Sperm selection by birefringence: a promising non-invasive tool to improve ICSI outcomes
Despite higher sperm DNA fragmentation may affect intracytoplasmic sperm injection (ICSI) outcomes, sperm selection protocols do not evaluate this parameter. Therefore, sperm's head birefringence has been suggested as an adjuvant of seminal processing to select viable sperm for couples with sev...
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| Veröffentlicht in: | JBRA assisted reproduction 2023, Vol.27 (2), p.234-240 |
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| container_title | JBRA assisted reproduction |
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| creator | Ribeiro, Mariana Antunes Broi, Michele Gomes Da Rose, Mauro Bibancos de Garolla, Andrea Foresta, Carlo Bragheto, Aristides Manoel Dos Santos Hardy, Daniel Gustavo Faúndes |
| description | Despite higher sperm DNA fragmentation may affect intracytoplasmic sperm injection (ICSI) outcomes, sperm selection protocols do not evaluate this parameter. Therefore, sperm's head birefringence has been suggested as an adjuvant of seminal processing to select viable sperm for couples with severe male factor. Considering men with normal seminal parameters may also curse with DNA fragmentation, the aim of this study was to evaluate the impact of sperm selection by birefringence on ICSI outcomes in couples with different infertility factors compared to those submitted to conventional sperm selection.
In this case-control study, medical records from 181 couples who underwent ICSI from January 2018 to August 2020 (107 from the Conventional and 74 from the Birefringence group) were included in the study. Clinical characteristics and ICSI outcomes were compared between the groups using Student's t test or Chi-square test (p |
| doi_str_mv | 10.5935/1518-0557.20220055 |
| format | Article |
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In this case-control study, medical records from 181 couples who underwent ICSI from January 2018 to August 2020 (107 from the Conventional and 74 from the Birefringence group) were included in the study. Clinical characteristics and ICSI outcomes were compared between the groups using Student's t test or Chi-square test (p<0.05) and a multivariate logistic regression model was applied regarding clinical pregnancy.
Despite the Birefringence group showed higher female age (p=0.01), lower seminal sperm concentration (p<0.01) and higher sperm DNA fragmentation (p<0.01), those patients cursed with both higher cleavage rate (p=0.04), clinical pregnancy rate per transfer (p=0.03) and clinical pregnancy rate per initiated cycle (p=0.02). The logistic regression showed a positive group effect on clinical pregnancy.
The findings suggest a positive clinical impact of this cheap and easily reproducible adjuvant technique on ICSI outcomes in couples with different infertility factors. If confirmed by further methodologically appropriate studies, the sperm's head birefringence could be considered to improve the reproductive chances of those patients.</description><identifier>ISSN: 1518-0557</identifier><identifier>ISSN: 1517-5693</identifier><identifier>EISSN: 1518-0557</identifier><identifier>DOI: 10.5935/1518-0557.20220055</identifier><identifier>PMID: 36468799</identifier><language>eng</language><publisher>Brazil: Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)</publisher><subject>Embryos ; Genetic testing ; Infertility ; Morphology ; Motility ; Original ; Ovaries ; Pregnancy ; Sperm ; Ultrasonic imaging</subject><ispartof>JBRA assisted reproduction, 2023, Vol.27 (2), p.234-240</ispartof><rights>2023. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-275a1f39af755002f4b875274c1cd438f352e73c64bdd3e0a903a93cc30912c53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279423/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10279423/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,4010,27904,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36468799$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ribeiro, Mariana Antunes</creatorcontrib><creatorcontrib>Broi, Michele Gomes Da</creatorcontrib><creatorcontrib>Rose, Mauro Bibancos de</creatorcontrib><creatorcontrib>Garolla, Andrea</creatorcontrib><creatorcontrib>Foresta, Carlo</creatorcontrib><creatorcontrib>Bragheto, Aristides Manoel Dos Santos</creatorcontrib><creatorcontrib>Hardy, Daniel Gustavo Faúndes</creatorcontrib><title>Sperm selection by birefringence: a promising non-invasive tool to improve ICSI outcomes</title><title>JBRA assisted reproduction</title><addtitle>JBRA Assist Reprod</addtitle><description>Despite higher sperm DNA fragmentation may affect intracytoplasmic sperm injection (ICSI) outcomes, sperm selection protocols do not evaluate this parameter. Therefore, sperm's head birefringence has been suggested as an adjuvant of seminal processing to select viable sperm for couples with severe male factor. Considering men with normal seminal parameters may also curse with DNA fragmentation, the aim of this study was to evaluate the impact of sperm selection by birefringence on ICSI outcomes in couples with different infertility factors compared to those submitted to conventional sperm selection.
