Paracatalytic induction: Subverting specificity in hedgehog protein autoprocessing with small molecules

Paracatalytic inducers are antagonists that shift the specificity of biological catalysts, resulting in non-native transformations. In this Chapter we describe methods to discover paracatalytic inducers of Hedgehog (Hh) protein autoprocessing. Native autoprocessing uses cholesterol as a substrate nu...

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Veröffentlicht in:	Methods in enzymology 2023, Vol.685, p.1-41
Hauptverfasser:	Ciulla, Daniel A, Xu, Zihan, Pezzullo, John L, Dranchak, Patricia, Wang, Chunyu, Giner, José-Luis, Inglese, James, Callahan, Brian P
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Catalysis Cholesterol - metabolism Drosophila - metabolism Drosophila Proteins - chemistry Hedgehog Proteins - chemistry Hedgehog Proteins - genetics Hedgehog Proteins - metabolism Humans
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