The Cuprizone Mouse Model: A Comparative Study of Cuprizone Formulations from Different Manufacturers
The Cuprizone mouse model is widely used in studies on de- and remyelination. In the hands of different experimenters, the Cuprizone concentrations that lead to comparable levels of demyelination differ considerably. The reasons for this variability are unknown. In this study, we tested whether diff...
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| Veröffentlicht in: | International journal of molecular sciences 2023-06, Vol.24 (13), p.10564 |
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| creator | Beecken, Malena Baumann, Louise Vankriekelsvenne, Elise Manzhula, Katerina Greiner, Theresa Heinig, Leo Schauerte, Steffen Kipp, Markus Joost, Sarah |
| description | The Cuprizone mouse model is widely used in studies on de- and remyelination. In the hands of different experimenters, the Cuprizone concentrations that lead to comparable levels of demyelination differ considerably. The reasons for this variability are unknown. In this study, we tested whether different Cuprizone formulations from different vendors and manufacturers influenced Cuprizone-induced histopathological hallmarks. We intoxicated male C57BL/6 mice with six Cuprizone powders that differed in their manufacturer, vendor, and purity. After five weeks, we analyzed the body weight changes over the course of the experiment, as well as the demyelination, astrogliosis, microgliosis and axonal damage by histological LFB-PAS staining and immunohistochemical labelling of PLP, IBA1, GFAP and APP. All Cuprizone formulations induced demyelination, astrogliosis, microgliosis, axonal damage and a moderate drop in body weight at the beginning of the intoxication period. In a cumulative evaluation of all analyses, two Cuprizone formulations performed weaker than the other formulations. In conclusion, all tested formulations did work, but the choice of Cuprizone formulation may have been responsible for the considerable variability in the experimental outcomes. |
| doi_str_mv | 10.3390/ijms241310564 |
| format | Article |
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In the hands of different experimenters, the Cuprizone concentrations that lead to comparable levels of demyelination differ considerably. The reasons for this variability are unknown. In this study, we tested whether different Cuprizone formulations from different vendors and manufacturers influenced Cuprizone-induced histopathological hallmarks. We intoxicated male C57BL/6 mice with six Cuprizone powders that differed in their manufacturer, vendor, and purity. After five weeks, we analyzed the body weight changes over the course of the experiment, as well as the demyelination, astrogliosis, microgliosis and axonal damage by histological LFB-PAS staining and immunohistochemical labelling of PLP, IBA1, GFAP and APP. All Cuprizone formulations induced demyelination, astrogliosis, microgliosis, axonal damage and a moderate drop in body weight at the beginning of the intoxication period. In a cumulative evaluation of all analyses, two Cuprizone formulations performed weaker than the other formulations. In conclusion, all tested formulations did work, but the choice of Cuprizone formulation may have been responsible for the considerable variability in the experimental outcomes.</description><identifier>ISSN: 1422-0067</identifier><identifier>ISSN: 1661-6596</identifier><identifier>EISSN: 1422-0067</identifier><identifier>DOI: 10.3390/ijms241310564</identifier><identifier>PMID: 37445742</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Analysis ; Animals ; Apoptosis ; Body Weight ; Comparative studies ; Copper ; Cuprizone ; Cuprizone - toxicity ; Demyelinating Diseases - chemically induced ; Demyelinating Diseases - pathology ; Demyelination ; Disease Models, Animal ; Glial fibrillary acidic protein ; Gliosis ; Intoxication ; Labeling ; Male ; Mice ; Mice, Inbred C57BL ; Myelin proteolipid protein ; Myelin Sheath - pathology ; Myelination ; Variability</subject><ispartof>International journal of molecular sciences, 2023-06, Vol.24 (13), p.10564</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c439t-fa24ef5d010cf60fb4bb75e5f6ce7e5dacee3fd64f4c943c9818f721cec3cc673</cites><orcidid>0000-0001-8654-8904 ; 0000-0001-5043-9052 ; 0000-0001-5549-7534 ; 0000-0003-4052-7125</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341492/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341492/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37445742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Beecken, Malena</creatorcontrib><creatorcontrib>Baumann, Louise</creatorcontrib><creatorcontrib>Vankriekelsvenne, Elise</creatorcontrib><creatorcontrib>Manzhula, Katerina</creatorcontrib><creatorcontrib>Greiner, Theresa</creatorcontrib><creatorcontrib>Heinig, Leo</creatorcontrib><creatorcontrib>Schauerte, Steffen</creatorcontrib><creatorcontrib>Kipp, Markus</creatorcontrib><creatorcontrib>Joost, Sarah</creatorcontrib><title>The Cuprizone Mouse Model: A Comparative Study of Cuprizone Formulations from Different Manufacturers</title><title>International