High-risk human papillomavirus in Turkish patients with clinically suspicious cervical lesions analyzed by multiplex-PCR
Human papillomavirus (HPV) infection is known to be the main cause of cervical cancer. This study aimed to determine the prevalence of high-risk HPV genotypes in smear specimens taken from women who had normal or abnormal cytology using a multiplex PCR method. The study included 270 women aged betwe...
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| Veröffentlicht in: | Indian journal of medical research (New Delhi, India : 1994) India : 1994), 2022-12, Vol.156 (6), p.786-791 |
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| container_title | Indian journal of medical research (New Delhi, India : 1994) |
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| creator | Gorur, Levent Dolanbay, Mehmet Ozturk, Figen Canoz, Ozlem Donmez-Altuntas, Hamiyet |
| description | Human papillomavirus (HPV) infection is known to be the main cause of cervical cancer. This study aimed to determine the prevalence of high-risk HPV genotypes in smear specimens taken from women who had normal or abnormal cytology using a multiplex PCR method.
The study included 270 women aged between 19 and 69 yr with or without suspicious cervical abnormalities. A Pap smear sample from each patient was cytologically examined, and HPV typing was performed using a multiplex fluorescent PCR method. Those who were high-risk HPV positive and had a normal or abnormal cytology were further evaluated by colposcopy and biopsy.
The total HPV positivity was 43 per cent (116/270). HPV positivity in the patients with an abnormal cytology was 77 per cent (33/43), whereas it was only 37 per cent (83/227) in women with normal cytology, which showed a significant difference (P |
| doi_str_mv | 10.4103/ijmr.IJMR_2335_20 |
| format | Article |
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The study included 270 women aged between 19 and 69 yr with or without suspicious cervical abnormalities. A Pap smear sample from each patient was cytologically examined, and HPV typing was performed using a multiplex fluorescent PCR method. Those who were high-risk HPV positive and had a normal or abnormal cytology were further evaluated by colposcopy and biopsy.
The total HPV positivity was 43 per cent (116/270). HPV positivity in the patients with an abnormal cytology was 77 per cent (33/43), whereas it was only 37 per cent (83/227) in women with normal cytology, which showed a significant difference (P<0.05). HPV positivity was also related to the age group when all the subjects were considered (P<0.05), and the highest prevalence of HPV infection was in the 30-39 yr age group. High-risk HPV types 16, 18, 31, 35, 51 and 56 were more common in the normal cytology patients, whereas high-risk HPV types 16, 31, 35, 45, 58 and 68 were commonly found in the abnormal cytology patients.
The determination of high-risk HPV genotypes in women with clinically suspicious cervical lesions should be conducted during an annual follow-up, irrespective of a normal or abnormal cytology by the age of 30 years or above.</description><identifier>ISSN: 0971-5916</identifier><identifier>EISSN: 0975-9174</identifier><identifier>DOI: 10.4103/ijmr.IJMR_2335_20</identifier><identifier>PMID: 37056079</identifier><language>eng</language><publisher>India: Wolters Kluwer - Medknow</publisher><subject>Adult ; Aged ; Colposcopy ; Female ; Human Papillomavirus Viruses ; Humans ; Middle Aged ; Multiplex Polymerase Chain Reaction ; Papanicolaou Test ; Papillomaviridae - genetics ; Papillomavirus Infections - epidemiology ; Papillomavirus Infections - genetics ; Practice: Original article ; Pregnancy ; Uterine Cervical Neoplasms - pathology ; Vaginal Smears ; Young Adult</subject><ispartof>Indian journal of medical research (New Delhi, India : 1994), 2022-12, Vol.156 (6), p.786-791</ispartof><rights>Copyright: © 2023 Indian Journal of Medical Research 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278910/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC10278910/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37056079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gorur, Levent</creatorcontrib><creatorcontrib>Dolanbay, Mehmet</creatorcontrib><creatorcontrib>Ozturk, Figen</creatorcontrib><creatorcontrib>Canoz, Ozlem</creatorcontrib><creatorcontrib>Donmez-Altuntas, Hamiyet</creatorcontrib><title>High-risk human papillomavirus in Turkish patients with clinically suspicious cervical lesions analyzed by multiplex-PCR</title><title>Indian journal of medical research (New Delhi, India : 1994)</title><addtitle>Indian J Med Res</addtitle><description>Human papillomavirus (HPV) infection is known to be the main cause of cervical cancer. This study aimed to determine the prevalence of high-risk HPV genotypes in smear specimens taken from women who had normal or abnormal cytology using a multiplex PCR method.
