWiT3w8Syn2Cyv2JAfS9SYL73VT1B1OV-VcPf-ae_jNG7DchYMrm</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Pojero, Fanny</creator><creator>Gervasi, Francesco</creator><creator>Fiore, Salvatore Davide</creator><creator>Aiello, Anna</creator><creator>Bonacci, Sonia</creator><creator>Caldarella, Rosalia</creator><creator>Attanzio, Alessandro</creator><creator>Candore, Giuseppina</creator><creator>Caruso, Calogero</creator><creator>Ligotti, Mattia Emanuela</creator><creator>Procopio, Antonio</creator><creator>Restivo, Ignazio</creator><creator>Tesoriere, Luisa</creator><creator>Allegra, Mario</creator><creator>Accardi, Giulia</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-6622-028X</orcidid><orcidid>https://orcid.org/0000-0001-8004-2363</orcidid><orcidid>https://orcid.org/0000-0002-4971-240X</orcidid><orcidid>https://orcid.org/0000-0002-9052-7206</orcidid><orcidid>https://orcid.org/0000-0002-5846-1868</orcidid><orcidid>https://orcid.org/0000-0002-7980-7530</orcidid><orcidid>https://orcid.org/0000-0003-1833-2356</orcidid><orcidid>https://orcid.org/0000-0002-9966-934X</orcidid><orcidid>https://orcid.org/0000-0003-2593-3221</orcidid><orcidid>https://orcid.org/0000-0003-3565-9529</orcidid><orcidid>https://orcid.org/0000-0003-0540-1025</orcidid></search><sort><creationdate>20230701</creationdate><title>Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis</title><author>Pojero, Fanny ; Gervasi, Francesco ; Fiore, Salvatore Davide ; Aiello, Anna ; Bonacci, Sonia ; Caldarella, Rosalia ; Attanzio, Alessandro ; Candore, Giuseppina ; Caruso, Calogero ; Ligotti, Mattia Emanuela ; Procopio, Antonio ; Restivo, Ignazio ; Tesoriere, Luisa ; Allegra, Mario ; Accardi, Giulia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-a0a91f77b09056b521cf7cddbd7e6da3da2534e792c703b2e1a95633ff4485c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Age</topic><topic>Aged</topic><topic>Aging</topic><topic>Analysis</topic><topic>Anti-inflammatory drugs</topic><topic>Blood plasma</topic><topic>Cell viability</topic><topic>Chronic diseases</topic><topic>Chronic illnesses</topic><topic>Cytokines</topic><topic>Homeostasis</topic><topic>Humans</topic><topic>Immune system</topic><topic>Immunosenescence</topic><topic>Inflammation</topic><topic>Interleukin 10</topic><topic>Interleukin 6</topic><topic>Interleukin 8</topic><topic>Leukocytes</topic><topic>Leukocytes, Mononuclear</topic><topic>Lipopolysaccharides</topic><topic>Lipopolysaccharides - toxicity</topic><topic>Lymphocytes</topic><topic>Middle Aged</topic><topic>Monocytes</topic><topic>Oleuropein</topic><topic>Olive oil</topic><topic>Peripheral blood mononuclear cells</topic><topic>Phenylethyl Alcohol - pharmacology</topic><topic>Polyphenols</topic><topic>Polyphenols - pharmacology</topic><topic>Statistical analysis</topic><topic>Statistical significance</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pojero, Fanny</creatorcontrib><creatorcontrib>Gervasi, Francesco</creatorcontrib><creatorcontrib>Fiore, Salvatore Davide</creatorcontrib><creatorcontrib>Aiello, Anna</creatorcontrib><creatorcontrib>Bonacci, Sonia</creatorcontrib><creatorcontrib>Caldarella, Rosalia</creatorcontrib><creatorcontrib>Attanzio, Alessandro</creatorcontrib><creatorcontrib>Candore, Giuseppina</creatorcontrib><creatorcontrib>Caruso, Calogero</creatorcontrib><creatorcontrib>Ligotti, Mattia Emanuela</creatorcontrib><creatorcontrib>Procopio, Antonio</creatorcontrib><creatorcontrib>Restivo, Ignazio</creatorcontrib><creatorcontrib>Tesoriere, Luisa</creatorcontrib><creatorcontrib>Allegra, Mario</creatorcontrib><creatorcontrib>Accardi, Giulia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest research library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of molecular sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pojero, Fanny</au><au>Gervasi, Francesco</au><au>Fiore, Salvatore Davide</au><au>Aiello, Anna</au><au>Bonacci, Sonia</au><au>Caldarella, Rosalia</au><au>Attanzio, Alessandro</au><au>Candore, Giuseppina</au><au>Caruso, Calogero</au><au>Ligotti, Mattia Emanuela</au><au>Procopio, Antonio</au><au>Restivo, Ignazio</au><au>Tesoriere, Luisa</au><au>Allegra, Mario</au><au>Accardi, Giulia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis</atitle><jtitle>International