In this case-control study, medical records from 181 couples who underwent ICSI from January 2018 to August 2020 (107 from the Conventional and 74 from the Birefringence group) were included in the study. Clinical characteristics and ICSI outcomes were compared between the groups using Student's t test or Chi-square test (p<0.05) and a multivariate logistic regression model was applied regarding clinical pregnancy.
Despite the Birefringence group showed higher female age (p=0.01), lower seminal sperm concentration (p<0.01) and higher sperm DNA fragmentation (p<0.01), those patients cursed with both higher cleavage rate (p=0.04), clinical pregnancy rate per transfer (p=0.03) and clinical pregnancy rate per initiated cycle (p=0.02). The logistic regression showed a positive group effect on clinical pregnancy.
The findings suggest a positive clinical impact of this cheap and easily reproducible adjuvant technique on ICSI outcomes in couples with different infertility factors. If confirmed by further methodologically appropriate studies, the sperm's head birefringence could be considered to improve the reproductive chances of those patients.</description><subject>Embryos</subject><subject>Genetic testing</subject><subject>Infertility</subject><subject>Morphology</subject><subject>Motility</subject><subject>Original</subject><subject>Ovaries</subject><subject>Pregnancy</subject><subject>Sperm</subject><subject>Ultrasonic imaging</subject><issn>1518-0557</issn><issn>1517-5693</issn><issn>1518-0557</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNpdUU1LAzEQDaKoqH_AgwS8eFmdfDW7XkSKHwXBgwreQjbN1pTdpCa7Bf-9KbVFvUwmM28e8-YhdErgUlRMXBFBygKEkJcUKIWc7aDDbXH3V36ATlKaAwCpCGUc9tEBG_FRKavqEL2_LGzscLKtNb0LHtdfuHbRNtH5mfXGXmONFzF0LuUC9sEXzi91ckuL-xDaHLDrMiD_J-OXCQ5Db0Jn0zHaa3Sb7MnPe4Te7u9ex4_F0_PDZHz7VBjOSF9QKTRpWKUbKQQAbXhdSkElN8RMOSsbJqiVzIx4PZ0yC7oCpitmDIOsxgh2hG7WvIuh7uzUWN9H3apFdJ2OXypop_52vPtQs7BUBKisOGWZ4eKHIYbPwaZeZbHGtq32NgxJ5WUkAM_3y9Dzf9B5GKLP-hQtGZElELJC0TXKxJBSvuV2GwJqZZ5aeaNW3qiNeXno7LeO7cjGKvYNvmaUJg</recordid><startdate>2023</startdate><enddate>2023</enddate><creator>Ribeiro, Mariana Antunes</creator><creator>Broi, Michele Gomes Da</creator><creator>Rose, Mauro Bibancos de</creator><creator>Garolla, Andrea</creator><creator>Foresta, Carlo</creator><creator>Bragheto, Aristides Manoel Dos Santos</creator><creator>Hardy, Daniel Gustavo Faúndes</creator><general>Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)</general><general>Brazilian Society of Assisted Reproduction</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>2023</creationdate><title>Sperm selection by birefringence: a promising non-invasive tool to improve ICSI outcomes</title><author>Ribeiro, Mariana Antunes ; Broi, Michele Gomes Da ; Rose, Mauro Bibancos de ; Garolla, Andrea ; Foresta, Carlo ; Bragheto, Aristides Manoel Dos Santos ; Hardy, Daniel Gustavo Faúndes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-275a1f39af755002f4b875274c1cd438f352e73c64bdd3e0a903a93cc30912c53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Embryos</topic><topic>Genetic testing</topic><topic>Infertility</topic><topic>Morphology</topic><topic>Motility</topic><topic>Original</topic><topic>Ovaries</topic><topic>Pregnancy</topic><topic>Sperm</topic><topic>Ultrasonic imaging</topic><toplevel>online_resources</toplevel><creatorcontrib>Ribeiro, Mariana Antunes</creatorcontrib><creatorcontrib>Broi, Michele Gomes Da</creatorcontrib><creatorcontrib>Rose, Mauro