The study included 270 women aged between 19 and 69 yr with or without suspicious cervical abnormalities. A Pap smear sample from each patient was cytologically examined, and HPV typing was performed using a multiplex fluorescent PCR method. Those who were high-risk HPV positive and had a normal or abnormal cytology were further evaluated by colposcopy and biopsy.
The total HPV positivity was 43 per cent (116/270). HPV positivity in the patients with an abnormal cytology was 77 per cent (33/43), whereas it was only 37 per cent (83/227) in women with normal cytology, which showed a significant difference (P<0.05). HPV positivity was also related to the age group when all the subjects were considered (P<0.05), and the highest prevalence of HPV infection was in the 30-39 yr age group. High-risk HPV types 16, 18, 31, 35, 51 and 56 were more common in the normal cytology patients, whereas high-risk HPV types 16, 31, 35, 45, 58 and 68 were commonly found in the abnormal cytology patients.
The determination of high-risk HPV genotypes in women with clinically suspicious cervical lesions should be conducted during an annual follow-up, irrespective of a normal or abnormal cytology by the age of 30 years or above.</description><subject>Adult</subject><subject>Aged</subject><subject>Colposcopy</subject><subject>Female</subject><subject>Human Papillomavirus Viruses</subject><subject>Humans</subject><subject>Middle Aged</subject><subject>Multiplex Polymerase Chain Reaction</subject><subject>Papanicolaou Test</subject><subject>Papillomaviridae - genetics</subject><subject>Papillomavirus Infections - epidemiology</subject><subject>Papillomavirus Infections - genetics</subject><subject>Practice: Original article</subject><subject>Pregnancy</subject><subject>Uterine Cervical Neoplasms - pathology</subject><subject>Vaginal Smears</subject><subject>Young Adult</subject><issn>0971-5916</issn><issn>0975-9174</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkMtOwzAQRS0EolD4ADbIP5Bi52HXK4QqoEVFoKqsI9txmmmdh-ykNHw95SlYzWjOnSvdi9AFJaOYkugK1qUbzR4eF2kYRUkakgN0QgRPAkF5fPi50yARlA3QqfdrQqgIuThGg4iThBEuTtBuCqsicOA3uOhKWeFGNmBtXcotuM5jqPCycxvwxZ60YKrW41doC6wtVKCltT32nW9AQ72Xa-O2H1dsjYe68lhW0vZvJsOqx2VnW2is2QXPk8UZOsql9eb8ew7Ry93tcjIN5k_3s8nNPGgoi9sgEkSrPM9kLDIdGpqIsTIy44yEsYoVyWPGleJc6pyGGeNCEMNYEmeKjHMm82iIrr98m06VJtP7BE7atHFQStentYT0P6mgSFf1NqUk5GOxr3mILv86_L7-lBi9A7RTeyU</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Gorur, Levent</creator><creator>Dolanbay, Mehmet</creator><creator>Ozturk, Figen</creator><creator>Canoz, Ozlem</creator><creator>Donmez-Altuntas, Hamiyet</creator><general>Wolters Kluwer - Medknow</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>5PM</scope></search><sort><creationdate>20221201</creationdate><title>High-risk human papillomavirus in Turkish patients with clinically suspicious cervical lesions analyzed by multiplex-PCR</title><author>Gorur, Levent ; Dolanbay, Mehmet ; Ozturk, Figen ; Canoz, Ozlem ; Donmez-Altuntas, Hamiyet</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p164t-390cbffda49dc2e1598bead76024b4b0f467bb77acf12d67990e6654db08f6af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Colposcopy</topic><topic>Female</topic><topic>Human Papillomavirus Viruses</topic><topic>Humans</topic><topic>Middle Aged</topic><topic>Multiplex Polymerase Chain Reaction</topic><topic>Papanicolaou Test</topic><topic>Papillomaviridae - genetics</topic><topic>Papillomavirus Infections - epidemiology</topic><topic>Papillomavirus