The transcription factor PbrMYB24 regulates lignin and cellulose biosynthesis in stone cells of pear fruits
Abstract Lignified stone cell content is a key factor used to evaluate fruit quality, influencing the economic value of pear (Pyrus pyrifolia) fruits. However, our understanding of the regulatory networks of stone cell formation is limited due to the complex secondary metabolic pathway. In this stud...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2023-07, Vol.192 (3), p.1997-2014 |
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| container_title | Plant physiology (Bethesda) |
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| creator | Xue, Yongsong Shan, Yanfei Yao, Jia-Long Wang, Runze Xu, Shaozhuo Liu, Dongliang Ye, Zhicheng Lin, Jing Li, Xiaogang Xue, Cheng Wu, Jun |
| description | Abstract
Lignified stone cell content is a key factor used to evaluate fruit quality, influencing the economic value of pear (Pyrus pyrifolia) fruits. However, our understanding of the regulatory networks of stone cell formation is limited due to the complex secondary metabolic pathway. In this study, we used a combination of co-expression network analysis, gene expression profiles, and transcriptome analysis in different pear cultivars with varied stone cell content to identify a hub MYB gene, PbrMYB24. The relative expression of PbrMYB24 in fruit flesh was significantly correlated with the contents of stone cells, lignin, and cellulose. We then verified the function of PbrMYB24 in regulating lignin and cellulose formation via genetic transformation in homologous and heterologous systems. We constructed a high-efficiency verification system for lignin and cellulose biosynthesis genes in pear callus. PbrMYB24 transcriptionally activated multiple target genes involved in stone cell formation. On the one hand, PbrMYB24 activated the transcription of lignin and cellulose biosynthesis genes by binding to different cis-elements [AC-I (ACCTACC) element, AC-II (ACCAACC) element and MYB-binding sites (MBS)]. On the other hand, PbrMYB24 bound directly to the promoters of PbrMYB169 and NAC STONE CELL PROMOTING FACTOR (PbrNSC), activating the gene expression. Moreover, both PbrMYB169 and PbrNSC activated the promoter of PbrMYB24, enhancing gene expression. This study improves our understanding of lignin and cellulose synthesis regulation in pear fruits through identifying a regulator and establishing a regulatory network. This knowledge will be useful for reducing the stone cell content in pears via molecular breeding.
The transcription factor PbrMYB24 regulates lignin and cellulose biosynthesis in stone cells of pear fruits by binding to different cis-elements |
| doi_str_mv | 10.1093/plphys/kiad200 |
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Lignified stone cell content is a key factor used to evaluate fruit quality, influencing the economic value of pear (Pyrus pyrifolia) fruits. However, our understanding of the regulatory networks of stone cell formation is limited due to the complex secondary metabolic pathway. In this study, we used a combination of co-expression network analysis, gene expression profiles, and transcriptome analysis in different pear cultivars with varied stone cell content to identify a hub MYB gene, PbrMYB24. The relative expression of PbrMYB24 in fruit flesh was significantly correlated with the contents of stone cells, lignin, and cellulose. We then verified the function of PbrMYB24 in regulating lignin and cellulose formation via genetic transformation in homologous and heterologous systems. We constructed a high-efficiency verification system for lignin and cellulose biosynthesis genes in pear callus. PbrMYB24 transcriptionally activated multiple target genes involved in stone cell formation. On the one hand, PbrMYB24 activated the transcription of lignin and cellulose biosynthesis genes by binding to different cis-elements [AC-I (ACCTACC) element, AC-II (ACCAACC) element and MYB-binding sites (MBS)]. On the other hand, PbrMYB24 bound directly to the promoters of PbrMYB169 and NAC STONE CELL PROMOTING FACTOR (PbrNSC), activating the gene expression. Moreover, both PbrMYB169 and PbrNSC activated the promoter of PbrMYB24, enhancing gene expression. This study improves our understanding of lignin and cellulose synthesis regulation in pear fruits through identifying a regulator and establishing a regulatory network. This knowledge will be useful for reducing the stone cell content in pears via molecular breeding.