Bibancos de</creatorcontrib><creatorcontrib>Garolla, Andrea</creatorcontrib><creatorcontrib>Foresta, Carlo</creatorcontrib><creatorcontrib>Bragheto, Aristides Manoel Dos Santos</creatorcontrib><creatorcontrib>Hardy, Daniel Gustavo Faúndes</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>JBRA assisted reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ribeiro, Mariana Antunes</au><au>Broi, Michele Gomes Da</au><au>Rose, Mauro Bibancos de</au><au>Garolla, Andrea</au><au>Foresta, Carlo</au><au>Bragheto, Aristides Manoel Dos Santos</au><au>Hardy, Daniel Gustavo Faúndes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sperm selection by birefringence: a promising non-invasive tool to improve ICSI outcomes</atitle><jtitle>JBRA assisted reproduction</jtitle><addtitle>JBRA Assist Reprod</addtitle><date>2023</date><risdate>2023</risdate><volume>27</volume><issue>2</issue><spage>234</spage><epage>240</epage><pages>234-240</pages><issn>1518-0557</issn><issn>1517-5693</issn><eissn>1518-0557</eissn><abstract>Despite higher sperm DNA fragmentation may affect intracytoplasmic sperm injection (ICSI) outcomes, sperm selection protocols do not evaluate this parameter. Therefore, sperm's head birefringence has been suggested as an adjuvant of seminal processing to select viable sperm for couples with severe male factor. Considering men with normal seminal parameters may also curse with DNA fragmentation, the aim of this study was to evaluate the impact of sperm selection by birefringence on ICSI outcomes in couples with different infertility factors compared to those submitted to conventional sperm selection.
In this case-control study, medical records from 181 couples who underwent ICSI from January 2018 to August 2020 (107 from the Conventional and 74 from the Birefringence group) were included in the study. Clinical characteristics and ICSI outcomes were compared between the groups using Student's t test or Chi-square test (p<0.05) and a multivariate logistic regression model was applied regarding clinical pregnancy.
Despite the Birefringence group showed higher female age (p=0.01), lower seminal sperm concentration (p<0.01) and higher sperm DNA fragmentation (p<0.01), those patients cursed with both higher cleavage rate (p=0.04), clinical pregnancy rate per transfer (p=0.03) and clinical pregnancy rate per initiated cycle (p=0.02). The logistic regression showed a positive group effect on clinical pregnancy.
The findings suggest a positive clinical impact of this cheap and easily reproducible adjuvant technique on ICSI outcomes in couples with different infertility factors. If confirmed by further methodologically appropriate studies, the sperm's head birefringence could be considered to improve the reproductive chances of those patients.</abstract><cop>Brazil</cop><pub>Sociedade Brasileira de Reprodução Humana (Brazilian Society of Assisted Reproduction)</pub><pmid>36468799</pmid><doi>10.5935/1518-0557.20220055</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | JBRA assisted reproduction, 2023, Vol.27 (2), p.234-240 |
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| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Embryos Genetic testing Infertility Morphology Motility Original Ovaries Pregnancy Sperm Ultrasonic imaging |
| title | Sperm selection by birefringence: a promising non-invasive tool to improve ICSI outcomes |
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