The In-Cell Western immunofluorescence assay to monitor PROTAC mediated protein degradation

The In-Cell Western plate-based immunofluorescence assay is a useful methodology for monitoring protein levels and provides a facile moderate through-put method for PROTAC and degrader optimization. The method is compared to other reported assays used for PROTAC development. The advantages of this m...

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Veröffentlicht in:	Methods in enzymology 2023-01, Vol.681, p.115-153
Hauptverfasser:	Lu, Lily D, Salvino, Joseph M
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line, Tumor Proteins Proteolysis
Online-Zugang:	Volltext
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