The transcription factor PbrMYB24 regulates lignin and cellulose biosynthesis in stone cells of pear fruits by binding to different cis-elements</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1093/plphys/kiad200</identifier><identifier>PMID: 37011145</identifier><language>eng</language><publisher>US: Oxford University Press</publisher><subject>Fruit - genetics ; Fruit - metabolism ; Gene Expression Regulation, Plant ; Lignin - metabolism ; Pyrus - genetics ; Pyrus - metabolism ; Transcription Factors - metabolism</subject><ispartof>Plant physiology (Bethesda), 2023-07, Vol.192 (3), p.1997-2014</ispartof><rights>American Society of Plant Biologists 2023. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2023</rights><rights>American Society of Plant Biologists 2023. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-c41ad4e51bc83395f86f0d94ea922e508ef806ee418bb2e248b24c018641bf5c3</citedby><cites>FETCH-LOGICAL-c430t-c41ad4e51bc83395f86f0d94ea922e508ef806ee418bb2e248b24c018641bf5c3</cites><orcidid>0000-0003-2570-8139 ; 0000-0002-8845-2719 ; 0000-0002-3541-9958 ; 0000-0002-1566-5115 ; 0009-0008-6315-1099</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,315,781,785,886,1585,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37011145$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xue, Yongsong</creatorcontrib><creatorcontrib>Shan, Yanfei</creatorcontrib><creatorcontrib>Yao, Jia-Long</creatorcontrib><creatorcontrib>Wang, Runze</creatorcontrib><creatorcontrib>Xu, Shaozhuo</creatorcontrib><creatorcontrib>Liu, Dongliang</creatorcontrib><creatorcontrib>Ye, Zhicheng</creatorcontrib><creatorcontrib>Lin, Jing</creatorcontrib><creatorcontrib>Li, Xiaogang</creatorcontrib><creatorcontrib>Xue, Cheng</creatorcontrib><creatorcontrib>Wu, Jun</creatorcontrib><title>The transcription factor PbrMYB24 regulates lignin and cellulose biosynthesis in stone cells of pear fruits</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Abstract
Lignified stone cell content is a key factor used to evaluate fruit quality, influencing the economic value of pear (Pyrus pyrifolia) fruits. However, our understanding of the regulatory networks of stone cell formation is limited due to the complex secondary metabolic pathway. In this study, we used a combination of co-expression network analysis, gene expression profiles, and transcriptome analysis in different pear cultivars with varied stone cell content to identify a hub MYB gene, PbrMYB24. The relative expression of PbrMYB24 in fruit flesh was significantly correlated with the contents of stone cells, lignin, and cellulose. We then verified the function of PbrMYB24 in regulating lignin and cellulose formation via genetic transformation in homologous and heterologous systems. We constructed a high-efficiency verification system for lignin and cellulose biosynthesis genes in pear callus. PbrMYB24 transcriptionally activated multiple target genes involved in stone cell formation. On the one hand, PbrMYB24 activated the transcription of lignin and cellulose biosynthesis genes by binding to different cis-elements [AC-I (ACCTACC) element, AC-II (ACCAACC) element and MYB-binding sites (MBS)]. On the other hand, PbrMYB24 bound directly to the promoters of PbrMYB169 and NAC STONE CELL PROMOTING FACTOR (PbrNSC), activating the gene expression. Moreover, both PbrMYB169 and PbrNSC activated the promoter of PbrMYB24, enhancing gene expression. This study improves our understanding of lignin and cellulose synthesis regulation in pear fruits through identifying a regulator and establishing a regulatory network. This knowledge will be useful for reducing the stone cell content in pears via molecular breeding.
The transcription factor PbrMYB24 regulates lignin and cellulose biosynthesis in stone cells of pear fruits by binding to different cis-elements</description><subject>Fruit - genetics</subject><subject>Fruit - metabolism</subject><subject>Gene Expression Regulation, Plant</subject><subject>Lignin - metabolism</subject><subject>Pyrus - genetics</subject><subject>Pyrus - metabolism</subject><subject>Transcription Factors - metabolism</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkTtvFDEUhS0URJZASxm5hGKT69fGrhCJeElBUISCyvJ4rnedzNqD7UHaf8-EXSKo0lxf6Xw-9tEh5BWDMwZGnI_DuNnV87voeg7whCyYEnzJldRHZAEw76C1OSbPa70FACaYfEaOxQUwxqRakLubDdJWXKq-xLHFnGhwvuVCv3Xly49LLmnB9TS4hpUOcZ1ioi711OMwTEOuSLuY6y61DdZY6azWlhP-0SvNgY7oCg1liq2-IE-DGyq-PJwn5PuH9zdXn5bXXz9-vnp3vfRSQJsnc71ExTqvhTAq6FWA3kh0hnNUoDFoWCFKpruOI5e649ID0yvJuqC8OCFv977j1G2x95jmgIMdS9y6srPZRfu_kuLGrvMvy0AwxY2ZHV4fHEr-OWFtdhvrfSSXME_V8gujhDKa8Rk926O-5FoLhod3GNj7iuy-InuoaL5w-u_vHvC_nczAmz2Qp_Exs9-j06A9</recordid><startdate>20230703</startdate><enddate>20230703</enddate><creator>Xue, Yongsong</creator><creator>Shan, Yanfei</creator><creator>Yao, Jia-Long</creator><creator>Wang, Runze</creator><creator>Xu, Shaozhuo</creator><creator>Liu, Dongliang</creator><creator>Ye, Zhicheng</creator><creator>Lin, Jing</creator><creator>Li, Xiaogang</creator><creator>Xue, Cheng</creator><creator>Wu, Jun</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-2570-8139</orcidid><orcidid>https://orcid.org/0000-0002-8845-2719</orcidid><orcidid>https://orcid.org/0000-0002-3541-9958</orcidid><orcidid>https://orcid.org/0000-0002-1566-5115</orcidid><orcidid>https://orcid.org/0009-0008-6315-1099</orcidid></search><sort><creationdate>20230703</creationdate><title>The transcription factor PbrMYB24 regulates lignin and cellulose biosynthesis in stone cells of pear fruits</title><author>Xue, Yongsong ; Shan, Yanfei ; Yao, Jia-Long ; Wang, Runze ; Xu, Shaozhuo ; Liu, Dongliang ; Ye, Zhicheng ; Lin, Jing ; Li, Xiaogang ; Xue, Cheng ; Wu, Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-c41ad4e51bc83395f86f0d94ea922e508ef806ee418bb2e248b24c018641bf5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Fruit - genetics</topic><topic>Fruit - metabolism</topic><topic>Gene Expression Regulation, Plant</topic><topic>Lignin - metabolism</topic><topic>Pyrus - genetics</topic><topic>Pyrus - metabolism</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xue, Yongsong</creatorcontrib><creatorcontrib>Shan, Yanfei</creatorcontrib><creatorcontrib>Yao, Jia-Long</creatorcontrib><creatorcontrib>Wang, Runze</creatorcontrib><creatorcontrib>Xu, Shaozhuo</creatorcontrib><creatorcontrib>Liu, Dongliang</creatorcontrib><creatorcontrib>Ye, Zhicheng</creatorcontrib><creatorcontrib>Lin, Jing</creatorcontrib><creatorcontrib>Li, Xiaogang</creatorcontrib><creatorcontrib>Xue, Cheng</creatorcontrib><creatorcontrib>Wu, Jun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xue, Yongsong</au><au>Shan, Yanfei</au><au>Yao, Jia-Long</au><au>Wang, Runze</au><au>Xu, Shaozhuo</au><au>Liu, Dongliang</au><au>Ye, Zhicheng</au><au>Lin, Jing</au><au>Li, Xiaogang</au><au>Xue, Cheng</au><au>Wu, Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The transcription factor PbrMYB24 regulates lignin and cellulose biosynthesis in stone cells of pear fruits</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2023-07-03</date><risdate>2023</risdate><volume>192</volume><issue>3</issue><spage>1997</spage><epage>2014</epage><pages>1997-2014</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><abstract>Abstract
Lignified stone cell content is a key factor used to evaluate fruit quality, influencing the economic value of pear (Pyrus pyrifolia) fruits. However, our understanding of the regulatory networks of stone cell formation is limited due to the complex secondary metabolic pathway. In this study, we used a combination of co-expression network analysis, gene expression profiles, and transcriptome analysis in different pear cultivars with varied stone cell content to identify a hub MYB gene, PbrMYB24. The relative expression of PbrMYB24 in fruit flesh was significantly correlated with the contents of stone cells, lignin, and cellulose. We then verified the function of PbrMYB24 in regulating lignin and cellulose formation via genetic transformation in homologous and heterologous systems. We constructed a high-efficiency verification system for lignin and cellulose biosynthesis genes in pear callus. PbrMYB24 transcriptionally activated multiple target genes involved in stone cell formation. On the one hand, PbrMYB24 activated the transcription of lignin and cellulose biosynthesis genes by binding to different cis-elements [AC-I (ACCTACC) element, AC-II (ACCAACC) element and MYB-binding sites (MBS)]. On the other hand, PbrMYB24 bound directly to the promoters of PbrMYB169 and NAC STONE CELL PROMOTING FACTOR (PbrNSC), activating the gene expression. Moreover, both PbrMYB169 and PbrNSC activated the promoter of PbrMYB24, enhancing gene expression. This study improves our understanding of lignin and cellulose synthesis regulation in pear fruits through identifying a regulator and establishing a regulatory network. This knowledge will be useful for reducing the stone cell content in pears via molecular breeding.
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Fruit - genetics Fruit - metabolism Gene Expression Regulation, Plant Lignin - metabolism Pyrus - genetics Pyrus - metabolism Transcription Factors - metabolism |
| title | The transcription factor PbrMYB24 regulates lignin and cellulose biosynthesis in stone cells of pear fruits |
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