journal of molecular sciences</jtitle><addtitle>Int J Mol Sci</addtitle><date>2023-07-01</date><risdate>2023</risdate><volume>24</volume><issue>13</issue><spage>11029</spage><pages>11029-</pages><issn>1422-0067</issn><issn>1661-6596</issn><eissn>1422-0067</eissn><abstract>Immunosenescence and inflammaging facilitate the insurgence of chronic diseases. The Mediterranean diet is a non-invasive intervention to improve the chronic low-grade inflammatory status associated with aging. Olive oil oleuropein (OLE) and hydroxytyrosol (HT) demonstrated a controversial modulatory action on inflammation in vitro when tested at concentrations exceeding those detectable in human plasma. We studied the potential anti-inflammatory effects of OLE and HT at nutritionally relevant concentrations on peripheral blood mononuclear cells (PBMCs) as regards cell viability, frequency of leukocyte subsets, and cytokine release, performing an age-focused analysis on two groups of subjects: Adult (age 18-64 years) and Senior (age ≥ 65 years). OLE and HT were used alone or as a pre-treatment before challenging PBMCs with lipopolysaccharide (LPS). Both polyphenols had no effect on cell viability irrespective of LPS, but 5 µM HT had an LPS-like effect on monocytes, reducing the intermediate subset in Adult subjects. OLE and HT had no effect on LPS-triggered release of TNF-α, IL-6 and IL-8, but 5 µM HT reduced IL-10 secretion by PBMCs from Adult vs. Senior group. In summary, nutritionally relevant concentrations of OLE and HT elicit no anti-inflammatory effect and influence the frequency of immune cell subsets with age-related different outcomes.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>37446206</pmid><doi>10.3390/ijms241311029</doi><orcidid>https://orcid.org/0000-0002-6622-028X</orcidid><orcidid>https://orcid.org/0000-0001-8004-2363</orcidid><orcidid>https://orcid.org/0000-0002-4971-240X</orcidid><orcidid>https://orcid.org/0000-0002-9052-7206</orcidid><orcidid>https://orcid.org/0000-0002-5846-1868</orcidid><orcidid>https://orcid.org/0000-0002-7980-7530</orcidid><orcidid>https://orcid.org/0000-0003-1833-2356</orcidid><orcidid>https://orcid.org/0000-0002-9966-934X</orcidid><orcidid>https://orcid.org/0000-0003-2593-3221</orcidid><orcidid>https://orcid.org/0000-0003-3565-9529</orcidid><orcidid>https://orcid.org/0000-0003-0540-1025</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1422-0067 |
| ispartof | International journal of molecular sciences, 2023-07, Vol.24 (13), p.11029 |
| issn | 1422-0067 1661-6596 1422-0067 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10341641 |
| source | MDPI - Multidisciplinary Digital Publishing Institute; MEDLINE; PubMed Central (PMC); EZB Electronic Journals Library |
| subjects | Adolescent Adult Age Aged Aging Analysis Anti-inflammatory drugs Blood plasma Cell viability Chronic diseases Chronic illnesses Cytokines Homeostasis Humans Immune system Immunosenescence Inflammation Interleukin 10 Interleukin 6 Interleukin 8 Leukocytes Leukocytes, Mononuclear Lipopolysaccharides Lipopolysaccharides - toxicity Lymphocytes Middle Aged Monocytes Oleuropein Olive oil Peripheral blood mononuclear cells Phenylethyl Alcohol - pharmacology Polyphenols Polyphenols - pharmacology Statistical analysis Statistical significance Tumor necrosis factor-TNF Tumor necrosis factor-α Young Adult |
| title | Anti-Inflammatory Effects of Nutritionally Relevant Concentrations of Oleuropein and Hydroxytyrosol on Peripheral Blood Mononuclear Cells: An Age-Related Analysis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T14%3A18%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Anti-Inflammatory%20Effects%20of%20Nutritionally%20Relevant%20Concentrations%20of%20Oleuropein%20and%20Hydroxytyrosol%20on%20Peripheral%20Blood%20Mononuclear%20Cells:%20An%20Age-Related%20Analysis&rft.jtitle=International%20journal%20of%20molecular%20sciences&rft.au=Pojero,%20Fanny&rft.date=2023-07-01&rft.volume=24&rft.issue=13&rft.spage=11029&rft.pages=11029-&rft.issn=1422-0067&rft.eissn=1422-0067&rft_id=info:doi/10.3390/ijms241311029&rft_dat=%3Cgale_pubme%3EA758317765%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2836463401&rft_id=info:pmid/37446206&rft_galeid=A758317765&rfr_iscdi=true |