Infections - genetics</topic><topic>Practice: Original article</topic><topic>Pregnancy</topic><topic>Uterine Cervical Neoplasms - pathology</topic><topic>Vaginal Smears</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gorur, Levent</creatorcontrib><creatorcontrib>Dolanbay, Mehmet</creatorcontrib><creatorcontrib>Ozturk, Figen</creatorcontrib><creatorcontrib>Canoz, Ozlem</creatorcontrib><creatorcontrib>Donmez-Altuntas, Hamiyet</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Indian journal of medical research (New Delhi, India : 1994)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gorur, Levent</au><au>Dolanbay, Mehmet</au><au>Ozturk, Figen</au><au>Canoz, Ozlem</au><au>Donmez-Altuntas, Hamiyet</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-risk human papillomavirus in Turkish patients with clinically suspicious cervical lesions analyzed by multiplex-PCR</atitle><jtitle>Indian journal of medical research (New Delhi, India : 1994)</jtitle><addtitle>Indian J Med Res</addtitle><date>2022-12-01</date><risdate>2022</risdate><volume>156</volume><issue>6</issue><spage>786</spage><epage>791</epage><pages>786-791</pages><issn>0971-5916</issn><eissn>0975-9174</eissn><abstract>Human papillomavirus (HPV) infection is known to be the main cause of cervical cancer. This study aimed to determine the prevalence of high-risk HPV genotypes in smear specimens taken from women who had normal or abnormal cytology using a multiplex PCR method.
The study included 270 women aged between 19 and 69 yr with or without suspicious cervical abnormalities. A Pap smear sample from each patient was cytologically examined, and HPV typing was performed using a multiplex fluorescent PCR method. Those who were high-risk HPV positive and had a normal or abnormal cytology were further evaluated by colposcopy and biopsy.
The total HPV positivity was 43 per cent (116/270). HPV positivity in the patients with an abnormal cytology was 77 per cent (33/43), whereas it was only 37 per cent (83/227) in women with normal cytology, which showed a significant difference (P<0.05). HPV positivity was also related to the age group when all the subjects were considered (P<0.05), and the highest prevalence of HPV infection was in the 30-39 yr age group. High-risk HPV types 16, 18, 31, 35, 51 and 56 were more common in the normal cytology patients, whereas high-risk HPV types 16, 31, 35, 45, 58 and 68 were commonly found in the abnormal cytology patients.
The determination of high-risk HPV genotypes in women with clinically suspicious cervical lesions should be conducted during an annual follow-up, irrespective of a normal or abnormal cytology by the age of 30 years or above.</abstract><cop>India</cop><pub>Wolters Kluwer - Medknow</pub><pmid>37056079</pmid><doi>10.4103/ijmr.IJMR_2335_20</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Medknow Open Access Medical Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Adult Aged Colposcopy Female Human Papillomavirus Viruses Humans Middle Aged Multiplex Polymerase Chain Reaction Papanicolaou Test Papillomaviridae - genetics Papillomavirus Infections - epidemiology Papillomavirus Infections - genetics Practice: Original article Pregnancy Uterine Cervical Neoplasms - pathology Vaginal Smears Young Adult |
| title | High-risk human papillomavirus in Turkish patients with clinically suspicious cervical lesions analyzed by multiplex-PCR |
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