journal of molecular sciences</title><addtitle>Int J Mol Sci</addtitle><description>The Cuprizone mouse model is widely used in studies on de- and remyelination. In the hands of different experimenters, the Cuprizone concentrations that lead to comparable levels of demyelination differ considerably. The reasons for this variability are unknown. In this study, we tested whether different Cuprizone formulations from different vendors and manufacturers influenced Cuprizone-induced histopathological hallmarks. We intoxicated male C57BL/6 mice with six Cuprizone powders that differed in their manufacturer, vendor, and purity. After five weeks, we analyzed the body weight changes over the course of the experiment, as well as the demyelination, astrogliosis, microgliosis and axonal damage by histological LFB-PAS staining and immunohistochemical labelling of PLP, IBA1, GFAP and APP. All Cuprizone formulations induced demyelination, astrogliosis, microgliosis, axonal damage and a moderate drop in body weight at the beginning of the intoxication period. In a cumulative evaluation of all analyses, two Cuprizone formulations performed weaker than the other formulations. In conclusion, all tested formulations did work, but the choice of Cuprizone formulation may have been responsible for the considerable variability in the experimental outcomes.</description><subject>Analysis</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Body Weight</subject><subject>Comparative studies</subject><subject>Copper</subject><subject>Cuprizone</subject><subject>Cuprizone - toxicity</subject><subject>Demyelinating Diseases - chemically induced</subject><subject>Demyelinating Diseases - pathology</subject><subject>Demyelination</subject><subject>Disease Models, Animal</subject><subject>Glial fibrillary acidic protein</subject><subject>Gliosis</subject><subject>Intoxication</subject><subject>Labeling</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Myelin proteolipid protein</subject><subject>Myelin Sheath - pathology</subject><subject>Myelination</subject><subject>Variability</subject><issn>1422-0067</issn><issn>1661-6596</issn><issn>1422-0067</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNptkk1v1DAQhi0EoqVw5IosceklxR-TOOGCVgttkVpxoJwtrzNuvUrixY4rlV-Ply7VLkKWbMvzzOuZV0PIW87OpOzYB78ekwAuOasbeEaOOQhRMdao53v3I_IqpTVjQoq6e0mOpAKoFYhjgjd3SJd5E_2vMCG9Djlt9x6Hj3RBl2HcmGhmf4_0-5z7BxrcHn0e4piHEg5Toi6GkX72zmHEaabXZsrO2DlHjOk1eeHMkPDN7jwhP86_3Cwvq6tvF1-Xi6vKguzmyhkB6OqecWZdw9wKVitVY-0aiwrr3lhE6foGHNgOpO1a3joluEUrrW2UPCGfHnU3eTVib0sh0Qy61Dua-KCD8fowMvk7fRvuNWcSOHSiKJzuFGL4mTHNevTJ4jCYCYs3WrSyFdAC4wV9_w-6DjlOpb8t1UAN4o_gjro1A2o_uVA-tltRvVB1K7kSXVuos_9QZfU4elu8dr68HyRUjwk2hpQiuqcmOdPbwdAHg1H4d_vOPNF_J0H-BpK_tTQ</recordid><startdate>20230623</startdate><enddate>20230623</enddate><creator>Beecken, Malena</creator><creator>Baumann, Louise</creator><creator>Vankriekelsvenne, Elise</creator><creator>Manzhula, Katerina</creator><creator>Greiner, Theresa</creator><creator>Heinig, Leo</creator><creator>Schauerte, Steffen</creator><creator>Kipp, Markus</creator><creator>Joost, Sarah</creator><general>MDPI AG</general><general>MDPI</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-8654-8904</orcidid><orcidid>https://orcid.org/0000-0001-5043-9052</orcidid><orcidid>https://orcid.org/0000-0001-5549-7534</orcidid><orcidid>https://orcid.org/0000-0003-4052-7125</orcidid></search><sort><creationdate>20230623</creationdate><title>The Cuprizone Mouse Model: A Comparative Study of Cuprizone Formulations from Different Manufacturers</title><author>Beecken, Malena ; 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In the hands of different experimenters, the Cuprizone concentrations that lead to comparable levels of demyelination differ considerably. The reasons for this variability are unknown. In this study, we tested whether different Cuprizone formulations from different vendors and manufacturers influenced Cuprizone-induced histopathological hallmarks. We intoxicated male C57BL/6 mice with six Cuprizone powders that differed in their manufacturer, vendor, and purity. After five weeks, we analyzed the body weight changes over the course of the experiment, as well as the demyelination, astrogliosis, microgliosis and axonal damage by histological LFB-PAS staining and immunohistochemical labelling of PLP, IBA1, GFAP and APP. All Cuprizone formulations induced demyelination, astrogliosis, microgliosis, axonal damage and a moderate drop in body weight at the beginning of the intoxication period. 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| subjects | Analysis Animals Apoptosis Body Weight Comparative studies Copper Cuprizone Cuprizone - toxicity Demyelinating Diseases - chemically induced Demyelinating Diseases - pathology Demyelination Disease Models, Animal Glial fibrillary acidic protein Gliosis Intoxication Labeling Male Mice Mice, Inbred C57BL Myelin proteolipid protein Myelin Sheath - pathology Myelination Variability |
| title | The Cuprizone Mouse Model: A Comparative Study of Cuprizone Formulations from Different Manufacturers |
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