Screening of volatile organic compounds (VOCs) from liquid fungal cultures using ambient mass spectrometry
The potential of fungi for use as biotechnological factories in the production of a range of valuable metabolites, such as enzymes, terpenes, and volatile aroma compounds, is high. Unlike other microorganisms, fungi mostly secrete secondary metabolites into the culture medium, allowing for easy extr...
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| Veröffentlicht in: | Analytical and bioanalytical chemistry 2023-07, Vol.415 (18), p.4615-4627 |
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| creator | Heffernan, Daniel Pilz, Melania Klein, Marco Haack, Martina Race, Alan M. Brück, Thomas Qoura, Farah Strittmatter, Nicole |
| description | The potential of fungi for use as biotechnological factories in the production of a range of valuable metabolites, such as enzymes, terpenes, and volatile aroma compounds, is high. Unlike other microorganisms, fungi mostly secrete secondary metabolites into the culture medium, allowing for easy extraction and analysis. To date, the most commonly used technique in the analysis of volatile organic compounds (VOCs) is gas chromatography, which is time and labour consuming. We propose an alternative ambient screening method that provides rapid chemical information for characterising the VOCs of filamentous fungi in liquid culture using a commercially available ambient dielectric barrier discharge ionisation (DBDI) source connected to a quadrupole-Orbitrap mass spectrometer. The effects of method parameters on measured peak intensities of a series of 8 selected aroma standards were optimised with the best conditions being selected for sample analysis. The developed method was then deployed to the screening of VOCs from samples of 13 fungal strains in three different types of complex growth media showing clear differences in VOC profiles across the different media, enabling determination of best culturing conditions for each compound-strain combination. Our findings underline the applicability of ambient DBDI for the direct detection and comparison of aroma compounds produced by filamentous fungi in liquid culture.
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| doi_str_mv | 10.1007/s00216-023-04769-6 |
| format | Article |
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Graphical abstract</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Aroma compounds</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Dielectric barrier discharge</subject><subject>Food Science</subject><subject>Fungi</subject><subject>Gas chromatography</subject><subject>Growth media</subject><subject>Identification and classification</subject><subject>Laboratory Medicine</subject><subject>Liquid culture</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolites</subject><subject>Methods</subject><subject>Microorganisms</subject><subject>Monitoring/Environmental Analysis</subject><subject>Organic compounds</subject><subject>Ozone</subject><subject>Quadrupoles</subject><subject>Research 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Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heffernan, Daniel</au><au>Pilz, Melania</au><au>Klein, Marco</au><au>Haack, Martina</au><au>Race, Alan M.</au><au>Brück, Thomas</au><au>Qoura, Farah</au><au>Strittmatter, Nicole</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening of volatile organic compounds (VOCs) from liquid fungal cultures using ambient mass spectrometry</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2023-07-01</date><risdate>2023</risdate><volume>415</volume><issue>18</issue><spage>4615</spage><epage>4627</epage><pages>4615-4627</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>The potential of fungi for use as biotechnological factories in the production of a range of valuable metabolites, such as enzymes, terpenes, and volatile aroma compounds, is high. Unlike other microorganisms, fungi mostly secrete secondary metabolites into the culture medium, allowing for easy extraction and analysis. To date, the most commonly used technique in the analysis of volatile organic compounds (VOCs) is gas chromatography, which is time and labour consuming. We propose an alternative ambient screening method that provides rapid chemical information for characterising the VOCs of filamentous fungi in liquid culture using a commercially available ambient dielectric barrier discharge ionisation (DBDI) source connected to a quadrupole-Orbitrap mass spectrometer. The effects of method parameters on measured peak intensities of a series of 8 selected aroma standards were optimised with the best conditions being selected for sample analysis. The developed method was then deployed to the screening of VOCs from samples of 13 fungal strains in three different types of complex growth media showing clear differences in VOC profiles across the different media, enabling determination of best culturing conditions for each compound-strain combination. Our findings underline the applicability of ambient DBDI for the direct detection and comparison of aroma compounds produced by filamentous fungi in liquid culture.
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| subjects | Analysis Analytical Chemistry Aroma compounds Biochemistry Biotechnology Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Dielectric barrier discharge Food Science Fungi Gas chromatography Growth media Identification and classification Laboratory Medicine Liquid culture Mass spectrometry Mass spectroscopy Metabolites Methods Microorganisms Monitoring/Environmental Analysis Organic compounds Ozone Quadrupoles Research Paper Screening Secondary metabolites Terpenes VOCs Volatile organic compounds Young Investigators in (Bio-)Analytical Chemistry 2023 |
| title | Screening of volatile organic compounds (VOCs) from liquid fungal cultures using ambient mass spectrometry |
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